RU93057108A - BACILLUS BADIUS BACTERIA STRAIN - PRODUCER OF ENDONUCLEASE RESTRICTION, LEARNING AND SPLITTING SEQUENCE OF NUCLEATIDES 5 '- (A / T) CCGG (A / T) -3' - Google Patents

BACILLUS BADIUS BACTERIA STRAIN - PRODUCER OF ENDONUCLEASE RESTRICTION, LEARNING AND SPLITTING SEQUENCE OF NUCLEATIDES 5 '- (A / T) CCGG (A / T) -3'

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Publication number
RU93057108A
RU93057108A RU93057108/13A RU93057108A RU93057108A RU 93057108 A RU93057108 A RU 93057108A RU 93057108/13 A RU93057108/13 A RU 93057108/13A RU 93057108 A RU93057108 A RU 93057108A RU 93057108 A RU93057108 A RU 93057108A
Authority
RU
Russia
Prior art keywords
ccgg
nucleatides
producer
learning
bacillus badius
Prior art date
Application number
RU93057108/13A
Other languages
Russian (ru)
Other versions
RU2053299C1 (en
Inventor
С.Х. Дегтярев
В.Е. Репин
Н.И. Речкунова
А.В. Шевченко
М.А. Абдурашитов
Original Assignee
Товарищество с ограниченной ответственностью "Сибэнзим"
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Publication date
Application filed by Товарищество с ограниченной ответственностью "Сибэнзим" filed Critical Товарищество с ограниченной ответственностью "Сибэнзим"
Priority to RU93057108A priority Critical patent/RU2053299C1/en
Priority claimed from RU93057108A external-priority patent/RU2053299C1/en
Application granted granted Critical
Publication of RU2053299C1 publication Critical patent/RU2053299C1/en
Publication of RU93057108A publication Critical patent/RU93057108A/en

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Claims (1)

Применение: микробиология и биотехнология. Техническая задача: выявление продуктивного штамма, обеспечивающего получение рестриктазы, узнающей и расщепляющей нуклеотидную последовательность 5'-(A/T)CCGG(A/T)-3', не требовательного к питательным средам и с небольшим временем культивирования. Сущность изобретения: предложен новый штамм Bacillus badius (ВКПМ-В-6616) - продуцент рестриктазы Bba179 I, выделенный из почвы в результате поиска продуцентов рестриктаз. Положительный эффект: выход фермента составляет 1000 ед/г сырой биомассы, удельная активность целевого фермента составляет 3000 ед/мл. Рестриктаза Bba179 I является изошизомером рестриктазы Bet I и может заменить последнюю во всех генно-инженерных работах.Application: microbiology and biotechnology. Technical task: the identification of a productive strain that provides restrictase, recognizes and cleaves the nucleotide sequence of 5 '- (A / T) CCGG (A / T) -3', not demanding on nutrient media and with a short cultivation time. The inventive proposed a new strain of Bacillus badius (VKPM-B-6616) - producing restrictase Bba179 I, isolated from the soil as a result of the search for producers of restricts. Positive effect: the output of the enzyme is 1000 U / g of raw biomass, the specific activity of the target enzyme is 3000 U / ml. The restriction enzyme Bba179 I is an isoschisomer of restriction enzymes Bet I and can replace the latter in all genetic engineering works.
RU93057108A 1993-12-22 1993-12-22 Strain of bacterium bacillus badius - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-(a/t)ccgg(a/t)-3′ RU2053299C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93057108A RU2053299C1 (en) 1993-12-22 1993-12-22 Strain of bacterium bacillus badius - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-(a/t)ccgg(a/t)-3′

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93057108A RU2053299C1 (en) 1993-12-22 1993-12-22 Strain of bacterium bacillus badius - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-(a/t)ccgg(a/t)-3′

Publications (2)

Publication Number Publication Date
RU2053299C1 RU2053299C1 (en) 1996-01-27
RU93057108A true RU93057108A (en) 1996-07-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU93057108A RU2053299C1 (en) 1993-12-22 1993-12-22 Strain of bacterium bacillus badius - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-(a/t)ccgg(a/t)-3′

Country Status (1)

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RU (1) RU2053299C1 (en)

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