RU2577143C2 - Детекция aad-1 объекта das-40278-9 - Google Patents
Детекция aad-1 объекта das-40278-9 Download PDFInfo
- Publication number
- RU2577143C2 RU2577143C2 RU2012110253/10A RU2012110253A RU2577143C2 RU 2577143 C2 RU2577143 C2 RU 2577143C2 RU 2012110253/10 A RU2012110253/10 A RU 2012110253/10A RU 2012110253 A RU2012110253 A RU 2012110253A RU 2577143 C2 RU2577143 C2 RU 2577143C2
- Authority
- RU
- Russia
- Prior art keywords
- corn
- seq
- dna
- sequence
- specified
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title description 18
- 101100442689 Caenorhabditis elegans hdl-1 gene Proteins 0.000 title description 4
- 238000000034 method Methods 0.000 claims abstract description 99
- 239000000523 sample Substances 0.000 claims abstract description 86
- 230000009261 transgenic effect Effects 0.000 claims abstract description 37
- 241001057636 Dracaena deremensis Species 0.000 claims abstract description 22
- 239000007850 fluorescent dye Substances 0.000 claims abstract 5
- 240000008042 Zea mays Species 0.000 claims description 94
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 90
- 108020004414 DNA Proteins 0.000 claims description 82
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 45
- 235000005822 corn Nutrition 0.000 claims description 45
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 44
- 235000009973 maize Nutrition 0.000 claims description 44
- 108091093088 Amplicon Proteins 0.000 claims description 40
- 230000000295 complement effect Effects 0.000 claims description 19
- 238000003753 real-time PCR Methods 0.000 claims description 12
- 108010028143 Dioxygenases Proteins 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 238000004445 quantitative analysis Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 41
- 230000000694 effects Effects 0.000 abstract description 2
- 238000010276 construction Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000013615 primer Substances 0.000 description 74
- 241000196324 Embryophyta Species 0.000 description 54
- 150000007523 nucleic acids Chemical class 0.000 description 38
- 108091028043 Nucleic acid sequence Proteins 0.000 description 34
- 238000004458 analytical method Methods 0.000 description 30
- 238000009396 hybridization Methods 0.000 description 28
- 238000003199 nucleic acid amplification method Methods 0.000 description 28
- 102000039446 nucleic acids Human genes 0.000 description 28
- 108020004707 nucleic acids Proteins 0.000 description 28
- 230000003321 amplification Effects 0.000 description 27
- 238000003752 polymerase chain reaction Methods 0.000 description 25
- 239000004009 herbicide Substances 0.000 description 22
- 239000002987 primer (paints) Substances 0.000 description 20
- 125000003729 nucleotide group Chemical group 0.000 description 17
- 239000002773 nucleotide Substances 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 13
- 230000002363 herbicidal effect Effects 0.000 description 11
- 108700019146 Transgenes Proteins 0.000 description 10
- 108091092584 GDNA Proteins 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 8
- 239000012634 fragment Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 235000013305 food Nutrition 0.000 description 7
- 239000003112 inhibitor Substances 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 6
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000003976 plant breeding Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 230000009418 agronomic effect Effects 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 238000009402 cross-breeding Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000010153 self-pollination Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 241000219194 Arabidopsis Species 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 108020001019 DNA Primers Proteins 0.000 description 3
