SG11201908847TA - Apparatuses, systems and methods for imaging flow cytometry - Google Patents

Apparatuses, systems and methods for imaging flow cytometry

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Publication number
SG11201908847TA
SG11201908847TA SG11201908847TA SG11201908847TA SG 11201908847T A SG11201908847T A SG 11201908847TA SG 11201908847T A SG11201908847T A SG 11201908847TA SG 11201908847T A SG11201908847T A SG 11201908847TA
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SG
Singapore
Prior art keywords
international
carlsbad
california
pct
systems
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Gregory Kaduchak
Michael Ward
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Life Technologies Corp
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Publication of SG11201908847TA publication Critical patent/SG11201908847TA/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N15/1436—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
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    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
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    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
    • G01N15/0227—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging using imaging, e.g. a projected image of suspension; using holography
    • G—PHYSICS
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    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
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    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/53—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke
    • G01N21/532—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke with measurement of scattering and transmission
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/082—Active control of flow resistance, e.g. flow controllers
    • G01N15/149—
    • G—PHYSICS
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    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0003—Determining electric mobility, velocity profile, average speed or velocity of a plurality of particles
    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0007—Investigating dispersion of gas
    • G01N2015/0011—Investigating dispersion of gas in liquids, e.g. bubbles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/03—Electro-optical investigation of a plurality of particles, the analyser being characterised by the optical arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G01N2015/1028—
    • G—PHYSICS
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    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/1415—Control of particle position
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/142—Acoustic or ultrasonic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
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    • G01N2015/1422—Electrical focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N15/10—Investigating individual particles
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    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/1438—Using two lasers in succession
    • G—PHYSICS
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    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/144—Imaging characterised by its optical setup
    • G01N2015/1445—Three-dimensional imaging, imaging in different image planes, e.g. under different angles or at different depths, e.g. by a relative motion of sample and detector, for instance by tomography
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
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    • G01N2015/1452—Adjustment of focus; Alignment

Abstract

V FIG. 2 12.906 7-201 Detector 206- \ Illumination Source 202 Th. Illumination Source Image Detector (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 October 2018 (04.10.2018) W I PO I PCT 0111111010 0111 °nolo 0111011101110111 1101111111 (10) International Publication Number WO 2018/183939 Al (51) International Patent Classification: GO1N 15/14 (2006.01) GO1N 15/10 (2006.01) BOIL 3/00 (2006.01) (21) International Application Number: PCT/US2018/025538 (22) International Filing Date: 30 March 2018 (30.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/479,734 31 March 2017 (31.03.2017) US (71) Applicant: LIFE TECHNOLOGIES CORPORATION [US/US]; 5823 Newton Drive, Carlsbad, California 92008 (US). (72) Inventors: KADUCHAK, Gregory; 5823 Newton Drive, Carlsbad, California 92008 (US). WARD, Michael; 5823 Newton Drive, Carlsbad, California 92008 (US). (74) Agent: YEE, Steven; 5823 Newton Drive, Carlsbad, Cali- fornia 92009 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: APPARATUSES, SYSTEMS AND METHODS FOR IMAGING FLOW CYTOMETRY (57) : The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cy- ..) tometry at comparatively high event rates and for gathering high resolution images of particles.
