RU2696440C1 - Method for assessment of soil toxicity reduction when introducing whey or manure - Google Patents

Abstract

FIELD: agriculture.SUBSTANCE: invention relates to agriculture and soil science, namely to a method of assessing soil toxicity for increasing yield of grain crops. Assessment of soil toxicity is carried out by value of inhibition (I1) development of seeds of grain crops during their sowing on this soil and data on assessment of toxicosis of soil treated with serum or manure, according to value of inhibition of seed development on soil treated with ameliorant (I2). Value of I1 indicates the need to treat soil with such additives as milk whey or manure. If I1 40 % is exceeded, necessity of soil treatment with reclamation agent is determined to produce stable harvests of grain crops. Evaluation of the reclamation agent use efficiency is determined by the inhibition value decrease size ΔI, calculated by determining difference between I1 and I2.EFFECT: invention provides reducing toxicity of allelotoxins of grey forest soil by using reclaiming additives – milk whey or manure; in particular, treatment of soil with milk whey or manure reduces inhibition of development of seeds by 18 % and 12 % respectively; wherein use of milk whey is 1½ times more effective compared to the use of manure.7 cl, 1 tbl, 1 ex

Description

Technical field

The invention relates to agriculture and soil science, and in particular to methods that identify drugs to increase productivity by reducing the amount of allelotoxins in soils (soil toxicosis) by adding various substances to soils and assessing the effect of their application on the degree of soil fatigue.

In the modern scientific literature, reliable information has been accumulated that as a result of repeated cultivation of plants of the same species in the same field (monoculture), as well as a number of other reasons, progressive loss of fertility occurs. This is primarily associated with increasing toxicosis of the fertile layer, due to the accumulation of toxic emissions of plants (colins) and microorganisms in it, which can be represented by various compounds and their mixtures. Moreover, some of the components of toxic mixtures may not have toxicity, toxic compounds may be in concentrations when each of them individually is not toxic to plants. This is the cause of soil fatigue and lower crop yields.

The proposed method for assessing the reduction of soil toxicosis (allelopathic soil fatigue) will allow you to choose the most effective additives that increase the yield of crops.

State of the art

Various methods for a similar purpose are known in the art. In particular, methods are known for assessing the reduction of soil toxicosis, when toxicosis was reduced by washing the soil with large amounts of water, or by heating and drying the soil [1]. Toxicosis reduction was assessed by comparing crop yields in vegetation and field experiments. The main disadvantage of this method is the irreproducibility of the results and the absence in many cases of the effect of its use.

A known method for determining the allelopathic activity of soils, which includes two stages and involves the release of toxins from the soil by chemical displacement by rutin, followed by seed germination on toxin solutions and taking into account their laboratory germination [2].

This method is expensive, time-consuming, time-consuming and difficult to implement, and therefore requires high qualification of performers and does not find wide practical use. In addition, the chemical method of extracting toxins from the soil does not ensure their complete release into the solution, which reduces the objectivity of the method and affects the reliability of the results.

Closest to the claimed is a method for assessing the reduction of soil toxicosis [3], including the germination of seeds in a test soil placed in vegetation vessels or cuvettes, while the germination is carried out for 10-12 days after 20 minutes of soaking in warm (30 ° C) water and two hours of drying on filter paper in soil tested for soil fatigue, selected in a root-inhabited layer of 0-20 cm at a moisture content of 60% of full moisture capacity, 12-hour (per day) lighting of 5 thousand lk As an assessment test, a mixture of three test cultures of radish, lettuce, and meadow timothy is used, followed by calculation of the germination percentage, on the basis of which three degrees of soil fatigue are distinguished: low (germination is 76% and higher); medium (from 50 to 75%); high (49% and below), based on a comparison of the germination rate of treated and untreated soil additives, they conclude that the effectiveness of the application of substances that reduce soil toxicosis and the feasibility of use in agricultural production.

The disadvantages of this method are its low productivity due to the duration of the experiment and the obtaining of average results on test cultures.

