SG11201700891SA - Digital measurements from targeted sequencing - Google Patents

Digital measurements from targeted sequencing

Info

Publication number
SG11201700891SA
SG11201700891SA SG11201700891SA SG11201700891SA SG11201700891SA SG 11201700891S A SG11201700891S A SG 11201700891SA SG 11201700891S A SG11201700891S A SG 11201700891SA SG 11201700891S A SG11201700891S A SG 11201700891SA SG 11201700891S A SG11201700891S A SG 11201700891SA
Authority
SG
Singapore
Prior art keywords
targeted sequencing
digital measurements
measurements
digital
sequencing
Prior art date
Application number
SG11201700891SA
Other languages
English (en)
Inventor
Jonathan Scolnick
Benjamin Schroeder
Douglas Amorese
Stephanie C Huelga
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Publication of SG11201700891SA publication Critical patent/SG11201700891SA/en

Links

Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00ICT programming tools or database systems specially adapted for bioinformatics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Databases & Information Systems (AREA)
  • Bioethics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201700891SA 2014-08-06 2015-08-06 Digital measurements from targeted sequencing SG11201700891SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462034043P 2014-08-06 2014-08-06
PCT/US2015/044065 WO2016022833A1 (en) 2014-08-06 2015-08-06 Digital measurements from targeted sequencing

Publications (1)

Publication Number Publication Date
SG11201700891SA true SG11201700891SA (en) 2017-03-30

Family

ID=55264574

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201700891SA SG11201700891SA (en) 2014-08-06 2015-08-06 Digital measurements from targeted sequencing

Country Status (7)

Country Link
US (2) US10102337B2 (hr)
EP (1) EP3177740B1 (hr)
JP (1) JP6803327B2 (hr)
CN (1) CN107075581B (hr)
CA (1) CA2957633A1 (hr)
SG (1) SG11201700891SA (hr)
WO (1) WO2016022833A1 (hr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103748236B (zh) 2011-04-15 2018-12-25 约翰·霍普金斯大学 安全测序系统
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
PL2912468T3 (pl) 2012-10-29 2019-04-30 Univ Johns Hopkins Test papanicolaou pod kątem raka jajnika i endometrium
EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ, インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
WO2018176007A1 (en) * 2017-03-23 2018-09-27 BioSpyder Technologies, Inc. Modulation of targets in cellular pathways identified by resolution of stochastic gene expression
EP3332024B1 (en) 2015-08-06 2020-10-28 F.Hoffmann-La Roche Ag Target enrichment by single probe primer extension
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
DK3374525T3 (da) 2015-11-11 2021-04-06 Resolution Bioscience Inc Højeffektiv konstruktion af dna-biblioteker
WO2017210469A2 (en) 2016-06-01 2017-12-07 F. Hoffman-La Roche Ag Immuno-pete
CN106048009B (zh) * 2016-06-03 2020-02-18 人和未来生物科技(长沙)有限公司 一种用于超低频基因突变检测的标签接头及其应用
KR101969905B1 (ko) * 2016-06-20 2019-04-17 한국표준과학연구원 염기서열분석을 위한 라이브러리용 프라이머 세트 및 라이브러리 제조방법
EP3504347A4 (en) * 2016-08-25 2020-04-29 Resolution Bioscience, Inc. METHODS FOR DETECTING GENOMIC COPY CHANGES IN DNA SAMPLES
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
WO2018162538A1 (en) 2017-03-08 2018-09-13 F. Hoffmann-La Roche Ag Primer extension target enrichment and improvements thereto including simultaneous enrichment of dna and rna
WO2019078909A2 (en) * 2017-10-16 2019-04-25 The Regents Of The University Of California PREPARATION OF EFFECTIVE SCREENING LIBRARY
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP4289996A3 (en) * 2017-11-06 2024-01-17 Illumina Inc. Nucleic acid indexing techniques
EP3524695B1 (en) * 2018-02-13 2022-07-20 Personal Genomics S.r.l. Method for the enrichment of genomic regions
CA3106677A1 (en) * 2018-08-17 2020-02-20 Dana-Farber Cancer Institue, Inc. Dna amplification method for probe generation
CN110699426B (zh) * 2019-01-02 2022-01-28 上海臻迪基因科技有限公司 基因目标区域富集方法及试剂盒
CN113593636B (zh) * 2020-04-30 2024-05-03 深圳市真迈生物科技有限公司 测序结果分析方法、系统及计算机可读存储介质和电子设备
CN118139990A (zh) * 2021-10-18 2024-06-04 深圳市真迈生物科技有限公司 测序方法、测序数据处理方法、设备和计算机设备