SG11201908847T 2017-03-31 2018-03-30 Apparatuses, systems and methods for imaging flow cytometry SG11201908847TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762479734P 2017-03-31 2017-03-31
PCT/US2018/025538 WO2018183939A1 (en) 2017-03-31 2018-03-30 Apparatuses, systems and methods for imaging flow cytometry

Publications (1)

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SG11201908847TA true SG11201908847TA (en) 2019-10-30

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Country Status (7)

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US (8) US10386290B2 (en)
EP (1) EP3602003A1 (en)
JP (2) JP7187474B2 (en)
KR (1) KR102585276B1 (en)
CN (2) CN110573858B (en)
SG (1) SG11201908847TA (en)
WO (1) WO2018183939A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017108129A1 (en) * 2015-12-23 2017-06-29 Siemens Healthcare Gmbh Flow cell for analyzing particles in a liquid to be examined
CA3106121A1 (en) * 2018-07-10 2020-01-16 Gerrit Jan Van Den Engh System, apparatus and method for off-axis illumination in flow cytometry
CN109470672A (en) * 2018-12-06 2019-03-15 大连海事大学 The active apparatus and method of more single microalgae cells of light intensity excitation-detection
WO2020241226A1 (en) * 2019-05-30 2020-12-03 ソニーセミコンダクタソリューションズ株式会社 Optical measurement device and optical measurement system
JP6745559B1 (en) * 2020-03-24 2020-08-26 株式会社Ｃｙｂｏ Imaging flow cytometer, sorting method, and calibration method
KR102559634B1 (en) * 2020-08-26 2023-07-26 한국전자통신연구원 Apparatus and method for pcr diagnosis based on multiwavelength light source
WO2022107557A1 (en) * 2020-11-18 2022-05-27 ソニーグループ株式会社 Biological sample analysis system, information processing device, information processing method, and biological sample analysis method
US11788949B2 (en) 2020-12-14 2023-10-17 Life Technologies Corporation Systems and methods for improved imaging and fluorescence in flow cytometry and other applications
US11639895B2 (en) 2021-03-09 2023-05-02 Globalfoundries U.S. Inc. Device including optofluidic sensor with integrated photodiode
WO2023276298A1 (en) * 2021-07-01 2023-01-05 ソニーグループ株式会社 Biological sample analysis system, information processing device, information processing method, and biological sample analysis method
WO2023220478A1 (en) * 2022-05-13 2023-11-16 Bio-Rad Laboratories, Inc. Method and system for detection of particles focused asymmetrically

Family Cites Families (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS499288B1 (en) * 1968-12-19 1974-03-04
US4171159A (en) 1977-07-14 1979-10-16 White Matthew B Optical homodyne microscope
US4195221A (en) 1978-07-03 1980-03-25 The United States Of America As Represented By The Secretary Of The Navy Scanning focused local oscillator optical heterodyne imaging system
US4193088A (en) 1978-08-02 1980-03-11 The United States Of America As Represented By The Secretary Of The Navy Optical heterodyne system for imaging in a dynamic diffusive medium
FR2477184A1 (en) * 1980-02-28 1981-09-04 Onera (Off Nat Aerospatiale) PROCESS FOR INCREASING THE LIFETIME OF A PART IN A REFRACTORY MATERIAL WITH PARALLEL FIBERS OF METAL DARBIDE DERBATED IN A METALLIC MATRIX
JPS59184841A (en) * 1983-04-05 1984-10-20 ベクトン・デイツキンソン・アンド・カンパニ− Method and device for discriminating sub-class of leukocyte in sample
US4786165A (en) 1986-07-10 1988-11-22 Toa Medical Electronics Co., Ltd. Flow cytometry and apparatus therefor
JPH0734012B2 (en) 1991-02-27 1995-04-12 東亜医用電子株式会社 Flow image cytometer
JP3084296B2 (en) 1991-02-27 2000-09-04 シスメックス株式会社 Flow image cytometer
JP3084295B2 (en) 1991-02-27 2000-09-04 シスメックス株式会社 Flow image cytometer