Disclosure of invention

The technical problem solved by the claimed invention is the need to overcome the disadvantages inherent in analogues, namely, the need to create a method for assessing the reduction of soil toxicosis when certain additives are added to the soil (in particular whey or manure), which consists in assessing the inhibition of the development of seedlings in the soil (%) and identifying the effectiveness of the added additives to reduce soil toxicosis, by assessing the inhibition of seedling development in the treated soil and determining the size of the decrease and inhibiting the development of seedlings when these additives are added to the soil.

The method allows to identify an additive (ameliorating preparation) that significantly reduces the inhibition of the development of grain seeds by alleotoxins, taking into account the contribution of the negative effect of soils on seeds developing in soils, which can avoid inhibition of the development of seeds and developing from them in plant soils, up to a significant yield loss.

The technical result achieved by using the claimed invention is to provide the ability to test the suitability of ameliorating additives, in particular whey and manure, for cultivating the soil in order to reduce soil toxicosis. In addition, the technical result consists in increasing the productivity of the method while providing the possibility of simultaneous measurements both for large batches of seeds (from 1000 to 1200 seeds), and for a wide range of reclamation preparations and soils, which leads to an increase in the reliability of the results due to the possibility of increasing the size of the statistical sample. The result for thousands of seeds can be obtained after 48 hours. The increase in the number of seeds practically does not complicate the work, since as a result of the implementation of the proposed method, an independent integral parameter (namely, the bulk volume of sprouted seeds) is determined, which significantly increases the accuracy of the results. In connection with the use of 1200 seeds in one experiment (6 replicates of 200 seeds each), the error associated with the different quality of seeds is minimized. The use of six-fold repetition followed by statistical processing of the results leads to the fact that according to the Student criterion, the error at a 95% significance level does not exceed 7%.

The problem is solved in that the method for assessing the reduction of soil toxicosis, according to the technical solution, includes the following steps that are carried out sequentially:

- the formation of at least two soil samples with toxicosis and one sand sample,

- treatment of one soil sample with ameliorating additive, which is used as whey or manure,

- the formation of at least three weighed seeds of grain crops,

- obtaining a sample of seeds in the sand by ensuring contact of the seed sample with sand with the addition of water to achieve the lowest moisture capacity of the sand;

- obtaining a sample of seeds in the soil with toxicosis, by ensuring the contact of one weighed seed with this soil, with the addition of water to achieve the lowest soil moisture capacity;

- obtaining a seed sample in the soil treated with the reclamation additive, by ensuring that another portion of the seeds comes into contact with the soil treated with the reclamation additive, with the addition of water until the soil has the lowest moisture capacity;

- exposure of the samples to germination,

- removal of soil and sand from samples and placement of purified germinated seeds of different samples in identical transparent containers with water,

- compaction of germinated seeds in containers by means of vibration in the vertical plane, and subsequent impact on the bottom of the container, and after vibration exposure to seed samples in the container, loads of identical mass are placed,

- determination of the height of cargo placement from the bottom of the tank of bulk volumes of germinated seeds from a sample with sand (V1), from a sample with soil having toxicosis (V2), and from a sample with soil treated with whey or manure (V3),

- determination of the amount of inhibition of seed development for a sample with soil with toxicosis (I1), which is used to judge the degree of toxicosis of the soil, according to the formula:

I1 = ((V1-V2) / (V1-20)) * 100%. - determination of the amount of inhibition of seed development for a sample with treated soil (I2), which is used to judge the degree of toxicosis of the treated soil, according to the formula:

And2 = ((V1-V3) / (V1-20)) * 100%.

- determination of the magnitude of the change in inhibition as the difference between the inhibition of seed development in the soil with toxicosis (I1) and the inhibition of seed development in the treated soil reclamation additive (I2), which is used to judge the degree of toxicosis of the treated soil reclamation additive, according to the formula: ΔI = I1- AND 2,

at the same time, the degree of inhibition (ΔI) is used to judge the degree of reduction of soil toxicosis when whey or manure is added.

