RU2558218C1 - "vitafit-c" biologically active additive and method for manufacture of "vitafit-c" biologically active additive based on alfalfa extract - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: biologically active food additive manufacture method involves preparation of alfalfa hay vegetal raw materials in an amount of 40-60 wt %, the components and water weighing, preparation of solution of alfalfa hay and water heated to 15-35°C in the tank with addition of an extragent composed of Celloviridin G20kh and orange rind. Then one performs maintenance during 24 hours, the contents transloading into the adiabatic kettle, addition of microelements, extraction at a temperature of 140°C under a pressure of 6×105 Pa during 10 minutes, maintenance during 20 minutes for an extract obtainment. Then one performs the extract drying in the drying oven at a temperature of 55-60°C during no less than 4 hours. The biologically active additive contains alfalfa hay, zinc, cuprum, cobalt, manganese, distilled water, Celloviridin G20kh and orange rind at the following components ratio, wt %: alfalfa hay - 40-60, Celloviridin G20kh - 3-4, orange rind - 0.2-0.4, zinc - 0.03-0.06, manganese - 0.03-0.06, cuprum - 0.0048-0.0064, cobalt - 0.006-0.018, distilled water up to 100.

EFFECT: invention allows to develop a cheaper method for manufacture of a biologically active additive with increased yield of extractive substances.

2 cl, 7 tbl, 3 ex

Description

The invention relates to biotechnology, in particular to biologically active preparations based on plant materials, and can be used in the pharmaceutical, food and cosmetic industries and in veterinary medicine.

This drug can be used as a therapeutic and preventive food or feed supplement.

Plant extracts are currently widely used for the production of various groups of food products, including biologically active food additives, as well as dishes and culinary products in the catering system.

The priority area of these industries is dry plant extracts, given the possibility of maximum preservation of extractable biologically active substances, increase shelf life and the possibility of transportation over long distances.

Various methods are known from the prior art for producing extracts from plant materials, which differ in the nature of the extractant (water, water-alcohol mixture, whey, liquefied gases), physical exposure methods (microwave processing, ultrasound).

One of the richest chlorophyll plants is alfalfa. Eating alfalfa gives a person health, strength and energy to a very old age, while the body's resistance to infections is phenomenal.

The flavonoid content in terms of luteolin is 0.6-1.5%; 0.5% in the plant mass of alfalfa grass (according to the article - Lukmanova K.A., Nasyrov H.M., Kireeva PM, Salikhova L.A., Halikova A.F., Nigmatullin T.G. Experimental and Clinical Evaluation of the Hepatoprotective Effect of Alfalfa Seed Extract - Healthcare of Bashkortostan, N 3-4, 1998, pp. 28-32).

In the organic part of the dry matter of alfalfa, carbohydrates (fiber, BEV) occupy the largest proportion. During the destruction of the lignin-carbohydrate complex, in the process of hydrobarothermic processing of raw materials, hydrolysis of plant tissue occurs with the formation of simple sugars: glucose, sucrose, fructose, etc. In addition, soluble ash substances, vitamins, part of proteins and amino acids up to 15.56 mg go into the aqueous extract; organic acids up to 6.96 g; sugars up to 24.14 g; inorganic chemical compounds up to 27 wt. %; water 12 - 18%

Known biologically active drug "Lucewita" based on alfalfa extract obtained by treating it with an extractant containing a mixture of trace elements salts in the following ratio, mg / kg of plant material: iron - 50.0-300.0, zinc - 50.0-200.0 titanium - 0.5-5.0, copper - 0.5-5.0, cobalt - 0.5-5.0, manganese - 0.5-5.0, chromium - 0.5-1.0 , vanadium - 0.5-2.0, molybdenum - 0.5-5.0 (patent No. 2176918). This biologically active drug was produced by the company “Lutsevita” (Chelyabinsk region, Troitsk) under the name of dietary supplement Lutsevita (Registration No. 004836.Р.643.09.2002).