- 230000004544 DNA amplification Effects 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 108020001991 Protoporphyrinogen Oxidase Proteins 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 235000021306 genetically modified maize Nutrition 0.000 description 3
- 239000001573 invertase Substances 0.000 description 3
- 235000011073 invertase Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000000306 recurrent effect Effects 0.000 description 3
- 230000001568 sexual effect Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000011895 specific detection Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- 102000016680 Dioxygenases Human genes 0.000 description 2
- 239000005561 Glufosinate Substances 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 239000005562 Glyphosate Substances 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 102100029028 Protoporphyrinogen oxidase Human genes 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 235000007244 Zea mays Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 235000003869 genetically modified organism Nutrition 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- 229940097068 glyphosate Drugs 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 238000009401 outcrossing Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- MPKRXENUURLWTO-UHFFFAOYSA-N 1-phenyl-7,9-dihydro-3h-purine-2,6,8-trione Chemical compound O=C1C=2NC(=O)NC=2NC(=O)N1C1=CC=CC=C1 MPKRXENUURLWTO-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- GQQIAHNFBAFBCS-UHFFFAOYSA-N 2-[2-chloro-5-(1,3-dioxo-4,5,6,7-tetrahydroisoindol-2-yl)-4-fluorophenoxy]acetic acid Chemical compound C1=C(Cl)C(OCC(=O)O)=CC(N2C(C3=C(CCCC3)C2=O)=O)=C1F GQQIAHNFBAFBCS-UHFFFAOYSA-N 0.000 description 1
- OOLBCHYXZDXLDS-UHFFFAOYSA-N 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoic acid Chemical compound C1=CC(OC(C)C(O)=O)=CC=C1OC1=CC=C(Cl)C=C1Cl OOLBCHYXZDXLDS-UHFFFAOYSA-N 0.000 description 1
- OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
- YHKBGVDUSSWOAB-UHFFFAOYSA-N 2-chloro-3-{2-chloro-5-[4-(difluoromethyl)-3-methyl-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl]-4-fluorophenyl}propanoic acid Chemical compound O=C1N(C(F)F)C(C)=NN1C1=CC(CC(Cl)C(O)=O)=C(Cl)C=C1F YHKBGVDUSSWOAB-UHFFFAOYSA-N 0.000 description 1
- CDUVSERIDNVFDD-UHFFFAOYSA-N 2-pyrimidin-2-ylbenzenecarbothioic s-acid Chemical class OC(=S)C1=CC=CC=C1C1=NC=CC=N1 CDUVSERIDNVFDD-UHFFFAOYSA-N 0.000 description 1
- UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- 101150111419 ATH1 gene Proteins 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 108010007730 Apyrase Proteins 0.000 description 1
- 102000007347 Apyrase Human genes 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100433747 Arabidopsis thaliana ABCA2 gene Proteins 0.000 description 1
- 101100288148 Arabidopsis thaliana KNAT5 gene Proteins 0.000 description 1
- 101100135611 Arabidopsis thaliana PAP12 gene Proteins 0.000 description 1
- 101150002428 Atoh1 gene Proteins 0.000 description 1
- 239000005489 Bromoxynil Substances 0.000 description 1
- 101100057132 Candida albicans (strain SC5314 / ATCC MYA-2876) ATC1 gene Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 239000005504 Dicamba Substances 0.000 description 1
- 239000005506 Diclofop Substances 0.000 description 1
- QNXAVFXEJCPCJO-UHFFFAOYSA-N Diclosulam Chemical compound N=1N2C(OCC)=NC(F)=CC2=NC=1S(=O)(=O)NC1=C(Cl)C=CC=C1Cl QNXAVFXEJCPCJO-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- RXCPQSJAVKGONC-UHFFFAOYSA-N Flumetsulam Chemical compound N1=C2N=C(C)C=CN2N=C1S(=O)(=O)NC1=C(F)C=CC=C1F RXCPQSJAVKGONC-UHFFFAOYSA-N 0.000 description 1
