SG11202108563QA - Methods for nucleic acid analysis - Google Patents

Methods for nucleic acid analysis

Info

Publication number
SG11202108563QA
SG11202108563QA SG11202108563QA SG11202108563QA SG11202108563QA SG 11202108563Q A SG11202108563Q A SG 11202108563QA SG 11202108563Q A SG11202108563Q A SG 11202108563QA SG 11202108563Q A SG11202108563Q A SG 11202108563QA SG 11202108563Q A SG11202108563Q A SG 11202108563QA
Authority
SG
Singapore
Prior art keywords
methods
nucleic acid
acid analysis
analysis
nucleic
Prior art date
Application number
SG11202108563QA
Other languages
English (en)
Inventor
Gilad Almogy
Florian Oberstrass
Omer Barad
Chandan Shee
Original Assignee
Ultima Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ultima Genomics Inc filed Critical Ultima Genomics Inc
Publication of SG11202108563QA publication Critical patent/SG11202108563QA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202108563QA 2019-02-11 2020-02-10 Methods for nucleic acid analysis SG11202108563QA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962804082P 2019-02-11 2019-02-11
US201962890240P 2019-08-22 2019-08-22
US201962916683P 2019-10-17 2019-10-17
PCT/US2020/017491 WO2020167656A1 (en) 2019-02-11 2020-02-10 Methods for nucleic acid analysis

Publications (1)

Publication Number Publication Date
SG11202108563QA true SG11202108563QA (en) 2021-09-29

Family

ID=72045127

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202108563QA SG11202108563QA (en) 2019-02-11 2020-02-10 Methods for nucleic acid analysis

Country Status (10)

Country Link
US (1) US12319959B2 (enrdf_load_stackoverflow)
EP (1) EP3924515A4 (enrdf_load_stackoverflow)
JP (1) JP7528103B2 (enrdf_load_stackoverflow)
KR (1) KR20210138017A (enrdf_load_stackoverflow)
CN (1) CN113728113A (enrdf_load_stackoverflow)
AU (1) AU2020222888B2 (enrdf_load_stackoverflow)
CA (1) CA3129315A1 (enrdf_load_stackoverflow)
MX (1) MX2021009482A (enrdf_load_stackoverflow)
SG (1) SG11202108563QA (enrdf_load_stackoverflow)
WO (1) WO2020167656A1 (enrdf_load_stackoverflow)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2021009482A (es) 2019-02-11 2021-11-12 Ultima Genomics Inc Metodos para el analisis de acidos nucleicos.
KR20230052952A (ko) * 2020-08-21 2023-04-20 울티마 제노믹스, 인크. 표면 증폭을 위한 조성물 및 이의 용도
AU2022328558A1 (en) 2021-08-20 2024-04-04 Ultima Genomics, Inc. Systems and methods for sample preparation for sequencing
WO2023081883A2 (en) * 2021-11-08 2023-05-11 Ultima Genomics, Inc. Methylation sequencing methods and compositions
WO2025072163A1 (en) * 2023-09-25 2025-04-03 Ultima Genomics, Inc. Systems and methods for processing analytes

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9388459B2 (en) 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
EP1590477B1 (en) * 2003-01-29 2009-07-29 454 Corporation Methods of amplifying and sequencing nucleic acids
WO2005010145A2 (en) * 2003-07-05 2005-02-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
CN1950519A (zh) 2004-02-27 2007-04-18 哈佛大学的校长及成员们 聚合酶群落荧光原位测序珠子
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
WO2013158982A1 (en) 2012-04-19 2013-10-24 Life Technologies Corporation Method of performing digital pcr
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
EP3147374B1 (en) 2010-12-17 2019-01-30 Life Technologies Corporation Methods for nucleic acid amplification
LT2742151T (lt) 2011-08-10 2018-02-12 Life Technologies Corporation Polimerazės kompozicija
EP3095879B1 (en) 2012-04-19 2018-09-19 Life Technologies Corporation Nucleic acid amplification
CN116064734A (zh) 2012-04-19 2023-05-05 生命技术公司 核酸扩增
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
US10066260B2 (en) 2013-03-14 2018-09-04 Life Technologies Corporation Matrix arrays and methods for making same
CA2905505C (en) * 2013-03-15 2023-03-14 Lineage Biosciences, Inc. Methods of characterizing the immune repertoire by tagging and sequencing immunoglobulin or t-cell receptor nucleic acids
US10160995B2 (en) 2013-05-13 2018-12-25 Qiagen Waltham, Inc. Analyte enrichment methods and compositions
BR112015032512A8 (pt) * 2013-06-27 2020-01-07 10X Genomics Inc método de implementação de código de barras de materiais de amostra
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
WO2015191815A1 (en) 2014-06-13 2015-12-17 Life Technologies Corporation Multiplex nucleic acid amplification
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
WO2016187466A1 (en) * 2015-05-20 2016-11-24 Invenra, Inc. Methods, apparatus and compositions for expression and high throughput screening
EP3365445B1 (en) 2015-10-19 2023-05-31 Dovetail Genomics, LLC Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
AU2016348439B2 (en) * 2015-11-04 2023-03-09 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US10730030B2 (en) * 2016-01-08 2020-08-04 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
EP3440220B1 (en) 2016-04-07 2020-03-25 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
WO2018017884A1 (en) 2016-07-20 2018-01-25 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2018064297A1 (en) 2016-09-29 2018-04-05 Ultima Genomics, Inc. Systems and methods for nucleic acid sequencing
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP2018183097A (ja) 2017-04-26 2018-11-22 株式会社エンプラス 細胞由来の解析用液滴の単離方法、および細胞の解析方法
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2019079653A1 (en) 2017-10-19 2019-04-25 Ultima Genomics, Inc. METHODS OF TREATING APPARATUSED END SEQUENCES
EP3707274B1 (en) 2017-11-07 2023-09-13 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
US12226745B2 (en) 2017-11-07 2025-02-18 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
SG11202007686VA (en) * 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
MX2021009482A (es) 2019-02-11 2021-11-12 Ultima Genomics Inc Metodos para el analisis de acidos nucleicos.
WO2020172197A1 (en) 2019-02-19 2020-08-27 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
KR20230052952A (ko) 2020-08-21 2023-04-20 울티마 제노믹스, 인크. 표면 증폭을 위한 조성물 및 이의 용도
EP4320274A4 (en) 2021-04-09 2025-03-05 Ultima Genomics, Inc. SYSTEMS AND METHODS FOR SPATIAL SCREENING OF ANALYTES

Also Published As

Publication number Publication date
EP3924515A1 (en) 2021-12-22
CN113728113A (zh) 2021-11-30
KR20210138017A (ko) 2021-11-18
JP2022519324A (ja) 2022-03-22
MX2021009482A (es) 2021-11-12
EP3924515A4 (en) 2022-08-24
JP7528103B2 (ja) 2024-08-05
US20220042072A1 (en) 2022-02-10
AU2020222888A1 (en) 2021-09-30
WO2020167656A1 (en) 2020-08-20
US12319959B2 (en) 2025-06-03
CA3129315A1 (en) 2020-08-20
AU2020222888B2 (en) 2024-08-01

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