SG11201911985UA - Dna sequencing using hydrogel beads - Google Patents

Dna sequencing using hydrogel beads

Info

Publication number
SG11201911985UA
SG11201911985UA SG11201911985UA SG11201911985UA SG11201911985UA SG 11201911985U A SG11201911985U A SG 11201911985UA SG 11201911985U A SG11201911985U A SG 11201911985UA SG 11201911985U A SG11201911985U A SG 11201911985UA SG 11201911985U A SG11201911985U A SG 11201911985UA
Authority
SG
Singapore
Prior art keywords
dna sequencing
hydrogel beads
hydrogel
beads
sequencing
Prior art date
Application number
SG11201911985UA
Other languages
English (en)
Inventor
Yir-Shyuan Wu
Filiz Gorpe-Yasar
Tarun Kumar Khurana
Victoria Popic
Erich B Jaeger
Mostafa Ronaghi
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=65576697&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG11201911985U(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG11201911985UA publication Critical patent/SG11201911985UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Dispersion Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
SG11201911985UA 2018-02-13 2019-02-11 Dna sequencing using hydrogel beads SG11201911985UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862630175P 2018-02-13 2018-02-13
PCT/US2019/017521 WO2019160820A1 (fr) 2018-02-13 2019-02-11 Séquençage d'adn à l'aide de billes d'hydrogel

Publications (1)

Publication Number Publication Date
SG11201911985UA true SG11201911985UA (en) 2020-01-30

Family

ID=65576697

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201911985UA SG11201911985UA (en) 2018-02-13 2019-02-11 Dna sequencing using hydrogel beads

Country Status (11)

Country Link
US (2) US11180752B2 (fr)
EP (3) EP4253562A3 (fr)
JP (3) JP7027459B2 (fr)
KR (2) KR102437660B1 (fr)
CN (1) CN111094589A (fr)
AU (2) AU2019220559B2 (fr)
CA (2) CA3067140C (fr)
DK (1) DK3752634T3 (fr)
ES (1) ES2927304T3 (fr)
SG (1) SG11201911985UA (fr)
WO (1) WO2019160820A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2964526B1 (fr) 2014-03-27 2017-09-06 SZ DJI Technology Co., Ltd. Décollage assisté
KR102656644B1 (ko) 2015-02-09 2024-04-09 슬링샷 바이오사이언시즈 인코포레이티드 튜닝가능한 광 특성을 갖는 하이드로겔 입자 및 이를 사용하기 위한 방법
CA3067140C (fr) 2018-02-13 2023-03-21 Illumina, Inc. Sequencage d'adn a l'aide de billes d'hydrogel
KR20240052875A (ko) 2018-04-20 2024-04-23 일루미나, 인코포레이티드 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도
AU2019364545A1 (en) 2018-10-26 2021-04-29 Illumina, Inc. Modulating polymer beads for DNA processing
TW202045709A (zh) 2019-01-29 2020-12-16 美商伊路米納有限公司 流體槽
TW202043486A (zh) 2019-01-29 2020-12-01 美商伊路米納有限公司 定序套組
CN113646441A (zh) * 2019-12-11 2021-11-12 因美纳有限公司 在流通池中的固定
WO2021116371A1 (fr) * 2019-12-12 2021-06-17 Keygene N.V. Manipulation d'acide nucléique semi-solide
JP2023511132A (ja) 2020-01-24 2023-03-16 スリングショット バイオサイエンシーズ， インコーポレイテッド 細胞様較正粒子のための組成物および方法
MX2023007842A (es) 2021-01-08 2023-09-19 Cellanome Inc Dispositivos y metodos para analizar muestras biologicas.
CN114874353B (zh) * 2022-05-09 2023-01-17 常州大学 基于壳寡糖的非病毒基因药物载体及其制备方法
US20230383332A1 (en) * 2022-05-31 2023-11-30 Bio-Rad Laboratories, Inc. Methods and hydrogel compositions for partitioning biological samples
WO2023235885A1 (fr) * 2022-06-02 2023-12-07 Slingshot Biosciences, Inc. Mimétique de cellule apoptotique