The disadvantage of Lucevita is the introduction of the trace element titanium into it, which may be necessary for the human body, but there is no literature data on the lack of this chemical compound in the human diet. In addition, the daily human need for this substance has not been established. Titanium is not included in the methodology of rational nutrition, the recommended levels of consumption of food and biologically active substances, approved by the chief state sanitary doctor of the Russian Federation on July 2, 2004 (No. MP2.3.1.1915-04).

The disadvantage of Lutsevita dietary supplement is that it is used as a source of trace elements, but these substances are not regulated in it, but are used only in production technology.

The next drawback is that Lucevita is not a source of amino acids (Table 1) and organic acids (Table 2) with a recommended intake of 2 grams per day [Nutrition. Recommended levels of consumption of food and biologically active substances / V.A. Tutelian, A.K. Baturin, O.A. Vrzhenskaya et al. // Methodology MP2.3.11915-04 of 02.07.2004]. Although on the package it is indicated that Lucevita is used for a deficiency of the above micronutrients of food.

A known method of obtaining an extract from plant materials (nettle leaves, dandelion and burdock roots) according to the patent of the Russian Federation No. 2332225, including extraction with water at a temperature of 95-100 ° C for 45-60 minutes The disadvantage of this method is a single extraction of raw materials with water, increased temperature and duration of extraction, which reduce the stability of biologically active substances, and a single extraction does not allow the optimal yield of extractive substances.

A known method of producing plant extracts for the production of soft drinks, including water-enzymatic treatment of crushed to a particle size of 5-10 mm of plant material for 2 hours at a temperature of 40-45 ° C and a pH of 4.5-5.0. Then, the extraction is carried out with a 20% aqueous-alcohol solution in two stages: stage 1 — extraction process 10-12 hours, stage 2 — 4-6 hours with periodic stirring for 5 minutes every two hours. As an enzyme preparation, cytosemine Px or the combined use of cytosemine Px and protosubtilin G10x (Filonova G.L., Salmanova L.S., Yudakova O.A., Asviyan N.L., Litvinova E.A., Kulikova M.V. Rational technology for processing local plant materials for the production of soft drinks. - M .: TSNIITEI PISCHEPROM, Food industry, series 22. Brewing and soft drinks: overview, 1985, issue 7, 44 pp.).

The disadvantage of this method is the use of alcohol, which, on the one hand, limits the amount of dosing of plant extracts in soft drinks, and on the other hand, alcohol is undesirable in the composition of drinks that are consumed in large quantities by children. In addition, the disadvantage of this method is the duration of the extraction process.

A known method of obtaining a plant extract with a high yield of selenium and biologically active substances according to the patent of the Russian Federation No. 2391875 published on 06/20/2010, the Method includes grinding plant materials (sweet clover, mint, wormwood, pine needles, meadowsweet) to a particle size of 5-8 mm, water -enzymatic extraction of a mixture of raw materials with proteolytic and cellulolytic enzymes at a temperature of 50 ° C for 3 hours.

The disadvantage of this method is the use of a complex of herbs (sweet clover, mint, wormwood, needles, meadowsweet), which in the complex can have an allergic reaction in humans, a single extraction process and a sufficiently large grinding of raw materials (5-8 mm), which does not provide maximum extraction of extractive and antioxidant substances from raw materials.

A known method of obtaining a plant extract (patent No. 2337951). The crushed plant material is insisted at room temperature for at least 2 days in 70% ethanol with a weight ratio of raw materials and alcohol from 1: 4 to 1: 6, then the alcohol content in the extract is reduced to a concentration of 55-35% mainly by distillation under reduced pressure dilute the resulting extract with water to an alcohol concentration of not higher than 1.5% while maintaining the pH of the extract in the range of 3.5-6.5. For long-term storage, a preservative is added to the extract - sodium benzoate or potassium sorbate, the pH of the extract is maintained by adding citric and / or ascorbic acid. This allows you to get the extract with a low alcohol content, but retaining useful properties and usable for a long time, the same as with traditional alcohol tinctures.