Extract of seeds before germination is carried out at a temperature of 22-25 ° C at 100% humidity for 2 days. The placement and compaction of seeds in the container is metered for volumes not exceeding 1 / 5-1 / 4 of the volume of germinated seeds from the sample. The method is performed in six replicates for all seed samples in sand, soil with toxicosis, and soil treated with whey or manure. Vibration exposure in the vertical plane is carried out with a frequency of not more than 50 Hz and an amplitude of 3-4 mm with an allowable deviation from the indicated values of not more than 10%. When impacted, it is provided with a force equal to the force of free fall of a cylindrical container filled with water and seeds on the table surface from a height of 1 cm with a permissible deviation from the indicated values of not more than 10%. as identical transparent containers use transparent cylinders with a ratio of height and diameter of 5: 8.

As a result of the implementation of the proposed method, reliable data are obtained on the assessment of soil toxicosis by the amount of inhibition (I1) of the development of seeds of grain crops when they are sown on this soil and data on the assessment of toxicosis of the soil treated with serum, by the amount of inhibition of the development of seeds on the soil treated with ameliorant (I2 ) At the same time, by the amount of inhibition (I1), the need for soil treatment with such additives as whey or manure is judged. If I1 is exceeded 40%, it is necessary to cultivate the soil with a meliorative preparation to obtain stable crops of grain crops. Evaluation of the effectiveness of using the reclamation preparation is determined by the size of the decrease in the amount of inhibition (ΔI), calculated by determining the difference between I1 and I2. The greater the ΔI, the more effective the reclamation additive is to reduce soil toxicosis.

At the same time, it is considered effective to reduce soil toxicosis with an I2 value of not more than 40%.

The technical essence of the invention lies in the fact that the reaction of seeds to a decrease in soil inhibition is determined by their ability to germinate and give roots and sprouts (seedlings) during the development of seeds on soils treated with ameliorating preparations (whey) compared with the development of seeds on soils not treated with ameliorating preparations. Soils, as a rule, contain toxins formed by phytopathogens that functioned in them, upon decomposition of plant residues or secreted by plants. When bringing seeds into contact with moist soil, the flow of toxins from the soil into the seeds begins. It should be noted that whey contains a large number of lactic acid bacteria, which use allelotoxins accumulated in the soil for nutrition, reducing their concentration in the soil and, accordingly, soil toxicosis. As a result, the seeds will germinate better on those soils whose cultivation has led to a decrease in soil toxicosis, and the plants formed from them will develop better in these soils and yield maximum yields.

The toxin content in sand compared to soil is negligible, which allows us to take the development of seedlings in the sand as a base indicator for comparison. This is also possible because seeds germinating within 2 days do not yet begin to absorb nutrients from the soil, but develop due to accumulated resources. When water is added to the soil, the toxins pass into the solution and affect the seeds already in the first two days, and the amount of toxins decreases when the soil is treated with milk whey. Therefore, when comparing the development in the soil with the development of seeds in the sand, one can evaluate the reaction of seeds to toxins contained in the soil and determine the toxicosis of soils by the degree of inhibition of seed germination by the soil, compared to sand (I1). When comparing the development of seeds in soil treated with milk whey with the development of seeds in sand, one can evaluate the reaction of seeds to toxins contained in this soil and determine the toxicosis of the treated soils by the degree of inhibition of seed germination by the treated soil compared to sand (I2). Due to the fact that the first stages of germination (swelling and hatching of seeds) are caused by substances stored during seed ripening, information on the passage of biochemical processes in seeds can be obtained only at the stage of seedling development, that is, approximately 24-36 hours after the seeds are brought in contact with moisture at a temperature of 22-25 ° C. The difference in the inhibition of seed development in soil that was initially toxic and the inhibition of seed development in soil treated with ameliorating additive determines the decrease in the inhibition value (ΔI). By assessing the size of the decrease in the magnitude of inhibition, a decrease in soil toxicosis is judged when whey or manure is taken, taken as ameliorating additives.