US5412466A (en) 1991-07-26 1995-05-02 Toa Medical Electronics Co., Ltd. Apparatus for forming flattened sample flow for analyzing particles
JP3212647B2 (en) 1991-10-24 2001-09-25 シスメックス株式会社 Imaging flow cytometer
JP3102935B2 (en) 1991-11-20 2000-10-23 シスメックス株式会社 Imaging flow cytometer
JP3102938B2 (en) 1991-12-30 2000-10-23 シスメックス株式会社 Particle image analyzer
JP3187129B2 (en) 1992-04-01 2001-07-11 シスメックス株式会社 Particle analyzer
JP3145486B2 (en) 1992-06-12 2001-03-12 シスメックス株式会社 Imaging flow cytometer
JP3145487B2 (en) 1992-06-12 2001-03-12 シスメックス株式会社 Particle analyzer
JP3215175B2 (en) 1992-08-10 2001-10-02 シスメックス株式会社 Particle analyzer
JPH06186156A (en) 1992-10-21 1994-07-08 Toa Medical Electronics Co Ltd Particle analyzer
JP3052665B2 (en) * 1993-01-26 2000-06-19 株式会社日立製作所 Flow cell device
US5418371A (en) 1993-02-01 1995-05-23 Aslund; Nils R. D. Apparatus for quantitative imaging of multiple fluorophores using dual detectors
JPH07120375A (en) 1993-10-21 1995-05-12 Hitachi Ltd Method and apparatus for flow-type particle image analysis
JPH07270301A (en) * 1994-03-31 1995-10-20 Hitachi Ltd Flow type particle image analysis method and apparatus
DE69533469T2 (en) 1994-12-26 2005-09-22 Sysmex Corp. flow cytometer
US5694216A (en) 1996-04-25 1997-12-02 University Of Central Florida Scanning heterodyne acousto-optical interferometers
US5908599A (en) * 1996-07-30 1999-06-01 Bayer Corporation Heated reaction chamber in a unified fluid circuit of a hematology diagnostic instrument
US5872627A (en) * 1996-07-30 1999-02-16 Bayer Corporation Method and apparatus for detecting scattered light in an analytical instrument
US6831781B2 (en) 1998-02-26 2004-12-14 The General Hospital Corporation Confocal microscopy with multi-spectral encoding and system and apparatus for spectroscopically encoded confocal microscopy
US6151127A (en) 1998-05-28 2000-11-21 The General Hospital Corporation Confocal microscopy
JP3871456B2 (en) 1998-12-10 2007-01-24 シスメックス株式会社 Particle image analyzer
US20010006416A1 (en) 1999-01-11 2001-07-05 Johnson Paul E. Ribbon flow cytometry apparatus and methods
US6256096B1 (en) 1999-01-11 2001-07-03 Softray Flow cytometry apparatus and method
US6249341B1 (en) 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US7450229B2 (en) 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US6671044B2 (en) 1999-01-25 2003-12-30 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells in broad flat flow
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US7057732B2 (en) 1999-01-25 2006-06-06 Amnis Corporation Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis
US6309886B1 (en) 1999-06-04 2001-10-30 The Regents Of The University Of California High throughput analysis of samples in flowing liquid
US6813017B1 (en) 1999-10-20 2004-11-02 Becton, Dickinson And Company Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
US6934408B2 (en) 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
WO2002017219A1 (en) 2000-08-25 2002-02-28 Amnis Corporation Measuring the velocity of small moving objects such as cells
US6583865B2 (en) 2000-08-25 2003-06-24 Amnis Corporation Alternative detector configuration and mode of operation of a time delay integration particle analyzer
US6875973B2 (en) 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
US6608680B2 (en) 2000-08-25 2003-08-19 Amnis Corporation TDI imaging system for kinetic studies
US6778263B2 (en) 2000-08-25 2004-08-17 Amnis Corporation Methods of calibrating an imaging system using calibration beads
AU2002213157A1 (en) 2000-10-12 2002-04-22 Amnis Corporation System and method for high numeric aperture imaging systems
