RU99112590A - METHOD FOR PRODUCING LIQUID OR DRY BACTERIAL FERTILIZER FOR THE PRODUCTION OF ACCUMULATED PRODUCTS - Google Patents

METHOD FOR PRODUCING LIQUID OR DRY BACTERIAL FERTILIZER FOR THE PRODUCTION OF ACCUMULATED PRODUCTS

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Publication number
RU99112590A
RU99112590A RU99112590/13A RU99112590A RU99112590A RU 99112590 A RU99112590 A RU 99112590A RU 99112590/13 A RU99112590/13 A RU 99112590/13A RU 99112590 A RU99112590 A RU 99112590A RU 99112590 A RU99112590 A RU 99112590A
Authority
RU
Russia
Prior art keywords
yeast
growth medium
milk
uterine
concentration
Prior art date
Application number
RU99112590/13A
Other languages
Russian (ru)
Other versions
RU2171585C2 (en
Inventor
Таисия Ивановна Анисимова
Анатолий Алексеевич Кустов
Original Assignee
Таисия Ивановна Анисимова
Filing date
Publication date
Application filed by Таисия Ивановна Анисимова filed Critical Таисия Ивановна Анисимова
Priority to RU99112590A priority Critical patent/RU2171585C2/en
Priority claimed from RU99112590A external-priority patent/RU2171585C2/en
Application granted granted Critical
Publication of RU2171585C2 publication Critical patent/RU2171585C2/en
Publication of RU99112590A publication Critical patent/RU99112590A/en

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Claims (4)

1. Способ получения жидкой или сухой бактериальной закваски для производства кисломолочных продуктов, включающий культивирование Lactobacillus acidophilus на ростовой питательной среде, содержащей обезжиренное молоко и водную суспензию 20%-ного кукурузного экстракта с получением маточной закваски, внесение ее в стерилизованное охлажденное до температуры заквашивания ростовую среду, содержащую молоко и лимоннокислый натрий, культивирование, охлаждение и выдержку с последующей фасовкой жидкой закваски или смешиванием последней с защитной средой, замораживанием и сушкой, отличающийся тем, что для получения маточной закваски из Lactobacillus acidophilus используют штамм Lactobacillus acidophilus n.v. 317/402 "Наринэ" ААА, в составе ростовой питательной среды дополнительно используют селенит натрия в концентрации (1,25-1,6)•10-6 мас. %, причем обезжиренное молоко используют с содержанием сухих веществ 9-13% при следующем соотношении компонентов, мас.%:
Водная суспензия 20%-ного кукурузного экстракта - 1,5-5,0
Селенит натрия - 1,25-1,6•10-6
Обезжиренное молоко с содержанием сухих веществ 9-13% - Остальное
Для культивирования маточной закваски в составе ростовой среды молоко используют с содержанием сухих веществ 14-18%, концентрация лимоннокислого натрия в молоке составляет (0,3-0,6)•10-2 мас.%, культивирование осуществляют до конечного титра живых клеток бактерий (4,0-8,0)•109 в 1 мл жидкой закваски.1. A method of obtaining a liquid or dry bacterial starter culture for the production of fermented milk products, comprising cultivating Lactobacillus acidophilus on a growth medium containing skim milk and an aqueous suspension of 20% corn extract to obtain a mother yeast, adding it to a sterilized growth medium cooled to the fermentation temperature , containing milk and sodium citrate, cultivation, cooling and aging, followed by packing liquid yeast or mixing the latter with a protective medium, freezing and drying, characterized in that the strain Lactobacillus acidophilus nv 317/402 "Narine" AAA is used to obtain uterine yeast from Lactobacillus acidophilus, and sodium selenite in a concentration of (1.25-1.6) • 10 is additionally used in the growth medium -6 wt. %, and skim milk is used with a solids content of 9-13% in the following ratio of components, wt.%:
Aqueous suspension of 20% corn extract - 1.5-5.0
Sodium Selenite - 1.25-1.6 • 10 -6
Skim milk with a solids content of 9-13% - Else
For the cultivation of uterine yeast in the growth medium, milk is used with a solids content of 14-18%, the concentration of sodium citrate in milk is (0.3-0.6) • 10 -2 wt.%, Cultivation is carried out to the final titer of living bacteria cells (4.0-8.0) • 10 9 in 1 ml of liquid starter culture. 2. Способ по п.1, отличающийся тем, в составе ростовой питательной среды для получения маточной закваски дополнительно используют аскорбиновую кислоту в концентрации (4,0-5,5)•10-2 мас.%.2. The method according to claim 1, characterized in that, in the composition of the growth medium, ascorbic acid at a concentration of (4.0-5.5) • 10 -2 wt.% Is additionally used to obtain a uterine yeast. 3. Способ по п.1, отличающийся тем, в составе ростовой питательной среды для получения маточной закваски дополнительно используют аминокислоту α-цистин в концентрации (1,0-2,5)•10-2 мас.%.3. The method according to claim 1, characterized in that in the composition of the growth medium for the production of uterine yeast, the amino acid α-cystine is additionally used in a concentration of (1.0-2.5) • 10 -2 wt.%. 4. Способ по п.1, отличающийся тем, в составе ростовой питательной среды для получения маточной закваски дополнительно используют смесь компонентов, указанных в пп.2 и 3. 4. The method according to claim 1, characterized in that in the composition of the growth nutrient medium to obtain a uterine sourdough, a mixture of the components specified in claims 2 and 3 is additionally used.
RU99112590A 1999-06-22 1999-06-22 Method of preparing liquid or dry bacterial ferment for fermented-milk foodstuffs production RU2171585C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU99112590A RU2171585C2 (en) 1999-06-22 1999-06-22 Method of preparing liquid or dry bacterial ferment for fermented-milk foodstuffs production

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU99112590A RU2171585C2 (en) 1999-06-22 1999-06-22 Method of preparing liquid or dry bacterial ferment for fermented-milk foodstuffs production

Publications (2)

Publication Number Publication Date
RU2171585C2 RU2171585C2 (en) 2001-08-10
RU99112590A true RU99112590A (en) 2001-11-10

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Family Applications (1)

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RU99112590A RU2171585C2 (en) 1999-06-22 1999-06-22 Method of preparing liquid or dry bacterial ferment for fermented-milk foodstuffs production

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2607023C1 (en) * 2015-10-22 2017-01-10 Общество с ограниченной ответственностью "Зеленые линии" Dry bacterial starter for production of fermented milk products and production method thereof

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