SG11202100470WA - Engineered glycosyltransferases and steviol glycoside glucosylation methods - Google Patents

Engineered glycosyltransferases and steviol glycoside glucosylation methods

Info

Publication number
SG11202100470WA
SG11202100470WA SG11202100470WA SG11202100470WA SG11202100470WA SG 11202100470W A SG11202100470W A SG 11202100470WA SG 11202100470W A SG11202100470W A SG 11202100470WA SG 11202100470W A SG11202100470W A SG 11202100470WA SG 11202100470W A SG11202100470W A SG 11202100470WA
Authority
SG
Singapore
Prior art keywords
steviol glycoside
engineered glycosyltransferases
methods
glycoside glucosylation
glucosylation methods
Prior art date
Application number
SG11202100470WA
Other languages
English (en)
Inventor
Jonathan Vroom
Stephanie Galanie
Jack Liang
Joyce Liu
Nikki Dellas
Melissa Mayo
David Entwistle
Original Assignee
Codexis Inc
Tate & Lyle Ingredients Americas Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc, Tate & Lyle Ingredients Americas Llc filed Critical Codexis Inc
Publication of SG11202100470WA publication Critical patent/SG11202100470WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/252—Naphthacene radicals, e.g. daunomycins, adriamycins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
SG11202100470WA 2018-07-30 2019-07-17 Engineered glycosyltransferases and steviol glycoside glucosylation methods SG11202100470WA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862712199P 2018-07-30 2018-07-30
US201862712327P 2018-07-31 2018-07-31
PCT/US2019/042151 WO2020028039A1 (en) 2018-07-30 2019-07-17 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Publications (1)

Publication Number Publication Date
SG11202100470WA true SG11202100470WA (en) 2021-02-25

Family

ID=69178045

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202100470WA SG11202100470WA (en) 2018-07-30 2019-07-17 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Country Status (13)

Country Link
US (2) US11760981B2 (ja)
EP (1) EP3830106A4 (ja)
JP (4) JP2021532757A (ja)
KR (1) KR20210040408A (ja)
CN (1) CN112805295A (ja)
AU (1) AU2019314181B2 (ja)
BR (1) BR112021001661A8 (ja)
CA (1) CA3108268A1 (ja)
IL (1) IL280302A (ja)
MX (1) MX2021001260A (ja)
SG (1) SG11202100470WA (ja)
TW (1) TW202019951A (ja)
WO (1) WO2020028039A1 (ja)

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CN111621482B (zh) * 2020-06-30 2022-04-29 浙江工业大学 一种草铵膦脱氢酶突变体、基因工程菌及一锅法多酶同步定向进化方法
US20230304055A1 (en) 2020-07-03 2023-09-28 C-Lecta Gmbh One-pot cell-free glycosylation process
CN112760302B (zh) * 2020-12-23 2022-08-26 中化健康产业发展有限公司 一种能够催化莱鲍迪苷A生成莱鲍迪苷D的糖基转移酶StUGT
JP2024520118A (ja) * 2021-06-01 2024-05-21 エーバイオケム バイオテクノロジー（グループ）カンパニー，リミティド 糖転移酵素及びその使用
CN115418358B (zh) * 2021-06-01 2024-01-30 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
CN115449514B (zh) * 2021-06-08 2023-09-29 弈柯莱生物科技(上海)股份有限公司 一种β-1,2-糖基转移酶及其应用
CN113462670B (zh) * 2021-08-23 2023-03-24 兴化格林生物制品有限公司 一种糖基转移酶突变体及其催化合成莱鲍迪苷m的方法
CN115725528B (zh) * 2021-08-30 2024-01-12 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
CN116555210A (zh) * 2022-01-30 2023-08-08 弈柯莱生物科技(上海)股份有限公司 糖基转移酶及其在制备莱鲍迪苷e中的应用
WO2023226978A1 (zh) * 2022-05-23 2023-11-30 苏州引航生物科技有限公司 微生物来源的蔗糖合酶及其应用
WO2024050533A2 (en) * 2022-09-02 2024-03-07 Arzeda Corp. Compositions and methods for producing rebaudioside m
WO2024121721A1 (en) 2022-12-05 2024-06-13 Tate & Lyle Solutions Usa Llc Methods for obtaining steviol glycosides

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Also Published As

Publication number Publication date
AU2019314181A1 (en) 2021-02-04
JP2022132485A (ja) 2022-09-08
TW202019951A (zh) 2020-06-01
EP3830106A4 (en) 2022-07-06
MX2021001260A (es) 2021-04-12
CN112805295A (zh) 2021-05-14
US11760981B2 (en) 2023-09-19
US20240010999A1 (en) 2024-01-11
AU2019314181B2 (en) 2024-10-17
BR112021001661A8 (pt) 2022-08-09
CA3108268A1 (en) 2020-02-06
EP3830106A1 (en) 2021-06-09
JP2024114921A (ja) 2024-08-23
WO2020028039A1 (en) 2020-02-06
BR112021001661A2 (pt) 2021-05-04
IL280302A (en) 2021-03-01
KR20210040408A (ko) 2021-04-13
US20200032227A1 (en) 2020-01-30
JP2023039908A (ja) 2023-03-22
JP2021532757A (ja) 2021-12-02

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