- 239000005532 Flumioxazine Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 101001091419 Ipomoea batatas Polyphenol oxidase I, chloroplastic Proteins 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 239000005578 Mesotrione Substances 0.000 description 1
- 239000005582 Metosulam Substances 0.000 description 1
- VGHPMIFEKOFHHQ-UHFFFAOYSA-N Metosulam Chemical compound N1=C2N=C(OC)C=C(OC)N2N=C1S(=O)(=O)NC1=C(Cl)C=CC(C)=C1Cl VGHPMIFEKOFHHQ-UHFFFAOYSA-N 0.000 description 1
- 101001123254 Nicotiana tabacum Protoporphyrinogen oxidase, chloroplastic Proteins 0.000 description 1
- 102000008297 Nuclear Matrix-Associated Proteins Human genes 0.000 description 1
- 108010035916 Nuclear Matrix-Associated Proteins Proteins 0.000 description 1
- 239000005590 Oxyfluorfen Substances 0.000 description 1
- OQMBBFQZGJFLBU-UHFFFAOYSA-N Oxyfluorfen Chemical compound C1=C([N+]([O-])=O)C(OCC)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 OQMBBFQZGJFLBU-UHFFFAOYSA-N 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 108010081996 Photosystem I Protein Complex Proteins 0.000 description 1
- 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 102000005135 Protoporphyrinogen oxidase Human genes 0.000 description 1
- 108020004518 RNA Probes Proteins 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 241001292337 Sphingobium herbicidovorans Species 0.000 description 1
- 239000005618 Sulcotrione Substances 0.000 description 1
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 description 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
- 239000008051 TBE buffer Substances 0.000 description 1
- 102100029373 Transcription factor ATOH1 Human genes 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- 108010055615 Zein Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- NUFNQYOELLVIPL-UHFFFAOYSA-N acifluorfen Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 NUFNQYOELLVIPL-UHFFFAOYSA-N 0.000 description 1
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 description 1
- IRLPACMLTUPBCL-FCIPNVEPSA-N adenosine-5'-phosphosulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@@H](CO[P@](O)(=O)OS(O)(=O)=O)[C@H](O)[C@H]1O IRLPACMLTUPBCL-FCIPNVEPSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- JEDYYFXHPAIBGR-UHFFFAOYSA-N butafenacil Chemical compound O=C1N(C)C(C(F)(F)F)=CC(=O)N1C1=CC=C(Cl)C(C(=O)OC(C)(C)C(=O)OCC=C)=C1 JEDYYFXHPAIBGR-UHFFFAOYSA-N 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000005081 chemiluminescent agent Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- BIKACRYIQSLICJ-UHFFFAOYSA-N cloransulam-methyl Chemical group N=1N2C(OCC)=NC(F)=CC2=NC=1S(=O)(=O)NC1=C(Cl)C=CC=C1C(=O)OC BIKACRYIQSLICJ-UHFFFAOYSA-N 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 108010027183 cytochrome P-450 CYP76B1 (Helianthus tuberosus) Proteins 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical class C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 description 1
- 238000007848 endpoint PCR Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- WFZSZAXUALBVNX-UHFFFAOYSA-N flufenpyr Chemical compound O=C1C(C)=C(C(F)(F)F)C=NN1C1=CC(OCC(O)=O)=C(Cl)C=C1F WFZSZAXUALBVNX-UHFFFAOYSA-N 0.000 description 1
- FOUWCSDKDDHKQP-UHFFFAOYSA-N flumioxazin Chemical compound FC1=CC=2OCC(=O)N(CC#C)C=2C=C1N(C1=O)C(=O)C2=C1CCCC2 FOUWCSDKDDHKQP-UHFFFAOYSA-N 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 238000002875 fluorescence polarization Methods 0.000 description 1
- BGZZWXTVIYUUEY-UHFFFAOYSA-N fomesafen Chemical compound C1=C([N+]([O-])=O)C(C(=O)NS(=O)(=O)C)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 BGZZWXTVIYUUEY-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- -1 isoxaflutol Chemical compound 0.000 description 1