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925065A (en) 1987-01-02 1990-05-15 Helena Laboratories Corporation Dispensing apparatus
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2013317A1 (en) 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
WO1992007951A1 (en) 1990-10-19 1992-05-14 Yoke Wah Kow Quantitation of aldehyde-containing lesions in nucleic acids
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
CA2131311C (en) 1992-03-05 2004-06-29 Phillip Dan Cook Covalently cross-linked oligonucleotides
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6110709A (en) 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2223104A1 (en) 1995-06-07 1996-12-19 Jonathan P. Duvick Pcr-based cdna subtractive cloning method
WO1997003210A1 (en) 1995-07-11 1997-01-30 FORFÁS (trading as BIORESEARCH IRELAND) Glycosylase mediated detection of nucleotide sequences at candidate loci
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
GB9600384D0 (en) 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
EP2264045B1 (en) 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
IL120337A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Method for joining DNA fragments
DE69837913T2 (de) 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden Verfahren zur vervielfältigung von nukleinsäure
JP2002511767A (ja) 1997-08-28 2002-04-16 ピーイー コーポレイション(エヌワイ) 化学切断による、核酸における変異の改良された検出
EP1009865A1 (en) 1997-08-29 2000-06-21 Osvaldo J. Lopez Dna methyltransferase genotyping
WO1999022030A1 (en) 1997-10-28 1999-05-06 The Regents Of The University Of California Dna polymorphism identity determination using flow cytometry
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
CA2325854C (en) 1998-04-22 2005-12-06 Enterprise Ireland Trading As Bioresearch Ireland A method for the characterisation of nucleic acid molecules involving generation of extendible upstream dna fragments resulting from the cleavage of nucleic acid at an abasic site
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
GB9817055D0 (en) 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CA2357601A1 (en) 1998-12-30 2000-07-06 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
CA2362771A1 (en) 1999-03-12 2000-09-21 Amersham Pharmacia Biotech Uk Limited Genetic analysis
WO2000070039A1 (en) 1999-05-17 2000-11-23 Mcgill University METHOD FOR SUBTRACTING cDNAs BY SUPPRESSING THE SYNTHESIS OF SPECIFICALLY TARGETED mRNAs
US6225109B1 (en) 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
JP3929775B2 (ja) 1999-09-13 2007-06-13 ニューゲン テクノロジーズ, インコーポレイテッド ポリヌクレオチド配列の線形等温増幅のための方法および組成物
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6617442B1 (en) 1999-09-30 2003-09-09 Isis Pharmaceuticals, Inc. Human Rnase H1 and oligonucleotide compositions thereof
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
AU2170901A (en) 1999-12-21 2001-07-03 Lion Bioscience Ag Branched compound for use in nucleic acid detection and analysis reactions
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
EP1356094B1 (en) 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002028876A2 (en) 2000-10-05 2002-04-11 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.
EP1356104A2 (en) 2000-10-06 2003-10-29 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6893820B1 (en) 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
WO2002072773A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
IL153504A0 (en) 2001-03-09 2003-07-06 Nugen Technologies Inc Methods and compositions for amplification of rna sequences
WO2002081753A1 (en) 2001-04-04 2002-10-17 Advanced Research & Technology Institute Method for identifying and characterizing individual dna molecules
FR2824335A1 (fr) 2001-05-04 2002-11-08 Bio Merieux Procede de marquage et de fragmentation d'adn
JP3967319B2 (ja) 2001-06-27 2007-08-29 エフ.ホフマン−ラ ロシュ アーゲー ポリヌクレオチド配列のインビトロ組換えのためのウォークスルー技術
US20060183115A1 (en) 2001-09-26 2006-08-17 Epigenx Pharmaceuticals Assays for dna methylation changes
JP2004148509A (ja) 2001-10-04 2004-05-27 Seiko Epson Corp 液体噴射ヘッド
ATE405676T1 (de) 2002-03-11 2008-09-15 Nugen Technologies Inc Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
JP2005521409A (ja) 2002-03-29 2005-07-21 ニューゲン テクノロジーズ, インコーポレイテッド 単一プライマー等温核酸増幅−増強型被分析物検出および定量
US6887709B2 (en) 2002-05-09 2005-05-03 Lifescan, Inc. Devices, systems and methods for the containment and use of liquid solutions
CA2486283A1 (en) 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
JP4473878B2 (ja) 2003-01-29 2010-06-02 454 コーポレーション 核酸を増幅および配列決定する方法
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
WO2005038427A2 (en) * 2003-06-05 2005-04-28 Medical Gene Center Ltd. Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005065321A2 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7361468B2 (en) 2004-07-02 2008-04-22 Affymetrix, Inc. Methods for genotyping polymorphisms in humans
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
JP2008513782A (ja) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子解析のための装置及び方法
WO2006055521A2 (en) 2004-11-16 2006-05-26 Helicos Biosciences Corporation Tirf single molecule analysis and method of sequencing nucleic acids
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7462468B1 (en) 2005-01-28 2008-12-09 Pacific Biosciences Of California, Inc. DNA intercalating agents and methods of use
EP1844328A4 (en) 2005-01-31 2009-09-16 Pacific Biosciences California USE OF REVERSIBLE EXTENSION TERMINATOR IN NUCLEIC ACID SEQUENCING
