SE452371B - Elektroforetiskt forfarande - Google Patents
Elektroforetiskt forfarandeInfo
- Publication number
- SE452371B SE452371B SE8205386A SE8205386A SE452371B SE 452371 B SE452371 B SE 452371B SE 8205386 A SE8205386 A SE 8205386A SE 8205386 A SE8205386 A SE 8205386A SE 452371 B SE452371 B SE 452371B
- Authority
- SE
- Sweden
- Prior art keywords
- electrophoretic
- acid
- gel
- group
- polysaccharide
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 10
- 239000000499 gel Substances 0.000 claims description 41
- 238000001962 electrophoresis Methods 0.000 claims description 16
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 12
- 108010044467 Isoenzymes Proteins 0.000 claims description 11
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 11
- 229920001284 acidic polysaccharide Polymers 0.000 claims description 11
- 229920000936 Agarose Polymers 0.000 claims description 10
- 150000004805 acidic polysaccharides Chemical class 0.000 claims description 10
- QXKAIJAYHKCRRA-JJYYJPOSSA-N D-arabinonic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C(O)=O QXKAIJAYHKCRRA-JJYYJPOSSA-N 0.000 claims description 9
- -1 alkyl polyol Chemical class 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- 150000004804 polysaccharides Chemical class 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 5
- CKLJMWTZIZZHCS-UWTATZPHSA-N D-aspartic acid Chemical compound OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 claims description 4
- 229920005862 polyol Polymers 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical class C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 241000557752 Khaya Species 0.000 claims description 3
- 125000004432 carbon atom Chemical group C* 0.000 claims description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 235000004426 flaxseed Nutrition 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical class [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 claims description 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims description 2
- 240000006240 Linum usitatissimum Species 0.000 claims description 2
- 235000004431 Linum usitatissimum Nutrition 0.000 claims description 2
- 229920002230 Pectic acid Polymers 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical class [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 229960005261 aspartic acid Drugs 0.000 claims description 2
- 239000011575 calcium Chemical class 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 claims description 2
- 239000010318 polygalacturonic acid Substances 0.000 claims description 2
- 239000011591 potassium Chemical class 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical class [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 238000005370 electroosmosis Methods 0.000 claims 1
- 239000011777 magnesium Chemical class 0.000 claims 1
- 229910052749 magnesium Chemical class 0.000 claims 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 229920001615 Tragacanth Polymers 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 description 1
- UXFQFBNBSPQBJW-UHFFFAOYSA-N 2-amino-2-methylpropane-1,3-diol Chemical compound OCC(N)(C)CO UXFQFBNBSPQBJW-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 241000416162 Astragalus gummifer Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000305 astragalus gummifer gum Substances 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 150000002896 organic halogen compounds Chemical class 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000196 tragacanth Substances 0.000 description 1
- 235000010487 tragacanth Nutrition 0.000 description 1
- 229940116362 tragacanth Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/226,571 US4321121A (en) | 1981-01-19 | 1981-01-19 | Electrophoretic technique for separating lactate dehydrogenase isoenzymes and improved electrophoretic gel for use therein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE8205386D0 SE8205386D0 (sv) | 1982-09-20 |
| SE8205386L SE8205386L (sv) | 1982-09-20 |
| SE452371B true SE452371B (sv) | 1987-11-23 |
Family
ID=22849448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8205386A SE452371B (sv) | 1981-01-19 | 1982-09-20 | Elektroforetiskt forfarande |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4321121A (it) |
| JP (1) | JPH0221745B2 (it) |
| DE (1) | DE3231628C2 (it) |
| IT (1) | IT1149706B (it) |
| SE (1) | SE452371B (it) |
| WO (1) | WO1982002600A1 (it) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61500810A (ja) * | 1984-03-12 | 1986-04-24 | ベツクマン インスツルメンツ インコ−ポレ−テツド | ヘモグロビン変異体の分離のための電気泳動方法およびそれに使用する改良電気泳動ゲル |
| JPS6266153A (ja) * | 1985-09-18 | 1987-03-25 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| US5665216A (en) * | 1986-10-21 | 1997-09-09 | Northeastern University | Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same |
| US4997537A (en) * | 1986-10-21 | 1991-03-05 | Northeastern University | High performance microcapillary gel electrophoresis |
| US4857163A (en) * | 1987-07-17 | 1989-08-15 | Beckman Instruments, Inc. | High resolution electrophoretic gel and method for separating serum proteins |
| US5230832A (en) * | 1991-01-24 | 1993-07-27 | Brandeis University | Galactomannan-agarose binary gel for nucleic acid electrophoresis |