To do this, the same weighed seeds are placed in containers, some of which contain sand, respectively, and other soil samples with toxicosis and treated serum. They are supplemented with water samples that provide optimal seed development in the soil and sand. After this, the containers with samples are thermostated at an air humidity close to 100%. After about two days (40-50 hours), the bulk volumes of seeds sprouted in the soil and sand are measured and the ratio of the bulk volumes in the sand and the studied soils is compared, respectively, determining the amount of inhibition in percent germination of seeds in each soil sample compared to seeds in sand (I1, I2). It should be noted that due to the variability inherent in seeds, a comparison can be made by determining bulk volumes for large (more than 1000 pieces) seed arrays used in the experiment. Otherwise, it is not possible to detect significant differences in seed germination. Bulk volumes of sprouted seeds are determined in a cylinder with water with a volume of 100 ml, pouring into a cylinder with water, standing on a vibrating table, seeds with seedlings in small portions so that they settle separately from each other until a uniform structure (without voids) is formed from germinated seeds with a volume of about 20 ml Compaction is carried out to avoid the creation of large pores, which lead to an increase in error. Then, a load is placed on the seeds, compacting the structure of seeds with seedlings, this is done several times until all seeds with seedlings of the studied sample (7.5 g of the original seeds) are in the cylinder, after which additional densification of the formed structure of seeds with seedlings is carried out tapping the cylinder with seeds with seedlings and a small weight on their surface on the table, followed by measuring the bulk volume of the formed structure.

The difference in the determined values of inhibition of seed development in the soil that was initially toxicosis and inhibition of seed development in the soil treated with ameliorating additive determines the decrease in the inhibition value (ΔI), which corresponds to a decrease in soil toxicosis when treated with this ameliorating preparation.

Whey contains a large number of lactic acid bacteria, which use allelotoxins accumulated in the soil for nutrition, reducing their concentration in the soil and, accordingly, toxicosis of the soil.

The proposed method allows to evaluate the degree of influence of whey and manure on soil toxicosis. Confirmation of the effectiveness of whey in terms of reducing soil toxicosis allows us to recommend its use to increase soil fertility near dairies, where whey is a waste.

The implementation of the invention

An assessment of the reduction of soil toxicosis when whey is applied is made by detecting the presence of soil toxicosis during inhibition of seedling development by this soil above 40% and determining the difference in the values of the inhibition of seed germination in untreated and treated with ameliorating additives soil.

Soil toxicosis (inhibition value (I1)) is determined by the length of seedlings grown for 2 days in the original soil, compared with seedlings grown in sand, expressing toxicosis as a percentage of inhibition of seedling development.

Evaluation of the effectiveness of the introduction of such additives in terms of reducing soil toxicosis by the claimed method can be carried out using cereal grain crops such as wheat, rye, barley, triticale, etc., with a fibrous root system.

When forming a seed sample with untreated soil (soil with toxicosis) take a sample of air-dry soil. To form a seed sample in the soil treated with the ameliorating additive, the soil is pretreated as follows: the soil is treated with serum by adding 80 g of serum to 800 g of soil and after thorough mixing it is left for 7 days. The soil treatment with manure is carried out by adding to 800 g of soil a suspension containing 24 g of manure in 80 g of water, and after thorough mixing, leave for 7 days. After that, the treated soil samples are dried to a moisture content of 21.6% and tests are carried out.

For seed germination, any containers used for these purposes are used. Any soils of the zonal row can be used as substrates for germination: sod-podzolic, gray forest, chernozems, chestnut soils, and sand experience is used as a control in determining the toxicosis of the studied soil. The amount of substrate used for germination does not matter. A prerequisite is the same amount of substrate for germinating weighed seeds when forming samples with untreated soil, soil treated with serum (cattle manure) and sand samples. The same sample of seeds is placed in containers, covered with soil and sand, respectively, and water is added.

