RU94005709A - BACILLUS SCHLEGELII BACTERIA STRAIN - PRODUCER OF ENDONUCLEASE RESTRICTION, LEARNING AND SPLITTING SEQUENCE OF NUCLEOTIDES 5'-CCNNNNNNNGG-3 ' - Google Patents

BACILLUS SCHLEGELII BACTERIA STRAIN - PRODUCER OF ENDONUCLEASE RESTRICTION, LEARNING AND SPLITTING SEQUENCE OF NUCLEOTIDES 5'-CCNNNNNNNGG-3 '

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Publication number
RU94005709A
RU94005709A RU94005709/13A RU94005709A RU94005709A RU 94005709 A RU94005709 A RU 94005709A RU 94005709/13 A RU94005709/13 A RU 94005709/13A RU 94005709 A RU94005709 A RU 94005709A RU 94005709 A RU94005709 A RU 94005709A
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RU
Russia
Prior art keywords
ccnnnnnnngg
restrictase
producer
nucleotides
learning
Prior art date
Application number
RU94005709/13A
Other languages
Russian (ru)
Other versions
RU2073717C1 (en
Inventor
В.Е. Репин
Т.А. Терещенко
Л.Р. Лебедев
Original Assignee
Товарищество с ограниченной ответственностью "Сиэнзим"
Filing date
Publication date
Application filed by Товарищество с ограниченной ответственностью "Сиэнзим" filed Critical Товарищество с ограниченной ответственностью "Сиэнзим"
Priority to RU94005709A priority Critical patent/RU2073717C1/en
Priority claimed from RU94005709A external-priority patent/RU2073717C1/en
Publication of RU94005709A publication Critical patent/RU94005709A/en
Application granted granted Critical
Publication of RU2073717C1 publication Critical patent/RU2073717C1/en

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Claims (1)

Применение: микробиология и биотехнология. Техническая задача: выявление штамма-продуцента, обеспечивающего получение термостабильной рестриктазы, узнающей и расщепляющей нуклеотидную последовательность 5'-CCNNNNNNNGG-3' с высоким выходом и активностью фермента. Сущность изобретения: предложен новый штамм Bacillus schlegelii (ВКПМ В-8310) - продуцент рестриктазы Bso4 1, выделенный из почвы в результате поиска продуцентов рестриктаза. Положительный эффект: выход фермента составляет 5 000 ед/г сырой биомассы, удельная активность целевого фермента - 10000 млн- 1. Рестриктаза Bso4 1 является изошизомером рестриктазы Bsi Y 1 и может заменить последнюю во всех генно-инженерных работах. 1 ил. 2 прим.Application: microbiology and biotechnology. Technical task: identification of a producer strain that provides a thermostable restrictase that recognizes and cleaves the nucleotide sequence of the 5'-CCNNNNNNNGG-3 'in high yield and enzyme activity. The inventive proposed new strain of Bacillus schlegelii (VKPM B-8310) - producing restrictase Bso4 1, isolated from the soil as a result of the search for producers of restrictase. Positive effect: enzyme yield was 5000 units / g wet biomass, the specific activity of the target enzyme - 10000 million - 1. Bso4 1 restriction enzyme is the isoschisomer of Bsi Y 1 restrictase and can replace the latter in all genetic engineering works. 1 il. 2 ca.
RU94005709A 1994-02-08 1994-02-08 Strain of bacterium bacillus schlegelii - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ccnnnnnnngg-3' RU2073717C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94005709A RU2073717C1 (en) 1994-02-08 1994-02-08 Strain of bacterium bacillus schlegelii - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ccnnnnnnngg-3'

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94005709A RU2073717C1 (en) 1994-02-08 1994-02-08 Strain of bacterium bacillus schlegelii - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ccnnnnnnngg-3'

Publications (2)

Publication Number Publication Date
RU94005709A true RU94005709A (en) 1995-10-20
RU2073717C1 RU2073717C1 (en) 1997-02-20

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU94005709A RU2073717C1 (en) 1994-02-08 1994-02-08 Strain of bacterium bacillus schlegelii - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ccnnnnnnngg-3'

Country Status (1)

Country Link
RU (1) RU2073717C1 (en)

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