SG11201601720RA - New method for inducing dopamine-producing neural precursor cells - Google Patents

New method for inducing dopamine-producing neural precursor cells

Info

Publication number
SG11201601720RA
SG11201601720RA SG11201601720RA SG11201601720RA SG11201601720RA SG 11201601720R A SG11201601720R A SG 11201601720RA SG 11201601720R A SG11201601720R A SG 11201601720RA SG 11201601720R A SG11201601720R A SG 11201601720RA SG 11201601720R A SG11201601720R A SG 11201601720RA
Authority
SG
Singapore
Prior art keywords
new method
precursor cells
neural precursor
producing neural
inducing dopamine
Prior art date
Application number
SG11201601720RA
Other languages
English (en)
Inventor
Jun Takahashi
Daisuke Doi
Bumpei Samata
Kiyotoshi Sekiguchi
Yuichi Ono
Original Assignee
Univ Kyoto
Eisai R&D Man Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kyoto, Eisai R&D Man Co Ltd filed Critical Univ Kyoto
Publication of SG11201601720RA publication Critical patent/SG11201601720RA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0623Stem cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/14Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16Anti-Parkinson drugs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0619Neurons
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/119Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/13Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/41Hedgehog proteins; Cyclopamine (inhibitor)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases (EC 2.)
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Neurosurgery (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Immunology (AREA)
  • Psychology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
SG11201601720RA 2013-09-05 2014-09-04 New method for inducing dopamine-producing neural precursor cells SG11201601720RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013184387 2013-09-05
PCT/JP2014/073372 WO2015034012A1 (ja) 2013-09-05 2014-09-04 新規ドーパミン産生神経前駆細胞の誘導方法

Publications (1)

Publication Number Publication Date
SG11201601720RA true SG11201601720RA (en) 2016-04-28

Family

ID=52628477

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201601720RA SG11201601720RA (en) 2013-09-05 2014-09-04 New method for inducing dopamine-producing neural precursor cells

Country Status (12)

Country Link
US (2) US11473058B2 (de)
EP (1) EP3042951B1 (de)
JP (3) JP6558580B2 (de)
KR (1) KR102320537B1 (de)
CN (1) CN105849255A (de)
AU (1) AU2014316100B2 (de)
CA (1) CA2923592A1 (de)
DK (1) DK3042951T3 (de)
ES (1) ES2721440T3 (de)
MY (1) MY184219A (de)
SG (1) SG11201601720RA (de)
WO (1) WO2015034012A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8642334B2 (en) 2009-02-17 2014-02-04 Memorial Sloan Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
KR102426327B1 (ko) 2011-11-04 2022-08-01 메모리얼 슬로안 케터링 캔서 센터 생착용 중뇌 도파민(da) 뉴런
AU2014256876B2 (en) 2013-04-26 2020-11-12 Cornell, University Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
CN107438669B (zh) * 2015-04-09 2022-09-27 拜奥拉米那公司 用于治疗神经退行性疾病的干细胞衍生多巴胺能细胞的生产方法和组合物
KR102358878B1 (ko) * 2015-06-01 2022-02-08 메모리얼 슬로안 케터링 캔서 센터 중간뇌 도파민(mda) 뉴런의 시험관내 분화 방법
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
JP7306667B2 (ja) * 2016-03-31 2023-07-12 慶應義塾 分化促進型多能性幹細胞及びその使用
US11898163B2 (en) * 2016-04-22 2024-02-13 Kyoto University Method for producing dopaminergic neuron progenitor cell
CN106479980B (zh) * 2016-07-04 2019-12-31 上海爱萨尔生物科技有限公司 神经干细胞培养液及培养方法
US11959103B2 (en) 2016-11-11 2024-04-16 Osaka University Method for inducing pluripotent stem cells to differentiate into somatic cells
JPWO2018193949A1 (ja) * 2017-04-19 2020-02-27 国立大学法人名古屋大学 ドパミン神経細胞の調製方法
EP3757208A4 (de) * 2018-02-19 2021-12-01 Sumitomo Dainippon Pharma Co., Ltd. Zellenaggregat, mischung von zellaggregaten und verfahren zur herstellung davon
CN114606188A (zh) * 2019-03-06 2022-06-10 安徽中盛溯源生物科技有限公司 一种中脑多巴胺能神经前体细胞的制备方法及其应用
JP7483233B2 (ja) * 2019-03-29 2024-05-15 国立大学法人 長崎大学 培養組織及びその製造方法
CN110872576A (zh) * 2019-06-06 2020-03-10 中国科学院广州生物医药与健康研究院 一种将小鼠成纤维细胞转分化为多巴胺能神经元的方法
WO2021042027A1 (en) * 2019-08-29 2021-03-04 Memorial Sloan-Kettering Cancer Center Methods of generating and isolating midbrain dopamine neurons
CA3155277A1 (en) * 2019-09-20 2021-03-25 Korea Research Institute Of Bioscience And Biotechnology Production method for induced dopaminergic neuronal progenitors, using direct reprogramming
KR102392133B1 (ko) 2019-09-25 2022-04-29 (주) 에스바이오메딕스 줄기세포로부터 도파민 신경전구세포 분화 유도 방법
AU2020202273B2 (en) * 2019-09-25 2022-06-30 S-Biomedics Method for inducing differentiation of stem cell into dopaminergic neural precursor cell
CN112852735A (zh) * 2019-11-12 2021-05-28 光武惠文生物科技(北京)有限公司 诱导诱导性多能干细胞分化成神经前体细胞的方法
US20220408717A1 (en) 2019-11-20 2022-12-29 Sumitomo Pharma Co., Ltd. Method for freezing cell aggregates
US20230000071A1 (en) 2019-11-20 2023-01-05 Sumitomo Pharma Co., Ltd. Method for freezing neural cells
EP4090734A1 (de) * 2020-01-13 2022-11-23 Aspen Neuroscience, Inc. Verfahren zur differenzierung von neuralen zellen sowie zugehörige zusammensetzungen und verwendungsverfahren
WO2021216622A1 (en) * 2020-04-21 2021-10-28 Aspen Neuroscience, Inc. Gene editing of gba1 in stem cells and method of use of cells differentiated therefrom
WO2021216623A1 (en) * 2020-04-21 2021-10-28 Aspen Neuroscience, Inc. Gene editing of lrrk2 in stem cells and method of use of cells differentiated therefrom
US20230201376A1 (en) * 2020-05-01 2023-06-29 The Trustees Of Indiana University Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy
KR20220126211A (ko) 2021-03-07 2022-09-15 석 영 정 하이브리드 전력 연계 시스템
CN113789301B (zh) * 2021-08-27 2022-05-10 广州瑞臻再生医学科技有限公司 一种制备高纯度的诱导性多能干细胞来源的人脑多巴胺能神经元的方法
WO2023153464A1 (ja) * 2022-02-09 2023-08-17 住友ファーマ株式会社 多能性幹細胞から中脳底板領域の神経系細胞への分化における、培養液中の細胞の分化能を判定する方法
WO2024113351A1 (en) * 2022-12-02 2024-06-06 Nuwacell Biotechnologies Co., Ltd. Culture medium, coating matrix and method for maturing midbrain dopaminergic progenitor cells

