RU2638431C1 - Method of increasing medical efficiency for cattle pyroplasmosis with pyro-stop medication in association with laserpuncture - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: PYRO-STOP medication is injected in association with irradiation with a semiconductor infrared pulsed laser of biological active spots with numbers 8, 9, 13, 17 and 22 according to the atlas written by Kazeev G.V. The laser is operated by the "Uzor" apparatus with a length of 890 nm with a pulse frequency of 1000, 1500 and 2000 Hz, in exposure of 0.5; 1.0; 1.5; 2.0 and 2.5 minutes after diagnosis and every 24 hours during the course of treatment.

EFFECT: increase of medical efficiency for cattle pyroplasmosis, high therapeutic activity due to additional biostimulating effect.

3 tbl, 1 dwg

Description

The invention relates to animal husbandry and can be used to increase the therapeutic effectiveness of cattle pyroplasmosis.

A known method of treating pyroplasmosis of cattle with drugs LP-40; PP-40; Neosidine-KM, Dimenazen-70 (Drobina AI “Pyroplasmidosis of cattle. Epizootic situation, treatment, prevention.” Abstract of dissertation for the degree of candidate of veterinary sciences. Stavropol, 2007 - 21 pp.).

There is a method of treating cattle pyroplasmosis with Piro-Stop with symptomatic treatment (VK Lolaev, “Epizootology of cattle pyroplasmosis in the Republic of South Ossetia and improvement of control measures.” Abstract of dissertation for the degree of candidate of veterinary sciences. Makhachkala 2012 - 26 p.).

A known method of processing biologically active points by laser radiation "Pattern" to stimulate the growth, development, resistance of calves and milk productivity of cows (Okazov TA "Growth, development, resistance of calves and milk productivity of cows with laser puncture". Abstract of dissertation for the degree Candidate of Agricultural Sciences, Vladikavkaz, 2009 - 21 p., prototype).

A disadvantage of the known methods is that they do not reveal the effect of the laser puncture method on therapeutic efficacy in cattle pyroplasmosis in combination with symptomatic treatment.

The tasks to which the present claimed invention is directed are to irradiate biologically active points with laser radiation in order to identify the effect of radiant energy on the therapeutic efficacy of drug treatment.

The technical result consists in increasing the therapeutic efficacy of the used antiparasitic drugs by increasing the biostimulating activity by exposing the acupuncture points to the radiation of the Uzor laser.

To achieve a technical result in studying the effect of laser puncture on the therapeutic effectiveness of the Piro-Stop preparation, four groups were formed, cattle of 5 animals each: animals older than 1 year old, patients with pyroplasmosis.

To identify sick animals, a clinical examination, thermometry of sick animals, and studies of peripheral blood smears were performed, in which the causative agent of pyroplasmosis Piroplasma bigeminum was isolated.

After the diagnosis was made, search experiments were carried out using the specific Piro-Stop preparation with symptomatic treatment, which revealed a higher therapeutic efficacy of the Piro-Stop preparation in combination with irradiating biologically active points with the Uzor semiconductor infrared pulsed laser with a wavelength of 890 nm, in the exposure: 0.5; 1.0; 2.0 and 2.5 minutes, with a pulse frequency of 1000, 1500 and 2000 Hz immediately after diagnosis and every 24 hours.

To study the effect of laser puncture on the therapeutic efficacy of drug treatment for pyroplasmosis and physiological parameters in the formed groups of cattle, the “Pattern” laser irradiated acupuncture points in the head and trunk under numbers 8, 9, 13, 17 and 22 at atlas Kazeeva G.V. (Fig. 1), selected as the most active for biostimulation of the general condition of the body according to published data.

Animals of all groups were in the same conditions of feeding and keeping.

The therapeutic efficacy of the traditional method of treating cattle pyroplasmosis with the Piro-Stop preparation and symptomatic therapeutic agents is widely used in veterinary practice ..

Summarizing the literature data, it can be noted that since the 50s of the 20th century, serious attention has been paid to the symptomatic treatment of pyroplasmosis. For symptomatic treatment, it is recommended to use, depending on the clinical condition of patients and the period of the disease, a 10% solution of calcium chloride intravenously, as well as glucose and caffeine (S.Ya. Lyubashenko, 1956; B.A. Timofeev, 1977), with caffeine, they suggested use a 10-20% camphor oil emulsion subcutaneously in a dose of 1-2 ml, and deep cleansing enemas (volume 2-3 l).

When organizing symptomatic and pathogenetic treatment, it is necessary to take into account the fact that the used etiotropic drugs increase the toxic load on the systems and individual organs, mainly on the liver, kidneys and nervous system (Nikitina E.A. et al., 2002), and G.G. . Shershel, N.F. Karasev (2004), along with specific therapy, is recommended to introduce a 0.9% solution of sodium chloride.

Given the tasks of studying therapeutic efficacy, the material and methodology of some of our studies, of therapeutic efficacy in cattle pyroplasmosis, dictated the need to study the use of Piro-Stop using traditional methods (control animals).