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323293C (fr) 1987-12-11 1993-10-19 Keith C. Backman Essai utilisant la reorganisation d'une sonde a l'acide nucleique dependant d'une matrice
CA1341584C (fr) 1988-04-06 2008-11-18 Bruce Wallace Methode d'amplification at de detection de sequences d'acides nucleiques
WO1989009835A1 (fr) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Procede d'amplification a base de ligase
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2801051B2 (ja) 1988-06-24 1998-09-21 アムジエン・インコーポレーテツド 核酸塩基配列を検出するための方法及び試薬
WO1990001069A1 (fr) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Procede d'amplification et de detection de sequences d'acide nucleique
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
CA2044616A1 (fr) 1989-10-26 1991-04-27 Roger Y. Tsien Sequencage de l'adn
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
AU635105B2 (en) 1990-01-26 1993-03-11 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JP3175110B2 (ja) 1994-02-07 2001-06-11 オーキッド・バイオサイエンシーズ・インコーポレイテッド リガーゼ／ポリメラーゼ媒体された単一ヌクレオチド多型のジェネティックビットアナリシスおよび遺伝子解析におけるその使用
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5958451A (en) 1996-09-03 1999-09-28 Yung Shin Pharm Ind. Co., Ltd. Process for producing porous, controlled-release capsules and encapsulated composition
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
ES2563643T3 (es) 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20050191698A1 (en) 1999-04-20 2005-09-01 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20020006617A1 (en) 2000-02-07 2002-01-17 Jian-Bing Fan Nucleic acid detection methods using universal priming
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
CA2415897A1 (fr) 2000-07-07 2002-01-17 Susan H. Hardin Determination de sequence en temps reel
EP1354064A2 (fr) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2003205241A1 (en) 2002-01-22 2003-09-02 The Penn State Research Foundation Drug candidate screening systems based on micropatterned hydrogels and microfluidic systems
EP1488006B1 (fr) * 2002-03-20 2008-05-28 InnovativeBio.Biz Microcapsules encapsulant un melange de reaction d'amplification de l'acide nucleique et utilisation de celles-ci en tant que compartiments de reaction pour des reactions paralleles
DK3363809T3 (da) 2002-08-23 2020-05-04 Illumina Cambridge Ltd Modificerede nukleotider til polynukleotidsekvensering
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
WO2005003304A2 (fr) 2003-06-20 2005-01-13 Illumina, Inc. Methodes et compositions utiles pour l'amplification et le genotypage du genome tout entier
CN101914620B (zh) 2004-09-17 2014-02-12 加利福尼亚太平洋生命科学公司 核酸测序的方法
WO2006125458A1 (fr) * 2005-05-27 2006-11-30 ETH Zürich Sequençage parallele d'acides nucleiques transformees dans des cellules encapsulees
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US8241573B2 (en) 2006-03-31 2012-08-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2008051530A2 (fr) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Enzymes polymèrases et réactifs pour le séquençage amélioré d'acides nucléiques
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
ES2923759T3 (es) 2006-12-14 2022-09-30 Life Technologies Corp Aparato para medir analitos utilizando matrices de FET
US9029085B2 (en) * 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US9345809B2 (en) 2007-11-28 2016-05-24 Fziomed, Inc. Carboxymethylcellulose polyethylene glycol compositions for medical uses
WO2010003132A1 (fr) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Utilisation de populations de billes dans la fabrication de matrices sur des surfaces
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2012058096A1 (fr) 2010-10-27 2012-05-03 Illumina, Inc. Microdispositifs et cartouches de biocapteurs pour analyse biologique ou chimique et systèmes et procédés associés
US9429567B2 (en) 2011-06-22 2016-08-30 Toshihiro Nakajima Method for screening substances having weight-regulating action
KR101336552B1 (ko) * 2011-08-26 2013-12-03 포항공과대학교 산학협력단 전립선암 특이적 ｓｉＲＮＡ 전달체
WO2013181265A1 (fr) 2012-05-29 2013-12-05 The Johns Hopkins University Mutants de transposase eucaryotes et compositions d'extrémité de transposon pour modifier des acides nucléiques et procédés de production et d'utilisation de la génération de bibliothèques de séquençage
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10105702B2 (en) * 2013-03-15 2018-10-23 Lariat Biosciences, Inc. Microfluidic methods for manipulating DNA
CN105339503B (zh) 2013-05-23 2020-04-10 斯坦福大学托管董事会 用于个人表观基因组学的至天然染色质的转座
CA2959978A1 (fr) 2013-09-24 2015-04-02 The Regents Of The University Of California Capteurs et systemes de capteurs encapsules pour biodosages et diagnostics et leurs procedes de fabrication et d'utilisation
WO2015088299A1 (fr) 2013-12-09 2015-06-18 Vilnius University Procédé pour la production de particules et de gouttelettes à base de biopolymère dans un système microfluidique
WO2015085899A1 (fr) 2013-12-09 2015-06-18 The University Of Hong Kong Émulsions entièrement aqueuses stabilisées et leurs procédés de réalisation et d'utilisation
WO2015147147A1 (fr) 2014-03-26 2015-10-01 国立大学法人北海道大学 Procédé de fixation haute vitesse d'échantillon biologique à l'aide d'hydrogel et son dispositif
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
KR102379805B1 (ko) 2014-07-02 2022-03-30 라이프 테크놀로지스 코포레이션 센서 기질에 로딩하기 위한 방법
EP3725893A1 (fr) 2015-02-10 2020-10-21 Illumina, Inc. Compositions pour l'analyse de composants cellulaires
US9968558B2 (en) 2015-03-20 2018-05-15 Saint Louis University Fabrication of hydrogel microsphere delivery vehicles through electrospraying and timed gelation
GB201512725D0 (en) 2015-07-20 2015-08-26 Life Technologies As Polymeric particles
WO2017040024A1 (fr) 2015-09-03 2017-03-09 The University Of Massachusetts Nanocapsules polymères stabilisées, dispersions comprenant les nanocapsules et procédés de traitement de biofilms bactériens
WO2017075265A1 (fr) 2015-10-28 2017-05-04 The Broad Institute, Inc. Analyse multiplexée de constituants unicellulaires
AU2016365724B2 (en) 2015-12-08 2023-10-12 Berkeley Lights, Inc. Microfluidic devices and kits and methods for use thereof
EP3525933B1 (fr) 2016-10-11 2024-07-03 The Regents of the University of California Systèmes et procédés pour encapsuler et conserver une matière organique en vue d'une analyse
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
EP3592847B1 (fr) * 2017-04-26 2024-06-05 10X Genomics, Inc. Variants de transcriptase inverse du virus de la leucémie murine de moloney (mmlv)
CN107281497B (zh) * 2017-07-10 2020-08-04 上海交通大学 基于dna水凝胶的功能性核酸保护性载体及其制备方法、应用
US20220411859A1 (en) 2017-08-01 2022-12-29 Illumina, Inc. Hydrogel beads for nucleotide sequencing
EP4289967A3 (fr) 2017-08-01 2024-03-20 Illumina, Inc. Indexation spatiale de matériel génétique et préparation de pharmacothèque à l'aide de billes d'hydrogel et de cellules d'écoulement
CA3067140C (fr) 2018-02-13 2023-03-21 Illumina, Inc. Sequencage d'adn a l'aide de billes d'hydrogel

