SG11201908541SA - Bone marrow microfluidic devices and methods for preparing and using the same - Google Patents

Bone marrow microfluidic devices and methods for preparing and using the same

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Publication number
SG11201908541SA
SG11201908541SA SG11201908541SA SG11201908541SA SG 11201908541S A SG11201908541S A SG 11201908541SA SG 11201908541S A SG11201908541S A SG 11201908541SA SG 11201908541S A SG11201908541S A SG 11201908541SA
Authority
SG
Singapore
Prior art keywords
massachusetts
international
bone marrow
street
microfluidic devices
Prior art date
Application number
Inventor
David Chou
Liliana Leijten
Arianna Rech
Richard Novak
Donald Ingber
Yuka Milton
Viktoras Frismantas
Oren Levy
Original Assignee
Harvard College
Massachusetts Gen Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Massachusetts Gen Hospital filed Critical Harvard College
Publication of SG11201908541SA publication Critical patent/SG11201908541SA/en

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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0669—Bone marrow stromal cells; Whole bone marrow
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
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    • C12N2501/40—Regulators of development
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    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 September 2018 (27.09.2018) WIP0 I PCT ~~ll~~~~~~~~ 011101010VIIIOH olo olomiloonmll oo mom ow (10) International Publication Number WO 2018/176043 Al (51) International Patent Classification: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) BOlD 59/36 (2006.01) C12Q 1/02 BO1J 19/00 (2006.01) C12Q 1/68 C12M 1/00 (2006.01) C12Q 1/70 C12N 5/00 (2006.01) (2006.01) (2018.01) (2006.01) (21) International Application Number: PCT/US2018/024377 (22) International Filing Date: 26 March 2018 (26.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/476,205 24 March 2017 (24.03.2017) US (71) Applicants: PRESIDENT AND FELLOWS OF HAR- VARD COLLEGE [US/US]; 17 Quincy Street, Cam- bridge, Massachusetts 02138 (US). THE GENERAL HOSPITAL CORPORATION [US/US]; 55 Fruit Street, Boston, Massachusetts 02114 (US). = (72) Inventors: CHOU, David, Benson; 150 Staniford Street, Unit 429, Boston, Massachusetts 02114 (US). LEIJTEN, Liliana, S. Teixeira Moreira; Haspengouw 55R, Maas- tricht, 6227 RM Limburg (NL). RECH, Arianna; 525 New Ashby Road, Loughborough Leicestershire LE11 4EX (GB). NOVAK, Richard; 42 Rockview Street, Apt. 4, Ja- maica Plain, Massachusetts 02130 (US). INGBER, Don- ald, E.; 162 W. Canton St., Boston, Massachusetts 02118 (US). MILTON, Yuka; 223 Charles Street, Apt. 1, Cam- bridge, Massachusetts 02141 (US). FRISMANTAS, Vik- toras; 20 Fisher Ave., Apt. 1, Roxburg Crossing, Massa- chusetts 02120 (US). LEVY, Oren; 1386 Beacon St., Apt. 15, Brookline, Massachusetts 02446 (US). (74) Agent: RESNICK, David, S. et al.; Nixon Peabody LLP, 70 W. Madison St., Suite 3500, Chicago, Illinois 60602-4224 (US). 1-1 71' O N 1-1 00 1-1 O N C (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: BONE MARROW MICROFLUIDIC DEVICES AND METHODS FOR PREPARING AND USING THE SAME (57) : The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.
SG11201908541S 2017-03-24 2018-03-26 Bone marrow microfluidic devices and methods for preparing and using the same SG11201908541SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762476205P 2017-03-24 2017-03-24
PCT/US2018/024377 WO2018176043A1 (en) 2017-03-24 2018-03-26 Bone marrow microfluidic devices and methods for preparing and using the same

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US (2) US11845920B2 (en)
EP (1) EP3600627A4 (en)
AU (1) AU2018239659B2 (en)
CA (1) CA3056682A1 (en)
GB (2) GB2608510B (en)
SG (1) SG11201908541SA (en)
WO (1) WO2018176043A1 (en)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030087290A1 (en) * 2002-01-14 2003-05-08 Tarlov Michael J. Bio-affinity porous matrix in microfluidic channels
AU2009270821B2 (en) * 2008-07-16 2015-05-14 Children's Medical Center Corporation Organ mimic device with microchannels and methods of use and manufacturing thereof
KR101264478B1 (en) * 2010-05-24 2013-05-14 삼성전기주식회사 cell chip
JP6662566B2 (en) * 2011-06-02 2020-03-11 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Methods and uses for ex vivo tissue culture systems
WO2013086502A1 (en) * 2011-12-09 2013-06-13 President And Fellows Of Harvard College Organ chips and uses thereof
US9725687B2 (en) 2011-12-09 2017-08-08 President And Fellows Of Harvard College Integrated human organ-on-chip microphysiological systems
AU2014386207B2 (en) 2013-12-20 2019-08-22 President And Fellows Of Harvard College Organomimetic devices and methods of use and manufacturing thereof
WO2016010861A1 (en) 2014-07-14 2016-01-21 President And Fellows Of Harvard College Systems and methods for improved performance of fluidic and microfluidic systems
US20160097033A1 (en) * 2014-10-06 2016-04-07 University Of Washington Blood-cell producing bio-microreactor

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