Piro-Stop is an antiprose drug, it contains imidocarb dipropinate, which has an active effect on Piroplasma bigemina (Babesia bigemina) and is widely used both in the treatment and prevention of cattle pyroplasmosis.

Slaughter for meat of sick animals that were injected with oxytetracycline hydrochloride is allowed no earlier than 6 days after replacing the injection, and animal meat after forced slaughter before the specified time is used as food for carnivores or for the production of meat and bone meal.

It is recommended to use milk from the milk stock of animals during the use of oxytetracycline hydrochloride and before the expiration of 2 days after cessation of use, and if the time is not met, it can also be used for feeding animals.

When used for therapeutic purposes, Piro-Stop, when used with oxytetracycline hydrochloride, it is recommended that the existing safety and personal hygiene rules provided for when working with veterinary products be observed.

In order to make a diagnosis, a clinical examination, thermometry and a study of peripheral blood smears were performed.

Studies were conducted in areas of the Republic of South Ossetia in 2012-2014.

To determine the therapeutic efficacy, 15 goals were treated for a patient with cattle pyroplasmosis (Table 1). Sick animals were characterized by the following clinical signs:

- the incubation period, according to our information, was 6-10 days,

- the patients developed an oppressed state, lacked appetite, increased moisture and ruffled hair, milk yield decreased sharply (from 13 to 2 liters).

In sick animals:

- body temperature 40.0-41.0 ° C;

- increased heart rate to 83-86 beats per minute;

- breathing reached 30-35 movements in one minute;

- mucous membranes anemic, which were replaced by mild yellowness;

- the lymph nodes enlarged, but more often pre-scapular, hypotension and atony of the pancreas develops;

- diarrhea, which is replaced by constipation;

- Pyroplasm was established in peripheral blood smears.

Treatment of sick animals began immediately after diagnosis. The clinical features identified by us are reflected in table 2.

Pyroplasmosis in the study area was recorded in experimental animals from mid-April to mid-October, mainly by three outbreaks: 1 in April-May, 2 in July-August and 3 in September-October.

Our studies have shown that young animals under the age of 1 year are more often sick than animals older than 2 years.

Pyroplasmosis manifests itself after 10-15 days after the animals have been driven to pasture. Patients have characteristic clinical signs, body temperature rises during the entire period of the disease to 41-42 ° C with rare fluctuations. Patients have reduced appetite, they refuse to feed and respond poorly to external stimuli, often lie down, lag behind the herd, get up with difficulty, lose weight, hungry fossa sunken. In cows, milking drastically decreases during milking. Visible mucous membranes of patients are hyperemic, by the end of the first day they become icteric, sometimes with spotty hemorrhages. On the second day in patients, the urine turns light red, on the third - dark cherry, and protein appears in it, urination is abated, which is painful. Patients do not have chewing gum, signs of persistent atony and very rarely hypotension develop, diarrhea appears.

The critical period in patients with pyroplasmosis comes on 2-3 days, after charging the animals with invested ticks lasts up to - 6-12 days. Mortality of pyroplasmosis in animals can be up to 15-20%.

As a rule, after 5 days, as the patients recovered, clinical signs disappeared and a decrease in body temperature was observed, recovery of chewing gum, intestinal motility, rumination, appetite improves, and urine acquires a straw-yellow color.

Pyroplasmosis is easier for indigenous animals, young animals and well-fed cattle, and patients during the spring outbreak, animals are seriously ill during summer epizootics.

We treated pyroplasmosis with a five-day regimen, in which, along with etiotropic symptoms, special attention was paid to treatment, taking into account the symptomatic signs and the clinical picture.

After confirming the diagnosis of pyroplasmosis, all animals were injected with an antiparasitic drug and an intravenous glucose solution with calcium chloride and ascorbic acid, subcutaneous sodium benzoic caffeine, inside, up to 0.5 liters of vegetable oil, white hellebore extract, whole body massage was given, water was given freely. Feeding used meadow grass. Sick animals were kept under a canopy or in a well-ventilated area. In experimental animals, the clinical condition was serious, and typical signs were established in them: general body temperature, pulse rate, and respiratory rate were increased.

On the second day of the disease, the state of the animals remains depressed, they refuse to take food, urine becomes red-cherry, diarrhea, pulse and breathing become more frequent, body temperature remains elevated (41.0-41.4).

On the third day of the disease, atony of the pancreas develops, urine contains an admixture of blood, and the frequency of urination increases, feces becomes mixed with mucus, visible mucous membranes become icteric, and body temperature in all animals ranges from 39.5 to 40.4 ° C.

On the fourth day, the clinical condition improves, the animals begin to respond to external factors, the appetite is restored, frequent urination, the atria of the stomach are weak, the mucous membranes become icteric with punctate hemorrhages, romania is restored, the body temperature in most sick animals approaches the physiological norm and keeps.

The fifth day of the disease is characterized by normalization of the clinical signs of animals, indicators of body temperature, pulse rate and respiration become within the physiological norm. The appetite is restored and by the sixth day of sick animals are released into the pasture. Based on the research results, we developed a block diagram of the algorithm of the scientific and practical concept for the localization of natural foci of animal protozoa in the conditions of RSO.