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU6319901A (en) * 2000-05-17 2001-11-26 Geron Corp Neural progenitor cell populations
ATE353889T1 (de) 2001-03-23 2007-03-15 Bayer Pharmaceuticals Corp Rho-kinase inhibitoren
PE20021011A1 (es) 2001-03-23 2003-02-01 Bayer Corp Derivados quinazolinicos como inhibidores de la rho-quinasa
EP1465900B1 (de) 2002-01-10 2008-05-14 Bayer HealthCare AG Rho-kinase inhibitoren
US6943172B2 (en) 2002-01-23 2005-09-13 Bayer Pharmaceuticals Corporation Rho-kinase inhibitors
JP4469179B2 (ja) 2002-01-23 2010-05-26 バイエル ファーマセチカル コーポレーション Rhoキナーゼ阻害剤としてのピリミジン誘導体
EP1562935B1 (de) 2002-10-28 2006-09-06 Bayer HealthCare AG Heteroaryloxy-substituierte phenylaminopyrimidine als rho-kinaseinhibitoren
JP4573332B2 (ja) 2003-01-24 2010-11-04 エーザイ・アール・アンド・ディー・マネジメント株式会社 Lrp4/Corinドーパミン産生ニューロン増殖前駆細胞マーカー
BRPI0408733A (pt) 2003-03-12 2006-03-07 Reliance Life Sciences Pvt Ltd derivação de neurÈnios dopaminérgicos terminalmente diferenciados de células tronco embriÈnicas humanas
ZA200401646B (en) * 2003-03-12 2004-06-07 Reliance Life Sciences Pvt Ltd Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells.
US9453219B2 (en) 2003-05-15 2016-09-27 Mello Biotech Taiwan Co., Ltd. Cosmetic designs and products using intronic RNA
CN1906309B (zh) 2003-11-26 2011-12-14 卫材R&D管理有限公司 特异于产生多巴胺的神经元的标记物Lmx1a
US20080199437A1 (en) 2004-07-22 2008-08-21 Eisai Co., Ltd. Lrp4/Corin Dopaminergic Neuron Progenitor Cell Markers
EP2206724A1 (de) 2005-12-13 2010-07-14 Kyoto University Nukleärer Reprogramierungsfaktor
CN101466836A (zh) 2006-04-11 2009-06-24 卫材R&D管理有限公司 多巴胺能神经元祖细胞标志物187a5
EP2137296A2 (de) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Neuprogrammierung von körperzellen
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
ES2589122T3 (es) 2007-08-31 2016-11-10 Whitehead Institute For Biomedical Research Estimulación de la ruta de la Wnt en la reprogramación de células somáticas
WO2009057831A1 (ja) 2007-10-31 2009-05-07 Kyoto University 核初期化方法
AU2008286249B2 (en) 2007-12-10 2013-10-10 Kyoto University Efficient method for nuclear reprogramming
AU2008338989A1 (en) 2007-12-17 2009-06-25 Gliamed, Inc. Stem-like cells and method for reprogramming adult mammalian somatic cells
CN101970664B (zh) 2008-01-16 2013-08-21 林希龙 使用可诱导的重组核糖核酸因子生成不含肿瘤的类胚胎干细胞的多能性细胞
EP2250252A2 (de) 2008-02-11 2010-11-17 Cambridge Enterprise Limited Verbesserte neuprogrammierung von säugerzellen und damit erhaltene zellen
EP2090649A1 (de) 2008-02-13 2009-08-19 Fondazione Telethon Verfahren zur Umprogrammierung differenzierter Zellen
WO2009102983A2 (en) 2008-02-15 2009-08-20 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
AU2009225665B9 (en) 2008-03-17 2015-01-15 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
WO2009114949A1 (en) 2008-03-20 2009-09-24 UNIVERSITé LAVAL Methods for deprogramming somatic cells and uses thereof
US20110117645A1 (en) 2008-03-31 2011-05-19 Oriental Yeast Co., Ltd. Method for proliferation of pluripotent stem cells
CN102083968A (zh) 2008-04-07 2011-06-01 纽珀滕索有限公司 通过使用小分子调节剂诱导复能基因而重编程细胞
JP2011521969A (ja) 2008-05-30 2011-07-28 スムマ ヘルス システムズ エルエルシー 眼疾患を治療するためのTGF−β受容体阻害剤又はアクチビン様キナーゼ(ALK)5阻害剤、A−83−01及びSB−431542の使用方法及び創傷治療条件
