RU93015542A -
STRAIN OF ACINETOBACTER CALCOACETICUS BACTERIA - PRODUCER OF RESTRUCTION ENDONUCLEASE, RECOGNIZING AND DECLINING 5 'NUCLEOTIDE SEQUENCE - GGTACC-3'
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STRAIN OF ACINETOBACTER CALCOACETICUS BACTERIA - PRODUCER OF RESTRUCTION ENDONUCLEASE, RECOGNIZING AND DECLINING 5 'NUCLEOTIDE SEQUENCE - GGTACC-3'
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RU93015542A
RU93015542ARU93015542/13ARU93015542ARU93015542ARU 93015542 ARU93015542 ARU 93015542ARU 93015542/13 ARU93015542/13 ARU 93015542/13ARU 93015542 ARU93015542 ARU 93015542ARU 93015542 ARU93015542 ARU 93015542A
Применение: микробиология и биотехнология. Цель: выявление штамма-продуцента, непатогенного и обеспечивающего получение рестриктазы, узнающей и расщепляющей нуклеотидную последовательность 5'-GGTACC-3', с высоким выходом и активностью фермента. Сущность изобретения: предложен новый штамм Acinetobacter calcoaceticus (ВКПМ-В6337) - продуцент рестриктазы Асс651, выделенный в результате поиска продуцентов рестриктаз. Положительный эффект: выход фермента составляет 60000 ед/г сырой биомассы, удельная активность целевого фермента составляет 10000-100000 ед/мл. Рестриктаза Асс651 является изошизомером рестриктазы kpn 1 и может заменить последнюю во всех генно-инженерных работах.Application: microbiology and biotechnology. Purpose: to identify a producer strain that is non-pathogenic and provides a restriction enzyme that recognizes and cleaves the nucleotide sequence of 5'-GGTACC-3 ', in high yield and enzyme activity. The inventive proposed a new strain of Acinetobacter calcoaceticus (VKPM-B6337) - producer of the restriction enzyme Ass651, isolated by searching for producers of restrictase enzymes. Positive effect: the yield of the enzyme is 60,000 units / g of crude biomass, the specific activity of the target enzyme is 10,000-100,000 units / ml. The restriction enzyme Ass651 is an isoschisomer of the restriction enzyme kpn 1 and can replace the latter in all genetic engineering activities.
RU93015542A1993-03-241993-03-24Strain of bacterium acinetobacter calcoaceticus - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ggtacc-3'
RU2034922C1
(en)
Strain of bacterium acinetobacter calcoaceticus - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ggtacc-3'
Strain of bacterium acinetobacter calcoaceticus - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ggtacc-3'
Strain of bacterium acinetobacter calcoaceticus - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-ggtacc-3'
Strain of bacterium bacillus coagulants - a producer of restriction enduclease that recognizes and cleaves the nucleotide sequence 5'-gatnnnnatc-3'
Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-gg(t/g)(ag)cc-3'
BACILLUS BADIUS BACTERIA STRAIN - PRODUCER OF ENDONUCLEASE RESTRICTION, LEARNING AND SPLITTING SEQUENCE OF NUCLEATIDES 5 '- (A / T) CCGG (A / T) -3'