SG11201801809VA - Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method - Google Patents

Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method

Info

Publication number
SG11201801809VA
SG11201801809VA SG11201801809VA SG11201801809VA SG11201801809VA SG 11201801809V A SG11201801809V A SG 11201801809VA SG 11201801809V A SG11201801809V A SG 11201801809VA SG 11201801809V A SG11201801809V A SG 11201801809VA SG 11201801809V A SG11201801809V A SG 11201801809VA
Authority
SG
Singapore
Prior art keywords
nucleobase
molecular complex
complex used
targeted dna
dna sequence
Prior art date
Application number
SG11201801809VA
Other languages
English (en)
Inventor
Keiji Nishida
Satomi Kojima
Akihiko Kondo
Original Assignee
Univ Kobe Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kobe Nat Univ Corp filed Critical Univ Kobe Nat Univ Corp
Publication of SG11201801809VA publication Critical patent/SG11201801809VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
SG11201801809VA 2015-09-09 2016-09-08 Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method SG11201801809VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015178023 2015-09-09
PCT/JP2016/076448 WO2017043573A1 (fr) 2015-09-09 2016-09-08 Procédé de modification de séquence génomique convertissant spécifiquement une base nucléique d'une séquence d'adn ciblée, et complexe moléculaire utilisé dans ledit procédé

Publications (1)

Publication Number Publication Date
SG11201801809VA true SG11201801809VA (en) 2018-04-27

Family

ID=58239796

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201801809VA SG11201801809VA (en) 2015-09-09 2016-09-08 Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method

Country Status (10)

Country Link
US (1) US20190024098A1 (fr)
EP (1) EP3348636B1 (fr)
JP (3) JP6780860B2 (fr)
CN (1) CN108271385A (fr)
CA (1) CA2998087A1 (fr)
DK (1) DK3348636T3 (fr)
ES (1) ES2902338T3 (fr)
HK (1) HK1253540A1 (fr)
SG (1) SG11201801809VA (fr)
WO (1) WO2017043573A1 (fr)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (fr) 2011-07-22 2020-04-22 President and Fellows of Harvard College Évaluation et amélioration de la spécificité de clivage des nucléases
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
CN111500569A (zh) 2014-03-05 2020-08-07 国立大学法人神户大学 特异性转变靶向dna序列的核酸碱基的基因组序列的修饰方法、及其使用的分子复合体
WO2016022363A2 (fr) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Protéines cas9 comprenant des intéines dépendant de ligands
CN108271384B (zh) 2015-09-09 2022-04-15 国立大学法人神户大学 用于特异性转变靶向dna序列的核酸碱基的革兰氏阳性菌的基因组序列的转变方法、及其使用的分子复合体
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
US11220693B2 (en) 2015-11-27 2022-01-11 National University Corporation Kobe University Method for converting monocot plant genome sequence in which nucleic acid base in targeted DNA sequence is specifically converted, and molecular complex used therein
BR112018071376A2 (pt) * 2016-04-21 2019-04-24 National University Corporation Kobe University método para modificar um sítio alvejado de um dna de cadeia dupla, complexo enzimático modificador de ácido nucleico, e, ácido nucleico
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (fr) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Édition du gène récepteur ccr5 pour protéger contre l'infection par le vih
EP3592853A1 (fr) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression de la douleur par édition de gène
WO2018165629A1 (fr) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Éditeur de base cytosine à guanine
CA3057192A1 (fr) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Editeurs de nucleobase comprenant des proteines de liaison a l'adn programmable par acides nucleiques
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11807869B2 (en) * 2017-06-08 2023-11-07 Osaka University Method for producing DNA-edited eukaryotic cell, and kit used in the same
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
AU2018321021B2 (en) 2017-08-21 2022-10-06 Tokushima University Target sequence specific alteration technology using nucleotide target recognition
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
BR112020019301A2 (pt) 2018-03-26 2021-01-05 National University Corporation Kobe University Método para modificar um sítio alvo de um dna de fita dupla de uma célula.
CN112805379A (zh) * 2018-08-03 2021-05-14 比姆医疗股份有限公司 多效应核碱基编辑器和使用其修饰核酸靶序列的方法
WO2020191153A2 (fr) 2019-03-19 2020-09-24 The Broad Institute, Inc. Procédés et compositions pour l'édition de séquences nucléotidiques
US20220333133A1 (en) 2019-09-03 2022-10-20 Voyager Therapeutics, Inc. Vectorized editing of nucleic acids to correct overt mutations
IL296416A (en) * 2020-03-16 2022-11-01 Univ Duke Methods and compositions for improving type i-e CRISPR-based gene silencing
KR20230019843A (ko) 2020-05-08 2023-02-09 더 브로드 인스티튜트, 인코퍼레이티드 표적 이중 가닥 뉴클레오티드 서열의 두 가닥의 동시 편집을 위한 방법 및 조성물

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110104787A1 (en) * 2009-11-05 2011-05-05 President And Fellows Of Harvard College Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences
GB201122458D0 (en) * 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
US20150315576A1 (en) * 2012-11-01 2015-11-05 Massachusetts Institute Of Technology Genetic device for the controlled destruction of dna
US9737604B2 (en) * 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US20150166984A1 (en) * 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations

Also Published As

Publication number Publication date
EP3348636A1 (fr) 2018-07-18
JP2021019615A (ja) 2021-02-18
JP6780860B2 (ja) 2020-11-04
JP2022123889A (ja) 2022-08-24
US20190024098A1 (en) 2019-01-24
HK1253540A1 (zh) 2019-06-21
JPWO2017043573A1 (ja) 2018-06-21
ES2902338T3 (es) 2022-03-28
CN108271385A (zh) 2018-07-10
WO2017043573A1 (fr) 2017-03-16
EP3348636A4 (fr) 2019-03-13
DK3348636T3 (da) 2022-02-28
EP3348636B1 (fr) 2021-12-01
CA2998087A1 (fr) 2017-03-16

Similar Documents

Publication Publication Date Title
HK1253540A1 (zh) 用於特異性轉變靶向dna序列的核酸碱基的基因組序列的修飾方法、及其使用的分子複合體
HK1253544A1 (zh) 通過特異性地轉化靶dna序列的核碱基轉化革蘭氏陽性細菌的基因組序列的方法，以及用於該方法的分子複合物
SG11201609211VA (en) Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
IL288263A (en) CRISPR DNA/RNA hybrid polynucleotides and methods for using them
HK1243463A1 (zh) 檢測rna病毒的組合物和方法
PT3216867T (pt) Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado
IL251450A0 (en) Preparations and methods for silencing hepatitis b virus gene expression
HK1248278A1 (zh) 用於使乙型肝炎病毒基因表達沉默的組合物及方法
SG11201608503UA (en) Methods and compositions for modifying genomic dna
GB201519584D0 (en) Nucleic acids, peptides and methods
EP3245220C0 (fr) Procédés et compositions destinés au transfert de gènes ciblé
HK1252166A1 (zh) 從環狀rna產生環狀dna的方法
HUE056009T2 (hu) DNS vektorok, transzpozonok és transzpozázok eukarióta genom módosítására
GB201518786D0 (en) Molecular and bioinformatics methods for direct sequencing
SG11201705820UA (en) Gene expression system using stealthy rna, and gene introduction/expression vector including said rna
ZA201707935B (en) Herbicide-tolerant maize plant dbn9858, and nucleotide sequence and method for detecting same
SG11201704744QA (en) Methods and kits for purifying plasmid dna
HK1201887A1 (en) Hybrid genome processing method