The disadvantage of this method is the use of alcohol, which, on the one hand, limits the amount of dosing of plant extracts in soft drinks, and on the other hand, alcohol is undesirable in the composition of drinks that are consumed in large quantities by children. In addition, the disadvantage of this method is the duration of the extraction process, and the preservative sodium benzoate used can provoke asthma and urticaria, therefore it is not recommended for use by children.

A known method of obtaining an oil extract from medicinal raw materials - patent No. 2078577, published 05/10/1997, the Essence of the invention consists in the extraction of freshly collected medicinal plant materials with vegetable oil, and all of its parts, during the maximum accumulation of biologically active substances for at least 20 days at a temperature of 15 -30′C in the ratio 1: (3-5). The technical result consists in a more complete extraction of medicinal substances. The disadvantages of this method include the limitation of the timing of the preparation of the extract by the period of collection of herbs, because the extract is prepared only from freshly picked raw materials. The disadvantage is the need to approach the processing of raw materials to places of its procurement.

A known method of obtaining a dry extract from plant materials (patent No. 2192272). The extraction of the crushed air-dried plant material is carried out by boiling for 10-20 minutes at a mass ratio of plant material and water (1: 5) - (1:20), crushed air-dried brown seaweed in the amount of 25-50 is added to the filtered extract % by weight of the extract, the resulting mixture is stirred until a homogeneous consistency is obtained, the resulting mixture is kept for 0.5-24.0 hours, and the aged mixture is dried at atmospheric pressure and a temperature of 40-85 ° C (using algae as brown seaweed grew kind of kelp or tricks).

One of the analogues of the present invention is a biologically active drug based on alfalfa extract described in the patent for the invention of Russia No. 2145863 obtained by treating alfalfa plant materials with an extractant containing a mixture of trace elements salts of manganese, barium, tin, cobalt, chromium, vanadium, zinc, iron and copper. This drug is produced by NPO Erakond LLC (Republic of Bashkortostan) under the name Erakond dietary supplement. Dietary supplement is registered by the Ministry of Health of the Russian Federation (registration certificate N000150.Р.643.07.98), is entered in the Register of products that have passed state registration (certificate No. 77.99.11.U.9568.10.09). Erakond is recommended to be used as a source of minerals and bioflavonoids - rutin.

The method of obtaining dietary supplements Erakond differs from Lucevita in that the extractant contains trace elements in the following ratio, mg / kg of plant material: manganese - 22.0, cobalt - 4.0, chromium - 1.0, vanadium - 1.8, zinc - 700 , 0, iron - 1030, copper - 1.0, additionally tin - 0.6, selenium - 1.0, barium -13.0. There is no titanium and molybdenum in the extractant. The ratio of plant materials to the extractant is 1: 8. Extracting the mixture is carried out at a temperature of 150 ° C.

The method of obtaining dietary supplements Erakond is as follows. Alfalfa hay is fed to a chopper of plant materials, where it is crushed to 3-5 mm. To prepare the extractant, steam condensate and solutions of trace elements are used. Plant materials and extractant in a ratio of 1: 8 are placed in a mixer and mixed. The temperature of the extractant is 60-80 ° C. Mixing time 20 minutes. The mixing process is carried out using a mixer. For more efficient mixing, the mixer provides pulp circulation using the built-in pump. The pulp circulation process lasts until the total mixing time has elapsed. The finished pulp from the mixer is pumped to a reactor equipped with a pressure, temperature and safety valve. After loading the pulps, the reactor begins to be heated with steam at a pressure of 6 × 10 ⁵ Pa to a temperature of 140-150 ° C for 90 minutes. The extract enters the tank due to the difference in pressure in the reactor and the tank. After centrifugal purification of suspended particles from the container, the extract enters the evaporator. To obtain a powdery and plastic form of the preparation, a 40% concentrated extract is fed to a spray drying plant, where air is supplied with a counter-fluid at a temperature of 55-60 ° C. The dried product is collected in receiving containers.