After this, the containers with germinated seeds are placed in a thermostatically controlled cabinet (at 22 ° C), in which an atmosphere of 100% humidity is created and kept for 2 days. After the specified time, the germinated seeds are washed from the soil and sand on a sieve and placed in graduated cylinders with water. The seeds are poured gradually so that they settle separately in the cylinder, while they exert a vibrational effect on the cylinder, forcing it to oscillate with a frequency of 50 Hz in a vertical plane with an amplitude of 3-4 mm (on a special vibrating table), facilitating the movement of seeds relative to each other when their seedlings will come in contact. Seeds with seedlings are placed in the cylinder in small portions until a uniform structure (without visible voids) of about 20 ml is formed, then a load weighing 8 g is placed on the seeds, compacting the seed structure, do this several times until all seeds with seedlings of the sample under study (7 , 5 g of the original seeds) will not be in the cylinder, after which the germinated seeds are compacted in the cylinder by lightly tapping the cylinder with the germinated seeds on the table (30-40 strokes), first placing a small weight on the surface of the seeds (based on pressure load 1-2 g / cm ²⁾ to create additional pressure on the surface of the seed in the cylinder. Then, the bulk volume of the germinated seeds in the cylinder is determined by the previously known value of the diameter of the cylinder and the height determined from the bottom of the cylinder to the surface of the load. Assessment of toxicosis reduction is carried out by the difference in the values of inhibition of seed germination in untreated and treated soil.

Inhibition by untreated soil (I1) is defined as a percentage as the ratio of the difference between the bulk volumes of seeds sprouted in sand and untreated soil, to the difference between the bulk volume of a sample of seeds sprouted in sand and a correction factor (20 ml). The inhibition of soil treated with serum (manure) (I2) is defined as a percentage as the ratio of the difference between the bulk volumes of seeds sprouted in the sand and the soil treated with serum (manure), the difference between the bulk volume of the sample of seeds sprouted in the sand and the correction factor (20 ml).

To measure the bulk volume as a transparent container (vessel) using transparent cylindrical containers with a ratio of height and diameter of the cylinder 5: 8. It is preferable to use laboratory graduated cylinders of transparent material.

The volume of water previously poured into the cylindrical container exceeds the volume of the measured seeds by 3-5 times and, when the sprouted seeds are gradually fallen into the cylinder, it provides separate settling of seeds with preservation of seedlings.

The impact is carried out by shaking the container with water and seeds 30-40 times with a force equal to the force of free fall of the cylinder on the table surface from a height of 1 cm with a permissible deviation from the indicated values of not more than 10%, while the weight of the weight should be less than the cylinder diameter by 2 mm (with a permissible deviation from the specified value of not more than 10%).

Below is a more detailed description of the proposed method, which does not limit the scope of claims of the claimed invention, but demonstrates the possibility of carrying out the invention with the achievement of the claimed technical result.

Example 1

During the tests, soil samples were used from the Tula region (Shchekino district) with a moisture content of 21.6% (after cereals), spring barley cultivar Nur, fresh cattle manure and milk whey.

Soil toxicosis (inhibition value (I1)) was determined by the length of seedlings grown for 2 days in the original soil, in comparison with seedlings grown in sand, expressing toxicosis as a percentage of inhibition of seedling development. The amount of inhibition (I1) was determined by the formula:

I1 = ((V1-V2) / (V1-20)) * 100%.

where 20 is a correction factor characterizing the bulk volume of swollen seeds (ml), the germination of which was carried out within 24 hours;

V1 - bulk volume of seeds germinated in the sand;

V2 - bulk volume of seeds germinated in the soil with toxicosis.