Also Published As

Publication number Publication date
AU2019220559B2 (en) 2021-10-28
AU2019220559A1 (en) 2020-01-02
JP7457044B2 (ja) 2024-03-27
KR20220123327A (ko) 2022-09-06
JP7027459B2 (ja) 2022-03-01
JP2020532948A (ja) 2020-11-19
KR102437660B1 (ko) 2022-08-29
CA3186025A1 (fr) 2019-08-22
EP4253562A3 (fr) 2023-10-25
ES2927304T3 (es) 2022-11-04
CN111094589A (zh) 2020-05-01
EP3752634B8 (fr) 2023-10-11
KR20200024170A (ko) 2020-03-06
US20220106588A1 (en) 2022-04-07
CA3067140C (fr) 2023-03-21
CA3067140A1 (fr) 2019-08-22
WO2019160820A1 (fr) 2019-08-22
EP3752634B1 (fr) 2022-08-31
EP3752634A1 (fr) 2020-12-23
US20190249171A1 (en) 2019-08-15
US11180752B2 (en) 2021-11-23
EP4253562A2 (fr) 2023-10-04
DK3752634T3 (da) 2022-10-24
EP4083225A1 (fr) 2022-11-02
JP2022069461A (ja) 2022-05-11
AU2022200446A1 (en) 2022-02-17
JP2024032734A (ja) 2024-03-12

Similar Documents

Publication Publication Date Title
SG11201911985UA (en) Dna sequencing using hydrogel beads
SG11201911871TA (en) Hydrogel beads for nucleotide sequencing
EP3408219A4 (fr) Appareil de séquençage d'adn massivement parallèle
GB201704402D0 (en) Single cell DNA sequencing
HUE059673T2 (hu) Nukleinsav-szekvenálás affinitási reagensek felhasználásával
CA196154S (en) Sequencing instrument
EP3472359A4 (fr) Séquençage d'acide nucléique
EP3371194A4 (fr) Procédés et réactifs de séquençage de protéines
EP3259372A4 (fr) Séquençage de l'adn à l'aide d'un déplacement de brin régulé
IL281804A (en) Protein purification methods
IL281539A (en) Modulating polymer beads for DNA processing
IL273426A (en) Systems and methods for nucleic acid sequencing
GB2589159B (en) Nucleic acid sequencing apparatus
SG11202003885UA (en) Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
EP3508587A4 (fr) Procédé de séquençage d'adn
GB201907242D0 (en) Dna methods etc ii
IL280628A (en) Methods for protein resequencing
EP3277845A4 (fr) Billes destinées au séquençage d'acides nucléiques
SG11202012758WA (en) Primer oligonucleotide for sequencing
IL267445A (en) An array that includes a genetic tiling primer and a non tiling entity
IL285396A (en) Biological flooring
EP3802821A4 (fr) Procédé de séquençage direct d'acides nucléiques
PL3663407T3 (pl) Sposób sekwencjonowania kwasów nukleinowych
SG11202012753SA (en) Sequencing kits
GB201704404D0 (en) Beads for single cell sequencing