At the same time, the following components, wt. %: alfalfa hay - 12.5, manganese - 0.0022, barium - 0.0013, tin - 0.000006, cobalt - 0.0004, chromium - 0.0001, vanadium - 0.00018, zinc - 0.07 , iron - 0.1030, selenium - 0.0001, copper - 0.0001, distilled water up to 100%, or salts of trace elements are taken in the following ratio, mg / kg of plant material: manganese - 22.0, barium - 13.0 , tin - 0.6, cobalt - 4.0, chromium - 1.0, vanadium - 1.8, zinc - 700.0, iron - 1030.0, selenium - 1.0, copper - 1.0, at the ratio of plant materials: extractant 1: 8.

A powder or dark brown plastic mass is obtained, which is then packaged, packaged, stored at a temperature of 0-12 ° C, with an air humidity of not more than 75%.

A significant disadvantage of the method of obtaining dietary supplements Erakond is the use in the formulation of a small amount of alfalfa hay 12.5 wt. % or in the ratio of plant materials: extractant - 1: 8, which is not enough for bioflavonoids to exit from alfalfa to the extract. Thus, the formulation used leads to a low concentration of biologically active substances, in particular flavonoids, in the resulting dietary supplement.

It should be noted that dietary supplements Erakond declared as a source of bioflavonoids, in particular rutin, which is its drawback, because the content of rutin in it is 3.5 mg per 1 g of dietary supplement with an adequate level of human consumption of 30 mg per day, and the upper allowable level of consumption is 100 mg [Rational nutrition. Recommended levels of intake of food and biologically active substances. / V.A. Tutelian, A.K. Baturin, O.A. Vrzhenskaya et al. // Methodology MP2.3.11915-04 of 02.07.2004].

For oral administration of dietary supplement Erakond, it is recommended to add 100 ml of warm boiled water to 20 g of dietary supplement Erakond, mix thoroughly and consume 1-2 teaspoons 2-3 times a day.

Therefore, even with the calculation of the maximum dose of 6 teaspoons (30 ml) per day, rutin enters the human body in the amount of 6.33 mg (21.1% of the daily requirement) at a rate of 30 mg per day [Rational nutrition. Recommended levels of intake of food and biologically active substances. / V.A. Tutelian, A.K. Baturin, O.A. Vrzhenskaya et al. // Methodology MP2.3.11915-04 of 02.07.2004]. [Balanced diet. Recommended levels of intake of food and biologically active substances. / V.A. Tutelian, A.K. Baturin, O.A. Vrzhenskaya et al. // Methodology MP2.3.11915-04 of 02.07.2004]. When using Erakond dietary supplement in capsules (2 capsules per 50 ml of water), which is 1 g of Erakond dietary supplement, rutin enters the human body in an amount of 3.5 mg (11.6% of the daily requirement).

When using Erakond dietary supplements even at a dose of 4 g per day (2 incomplete teaspoons) during meals, dissolving in water, tea, milk, juice, rutin enters the human body in an amount of 14 mg (46.7% of the daily requirement) ( Register of products that have passed state registration, p. 4).

Therefore, the disadvantage of dietary supplement Erakond is that it is a weak source of bioflavonoids in recommended doses, because any dietary supplement is recommended to be used in order to fill the deficit of the regulated nutrients in it in the human diet in amounts of 30-100% of the physiological needs of a person.

The disadvantage of Heraconda is the presence of tin and barium salts, which are not necessary for the human body [Method MP2.3.11915-04 of 02.07.2004].

It should be noted that Erakond is considered a source of minerals: potassium, calcium, magnesium and zinc (Register of products that have passed state registration).