The value of soil toxicosis (I2) when treated with milk whey (manure) was determined by the length of seedlings grown in 2 days in soil treated with milk whey (manure), in comparison with seedlings grown in sand, expressing toxicosis as a percentage of inhibition of the development of seedlings. The amount of inhibition (I2) was determined by the formula:

I2 = ((V1-V3) / (V1-20)) * 100%

where 20 is a correction factor characterizing the bulk volume of swollen seeds (ml), the germination of which was carried out within 24 hours;

V1 - bulk volume of seeds germinated in the sand;

V3 - bulk volume sprouted in soil treated with whey.

The length of the seedlings was determined in accordance with the technique based on the existence of a linear relationship between the length of the seedlings of large massifs of seeds and their bulk volume in water [4].

The assessment of the reduction in toxicosis of the treated soil was determined by the change in inhibition, as the difference between the inhibition of seed development in the soil with toxicosis (I1) and the inhibition of seed development in the treated milk whey (or manure) soil (I2) according to the formula:

ΔI = I1-I2.

During the experiments, 30 g of soil was placed on the bottom of a cup with a diameter of 95 mm, then 7.5 g of seeds were placed in an even layer, and 30 g of soil was placed on top. After that, water was uniformly added to the cup from a measuring pipette. Six replicates were used, followed by statistical processing of the results.

Seeds sprouted in the soil were washed from the substrate and placed in portions in a 100 ml measuring cylinder with water, placed on a vibrating table oscillating at a frequency of 50 Hz. After each portion of the germinated seeds was placed in the cylinder, which created an openwork porous structure, a small weight of 8 g in the form of a rubber stopper was placed on them for 15-20 seconds, which led to compaction of the structure. After placing all the sprouted seeds in a cylinder, a weight was placed on them and an additional densification of the structure was carried out by lightly tapping (30-40) the cylinder with seeds on the table. These operations made it possible to create a fairly homogeneous structure, and the lower boundary of the load allowed us to determine the bulk volume with an accuracy of 0.5 ml.

Before conducting experiments to determine the toxicosis of gray forest soil and its reduction using whey and manure, the optimal initial soil and sand moisture were determined, at which tests were carried out. To do this, according to the method described above, the amount of water added to gray forest soil (sand) was determined, which would provide the maximum total length of barley seedlings. The optimal size of the water sample was 13.5 g for soil and 15 g for sand.

The soil was treated with serum by adding 80 g of serum to 800 g of soil and after thorough mixing it was left for 7 days. The soil treatment with manure was carried out by adding to a suspension of 800 g of soil a suspension containing 24 g of manure ¹ in 80 g of water, and after thorough mixing was left for 7 days. After that, the treated soil samples were dried to a moisture content of 21.6% and tests were performed.

It was found that toxicosis of gray forest soil for barley cultivar Nur is 52% (I1), that is, barley seeds in the soil develop more than 2 times slower than their development in sand.

It was also found that toxicosis of gray forest soil for barley cultivar “Nur” after appropriate soil treatment with milk whey is 34% (I2 _sow ), and in the case of soil treatment with manure, the inhibition of seed development was 40% (I2 _nav ).

The assessment of the reduction in toxicosis of the treated soil was determined by the change in inhibition, as the difference between the inhibition of seed development in the soil with toxicosis (I1) and the inhibition of seed development in the treated milk whey (or manure) soil (I2) according to the formula:

ΔI = I1-I2.

The results are presented in table 1.

Inhibition of seed germination and its reduction options (reduction of soil toxicosis) during the development of barley seeds of the Nur variety on gray forest soil

¹ This corresponded to the introduction of manure 80-100 t / ha.

The bulk volume of seeds sprouted in the sand at the same time amounted to 86.5 ml.

It was found that treating the soil with manure reduces the inhibition of seed development by 12%, and treating the soil with milk whey reduces the inhibition by 18%. The results obtained indicate that the use of manure or whey can reduce the toxicosis of gray forest soil caused by soil alleotoxins, while treatment with whey reduces inhibition to values below 40%. Moreover, the use of whey is 1.5 times more effective than using manure.