- CONWAEURSVPLRM-UHFFFAOYSA-N lactofen Chemical compound C1=C([N+]([O-])=O)C(C(=O)OC(C)C(=O)OCC)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 CONWAEURSVPLRM-UHFFFAOYSA-N 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- KPUREKXXPHOJQT-UHFFFAOYSA-N mesotrione Chemical compound [O-][N+](=O)C1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O KPUREKXXPHOJQT-UHFFFAOYSA-N 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- OKCDZCGCKMTKMK-UHFFFAOYSA-N n-phenyl-2h-triazolo[4,5-d]pyrimidine-5-sulfonamide Chemical compound N=1C=C2NN=NC2=NC=1S(=O)(=O)NC1=CC=CC=C1 OKCDZCGCKMTKMK-UHFFFAOYSA-N 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 210000000299 nuclear matrix Anatomy 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- ONDBSQWZWRGVIL-UHFFFAOYSA-N o-pyrimidin-2-yl benzenecarbothioate Chemical class C=1C=CC=CC=1C(=S)OC1=NC=CC=N1 ONDBSQWZWRGVIL-UHFFFAOYSA-N 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 108010001545 phytoene dehydrogenase Proteins 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 101150075980 psbA gene Proteins 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 238000011158 quantitative evaluation Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- PQTBTIFWAXVEPB-UHFFFAOYSA-N sulcotrione Chemical compound ClC1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O PQTBTIFWAXVEPB-UHFFFAOYSA-N 0.000 description 1
- OORLZFUTLGXMEF-UHFFFAOYSA-N sulfentrazone Chemical compound O=C1N(C(F)F)C(C)=NN1C1=CC(NS(C)(=O)=O)=C(Cl)C=C1Cl OORLZFUTLGXMEF-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- YWBFPKPWMSWWEA-UHFFFAOYSA-O triazolopyrimidine Chemical compound BrC1=CC=CC(C=2N=C3N=CN[N+]3=C(NCC=3C=CN=CC=3)C=2)=C1 YWBFPKPWMSWWEA-UHFFFAOYSA-O 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23524809P | 2009-08-19 | 2009-08-19 | |
| US61/235,248 | 2009-08-19 | ||
| US23736610P | 2010-04-23 | 2010-04-23 | |
| US61/237,366 | 2010-04-23 | ||
| PCT/US2010/045871 WO2011022471A1 (en) | 2009-08-19 | 2010-08-18 | Detection of aad-1 event das-40278-9 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2012110253A RU2012110253A (ru) | 2013-09-27 |
| RU2577143C2 true RU2577143C2 (ru) | 2016-03-10 |
Family
ID=43607318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012110253/10A RU2577143C2 (ru) | 2009-08-19 | 2010-08-18 | Детекция aad-1 объекта das-40278-9 |
Country Status (14)
| Country | Link |
|---|---|
| JP (1) | JP5771208B2 (enExample) |
| KR (1) | KR101772638B1 (enExample) |
| CN (1) | CN102575299B (enExample) |
| AU (1) | AU2010284286B2 (enExample) |
| CA (1) | CA2771581C (enExample) |
| CL (1) | CL2012000410A1 (enExample) |
| CO (1) | CO6511214A2 (enExample) |
| IL (1) | IL218175A (enExample) |
| IN (1) | IN2012DN01800A (enExample) |
| MX (1) | MX336072B (enExample) |
| NZ (1) | NZ598598A (enExample) |
| RU (1) | RU2577143C2 (enExample) |
| WO (1) | WO2011022471A1 (enExample) |
| ZA (1) | ZA201201210B (enExample) |
Families Citing this family (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8598413B2 (en) * | 2009-08-19 | 2013-12-03 | Dow AgroSciecnes, LLC. | AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1 |
| IN2012DN02294A (enExample) | 2009-08-19 | 2015-08-21 | Dow Agrosciences Llc | |
| TWI667347B (zh) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法 |
| EA201691886A1 (ru) * | 2014-03-20 | 2017-01-30 | МОНСАНТО ТЕКНОЛОДЖИ ЭлЭлСи | Трансгенный трансформант кукурузы mon 87419 и способы его применения |
| GB201622007D0 (en) | 2016-12-22 | 2017-02-08 | And See Cambridge Display Tech Ltd Syngenta Participations Ag | Polymorphs |
| HRP20210497T1 (hr) | 2016-12-22 | 2021-05-14 | Syngenta Participations Ag | Polimorfi |
