SG11202107383RA - Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois - Google Patents
Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyeloisInfo
- Publication number
- SG11202107383RA SG11202107383RA SG11202107383RA SG11202107383RA SG11202107383RA SG 11202107383R A SG11202107383R A SG 11202107383RA SG 11202107383R A SG11202107383R A SG 11202107383RA SG 11202107383R A SG11202107383R A SG 11202107383RA SG 11202107383R A SG11202107383R A SG 11202107383RA
- Authority
- SG
- Singapore
- Prior art keywords
- amyelois
- transposase
- transposition
- nucleic acid
- acid constructs
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/007—Vector systems having a special element relevant for transcription cell cycle specific enhancer/promoter combination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Ticket-Dispensing Machines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962831097P | 2019-04-08 | 2019-04-08 | |
| US201962873342P | 2019-07-12 | 2019-07-12 | |
| US202062990568P | 2020-03-17 | 2020-03-17 | |
| PCT/US2020/027078 WO2020210237A1 (en) | 2019-04-08 | 2020-04-07 | Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202107383RA true SG11202107383RA (en) | 2021-08-30 |
Family
ID=72663758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202107383RA SG11202107383RA (en) | 2019-04-08 | 2020-04-07 | Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US11060109B2 (de) |
| EP (1) | EP3953479A4 (de) |
| JP (1) | JP7418469B2 (de) |
| KR (1) | KR20210144861A (de) |
| CN (2) | CN118620926A (de) |
| AU (1) | AU2020272667B2 (de) |
| BR (1) | BR112021020144A2 (de) |
| CA (1) | CA3125043A1 (de) |
| IL (2) | IL287088B2 (de) |
| SG (1) | SG11202107383RA (de) |
| WO (1) | WO2020210237A1 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112424362B (zh) * | 2019-04-08 | 2024-07-23 | Dna2.0股份有限公司 | 使用来自青鳉属的转座酶将核酸构建体整合入真核细胞 |
| WO2020210236A1 (en) | 2019-04-08 | 2020-10-15 | Dna Twopointo Inc. | Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis |
| JP7418469B2 (ja) | 2019-04-08 | 2024-01-19 | ディーエヌエー ツーポイントオー インク． | アミエロイス由来のトランスポザーゼを用いた核酸コンストラクトの真核生物のゲノムへの転移 |
| EP4146798A1 (de) | 2020-05-04 | 2023-03-15 | Saliogen Therapeutics, Inc. | Transpositionsbasierte therapien |
| EP4314302A1 (de) * | 2021-03-24 | 2024-02-07 | Dna Twopointo Inc. | Tetracyclininduzierbare expressionssysteme |
| CN116445542A (zh) * | 2022-01-10 | 2023-07-18 | 佛山汉腾生物科技有限公司 | 表达载体及其应用 |
| WO2024068995A1 (en) | 2022-09-30 | 2024-04-04 | Boehringer Ingelheim International Gmbh | Novel transposase system |
| CN116590341A (zh) * | 2023-07-13 | 2023-08-15 | 山东维真生物科技有限公司 | 一种腺病毒-PiggyBac系统及其制备方法和应用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7560542B2 (en) | 1999-05-07 | 2009-07-14 | Monsanto Technology Llc | Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants |
| US6518064B1 (en) * | 2000-03-29 | 2003-02-11 | The Regents Of The University Of California | Pink bollworm expression system for commercially valuable protein production |
| US7932088B1 (en) | 2005-04-25 | 2011-04-26 | University Of Notre Dame Du Lac | High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac |
| US20100154070A1 (en) * | 2005-05-14 | 2010-06-17 | Tian Xu | PiggyBac as a Tool for Genetic Manipulation and Analysis in Vertebrates |
| CA2750027C (en) | 2009-01-23 | 2020-11-10 | Nsgene A/S | Improved cell lines and their use in encapsulated cell biodelivery |
