SG11201811309YA - Detecting microscopic objects in fluids - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property :::` , Organization _, 1 MEM 0 11101 HOE 010 1111 1 0 01 OH 010111111101100110111011110111111 International Bureau (10) International Publication Number (43) International Publication Date ......°' WO 2017/220860 Al 28 December 2017 (28.12.2017) WIP0 I PCT (51) International Patent Classification: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, GO1N 15/02 (2006.01) GO1N 21/85 (2006.01) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, GO1N 15/10 (2006.01) GO3H 1/00 (2006.01) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, GO6T 7/10 (2017.01) OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (21) International Application Number: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, PCT/FI2017/050463 TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (22) International Filing Date: (84) Designated States (unless otherwise indicated, for every 21 June 2017 (21.06.2017) kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (25) Filing Language: English UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (26) Publication Language: English TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (30) Priority Data: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, 16175687.9 22 June 2016 (22.06.2016) EP TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (71) Applicant: UWATER OY [MI]; Korkeakoulunkatu 10, 33720 Tampere (FI). Declarations under Rule 4.17: (72) Inventors: KESTI, Tero; Kotkansiivenkatu 2 A 6, 33720 — as to applicant's entitlement to apply for and be granted a Tampere (FI). HAMALAINEN, Esa; Ketunkuja 5, 37100 patent (Rule 4.17(ii)) Nokia (FI). — of inventorship (Rule 4.17(iv)) (74) Agent: PAPULA OY; P.O. Box 981, 00101 Helsinki (FI). Published: — — with international search report (Art. 21(3)) (81) Designated States (unless otherwise indicated, for every = _ kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, = CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, = (54) Title: DETECTING MICROSCOPIC OBJECTS IN FLUIDS = = (57) : A method (10) comprises: obtaining (11) prepared image da- - to captured by an image sensor receiving light across a sample propagated = = = = Obtaining prepared image data comprising a prepared hologram pattern, originating from a hologram digital image frame volume, containing a fluid possibly comprising microscopic objects of for- eign origin, while illuminating the sample volume by coherent light, the pre- pared image data comprising, for a microscopic object, a prepared hologram = - 11 with spatially alternating intensity formed by interference pattern prepared = = = Providing filtered image data by filtering the prepared image data by edge enhancing filter, the filtered image data comprising a filtered hologram pattern fringes; providing (12) filtered image data, comprising automatically filtering the prepared image data by an edge enhancing filter, the filtered image data comprising, for a prepared hologram pattern, a filtered hologram pattern; and = 12 automatically detecting (13), on the basis of the filtered hologram pattern, the = Automatically detecting, on the basis of the filtered hologram pattern, the presence of the microscopic object associated with the filtered hologram pattern presence of the microscopic object associated with the filtered hologram pat- tern in the sample volume of the fluid. _ 1 13 / 220860 Al Automatically determining a shape parameter of the detected microscopic object on the basis of the shape of the associated filtered hologram pattern 14 Automatically identifying, for the filtered hologram pattern, the associated prepared hologram pattern and automatically determining a size parameter of the associated microscopic object on the basis of the maximum intensity of the associated prepared hologram pattern N 1-1 14 OPTIONAL, 10 0 N Fig. 1 C