US6563583B2 (en) 2000-10-12 2003-05-13 Amnis Corporation Multipass cavity for illumination and excitation of moving objects
US6872291B2 (en) * 2001-01-09 2005-03-29 Ppg Industries Ohio, Inc. Method and device for detecting and controlling the level of biological contaminants in a coating process
US6467350B1 (en) 2001-03-15 2002-10-22 The Regents Of The University Of California Cylindrical acoustic levitator/concentrator
WO2002086416A2 (en) 2001-04-25 2002-10-31 Amnis Corporation Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging
US6618140B2 (en) 2001-06-18 2003-09-09 Amnis Corporation Spectral deconvolution of fluorescent markers
WO2003009579A2 (en) 2001-07-17 2003-01-30 Amnis Corporation Computational methods for the segmentation of images of objects from background in a flow imaging instrument
US6765656B2 (en) 2001-12-18 2004-07-20 University Of Wyoming Apparatus and methods for high throughput analysis of samples in a translucent flowing liquid
EP1327865A1 (en) * 2002-01-14 2003-07-16 Abb Research Ltd. Method for thermal mass flow measurement using non-constant heating pulses
US7161665B2 (en) 2002-12-18 2007-01-09 University Of Wyoming High resolution imaging fountain flow cytometry
US7610942B2 (en) 2003-01-15 2009-11-03 Amnis Corporation Cell suspension rotating fluidic pump
DE10320956B4 (en) 2003-02-05 2005-02-17 Evotec Technologies Gmbh Examination procedure for biological cells and associated examination equipment
DE10304653B4 (en) 2003-02-05 2005-01-27 Evotec Technologies Gmbh Multi-parameter detection in a fluidic microsystem
BRPI0408857B1 (en) * 2003-03-28 2018-09-11 Inguran Llc apparatus, methods and processes for separating particles and for providing sex-separated animal sperm
JP3816072B2 (en) * 2003-10-28 2006-08-30 ローム株式会社 Optical waveguide sensor and measuring device using the same
US8103080B2 (en) 2004-03-16 2012-01-24 Amnis Corporation Method for imaging and differential analysis of cells
ATE538138T1 (en) 2004-03-16 2012-01-15 Amnis Corp IMAGING-BASED QUANTIFICATION OF MOLECULAR TRANSLOCATION
US7340957B2 (en) 2004-07-29 2008-03-11 Los Alamos National Security, Llc Ultrasonic analyte concentration and application in flow cytometry
US7074622B2 (en) 2004-11-15 2006-07-11 Eastman Kodak Company Method and system for sorting and separating particles
DE102004055662A1 (en) * 2004-11-18 2006-06-01 Evotec Technologies Gmbh Microfluidic system with channel widening
US7307721B2 (en) 2005-04-13 2007-12-11 Brightwell Technologies Particle imaging system with a varying flow rate
JP2007148981A (en) 2005-11-30 2007-06-14 Univ Waseda Particle sorting microsystem and particle sorting method
WO2007067999A2 (en) 2005-12-09 2007-06-14 Amnis Corporation Extended depth of field imaging for high speed object analysis
US7464356B2 (en) * 2005-12-15 2008-12-09 International Business Machines Corporation Method and apparatus for diffusion based cell placement migration
US7460248B2 (en) 2006-05-15 2008-12-02 Carestream Health, Inc. Tissue imaging system
US7835000B2 (en) 2006-11-03 2010-11-16 Los Alamos National Security, Llc System and method for measuring particles in a sample stream of a flow cytometer or the like
US7804594B2 (en) 2006-12-29 2010-09-28 Abbott Laboratories, Inc. Method and apparatus for rapidly counting and identifying biological particles in a flow stream
KR100871097B1 (en) 2007-01-08 2008-11-28 김태근 Optical imaging system based on coherence frequency domain reflectometry
EP2664916B1 (en) 2007-04-02 2017-02-08 Acoustic Cytometry Systems, Inc. Method for manipulating a fluid medium within a flow cell using acoustic focusing