Literature

1. Krasilnikov N.A. Soil microorganisms and higher plants. M .: Publishing. USSR Academy of Sciences. 1958. p. 352.

2. Grodzinsky A.M. Plant allelopathy and soil fatigue. - Kiev, Naukova Dumka, 1991, p. 432.

3. Statsenko A.P., Grishin G.E., Chernyshov V.E. A method for evaluating soil exhaustion. RF patent No. 2181238, 2002.

4. Fedotov G.N., Shalaev B.C., Batyrev Yu.P. Problems of developing seed development stimulants / Materials of the international scientific-practical conference dedicated to the 100th anniversary of higher forest education in Voronezh and the Central Development Commission of Russia “Ecological and biological foundations of increasing the productivity and sustainability of natural and artificially renewable forest ecosystems”. October 4-6, 2018. Voronezh. 2018.Vol. 1. with. 615-623.

Claims (23)

1. A method for assessing the reduction of soil toxicosis, which includes the following steps: the formation of at least two samples of soil with toxicosis, and one sample of sand; processing one soil sample with a reclamation additive, which is used as whey or manure; the formation of at least three weights of seeds of a grain crop; obtaining a seed sample in the sand by providing contact of the seed sample with sand with the addition of water to achieve the lowest moisture capacity of the sand; obtaining a seed sample in a soil with toxicosis by ensuring that one weighed seed comes into contact with this soil with the addition of water until the soil has the lowest moisture capacity; obtaining a seed sample in the soil treated with the reclamation additive, by ensuring that another portion of the seeds comes into contact with the soil treated with the reclamation additive, with the addition of water until the soil has the lowest moisture capacity; extracting the obtained samples before germination; removal of soil and sand from samples and placement of purified germinated seeds of different samples in identical transparent containers with water; compaction of sprouted seeds in containers by means of vibration in the vertical plane and subsequent impact on the bottom of the container, and after vibration exposure to seed samples, loads of identical mass are placed in the container; determination by height of cargo placement from the bottom of the tank of bulk volumes of germinated seeds from a sample with sand (V1) from a sample with soil with toxicosis (V2), and from a sample with soil treated with whey or manure (V3); determination of the amount of inhibition of seed development for a sample with soil with toxicosis (I1), which is used to judge the degree of soil toxicosis by the formula And1 = ((V1-V2) / (V1-20)) ⋅100%, determination of the amount of inhibition of seed development for a sample with treated soil (I2), which is used to judge the degree of toxicosis of the treated soil by the formula And2 = ((V1-V3) / (V1-20)) ⋅100%, determination of the change in inhibition as the difference between the inhibition of seed development in the soil with toxicosis (I1) and the inhibition of seed development in the soil treated with ameliorative additive (I2), which is used to judge the degree of toxicosis of the soil treated with ameliorative additive by the formula: ΔI = I1-I2, at the same time, the degree of inhibition (ΔI) is used to judge the degree of reduction of soil toxicosis when whey or manure is added. 2. The method according to p. 1, characterized in that the aging of the seeds before germination is carried out at a temperature of 22-25 ° C at 100% humidity for 2 days. 3. The method according to p. 1, characterized in that the placement and compaction of seeds in the container is metered for volumes not exceeding 1 / 5-1 / 4 of the volume of germinated seeds from the sample. 4. The method according to p. 1, characterized in that it is performed in six replicates for all seed samples in the sand, soil with toxicosis, and soil treated with whey or manure. 5. The method according to p. 1, characterized in that the vibrational effect in the vertical plane is carried out with a frequency of not more than 50 Hz and an amplitude of 3-4 mm with an acceptable value of deviations from these values of not more than 10%. 6. The method according to p. 1, characterized in that when impacted, its strength is equal to the force of free fall of a cylindrical container filled with water and seeds on the surface of the table from a height of 1 cm with a permissible deviation from the indicated values of not more than 10%. 7. The method according to p. 1, characterized in that as identical transparent containers use transparent cylinders with a ratio of height and diameter of 5: 8.