The dynamics of the clinical manifestation of pyroplasmosis with the use of Piro-Stop and in combination with laser puncture.

To indicate the results of the clinical condition of experimental animals, the following notation was used:

HC - characteristic clinical signs,

KS - clinical signs are poorly expressed.

KO - no clinical signs,

RV - animals died.

The results of the study showed that in the therapeutic efficacy of pyroplasmosis in experimental cattle when using Piro-Stop separately and in combination with laser puncture, significant differences were found, which are reflected in table 3.

It was established that in the clinical picture of the sick experimental livestock of the control group during treatment with Piro-Stop with Piro-Stop during the first three days of the disease, no significant differences were found and characteristic clinical signs were recorded in all experimental animals.

On the fourth day of treatment, out of 55 control cattle, forty-eight showed characteristic clinical signs (87.3%), six registered mild clinical signs (21.8%) and one head fell (1.8%).

In a five-day control experimental livestock of the studied animals (54 heads), and a characteristic clinic was established in forty-one heads (75.9%), with thinned signs of the studied disease in thirteen s (24.1%). In six-day control animals, pronounced signs of pyroplasmosis were not found in nine animals (16.7%), eight showed characteristic signs of the studied invasion (14.8%), thirty-seven registered weak clinical signs (68.5%), on the seventh day in the experimental control livestock there were no deviations in the clinical condition and actively responded to external factors.

Comparison of the results of the use of Piro-Stop and its combination with laser puncture showed that there were no differences in clinical signs up to two days of treatment, both control and experimental experimental animals were characterized by the presence of characteristic signs of pyroplasmosis.

On the third day in the clinical condition of the control and experimental groups of animals significant differences were established. All control animals (55 animals) showed characteristic clinical signs, while in all laser puncture modes (1000; 1500 and 2000 Hz) a characteristic clinic was established in fifty-four animals (98.2%) with a single case (1.8 %).

On the fourth day of treatment measures out of 54 animals, if the control animals recorded forty-eight animals (88.9%) with characteristic signs of the studied invasion, in the experimental groups this indicator was thirty-one animals (57.4%), which is lower than the indicator control animals for seventeen goals or 31.5%, in experimental experimental animals at this period of research clinical signs were found that were weakly expressed in twenty-three goals (42.6%).

The fifth day of studying the clinical status of sick animals showed that if control animals showed characteristic clinical signs in forty one heads (75.9%), insufficient clinical signs in thirteen heads (24.1%), then in the experimental groups of experimental animals in clinical deviations from the norm have not been established: twenty-five goals (46.3%), twenty-two showed mild signs of the disease (40.7%), a characteristic clinic was found in seven animals (13.0%).

On the sixth day of the studied invasion in the experimental groups of animals, deviations of the clinical status were not established, while control analogues of 54 did not show deviations of the clinical condition from the norm, nine goals (16.7%), a weakly expressed clinic in thirty-seven (68.5 %) and a characteristic clinic in eight sick animals (14.8%).

On the seventh day, among the control animals, deviations of clinical status were not established.

Studies have shown that higher results of therapeutic efficacy in pyroplasmosis were obtained in the laser puncture mode of 1000 Hz at exposures of 1.0 and 1.5 minutes, 1500 Hz at a dose of 1.0 and 1.5 minutes, 2000 Hz, therapeutic efficacy was higher in exposure 1.5 minutes

Summarizing the results of the analysis of the clinical condition of the experimental groups of animals treated with the Piro-Stop preparation, on the one hand, and its combination with treatment with the Uzor laser light, biologically active points, on the other hand, we can conclude:

- laser effects on the BAT in the application modes of 1000, 1500 and 2000 Hz in exposures from 0.5 to 2.5 minutes have a significant positive effect on the course of bovine pyroplasmosis;

- the use of exposure laser effects from 0.5 to 2.5 minutes in 1000 Hz application modes has established a higher therapeutic activity in exposures of 1.0 and 1.5 minutes with slight superiority of the latter;

- on the clinical status of experimental cattle in the mode of using 1500 Hz, all exposure doses were effective (from 0.5 to 1.5 minutes);

- in the dynamics of the clinical condition of experimental animals using 2000 Hz, higher results showed the use of 1.0 and 1.5 minute exposures without significant differences between them;

- studies to identify a higher regimen of use of the Piro-Stop drug in combination with laser puncture dictate the need for additional research in the conditions of animals in different economic conditions, which allow to determine higher results of therapeutic efficacy of laser puncture in different modes of use.

Claims (1)

A method of increasing the therapeutic efficacy of bovine pyroplasmosis with the Piro-Stop preparation in combination with laser puncture, including the use of the Piro-Stop preparation for treating bovine pyroplasmosis with the symptomatic treatment, characterized in that the Piro-Stop treatment is combined with irradiation of biologically active points under the numbers 8, 9, 13, 17 and 22 in the atlas Kazeeva G.V. semiconductor infrared pulsed laser "Pattern" with a length of 890 nm, a pulse frequency of 1000, 1500 and 2000 Hz, in exposures of 0.5; 1.0; 1.5; 2.0 and 2.5 minutes after diagnosis and every 24 hours during treatment.

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