| SG174155A1 (en) | 2009-02-25 | 2011-10-28 | Univ Johns Hopkins | Piggybac transposon variants and methods of use |
| KR101812348B1 (ko) * | 2009-06-11 | 2017-12-26 | 다이가쿠쿄도리요우키칸호우진 죠우호우시스테무 겡큐키코우 | 단백질의 생산 방법 |
| EA201391234A1 (ru) * | 2011-02-25 | 2014-05-30 | Рикомбинетикс, Инк. | Генетически модифицированные животные и способы их получения |
| SI2683419T1 (sl) * | 2011-03-11 | 2018-10-30 | Vib Vzw | Molekule in postopki za blokiranje in zaznavanje beljakovin |
| PL2992101T3 (pl) * | 2013-04-29 | 2019-05-31 | Agrosavfe Nv | Kompozycje agrochemiczne zawierające przeciwciała wiążące się ze sfingolipidami |
| US10344285B2 (en) * | 2014-04-09 | 2019-07-09 | Dna2.0, Inc. | DNA vectors, transposons and transposases for eukaryotic genome modification |
| WO2015157579A2 (en) * | 2014-04-09 | 2015-10-15 | Dna2.0, Inc. | Enhanced nucleic acid constructs for eukaryotic gene expression |
| DK3359671T3 (da) | 2015-10-08 | 2021-09-20 | Dna Twopointo Inc | Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation |
| WO2020210236A1 (en) | 2019-04-08 | 2020-10-15 | Dna Twopointo Inc. | Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis |
| CN112424362B (zh) | 2019-04-08 | 2024-07-23 | Dna2.0股份有限公司 | 使用来自青鳉属的转座酶将核酸构建体整合入真核细胞 |
| JP7418469B2 (ja) * | 2019-04-08 | 2024-01-19 | ディーエヌエー ツーポイントオー インク． | アミエロイス由来のトランスポザーゼを用いた核酸コンストラクトの真核生物のゲノムへの転移 |
| US20230313187A1 (en) * | 2020-09-04 | 2023-10-05 | Dna Twopointo Inc. | Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products |
-
2020
- 2020-04-07 JP JP2021560152A patent/JP7418469B2/ja active Active
- 2020-04-07 IL IL287088A patent/IL287088B2/en unknown
- 2020-04-07 KR KR1020217035246A patent/KR20210144861A/ko not_active Application Discontinuation
- 2020-04-07 AU AU2020272667A patent/AU2020272667B2/en active Active
- 2020-04-07 CA CA3125043A patent/CA3125043A1/en active Pending
- 2020-04-07 CN CN202410890070.2A patent/CN118620926A/zh active Pending
- 2020-04-07 WO PCT/US2020/027078 patent/WO2020210237A1/en unknown
- 2020-04-07 US US16/842,709 patent/US11060109B2/en active Active
- 2020-04-07 SG SG11202107383RA patent/SG11202107383RA/en unknown
- 2020-04-07 BR BR112021020144A patent/BR112021020144A2/pt unknown
- 2020-04-07 IL IL301621A patent/IL301621A/en unknown
- 2020-04-07 EP EP20787187.2A patent/EP3953479A4/de active Pending
- 2020-04-07 CN CN202080003853.4A patent/CN112513277B/zh active Active
-
2021
- 2021-06-04 US US17/339,597 patent/US11396662B2/en active Active
-
2022
- 2022-06-22 US US17/847,021 patent/US20220411816A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20210144861A (ko) | 2021-11-30 |
| WO2020210237A1 (en) | 2020-10-15 |
| IL287088B1 (en) | 2023-05-01 |
| EP3953479A1 (de) | 2022-02-16 |
| CN112513277B (zh) | 2024-07-23 |
| US20210324408A1 (en) | 2021-10-21 |
| IL301621A (en) | 2023-05-01 |
| US11396662B2 (en) | 2022-07-26 |
| JP2022527016A (ja) | 2022-05-27 |
| US20200318135A1 (en) | 2020-10-08 |
| IL287088A (en) | 2021-12-01 |
| IL287088B2 (en) | 2023-09-01 |
| CN112513277A (zh) | 2021-03-16 |
| US11060109B2 (en) | 2021-07-13 |
| AU2020272667B2 (en) | 2023-07-06 |
| US20220411816A1 (en) | 2022-12-29 |
| BR112021020144A2 (pt) | 2021-12-14 |
| AU2020272667A1 (en) | 2021-07-15 |
| CN118620926A (zh) | 2024-09-10 |
| EP3953479A4 (de) | 2023-01-11 |
| JP7418469B2 (ja) | 2024-01-19 |
| CA3125043A1 (en) | 2020-10-15 |
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