| UY37623A (es) | 2017-03-03 | 2018-09-28 | Syngenta Participations Ag | Derivados de oxadiazol tiofeno fungicidas |
| BR112019020134B1 (pt) | 2017-03-31 | 2023-05-09 | Syngenta Participations Ag | Composições fungicidas |
| CN113979962A (zh) | 2017-03-31 | 2022-01-28 | 先正达参股股份有限公司 | 杀真菌组合物 |
| BR112019020693B1 (pt) | 2017-04-03 | 2023-11-28 | Syngenta Participations Ag | Compostos derivados de oxadiazol microbiocidas, suas composições, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos utilizando tais compostos e seus usos |
| BR112019020756B1 (pt) | 2017-04-05 | 2023-11-28 | Syngenta Participations Ag | Compostos derivados de oxadiazol microbicidas, composição agroquímica compreendendo os mesmos, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos e uso desses compostos |
| WO2018184988A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018184985A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018184987A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018184986A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| BR112019020735B1 (pt) | 2017-04-05 | 2023-12-05 | Syngenta Participations Ag | Compostos derivados de oxadiazol microbiocidas e seu uso, composição agroquímica e método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos |
| WO2018185211A1 (en) | 2017-04-06 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018219773A1 (en) | 2017-06-02 | 2018-12-06 | Syngenta Participations Ag | Fungicidal compositions |
| WO2018219825A1 (en) | 2017-06-02 | 2018-12-06 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| US11154058B2 (en) | 2017-06-14 | 2021-10-26 | Syngenta Participations Ag | Fungicidal compositions |
| CA3067309A1 (en) | 2017-06-28 | 2019-01-03 | Syngenta Participations Ag | Fungicidal compositions |
| WO2019011926A1 (en) | 2017-07-11 | 2019-01-17 | Syngenta Participations Ag | MICROBIOCIDE OXADIAZOLE DERIVATIVES |
| WO2019011928A1 (en) | 2017-07-11 | 2019-01-17 | Syngenta Participations Ag | MICROBIOCIDE OXADIAZOLE DERIVATIVES |
| WO2019011929A1 (en) | 2017-07-11 | 2019-01-17 | Syngenta Participations Ag | MICROBIOCIDE OXADIAZOLE DERIVATIVES |
| BR112020000465B1 (pt) | 2017-07-11 | 2024-02-20 | Syngenta Participations Ag | Derivados oxadiazol microbiocidas |
| WO2019012011A1 (en) | 2017-07-12 | 2019-01-17 | Syngenta Participations Ag | MICROBIOCIDE OXADIAZOLE DERIVATIVES |
| WO2019012001A1 (en) | 2017-07-12 | 2019-01-17 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| BR112020000463A2 (pt) | 2017-07-13 | 2020-07-21 | Syngenta Participations Ag | derivados oxadiazol microbiocidas |
| UY37913A (es) | 2017-10-05 | 2019-05-31 | Syngenta Participations Ag | Derivados de picolinamida fungicidas que portan un grupo terminal cuaternario |
| UY37912A (es) | 2017-10-05 | 2019-05-31 | Syngenta Participations Ag | Derivados de picolinamida fungicidas que portan grupos terminales heteroarilo o heteroariloxi |
| WO2019096709A1 (en) | 2017-11-15 | 2019-05-23 | Syngenta Participations Ag | Microbiocidal picolinamide derivatives |
| WO2019207062A1 (en) | 2018-04-26 | 2019-10-31 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| US20210269426A1 (en) | 2018-06-29 | 2021-09-02 | Syngenta Crop Protection Ag | Microbiocidal oxadiazole derivatives |
| EP3818058A1 (en) | 2018-07-02 | 2021-05-12 | Syngenta Crop Protection AG | 3-(2-thienyl)-5-(trifluoromethyl)-1,2,4-oxadiazole derivatives as agrochemical fungicides |
| CN112689631A (zh) | 2018-07-16 | 2021-04-20 | 先正达农作物保护股份公司 | 杀微生物的噁二唑衍生物 |
| CN113195462A (zh) | 2018-10-17 | 2021-07-30 | 先正达农作物保护股份公司 | 杀微生物的噁二唑衍生物 |
| AR116628A1 (es) | 2018-10-18 | 2021-05-26 | Syngenta Crop Protection Ag | Compuestos microbiocidas |
| WO2020165403A1 (en) | 2019-02-15 | 2020-08-20 | Syngenta Crop Protection Ag | Phenyl substituted thiazole derivatives as microbiocidal compounds |