| US5211574A (en) * | 1992-03-13 | 1993-05-18 | Molex Incorporated | High density electrical connector assembly with improved alignment/guide means |
| US5371208A (en) * | 1992-12-30 | 1994-12-06 | Guest Elchrom Scientific Ltd. | Preparation of cross-linked linear polysaccharide polymers as gels for electrophoresis |
| WO1997007395A1 (en) * | 1995-08-21 | 1997-02-27 | Trevigen, Inc. | Separation medium for capillary electrophoresis |
| DE102013105131A1 (de) | 2013-05-17 | 2014-11-20 | Dbk David + Baader Gmbh | Blowby-Einrichtung |
| JP2021028605A (ja) * | 2019-08-09 | 2021-02-25 | 国立大学法人広島大学 | 電気泳動用ゲルおよびその利用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3527712A (en) * | 1967-03-07 | 1970-09-08 | Marine Colloids Inc | Dried agarose gel,method of preparation thereof,and production of aqueous agarose gel |
| US3959251A (en) * | 1970-06-25 | 1976-05-25 | Exploaterings Aktiebolaget T.B.F. | Stabilized agar product and method for its stabilization |
| US3956273A (en) * | 1971-06-07 | 1976-05-11 | Marine Colloids, Inc. | Modified agarose and agar and method of making same |
| GB1435508A (en) * | 1972-05-01 | 1976-05-12 | Rech Et Dapplications Scient S | Process for the preparation of crosslinked polysaccharide gels |
| US3947352A (en) * | 1974-05-31 | 1976-03-30 | Pedro Cuatrecasas | Polysaccharide matrices for use as adsorbents in affinity chromatography techniques |
| SE7908454L (sv) * | 1979-10-11 | 1981-04-12 | Pharmacia Fine Chemicals Ab | Medium for isoelektrisk fokusering |
| US4305798A (en) * | 1980-04-11 | 1981-12-15 | Bryce Allen Cunningham | Method of preparative, Kohlrausch-regulated electrophoresis using polyethylene glycol derivatized trailing ions |
-
1981
- 1981-01-19 US US06/226,571 patent/US4321121A/en not_active Expired - Lifetime
-
1982
- 1982-01-11 WO PCT/US1982/000024 patent/WO1982002600A1/en not_active Ceased
- 1982-01-11 DE DE19823231628 patent/DE3231628C2/de not_active Expired
- 1982-01-11 JP JP57500726A patent/JPH0221745B2/ja not_active Expired
- 1982-01-19 IT IT19191/82A patent/IT1149706B/it active
- 1982-09-20 SE SE8205386A patent/SE452371B/sv unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO1982002600A1 (en) | 1982-08-05 |
| DE3231628C2 (de) | 1987-06-04 |
| JPS57502097A (it) | 1982-11-25 |
| JPH0221745B2 (it) | 1990-05-16 |
| IT8219191A0 (it) | 1982-01-19 |
| SE8205386D0 (sv) | 1982-09-20 |
| SE8205386L (sv) | 1982-09-20 |
| DE3231628T1 (de) | 1983-09-22 |
| IT1149706B (it) | 1986-12-10 |
| US4321121A (en) | 1982-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SE452371B (sv) | Elektroforetiskt forfarande | |
| EP0029284B1 (en) | Method for the electrophoretic separation of proteins, a buffer solution and buffer compositions therefor | |
| DE69908795D1 (de) | Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür | |
| DK153585A (da) | Middel, testmateriale og fremgangsmaade til bestemmelse af tilstedevaerelsen af leukocyter, esterase eller protease | |
| SE452197B (sv) | Elektroforetisk gel samt anvendning av densamma | |
| US3497437A (en) | Method of electrophoresis | |
| Rodwell et al. | Association of protein and lipid in Mycoplasma laidlawii membranes disaggregated by detergents | |
| DK155953C (da) | Fremgangmsaade til stabilisering af peroxidase i et flydende medium og saaledes stabiliseret medium | |
| Malik et al. | Molecular properties of rat intestinal alkaline phosphatase | |
| US4808288A (en) | Electrophoretic gel for separating hemoglobin variants | |
| EP0183711A1 (en) | Electrophoretic technique for separating hemoglobin variants and improved electrophoretic gel for use therein | |
| EP0189422B1 (en) | Electrophoretic technique for separating hemoglobin variants and electrophoretic gel for use therein | |
| US4696958A (en) | Electrophoretic technique for separation of lipoproteins and electrophoretic gel for use therein | |
| WO2000028314A9 (en) | Methods and formulations for the separation of biological macromolecules | |
| US4321119A (en) | Buffer composition and method for the electrophoretic separation of proteins | |
| Gallagher et al. | Phenol-acetic acid-urea polyacrylamide gel electrophoresis of membrane proteins | |
| Di Luccia et al. | Evidence for the presence of two different β-globin chains in the hemoglobin of the river buffalo (Bubalus bubalis L.) | |
| US6833060B2 (en) | Electrophoresis gel support | |
| Webb | Acetylcholinesterase: characterization of native and proteolytically derived forms and identification of structural protein components | |
| Noordegraaf | Electrophoresis of glucosaminoglycans on cellulose acetate membranes with cation electrolytes: an electrofocusing phenomenon | |
| Kudo et al. | Ion-transfer polarographic study on the sodium-ion selectivity of bis (12-crown-4) at a water/nitrobenzene solution interface | |
| Pho et al. | The effect of 2-mercaptoethanol (ME) and edta on the sub-species structure of horse liver alcohol dehydrogenase (LADH) in 8 M urea | |
| SU662337A1 (ru) | Состав дл пропитки абразивного инструмента | |
| SUEHIRO et al. | STUDIES ON LIPOPROTEIN LIPASE I. DETERMINATION OF LIPOPROTEIN LIPASE | |
| SU883142A1 (ru) | Раствор дл травлени шлифов |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAL | Patent in force |
Ref document number: 8205386-9 Format of ref document f/p: F |