US20060286566A1 (en) 2005-02-03 2006-12-21 Helicos Biosciences Corporation Detecting apparent mutations in nucleic acid sequences
WO2006086668A2 (en) 2005-02-09 2006-08-17 Epicentre Technologies Compositions and methods employing 5'-phosphate-dependent nucleic acid exonucleases
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
US20090137415A1 (en) 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1957667A1 (en) 2005-11-15 2008-08-20 Solexa Ltd. Method of target enrichment
EP2010657A2 (en) 2006-04-24 2009-01-07 Nimblegen Systems, Inc. Use of microarrays for genomic representation selection
US20080269476A1 (en) 2006-04-26 2008-10-30 Helicos Biosciences Corporation Molecules and methods for nucleic acid sequencing
WO2007136717A1 (en) 2006-05-16 2007-11-29 Nugen Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
EP2038429B1 (en) 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
US8293501B2 (en) 2006-09-12 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8506908B2 (en) 2007-03-09 2013-08-13 Vantix Holdings Limited Electrochemical detection system
US7767805B2 (en) 2007-05-03 2010-08-03 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US9248076B2 (en) 2007-05-16 2016-02-02 Mystic Pharmaceuticals, Inc. Dose dispensing containers
CA2689626C (en) 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CL2008001682A1 (es) 2007-06-08 2008-12-12 Monsanto Technology Llc Metodos para el mejoramiento de plantas mediante el uso de informacion directa de secuencias de acidos nucleicos.
WO2009017678A2 (en) 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
WO2009102878A2 (en) 2008-02-12 2009-08-20 Nugen Technologies, Inc. Method for archiving and clonal expansion
GB0804690D0 (en) 2008-03-13 2008-04-16 Netherlands Cancer Inst The Method
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
EP3269824A1 (en) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US20100120034A1 (en) 2008-07-03 2010-05-13 Life Technologies Corporation Methylation analysis of mate pairs
JP5221248B2 (ja) 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のcDNAクローンの含有率を低減させたcDNAライブラリーの作製方法
CN102203273A (zh) 2008-09-09 2011-09-28 生命技术公司 生成基因特异性的文库的方法
WO2010039991A2 (en) 2008-10-02 2010-04-08 The Texas A&M University System Method of generating informative dna templates for high-throughput sequencing applications
CN102239258A (zh) 2008-12-04 2011-11-09 凯津公司 减少连接适配器的限制性片段中重复序列的方法
GB2465986A (en) 2008-12-04 2010-06-09 Angeletti P Ist Richerche Bio Method of generating diversity in polynucleotide sequences
CA2750547C (en) 2009-01-22 2018-07-03 Quanta Biosciences Methods for enrichment of selected rna molecules
EP2393717A4 (en) 2009-02-06 2013-08-14 Univ Northwestern PACKAGING LIQUID THAT CAN EXPLODE AND USES THEREOF
EP2789694A1 (en) 2009-04-02 2014-10-15 Fluidigm Corporation Microfluidic device with reaction product recovery system
WO2010129937A2 (en) 2009-05-08 2010-11-11 Life Technologies Corporation Methods for detecting genetic variations in dna samples
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
US20110224105A1 (en) 2009-08-12 2011-09-15 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
EP2475777A4 (en) 2009-09-11 2013-03-06 Nugen Technologies Inc COMPOSITIONS AND METHODS FOR COMPLETE TRANSCRIPTOMAL ANALYSIS
WO2011053987A1 (en) 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence selection and amplification
ITRM20100293A1 (it) 2010-05-31 2011-12-01 Consiglio Nazionale Ricerche Metodo per la preparazione e amplificazione di librerie rappresentative di cdna per il sequenziamento massivo, loro uso, kit e cartucce per kit di automazione
WO2011156529A2 (en) 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
GB201012748D0 (en) 2010-07-29 2010-09-15 Univ St Andrews Improved RACE
EP2623613B8 (en) 2010-09-21 2016-09-07 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
CA3080686C (en) 2010-10-22 2023-10-10 Cold Spring Harbor Laboratory Varietal counting of nucleic acids for obtaining genomic copy number information
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
AU2011323107B2 (en) 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
SG194745A1 (en) 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
CN104080958A (zh) 2011-10-19 2014-10-01 纽亘技术公司 用于定向核酸扩增和测序的组合物和方法
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
CN104093890B (zh) * 2012-01-26 2016-04-20 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
CA2867293C (en) 2012-03-13 2020-09-01 Abhijit Ajit PATEL Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
EP4148142A1 (en) 2012-05-21 2023-03-15 The Scripps Research Institute Methods of sample preparation
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ, インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US11261494B2 (en) * 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
WO2014028778A1 (en) * 2012-08-15 2014-02-20 Natera, Inc. Methods and compositions for reducing genetic library contamination
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
WO2014082032A1 (en) * 2012-11-26 2014-05-30 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
JP6570189B2 (ja) 2013-10-15 2019-09-04 ミスティック ファーマシューティカルズ, インコーポレイテッド 制御可能な速度の乱流形成ノズル
JP6525473B2 (ja) * 2013-11-13 2019-06-05 ニューゲン テクノロジーズ, インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
GB201320542D0 (en) 2013-11-21 2014-01-01 Randox Lab Ltd Assay fluid delivery system
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US9527077B2 (en) 2015-01-29 2016-12-27 David W. Wright Diagnostic cartridge, fluid storage and delivery apparatus therefor and methods of construction thereof