| KR20210132089A (ko) | 2019-02-20 | 2021-11-03 | 신젠타 크롭 프로텍션 아게 | 스피로피디온의 용도 |
| GB201903942D0 (en) | 2019-03-22 | 2019-05-08 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| US20220167615A1 (en) | 2019-04-10 | 2022-06-02 | Syngenta Crop Protection Ag | Fungicidal compositions |
| WO2020208095A1 (en) | 2019-04-10 | 2020-10-15 | Syngenta Crop Protection Ag | Microbiocidal picolinamide derivatives |
| EP3994124A1 (en) | 2019-07-05 | 2022-05-11 | Syngenta Crop Protection AG | Microbiocidal picolinamide derivatives |
| GB201910037D0 (en) | 2019-07-12 | 2019-08-28 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| UY39115A (es) | 2020-03-05 | 2021-10-29 | Syngenta Crop Protection Ag | Mezclas fungicidas de derivados de arilo metoxiacrilato |
| BR112022017254A2 (pt) | 2020-03-05 | 2022-10-18 | Syngenta Crop Protection Ag | Composições fungicidas |
| GB202006386D0 (en) | 2020-04-30 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal Compounds |
| GB202006399D0 (en) | 2020-04-30 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| GB202006480D0 (en) | 2020-05-01 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| GB202006606D0 (en) | 2020-05-05 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| GB202014840D0 (en) | 2020-09-21 | 2020-11-04 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| KR102383881B1 (ko) * | 2020-10-16 | 2022-04-06 | 국립생태원 | 유전자변형 옥수수의 동시 검출 방법 |
| TW202231187A (zh) | 2020-11-27 | 2022-08-16 | 瑞士商先正達農作物保護公司 | 殺有害生物組成物 |
| UY39544A (es) | 2020-12-02 | 2022-06-30 | Syngenta Crop Protection Ag | Composiciones fungicidas que comprenden una mezcla de componentes (a) y (b) como principios activos |
| WO2022117650A1 (en) | 2020-12-02 | 2022-06-09 | Syngenta Crop Protection Ag | Fungicidal compositions |
| AR125089A1 (es) | 2021-03-19 | 2023-06-07 | Syngenta Crop Protection Ag | Composiciones plaguicidas |
| AU2022269255B2 (en) | 2021-05-04 | 2025-09-11 | Syngenta Crop Protection Ag | Use of clethodim for insect control |
| CN117597025A (zh) | 2021-07-02 | 2024-02-23 | 先正达农作物保护股份公司 | 精吡氟禾草灵用于昆虫控制的用途 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005059103A2 (en) * | 2003-12-15 | 2005-06-30 | Monsanto Technology Llc | Corn plant mon88017 and compositions and methods for detection thereof |
| WO2005107437A2 (en) * | 2004-04-30 | 2005-11-17 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| RU2270254C2 (ru) * | 2004-04-30 | 2006-02-20 | Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук | Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа |
| WO2008143993A2 (en) * | 2007-05-17 | 2008-11-27 | Monsanto Technology Llc | Corn polymorphisms and methods of genotyping |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6245974B1 (en) * | 1997-08-06 | 2001-06-12 | North Carolina State University | Matrix attachment regions |
| CL2003002461A1 (es) * | 2002-11-27 | 2005-01-07 | Dow Chemical Company Agroscien | Inmunoglobulina que comprende al menos un glicano afucosilado, composicion que la contiene, secuencia nucleotidica y vector que la comprende, procedimiento para producir dicha inmunoglobulina en plantas. |
| US8598413B2 (en) * | 2009-08-19 | 2013-12-03 | Dow AgroSciecnes, LLC. | AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1 |
| IN2012DN02294A (enExample) * | 2009-08-19 | 2015-08-21 | Dow Agrosciences Llc |
-
2010
- 2010-08-18 AU AU2010284286A patent/AU2010284286B2/en not_active Ceased
- 2010-08-18 NZ NZ598598A patent/NZ598598A/en not_active IP Right Cessation
- 2010-08-18 CA CA2771581A patent/CA2771581C/en active Active
- 2010-08-18 MX MX2012002076A patent/MX336072B/es unknown
- 2010-08-18 CN CN201080047115.6A patent/CN102575299B/zh active Active
