RU93003626ARU93003626/13ARU93003626ARU93003626ARU 93003626 ARU93003626 ARU 93003626ARU 93003626/13 ARU93003626/13 ARU 93003626/13ARU 93003626 ARU93003626 ARU 93003626ARU 93003626 ARU93003626 ARU 93003626A
Штамм относится к микробиологии и биотехнологии. Изобретение позволяет выявить штамм-продуцент, обеспечивающий получение термостабильной рестриктазы, узнающей и расщепляющей нуклеотидную последовательность 5'-CT(A/G)(T/C)AG-3', не требовательный к питательным средам и условиям культивирования. Сущность изобретения: новый штамм Bacillus coagulans (ВКПМ-В6311) - продуцент рестриктазы Bco163 l, выделенный из термальных источников в результате поиска продуцентов рестриктаз. Выход фермента составляет 1000 ед/г сырой биомассы, удельная активность целевого фермента - 3000 ед/мл. Рестриктаза Bco163 l является изошизомером рестриктазы Bdi l и может заменить ее во всех генно-инженерных работах.The strain relates to microbiology and biotechnology. The invention allows to identify the producer strain, providing thermostable restrictase, recognizing and cleaving the nucleotide sequence of the 5'-CT (A / G) (T / C) AG-3 ', not demanding on nutrient media and culture conditions. The essence of the invention: a new strain of Bacillus coagulans (VKPM-B6311) - producing restrictase Bco163 l, isolated from thermal sources as a result of the search for producers of restricts. The enzyme yield is 1000 U / g of raw biomass, the specific activity of the target enzyme is 3000 U / ml. Restriction enzyme Bco163 l is an isoschisomer of Bdi l restriction enzymes and can replace it in all genetic engineering works.
RU93003626A1993-01-221993-01-22Strain of bacteria bacillus coagulants being producer of side specific endonuclease which is enable to identify and to cleave series of 5′-ct(a/g)(t/c)ag-3′ nucleotides
RU2040540C1
(en)
Strain of bacteria bacillus coagulants being producer of side specific endonuclease which is enable to identify and to cleave series of 5′-ct(a/g)(t/c)ag-3′ nucleotides
Strain of bacteria bacillus coagulants being producer of side specific endonuclease which is enable to identify and to cleave series of 5′-ct(a/g)(t/c)ag-3′ nucleotides
Strain of bacteria bacillus coagulants being producer of side specific endonuclease which is enable to identify and to cleave series of 5′-ct(a/g)(t/c)ag-3′ nucleotides
BACILLUS BADIUS BACTERIA STRAIN - PRODUCER OF ENDONUCLEASE RESTRICTION, LEARNING AND SPLITTING SEQUENCE OF NUCLEATIDES 5 '- (A / T) CCGG (A / T) -3'
Strain of bacteria bacillus coagulants being producer of side specific endonuclease which is enable to identify and to cleave series of 5′-ct(a/g)(t/c)ag-3′ nucleotides
Strain of bacterium bacillus species - a producer of restriction endonuclease recognizing and cleaving nucleotide sequence 5'-(a/g)-ccgg-(t/c)-3'
Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-gg(t/g)(ag)cc-3'
FOERFARANDE FOER FRAMSTAELLNING AV EN GENETISKT MANIPULERAD MICROORGANISM INNEHAOLLANDE EN FOER AMYLAS KODANDE REKOMBINANT-DNA, OCH ETT FOERFARANDE FOER FRAMSTAELLNING AV AMYLAS.