SG10201402428WA (en) 2008-06-27 2014-07-30 Univ Kyoto Method of efficiently establishing induced pluripotent stem cells
WO2010009015A2 (en) 2008-07-14 2010-01-21 Oklahoma Medical Research Foundation Production of pluripotent cells through inhibition of bright/arid3a function
EP3075850B1 (de) 2008-07-16 2019-02-06 ID Pharma Co., Ltd. Verfahren zur herstellung einer neuprogrammierten zelle anhand eines chromosomal unintegrierten virusvektors
JP5562231B2 (ja) 2008-07-30 2014-07-30 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2010147612A1 (en) 2009-06-18 2010-12-23 Lixte Biotechnology, Inc. Methods of modulating cell regulation by inhibiting p53
WO2010033920A2 (en) 2008-09-19 2010-03-25 Whitehead Institute For Biomedical Research Compositions and methods for enhancing cell reprogramming
US20120021519A1 (en) 2008-09-19 2012-01-26 Presidents And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
WO2010042800A1 (en) 2008-10-10 2010-04-15 Nevada Cancer Institute Methods of reprogramming somatic cells and methods of use for such cells
WO2010050626A1 (en) 2008-10-30 2010-05-06 Kyoto University Method for producing induced pluripotent stem cells
US20110059526A1 (en) 2008-11-12 2011-03-10 Nupotential, Inc. Reprogramming a cell by inducing a pluripotent gene through use of an hdac modulator
WO2010068955A2 (en) 2008-12-13 2010-06-17 Dna Microarray MICROENVIRONMENT NICHE ASSAY FOR CiPS SCREENING
EP2401372A4 (de) 2009-02-27 2012-09-19 Univ Kyoto Neuer nuklearer neuprogrammierungsstoff
US20120122212A1 (en) 2009-03-06 2012-05-17 Marica Grskovic Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells
US9340775B2 (en) 2009-03-25 2016-05-17 The Salk Institute For Biological Studies Induced pluripotent stem cell produced by transfecting a human neural stem cell with an episomal vector encoding the Oct4 and Nanog proteins
US20120076762A1 (en) 2009-03-25 2012-03-29 The Salk Institute For Biological Studies Induced pluripotent stem cell generation using two factors and p53 inactivation
WO2010115050A2 (en) 2009-04-01 2010-10-07 The Regents Of The University Of California Embryonic stem cell specific micrornas promote induced pluripotency
WO2010124290A2 (en) 2009-04-24 2010-10-28 Whitehead Institute For Biomedical Research Compositions and methods for deriving or culturing pluripotent cells
JP5765714B2 (ja) 2009-05-29 2015-08-19 国立大学法人京都大学 人工多能性幹細胞の製造方法および培養方法
WO2010147395A2 (en) 2009-06-16 2010-12-23 Korea Research Institute Of Bioscience And Biotechnology Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
US9550975B2 (en) 2009-07-15 2017-01-24 Mari Dezawa SSEA-3 pluripotent stem cell isolated from body tissue
JP5590646B2 (ja) 2009-10-08 2014-09-17 国立大学法人大阪大学 ヒト多能性幹細胞用培養基材およびその利用
KR101168053B1 (ko) 2009-11-06 2012-07-24 연세대학교 산학협력단 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법
KR20120093407A (ko) * 2009-12-02 2012-08-22 리서치 디벨럽먼트 파운데이션 제한된 분화력을 가진 줄기세포 클론의 선별
JP6196213B2 (ja) * 2011-05-20 2017-09-13 ザ マクレーン ホスピタル コーポレーション ニューロン前駆細胞および使用法
KR101331034B1 (ko) * 2011-06-09 2013-11-19 연세대학교 산학협력단 미분화 줄기세포로부터 중뇌특이적 도파민 신경세포의 제조 방법
WO2013015457A1 (en) * 2011-07-27 2013-01-31 Kyoto University Novel markers for dopaminergic neuron progenitor cells