Also Published As

Publication number Publication date
JP2017526353A (ja) 2017-09-14
US20160203259A1 (en) 2016-07-14
EP3177740A4 (en) 2018-02-28
CN107075581B (zh) 2022-03-18
EP3177740B1 (en) 2021-01-13
WO2016022833A1 (en) 2016-02-11
CA2957633A1 (en) 2016-02-11
EP3177740A1 (en) 2017-06-14
CN107075581A (zh) 2017-08-18
US20190005193A1 (en) 2019-01-03
JP6803327B2 (ja) 2020-12-23
US10102337B2 (en) 2018-10-16

Similar Documents

Publication Publication Date Title
SG11201700891SA (en) Digital measurements from targeted sequencing
HK1245830A1 (zh) 測序對照
IL247624A0 (en) Intentional healing
GB201517022D0 (en) No details
IL250836B (en) Nucleotide analogs
GB201410907D0 (en) No details
GB201519531D0 (en) No details
GB201517404D0 (en) No details
GB201418217D0 (en) No details
GB201418216D0 (en) No details
GB201517412D0 (en) No details
GB201418144D0 (en) High throughput sequencing
GB201516796D0 (en) No details
GB201517574D0 (en) No details
IL246956A0 (en) targeted therapies
GB2533151B (en) Improved probe
PL3237349T3 (pl) Ulepszony biologiczny środek wiążący
GB201517605D0 (en) No details
GB201403515D0 (en) Thermometer
GB201419494D0 (en) Variations
GB201410910D0 (en) No details
HK1198317A2 (en) Digital tweaker scale elite
SG11201700015WA (en) Improved apparatus
GB201801971D0 (en) No details
GB201604705D0 (en) No details