US7837040B2 (en) 2007-04-09 2010-11-23 Los Alamos National Security, Llc Acoustic concentration of particles in fluid flow
US8083068B2 (en) 2007-04-09 2011-12-27 Los Alamos National Security, Llc Apparatus for separating particles utilizing engineered acoustic contrast capture particles
US8702945B2 (en) * 2007-05-18 2014-04-22 University Of Washington Time-varying flows for microfluidic particle separation
US8528406B2 (en) 2007-10-24 2013-09-10 Los Alamos National Security, LLP Method for non-contact particle manipulation and control of particle spacing along an axis
US8263407B2 (en) 2007-10-24 2012-09-11 Los Alamos National Security, Llc Method for non-contact particle manipulation and control of particle spacing along an axis
JP5259154B2 (en) 2007-10-24 2013-08-07 オリンパス株式会社 Scanning laser microscope
US8266951B2 (en) 2007-12-19 2012-09-18 Los Alamos National Security, Llc Particle analysis in an acoustic cytometer
JP4909288B2 (en) * 2008-01-10 2012-04-04 日本電信電話株式会社 Airborne particulate matter measurement device
US8714014B2 (en) 2008-01-16 2014-05-06 Life Technologies Corporation System and method for acoustic focusing hardware and implementations
DE102008017119A1 (en) 2008-04-02 2009-10-08 Polytec Gmbh Vibrometer and method for optical measurement of an object
US7796256B2 (en) 2008-05-05 2010-09-14 Fluid Imaging Technologies, Inc. Oil-immersion enhanced imaging flow cytometer
EP2300800A2 (en) * 2008-06-12 2011-03-30 East Carolina University Flow cytometer apparatus for three dimensional diffraction imaging and related methods
US8440952B2 (en) 2008-11-18 2013-05-14 The Regents Of The University Of California Methods for optical amplified imaging using a two-dimensional spectral brush
EP2409133A1 (en) * 2009-03-20 2012-01-25 Bio-Rad Laboratories, Inc. Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry
DE102009017151A1 (en) * 2009-04-15 2010-10-21 Eisenmann Anlagenbau Gmbh & Co. Kg Plant for dip treatment
US8610085B2 (en) 2009-08-20 2013-12-17 Bio-Rad Laboratories, Inc. High-speed cellular cross sectional imaging
US8451524B2 (en) 2009-09-29 2013-05-28 Amnis Corporation Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile
JP2011095181A (en) 2009-10-30 2011-05-12 Sysmex Corp Particle analyzer
WO2011068764A2 (en) * 2009-12-04 2011-06-09 Life Technologies Corporation Apparatuses, systems, methods, and computer readable media for acoustic flow cytometry
US9217635B2 (en) 2010-01-07 2015-12-22 Shilat Optronics Ltd. Three dimensional measurement system
WO2011105507A1 (en) 2010-02-24 2011-09-01 財団法人神奈川科学技術アカデミー Cell analyzer
US9068916B2 (en) 2010-03-15 2015-06-30 Bio-Rad Laboratories, Inc. Microassembled imaging flow cytometer
DE102010013829A1 (en) 2010-03-26 2011-09-29 Carl Zeiss Microlmaging Gmbh Microscope and method for detecting sample light
US8817115B1 (en) 2010-05-05 2014-08-26 Amnis Corporation Spatial alignment of image data from a multichannel detector using a reference image
US9274042B2 (en) * 2010-05-07 2016-03-01 Stc.Unm Spatially correlated light collection from multiple sample streams excited with a line focused light source
AU2011305983B2 (en) 2010-06-21 2014-06-12 The Regents Of The University Of California Differential interference contrast serial time encoded amplified microscopy
US20120002029A1 (en) 2010-06-30 2012-01-05 Fluid Imaging Technologies, Inc. System and method for sweeping mirror enhanced imaging flow cytometry
US8736818B2 (en) 2010-08-16 2014-05-27 Ball Aerospace & Technologies Corp. Electronically steered flash LIDAR
US8761486B2 (en) 2011-02-22 2014-06-24 Bio-Rad Laboratories, Inc. Line scan cytometry systems and methods
US9529008B2 (en) 2011-03-03 2016-12-27 Life Technologies Corporation Sampling probes, systems, apparatuses, and methods