- 2010-08-18 WO PCT/US2010/045871 patent/WO2011022471A1/en not_active Ceased
- 2010-08-18 RU RU2012110253/10A patent/RU2577143C2/ru not_active IP Right Cessation
- 2010-08-18 KR KR1020127006871A patent/KR101772638B1/ko not_active Expired - Fee Related
- 2010-08-18 IN IN1800DEN2012 patent/IN2012DN01800A/en unknown
- 2010-08-18 JP JP2012525662A patent/JP5771208B2/ja not_active Expired - Fee Related
-
2012
- 2012-02-16 IL IL218175A patent/IL218175A/en not_active IP Right Cessation
- 2012-02-16 CL CL2012000410A patent/CL2012000410A1/es unknown
- 2012-02-17 ZA ZA2012/01210A patent/ZA201201210B/en unknown
- 2012-03-06 CO CO12039002A patent/CO6511214A2/es not_active Application Discontinuation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005059103A2 (en) * | 2003-12-15 | 2005-06-30 | Monsanto Technology Llc | Corn plant mon88017 and compositions and methods for detection thereof |
| WO2005107437A2 (en) * | 2004-04-30 | 2005-11-17 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| RU2270254C2 (ru) * | 2004-04-30 | 2006-02-20 | Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук | Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа |
| WO2008143993A2 (en) * | 2007-05-17 | 2008-11-27 | Monsanto Technology Llc | Corn polymorphisms and methods of genotyping |
Non-Patent Citations (1)
| Title |
|---|
| GERMAN M.A.et al., A rapid method for the analysis of zygosity in transgenic plants, Plant Science, 2003, Vol. 164, pp. 183-187. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102575299B (zh) | 2015-09-09 |
| IL218175A (en) | 2017-04-30 |
| ZA201201210B (en) | 2012-10-31 |
| JP2013505706A (ja) | 2013-02-21 |
| IN2012DN01800A (enExample) | 2015-06-05 |
| MX336072B (es) | 2016-01-06 |
| KR101772638B1 (ko) | 2017-08-31 |
| CL2012000410A1 (es) | 2012-10-19 |
| AU2010284286A1 (en) | 2012-03-29 |
| NZ598598A (en) | 2014-05-30 |
| MX2012002076A (es) | 2012-03-21 |
| AU2010284286B2 (en) | 2015-08-20 |
| IL218175A0 (en) | 2012-07-31 |
| CO6511214A2 (es) | 2012-08-31 |
| CA2771581C (en) | 2018-05-01 |
| CA2771581A1 (en) | 2011-02-24 |
| BR112012003873A2 (pt) | 2016-11-22 |
| RU2012110253A (ru) | 2013-09-27 |
| HK1173194A1 (zh) | 2013-05-10 |
| WO2011022471A1 (en) | 2011-02-24 |
| KR20120053038A (ko) | 2012-05-24 |
| CN102575299A (zh) | 2012-07-11 |
| JP5771208B2 (ja) | 2015-08-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2577143C2 (ru) | Детекция aad-1 объекта das-40278-9 | |
| US9353381B2 (en) | Cotton event pDAB4468.19.10.3 detection method | |
| WO2011066360A1 (en) | Detection of aad-12 soybean event 416 | |
| RU2573898C2 (ru) | Детектирование aad-12-события 416 у сои | |
| US20110151441A1 (en) | Endpoint taqman methods for determining zygosity of corn comprising tc1507 events | |
| US9204599B2 (en) | Detection of AAD1 event DAS-40278-9 | |
| AU2011311955B9 (en) | Endpoint TaqMan methods for determining zygosity of cotton comprising Cry 1F event 281-24-236 | |
| US9534260B2 (en) | Materials and methods for detecting the aryloxyalkanoate dioxygenase gene (AAD-12) containing event pDAB4472-1606 in plants | |
| US9243287B2 (en) | Endpoint TaqMan methods for determining zygosity of cotton comprising Cry1Ac event 3006-210-23 | |
| EP2467500B1 (en) | Detection of aad-1 event das-40278-9 | |
| EP2768981B1 (en) | Method to determine zygosity of the fad2 gene in canola using end-point pcr | |
| HK1173194B (en) | Detection of aad-1 event das-40278-9 | |
| BR112012003873B1 (pt) | Método para determinar zigosidade de uma planta de milho compreendendo um transgene de ariloxialcanoato dioxigenase (aad-1) como encontrado no evento de milho 5 das-40278-9, e kit para realizar o referido método |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20180819 |