The potassium content in it is 50.0-60.0 mg / g with a normal intake for an adult per day of 2500-3500 mg / g; calcium 25.0-50.0 mg / g with a consumption rate of 1250-2500 mg / g; magnesium 10.0-15.0 mg / g with a consumption rate of 400-800 mg / g; zinc 1.0-2.0 mg / g with a consumption rate of 12.0-40.0 mg / g ((No. MP2.3.1.1915-04). Therefore, dietary supplements Erakond in recommended doses (1-4 g) provides in potassium by 2.4-9.6%, in calcium - 2-8%, in magnesium - 2.5-10% and zinc - 8.3-33.2% of the daily needs of an adult.

Thus, Herakond cannot be used as a source of the above minerals.

The closest technical solution, selected as a prototype, is the patent for the invention of Russia No. 2435455, published 10.12.02011, the Method for producing biologically active food additives "Eramin" and biologically active additive "Eramin".

A method of obtaining a biologically active additive includes preparing plant materials, weighing the components of the formulation, preparing a solution from a dry mixture of alfalfa hay, trace elements and distilled water, extracting in time, at a pressure of 6 × 10 ⁵ Pa, drying the extract in an oven at a temperature of 55-60 ° C, lasting 4 hours, prepare a solution from a dry mixture of alfalfa hay and trace elements, with alfalfa hay content of 40-60 wt. %, extracted at a temperature of 180-190 ° C for 5-6 hours.

The dietary supplement contains alfalfa hay, a molybdenum-containing element, an iron-containing element, a zinc-containing element, a copper-containing element, a cobalt-containing element, a chromium-containing element, a vanadium-containing element, distilled water, and metal salts: iron sulfate 7-hydrous, as metal-containing elements. 7-water, copper sulfate 5-water, chromium (3) sulfate 6-water, cobalt sulfate 7-water, manganese sulfate 5-water, ammonium vanadium acid, as as a molybdenum-containing element, it contains ammonium molybdenum acid, regulated by the average daily consumption rate in the following ratio of components, mass. %:

alfalfa hay 40-60 iron sulfate 7-water 0.350-0.450 zinc sulfate 7-water 0.08-0.09 5-manganese sulfate 0.045-0.065 5-hydrous copper sulfate 0.001-0.004 ammonium molybdenum acid 0,0006-0,0012 ammonium vanadium 0,0006-0,0012 cobalt sulfate 7-water 0.0015-0.0025 chromium (3) sulfate 6-water 0,0006-0,0012 distilled water to one hundred

BAA Eramin is a dark brown plastic substance. It hardens upon drying. The taste is bitter sour. Soluble in water.

The objective of the invention is to develop a cheaper method for producing plant extracts with increased 1.5-2 times the yield of extractive and antioxidant substances from plant materials.

This technical result is achieved by the fact that in the method of producing a biologically active additive, including the preparation of plant materials - alfalfa hay 40-60 mass. %, weighing components and water, preparing a solution of alfalfa hay and water with the addition of trace elements, extraction in time, at a temperature above 100 ° C, pressure 6 × 10 ⁵ Pa, drying the extract in an oven at a temperature of 55-60 ° C, with a duration of at least 4 hours, according to the invention, a solution is prepared from alfalfa hay and drinking water heated to 15-35 ° C in a tank with the addition of extractant-1 in the composition of G20X celloviridine and orange peel, incubated for 24 hours, after 24 hours the contents are transferred to adiabatic boiler add microelement s, extracted at 140 ° C for 10 minutes, allowed to stand for 20 minutes to obtain the extract.

This technical result is achieved by the fact that the biologically active additive obtained by the method according to claim 1, characterized in that it contains alfalfa hay, zinc, copper, cobalt, manganese, distilled water, as an extractant - G20X cellovirdine and orange zest in the following ratio components, mass. %:

alfalfa hay 40-60 cellovirdine G20X    3-4 orange peel 0.2-0.4 zinc 0.03-0.06 manganese 0.03-0.06 copper                                                 0.0048-0.0064 cobalt 0.006-0.018 distilled water to one hundred

