RU94017996A - Strain of bacterium bacillus coagulants - a producer of restriction enduclease that recognizes and cleaves the nucleotide sequence 5'-gatnnnnatc-3' - Google Patents

Strain of bacterium bacillus coagulants - a producer of restriction enduclease that recognizes and cleaves the nucleotide sequence 5'-gatnnnnatc-3'

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Publication number
RU94017996A
RU94017996A RU94017996/13A RU94017996A RU94017996A RU 94017996 A RU94017996 A RU 94017996A RU 94017996/13 A RU94017996/13 A RU 94017996/13A RU 94017996 A RU94017996 A RU 94017996A RU 94017996 A RU94017996 A RU 94017996A
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RU
Russia
Prior art keywords
enzyme
producer
strain
gatnnnnatc
cleaves
Prior art date
Application number
RU94017996/13A
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Russian (ru)
Other versions
RU2057806C1 (en
Inventor
В.Е. Репин
Т.А. Терещенко
Л.Р. Лебедев
Original Assignee
Товарищество с ограниченной ответственностью "Сибэнзим"
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Application filed by Товарищество с ограниченной ответственностью "Сибэнзим" filed Critical Товарищество с ограниченной ответственностью "Сибэнзим"
Priority to RU94017996A priority Critical patent/RU2057806C1/en
Application granted granted Critical
Publication of RU2057806C1 publication Critical patent/RU2057806C1/en
Publication of RU94017996A publication Critical patent/RU94017996A/en

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

FIELD: microbiology, biotechnology, genetic engineering. SUBSTANCE: new strain B. COAGULANTS ("ВКПМ" B-6670) - a producer of restrictase Bco631 I isolated from thermal source as a result of restrictase screening. Restrictase Bco631 I is an isoschizomer of enzyme BsaB I and can replace the latter in all genetic-engineering studies. Enzyme yield is 6000 U/g wet biomass, specific activity is 10000 U/ml. EFFECT: high yield and activity of enzyme. 1 dwg, 2 notes

Claims (1)

Применение: микробиология и биотехнология. Цель - выявление штамма-продуцента, обеспечивающего получение термостабильной рестриктазы, узнающей и расщепляющей нуклеотидную последовательность 5'-GATNNNNATC-3', с высоким выходом и активностью фермента. Сущность : Предложен новый штамм Bacillus coagulans (ВКПМ B-6670) - продуцент рестриктазы Вcо631 1, выделенный из термальных источников в результате поиска продуцентов рестриктаз. Положительный эффект: Выход фермента 6000 ед/г сырой биомассы, удельная активность целевого фермента 1000 ед/мл. Рестриктаза Всo631 1 является изошизомером рестриктазы BsaB 1 и может заменить последнюю во всех генно-инжнерных работах.Application: microbiology and biotechnology. The goal is to identify a producer strain that provides thermostable restriction enzyme that recognizes and cleaves the nucleotide sequence of 5'-GATNNNNATC-3 ', in high yield and enzyme activity. Essence: A new strain of Bacillus coagulans (VKPM B-6670) is proposed - a producer of restriction enzyme Bco631 1 isolated from thermal sources as a result of a search for producers of restrictase enzymes. Positive effect: The output of the enzyme is 6000 units / g of crude biomass, the specific activity of the target enzyme is 1000 units / ml. The restriction enzyme Bso631 1 is an isoschisomer of the restriction enzyme BsaB 1 and can replace the latter in all genetic engineering studies.
RU94017996A 1994-05-17 1994-05-17 Strain of bacterium bacillus coagulans - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-gatnnnn-atc-3′ RU2057806C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94017996A RU2057806C1 (en) 1994-05-17 1994-05-17 Strain of bacterium bacillus coagulans - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-gatnnnn-atc-3′

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94017996A RU2057806C1 (en) 1994-05-17 1994-05-17 Strain of bacterium bacillus coagulans - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-gatnnnn-atc-3′

Publications (2)

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RU2057806C1 RU2057806C1 (en) 1996-04-10
RU94017996A true RU94017996A (en) 1996-08-20

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RU94017996A RU2057806C1 (en) 1994-05-17 1994-05-17 Strain of bacterium bacillus coagulans - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5′-gatnnnn-atc-3′

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2475533C1 (en) * 2011-12-12 2013-02-20 Общество с ограниченной ответственностью "СибЭнзайм" BACTERIA STRAIN Microbacterium testaceum 17B-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE MteI
US9596861B2 (en) * 2013-12-24 2017-03-21 Sami Labs Limited Method of producing partially purified extracellular metabolite products from Bacillus coagulans and biological applications thereof

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RU2057806C1 (en) 1996-04-10

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