SG11201400833XA - Cell culture substrate comprising a laminin and a cadherin - Google Patents

Cell culture substrate comprising a laminin and a cadherin

Info

Publication number
SG11201400833XA
SG11201400833XA SG11201400833XA SG11201400833XA SG11201400833XA SG 11201400833X A SG11201400833X A SG 11201400833XA SG 11201400833X A SG11201400833X A SG 11201400833XA SG 11201400833X A SG11201400833X A SG 11201400833XA SG 11201400833X A SG11201400833X A SG 11201400833XA
Authority
SG
Singapore
Prior art keywords
cadherin
laminin
cell culture
culture substrate
substrate
Prior art date
Application number
SG11201400833XA
Other languages
English (en)
Inventor
Karl Tryggvason
Sergey Rodin
Original Assignee
Karl Tryggvason
Sergey Rodin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karl Tryggvason, Sergey Rodin filed Critical Karl Tryggvason
Publication of SG11201400833XA publication Critical patent/SG11201400833XA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Reproductive Health (AREA)
  • Immunology (AREA)
  • Transplantation (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11201400833XA 2011-09-22 2012-09-19 Cell culture substrate comprising a laminin and a cadherin SG11201400833XA (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201161537940P 2011-09-22 2011-09-22
US201161565380P 2011-11-30 2011-11-30
US201161565849P 2011-12-01 2011-12-01
US201261624588P 2012-04-16 2012-04-16
US201261625321P 2012-04-17 2012-04-17
PCT/IB2012/002214 WO2013041961A1 (en) 2011-09-22 2012-09-19 Cell culture substrate comprising a laminin and a cadherin

Publications (1)

Publication Number Publication Date
SG11201400833XA true SG11201400833XA (en) 2014-04-28

Family

ID=47324206

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201704803RA SG10201704803RA (en) 2011-09-22 2012-09-19 Cell culture substrate comprising a laminin and a cadherin
SG11201400833XA SG11201400833XA (en) 2011-09-22 2012-09-19 Cell culture substrate comprising a laminin and a cadherin

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201704803RA SG10201704803RA (en) 2011-09-22 2012-09-19 Cell culture substrate comprising a laminin and a cadherin

Country Status (11)

Country Link
US (1) US10240124B2 (de)
EP (2) EP2758427B1 (de)
JP (1) JP6049733B2 (de)
KR (1) KR102048464B1 (de)
CN (1) CN104364263B (de)
AU (2) AU2012311233B2 (de)
CA (1) CA2849244C (de)
DK (1) DK3144319T3 (de)
IL (1) IL231630B (de)
SG (2) SG10201704803RA (de)
WO (1) WO2013041961A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL301479A (en) 2009-11-17 2023-05-01 Astellas Inst For Regenerative Medicine Methods for preparing human RPE cells and pharmaceutical preparations of human RPE cells
WO2013156855A1 (en) * 2012-04-20 2013-10-24 Biolamina Ab Maintenance of differentiated cells with laminins
US10226487B2 (en) * 2012-07-12 2019-03-12 Isletone Ab Derivation and self-renewal of lsl1+ cells and uses thereof
US9719066B2 (en) 2012-09-07 2017-08-01 Biolamina Ab Stem cell bank
CN105378054B (zh) * 2013-06-12 2018-09-21 国立大学法人大阪大学 以干燥状态包被有层粘连蛋白片段的细胞培养器具
WO2015006725A2 (en) 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
SI3059307T2 (sl) * 2015-02-20 2022-08-31 Inserm(Institut National De La Sante Et De La Recherche Medicale) Uporaba laminina za diferenciacijo pluripotentnih celic v hepatocite
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11999972B2 (en) * 2016-07-29 2024-06-04 Takara Bio Inc. Fibronectin fragment to be used for stem cell production
JP7078934B2 (ja) * 2016-08-25 2022-06-02 国立大学法人京都大学 特定のラミニン上での多能性幹細胞の培養方法
WO2018090084A1 (en) * 2016-11-16 2018-05-24 Cynata Therapeutics Limited Pluripotent stem cell assay
CN109125805A (zh) * 2018-09-06 2019-01-04 京和生殖医学技术(沈阳)有限公司 一种干细胞复合plga支架试剂盒及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508302A (ja) * 1997-01-10 2001-06-26 ライフ テクノロジーズ,インコーポレイテッド 胚性幹細胞血清置換
WO2002050111A2 (en) * 2000-12-21 2002-06-27 Biostratum, Inc. Isolated laminin 10
JP4613069B2 (ja) 2002-12-16 2011-01-12 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド 支持細胞非含有、異種非含有のヒト胚性幹細胞の調製方法およびこれらを使用して調製された幹細胞培養物
JP2006087396A (ja) * 2004-09-27 2006-04-06 National Institute For Environmental Studies 細胞培養基質及びその製造方法
CN101605885B (zh) * 2007-01-04 2013-03-27 卡尔·特吕格瓦松 能够使多能干细胞增殖的组合物及方法
US10829733B2 (en) 2007-01-04 2020-11-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
EP3192865B1 (de) 2007-07-01 2021-04-21 Janssen Biotech, Inc. Kultur einzelner pluripotenter stammzellen
EP2315827A1 (de) 2008-07-07 2011-05-04 Mass Institute Of Technology Mikromuster-cokultursysteme als analyseplattformen für infektionskrankheiten
BRPI0922345B1 (pt) * 2008-12-03 2022-03-22 The Scripps Research Institute Composto, métodos in vitro para estabilizar uma célula animal isolada e para manter a sobrevivência da célula, e, composição
US9045738B2 (en) 2009-05-29 2015-06-02 Kyoto University Method for producing induced pluripotent stem cells and method for culturing the same
US20100317916A1 (en) 2009-06-12 2010-12-16 Scott Jr Richard T Method for relative quantitation of chromosomal DNA copy number in single or few cells
JP5990531B2 (ja) * 2010-12-17 2016-09-14 ビオラミナ アーベー 組換えラミニン−521
US9133266B2 (en) * 2011-05-06 2015-09-15 Wisconsin Alumni Research Foundation Vitronectin-derived cell culture substrate and uses thereof

Also Published As

Publication number Publication date
US10240124B2 (en) 2019-03-26
AU2017204108A1 (en) 2017-07-06
AU2017204108B2 (en) 2018-11-15
US20140315306A1 (en) 2014-10-23
JP2014526271A (ja) 2014-10-06
EP2758427B1 (de) 2016-11-30
EP2758427A1 (de) 2014-07-30
EP3144319A1 (de) 2017-03-22
WO2013041961A1 (en) 2013-03-28
CN104364263A (zh) 2015-02-18
DK3144319T3 (da) 2019-08-05
AU2012311233A1 (en) 2014-04-10
JP6049733B2 (ja) 2016-12-21
KR20140092819A (ko) 2014-07-24
CN104364263B (zh) 2018-06-29
EP3144319B1 (de) 2019-05-22
IL231630B (en) 2019-10-31
EP3144319B8 (de) 2019-07-03
CA2849244C (en) 2023-10-10
AU2012311233B2 (en) 2017-03-16
KR102048464B1 (ko) 2019-11-25
IL231630A0 (en) 2014-05-28
CA2849244A1 (en) 2013-03-28
SG10201704803RA (en) 2017-07-28

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