Due to the fact that in the method for producing a biologically active additive, a solution is prepared from alfalfa hay and drinking water heated to 15-35 ° C in a tank with the addition of extractant-1 in the composition of G20X celloviridine and orange peel, withstand 24 hours, after 24 hours the contents are overloaded trace elements are added to the adiabatic boiler, extracted at 140 ° C for 10 minutes, kept for 20 minutes to obtain an extract, and the most biologically active additive contains alfalfa hay, zinc, copper, cobalt, manganese, distilled water s, as extractant - cellovirdine G20X and orange peel in the following ratio of components, mass. %:

alfalfa hay 40-60 cellovirdine G20X 3-4 orange peel 0.2-0.4 zinc 0.03-0.06 manganese 0.03-0.06 copper 0.0048-0.0064 cobalt 0.006-0.018 distilled water to one hundred

the content of immunoglobulins with antioxidant properties increases (the activity of the enzyme catalase, the content of ceruloplasmin and immunoglobulins).

The increased yield of extractive and antioxidant substances from alfalfa in the claimed invention is achieved by increasing the mass fraction of alfalfa hay and the introduction of the enzyme preparation celloviridine G20X, which contributes to a deeper destruction of both cell walls and individual plant polysaccharides: cellulose, glucan, xylan, hemicellulose, araban and other non-starchy polysaccharides, and hence the release of extractive substances.

All the technology and the recipe for VITAFIT-S dietary supplement with the stated technical result has been selected and strictly verified by the applicant in detail by changing the technological parameters (extraction temperature, time of the process steps) and numerous test batches. Thanks to the heat treatment of plant materials and such a selection of components in the composition, the necessary technical result is achieved.

Comparative analysis with the prototype allows us to conclude that the claimed technology differs from the known for its parameters and sequence of introduction of the components, and the formulation differs from the known new quantitative composition of the components.

The set of essential features of the claimed technical solution, technology and composition do not follow explicitly from the studied prior art, i.e. meet the criteria of "novelty" and "inventive step".

The declared dietary supplement VITAFIT-S will find wide application in the food industry, i.e. composition and technology are industrially applicable.

The invention is illustrated by examples.

Example 1

The method of obtaining dietary supplements "VITAFIT-S" is based on hydrobarothermic processing of plant materials.

Plant material - alfalfa hay (GOST 27978-88), collected in the budding phase (crude protein content of at least 17% in dry matter and metabolic energy of at least 9.6 MJ), washed with drinking water (GOST 51232-98) in order to remove pollution. Grind to a straw cutting of 1-2 cm. Grinding is necessary to facilitate the penetration of water into the thickness of a material having a cellular structure. In addition, grinding is also necessary to increase the contact surface of water with particles of material, since the amount of extracted substances is directly dependent on the surface of diffusion.

Prepare a solution in the tank in the following ratio of components, mass. %: alfalfa hay - 40.0 with the addition of an extractant in the composition of celloviridine G20X - 3.0, orange peel - 0.2 (powder) and drinking water heated to 15-35 ° C - 56.7392, incubated 24 hours, after 24 hours, the contents are loaded into an adiabatic boiler, trace elements are added: zinc - 0.03, manganese - 0.03, copper - 0.0048, cobalt - 0.006, extracted at a temperature of 140 ° C, a pressure of 6 × 10 ⁵ Pa for 10 minutes, kept for 20 minutes at a pressure of 6 × 10 ⁵ Pa, the autoclave is depressurized, the extract is poured into a separator. After separation, the extract is pumped to the accumulator, from which it is fed to a vacuum evaporator, where water is extracted from the extract to a dry solids content of 50%.

The resulting extract is continued to evaporate in an oven at a temperature of 55 ° C until a plasticizing mass forms over the entire thickness of the extract poured into the baking sheet. The extract is dried in an oven at a temperature of 55-60 ° C, lasting at least 4 hours.

Example 2

The method of obtaining dietary supplements "VITAFIT-S" is based on hydrobarothermic processing of plant materials.