Also Published As

Publication number Publication date
US11473058B2 (en) 2022-10-18
CN105849255A (zh) 2016-08-10
JP2021180675A (ja) 2021-11-25
JPWO2015034012A1 (ja) 2017-03-02
JP6933843B2 (ja) 2021-09-08
JP2019141087A (ja) 2019-08-29
CA2923592A1 (en) 2015-03-12
KR20160053936A (ko) 2016-05-13
ES2721440T3 (es) 2019-07-31
JP6558580B2 (ja) 2019-08-14
EP3042951A4 (de) 2017-03-22
DK3042951T3 (en) 2019-04-15
EP3042951A1 (de) 2016-07-13
EP3042951B1 (de) 2019-02-20
MY184219A (en) 2021-03-26
WO2015034012A1 (ja) 2015-03-12
AU2014316100A1 (en) 2016-04-14
JP7282304B2 (ja) 2023-05-29
KR102320537B1 (ko) 2021-11-01
AU2014316100B2 (en) 2020-11-05
US20230114089A1 (en) 2023-04-13
US20160215260A1 (en) 2016-07-28

Similar Documents

Publication Publication Date Title
SG11201601720RA (en) New method for inducing dopamine-producing neural precursor cells
IL241627B (en) A method for t-cell activation
HK1219502A1 (zh) 片狀細胞培養物的製造方法
GB2510821B (en) Charging Method
HUE046414T2 (hu) Eljárás tagatóz elõállítására
EP3018660A4 (de) Kristalliner festelektrolyt und herstellungsverfahren dafür
EP2915906A4 (de) Verfahren zur herstellung einer elektrode für die elektrolyse
EP2892101A4 (de) Verfahren zur herstellung einer elektrodenanordnung
EP3001493A4 (de) Elektrodenanordnungsherstellungsverfahren
EP2892102A4 (de) Verfahren zur herstellung einer elektrodenanordnung
HUE036298T2 (hu) Eljárás és berendezés csomagolás elõállítására
EP2980066A4 (de) Verfahren zur herstellung von isocyanat
EP3070779A4 (de) Verfahren zur herstellung einer folienverpackten zelle
PL2882028T3 (pl) Sposób wytwarzania zespołu elektrodowego
EP2863466A4 (de) Elektrodenanordnung und verfahren zur herstellung einer elektrodenanordnung
EP2960338A4 (de) Zuckerlösungherstellungsverfahren
EP3024081A4 (de) Verfahren zur herstellung von zellen
EP2947046A4 (de) Verfahren zur herstellung von wasserstoff
GB2512711B (en) Graphene production method
IL241287A0 (en) Processes for the preparation of subfervir
EP2868476A4 (de) Herstellungsverfahren für drucksachen
HUE037846T2 (hu) Eljárás izocianátok elõállítására
AP2015008829A0 (en) Machine and method for gravitational-magnetic electricity production
PL2980053T3 (pl) Sposób wytwarzania butadienu
GB201322992D0 (en) New Process