RU2596476C1 - Method of producing nanocapsules of antispasmodic medicinal plants - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to a method of producing nanocapsules of antispasmodic medicinal plants, characterised by the fact that sodium alginate is used for the shell, and antispasmodic medicinal plants are used in the core. In the exercise of this method, antispasmodic medicinal plant tincture is stirred into a sodium alginate suspension in benzene in the presence of glycerol ester with one or two molecules of edible fatty acids and one or two molecules of citric acid, then a hexane is added. Resulting suspension is filtered and dried at room temperature, wherein the nucleus to shell ratio is 1:3 or 1:1.

EFFECT: technical result consists in simplifying and accelerating the process of producing nanocapsules and high mass output.

1 cl, 3 ex, 1 dwg

Description

The invention relates to the field of nanotechnology, medicine and the food industry.

Previously known methods for producing microcapsules.

In US Pat. RF 2173140, IPC A61K 009/50, A61K 009/127, publ. 09/10/2001, a method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar phenomena of sound and ultrasonic range for dispersion is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin

In US Pat. RF 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, publ. 06/27/2009, a method is proposed for producing sodium chloride microcapsules using spray cooling in a Niro spray tower under the following conditions: inlet air temperature 10 ° C, outlet air temperature 28 ° C, spray drum rotation speed of 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. RF 2134967, IPC A01N 53/00, A01N 25/28, publ. 08/27/1999. A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution to the technical problem is achieved by the method of producing nanocapsules of medicinal plants with an antispasmodic effect, characterized in that sodium alginate is used as the shell of the nanocapsules, and peppermint is used as the core when nanocapsules are prepared by the non-solvent precipitation method using hexane as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using hexane as a precipitant, as well as the use of sodium alginate as a shell of particles and tinctures of medicinal plants with antispasmodic action - as a core.

The result of the proposed method is to obtain nanocapsules of medicinal plants with antispasmodic action.

EXAMPLE 1. Obtaining nanocapsules of mint tincture, the ratio of core: shell is 1: 3.

10 ml of mint tincture is added to a suspension of sodium alginate in benzene containing 3 g of the specified polymer in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid), with citric acid, as tribasic, it can be esterified with other glycerides and as an oxo acid with other fatty acids. Free acid groups can be neutralized with sodium as a surfactant with stirring at 1300 rpm. Then 10 ml of hexane are added. The resulting suspension is filtered and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2. Obtaining nanocapsules of mint tincture, the ratio of core: shell is 1: 1.

10 ml of valerian tincture is added to a suspension of sodium alginate in benzene containing 1 g of the indicated polymer in the presence of 0.01 g of the preparation E472c as a surfactant, with stirring 1300 rpm. Then add 6 ml of hexane. The resulting suspension is filtered and dried at room temperature.

Received 2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3. Sizing of nanocapsules by the NTA method (Fig. 1).

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the Nanoparticle Tracking Analysis (NTA) method described in ASTM E2834.

The optimal dilution for dilution was 1: 100. For the measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto. Duration of a single measurement of 215s, the use of a syringe pump.

Claims (1)

A method of producing nanocapsules of medicinal plants having an antispasmodic effect, characterized in that sodium alginate is used as a shell, and medicinal plants having an antispasmodic effect are used as a core. When implementing the method, tincture of a medicinal plant having an antispasmodic effect is added to the sodium alginate suspension in benzene in the presence of glycerol ester with one to two molecules of food fatty acids and with one or two molecules of citric slots, with stirring, then poured in hexane, the resulting suspension was filtered and dried at room temperature, wherein the ratio of core: sheath ratio is 1: 3 or 1: 1.

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