Plant material - alfalfa hay (GOST 27978-88), collected in the budding phase (crude protein content of at least 17% in dry matter and metabolic energy of at least 9.6 MJ), washed with drinking water (GOST 51232-98) in order to remove pollution. Grind to a straw cutting of 1-2 cm. Grinding is necessary to facilitate the penetration of water into the thickness of a material having a cellular structure. In addition, grinding is also necessary to increase the contact surface of water with particles of material, since the amount of extracted substances is directly dependent on the surface of diffusion.

Prepare a solution in the tank in the following ratio of components, mass. %: alfalfa hay - 60.0 with the addition of an extractant in the composition of celloviridine G20X - 4.0, orange peel - 0.4 (powder) and drinking water heated to 15-35 ° C - 35.4556, incubated 24 hours, after 24 hours, the contents are reloaded into an adiabatic boiler, trace elements are added: zinc - 0.06, manganese - 0.06, copper - 0.0064, cobalt - 0.018 are extracted at a temperature of 140 ° C, a pressure of 6 × 10 ⁵ Pa for 10 minutes, withstand for 20 minutes at a pressure of 6 × 10 ⁵ Pa, the autoclave is depressurized, the extract is poured into a separator. After separation, the extract is pumped to the accumulator, from which it is fed to a vacuum evaporator, where water is extracted from the extract to a dry solids content of 50%.

The resulting extract is continued to evaporate in an oven at a temperature of 55 ° C until a plasticizing mass forms over the entire thickness of the extract poured into the baking sheet. The extract is dried in an oven at a temperature of 55-60 ° C, lasting at least 4 hours.

Example 3

The method of obtaining dietary supplements "VITAFIT-S" is based on hydrobarothermic processing of plant materials.

Plant material - alfalfa hay (GOST 27978-88), collected in the budding phase (crude protein content of at least 17% in dry matter and metabolic energy of at least 9.6 MJ), washed with drinking water (GOST 51232-98) in order to remove pollution. Grind to a straw cutting of 1-2 cm. Grinding is necessary to facilitate the penetration of water into the thickness of a material having a cellular structure. In addition, grinding is also necessary to increase the contact surface of water with particles of material, since the amount of extracted substances is directly dependent on the surface of diffusion.

Prepare a solution in the tank in the following ratio of components, mass. %: alfalfa hay - 50.0 with the addition of extractant-1 in the composition of celloviridine G20X - 3.5, orange peel - 0.3 (powder) and drinking water heated to 15-35 ° C - 46.0924, incubated for 24 hours, after 24 hours, the contents are loaded into an adiabatic boiler, trace elements are added: zinc - 0.045, manganese - 0.045, copper - 0.0056, cobalt - 0.012, extracted at a temperature of 140 ° C, a pressure of 6 × 10 ⁵ Pa for 10 minutes, kept in for 20 minutes at a pressure of 6 × 10 ⁵ Pa, the autoclave is depressurized, the extract is poured into a separator. After separation, the extract is pumped to the accumulator, from which it is fed to a vacuum evaporator, where water is extracted from the extract to a dry solids content of 50%.

The resulting extract is continued to evaporate in an oven at a temperature of 55 ° C until a plasticizing mass forms over the entire thickness of the extract poured into the baking sheet. The extract is dried in an oven at a temperature of 55-60 ° C, lasting at least 4 hours.

Orange peel has an antiseptic effect against bacteria (MAFAM), fungi and yeast. The number of MAFAM is reduced by 57-71%, molds - by 30-44% compared with the control.

Cellovirdine G20X - an enzyme preparation is allowed and is used in bakery (grain bread). It contains a complex of enzymes - carbohydrase of the strain trichoderma reesei (viride), capable of deep destruction of both cell walls and individual plant polysaccharides: cellulose, glucan, xylan, hemicellulose, araban and other non-starch polysaccharides.

The number of trace elements introduced into the composition and their ratio is established taking into account the daily human needs and their presence in alfalfa hay.