WO2013003498A2 (en) 2011-06-27 2013-01-03 Life Technologies Corporation Acoustic cytometry methods and protocols
JP5849503B2 (en) 2011-08-01 2016-01-27 株式会社Ihi Particle observation device and manipulation device
JP6047870B2 (en) 2011-10-14 2016-12-21 ソニー株式会社 Apparatus, method and program for 3D data analysis, and fine particle analysis system
WO2013132344A2 (en) 2012-03-09 2013-09-12 University Of Manitoba Split-sensor dielectrophoretic/magnetophoretic cytometer
US8879797B2 (en) 2012-05-25 2014-11-04 Fluid Imaging Technologies, Inc. System and method for total internal reflection enhanced imaging flow cytometry
EP2864762A4 (en) * 2012-06-21 2016-01-20 Stc Unm Spatially correlated light collection from multiple sample streams excited with a line focused light source
US9201008B2 (en) 2012-06-26 2015-12-01 Universite Laval Method and system for obtaining an extended-depth-of-field volumetric image using laser scanning imaging
JP6396911B2 (en) * 2012-10-15 2018-09-26 ナノセレクト バイオメディカル， インコーポレイテッド System, apparatus and method for sorting particles
US10545041B2 (en) * 2012-10-16 2020-01-28 Xtralis Technologies, Ltd. Addressability in particle detection
EP2943778B1 (en) 2013-01-09 2020-10-28 The Regents of The University of California Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
JP2014174139A (en) * 2013-03-13 2014-09-22 Sony Corp Flow channel device, particle sorter, particle outflow method, and particle sorting method
BR112015019969B1 (en) 2013-03-15 2021-01-05 Iris International, Inc. method for obtaining particle images using a particle analysis system configured for combined hydrodynamic focusing of viscosity and geometry, and a particle analysis system that performs combined hydrodynamic focusing of viscosity and geometry for image particles in a sample of blood
BR112015021902B1 (en) 2013-03-15 2021-06-15 Iris International, Inc LIQUID FOR INTRACELLULAR PARTICLE AND ORGANELA ALIGNMENT
JP2016521362A (en) * 2013-04-12 2016-07-21 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Automatic setup for cell sorting
US9372143B2 (en) 2013-05-15 2016-06-21 Captl Llc Scanning image flow cytometer
AU2014273560B2 (en) * 2013-05-28 2018-07-19 Chemometec A/S Image forming cytometer
CN103308440A (en) 2013-05-28 2013-09-18 香港浸会大学深圳研究院 Flow type fluorescence microscopy imaging device and method
US10061111B2 (en) * 2014-01-17 2018-08-28 The Trustees Of Columbia University In The City Of New York Systems and methods for three dimensional imaging
US9726593B2 (en) 2014-02-28 2017-08-08 Life Technologies Corporation Systems, methods, and apparatuses for optical systems in flow cytometers
AU2015225730A1 (en) 2014-03-06 2016-10-27 Life Technologies Corporation Systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer
AU2015307593B2 (en) 2014-08-28 2017-04-13 Sysmex Corporation Particle imaging apparatus and particle imaging method
CN104266956A (en) * 2014-09-02 2015-01-07 上海凯度机电科技有限公司 High content image flow biological microscopic analysis method
CN104535481B (en) * 2014-12-29 2017-04-05 中国科学院长春光学精密机械与物理研究所 imaging flow cytometer
ES2824700T3 (en) 2015-02-19 2021-05-13 Becton Dickinson Co High-throughput single-cell analysis combining proteomic and genomic information
JP6030740B1 (en) 2015-12-03 2016-11-24 リオン株式会社 Particle counter
KR102599602B1 (en) 2015-12-30 2023-11-07 라이프 테크놀로지스 코포레이션 Systems and methods for providing stable fluid flow
US20180059005A1 (en) * 2016-05-26 2018-03-01 Redshift Bioanalytics, Inc. Microfluidic Methods and Apparatus for Analysis of Analyte Bearing Fluids

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