Biogenic microelements introduced into the BAA formula at the stage of solution preparation form organometallic complexes with a large number of metals with hexoses and amino acids by coordination through a carboxyl group.

The introduction of cobalt in the extractant is due to its participation in the processes of blocking and disposal of toxic elements, participation in enzymatic processes and the formation of thyroid hormones, normalization of mineral, protein, carbohydrate and fat metabolism.

Manganese helps to prevent degenerative changes in the joints, reduce autoimmune and allergic reactions, normal functioning of muscle tissue, remove inflammatory processes in it, reducing treatment time. Manganese enhances metabolic processes with less formation of compounds that adversely affect the body.

Copper is involved in bone mineralization processes, in the production of thyroid hormones, and has anti-inflammatory and immunostimulating properties. Copper is necessary for the normal development of the skeleton. With copper deficiency, collagen synthesis is disrupted and soluble collagen (tropocollagen) predominates in the bone tissue, which is accompanied by increased fragility and deformation of bones. A lack of copper leads to the development of osteodystrophy and rickets.

Zinc is involved in bone calcification, protein and carbohydrate metabolism, and boosts immunity. Zinc deficiency is accompanied by regidity, joint swelling and lameness, and a decrease in the size and strength of the femur. Zinc deficiency inhibits alkaline phosphatase in chondrocytes of the epiphyseal cartilage, which is the main biochemical defect in the development of bone tissue.

The proposed method for producing the extract is environmentally friendly, as its production uses easily sterilized industrial equipment (abiotic boiler, steam generator, filter, vacuum-evaporator), approved for use in the manufacture of medicines and biologically active food additives.

Supplement "VITAFIT-S" is a dark brown plastic substance. It hardens upon drying. The taste is bitter sour. Soluble in water.

Supplements "VITAFIT-S" are produced in the form of a plastic substance of 1 g in bags of plastic film in the form of tablets of 1 g, 5-10 tablets per pack.

Industrial production of biologically active additives is carried out at the company UralVetAgro Plus LLC (Troitsk, Chelyabinsk Region).

Supplement "VITAFIT-S" has a strong antioxidant activity. First of all, this is due to the high content of bioflavonoids, in particular luteolin, which stimulates the enzymatic and non-enzymatic parts of the antioxidant system of the body.

In every human cell, there is a protective mechanism that prevents excessive accumulation of lipid peroxidation products - this is an antioxidant system. According to modern concepts, this system consists of two links:

- enzymatic, represented by oxyreductase (glutathione reductase, etc.) and antiperoxid (catalase (K), superoxide dismutase, etc.) and enzymes;

- non-enzymatic, represented by water-soluble vitamins, proteins and other compounds.

Luteolin prevents abnormalities in DNA molecules, enzymes, polyunsaturated fatty acids and other compounds through radical-radical interactions.

Luteolin enhances the activity of oxidoreductase (glutathione reductase) and antiperoxidase enzymes (catalase), increases the content of immunoglobulins and ceruloplasmin (CP) proteins, which protect lipid membranes from peroxidation and have the activity of oxidoreductase enzymes ferroxidase, glutanthtide oxidase, superglutantion reductase. Using pairs of copper (II) ions, the CP binds the superoxide radical ion and restores oxygen to water.

In connection with the foregoing, the evaluation of the antioxidant activity of dietary supplements of Lucevita, dietary supplements Erakond, dietary supplements Eramin and dietary supplements "VITAFIT-S" was carried out according to the following indicators: enzyme catalase (K) and ceruloplasmin (CP).

To study the effect of dietary supplements on the antioxidant activity of blood, 5 groups of people (students of the Ural State Academy of Veterinary Medicine (UGAVM) aged 18-20 years old) were formed by 5 students in each group. Group 1 (control) - students in this group did not accept dietary supplements. People of the second experimental group took Lucevit dietary supplements 1 tablet (1 g) twice a day for 20 days, students of the third group took Erakond dietary supplements, after dissolving the dietary supplement powder (4 g) in water, twice a day for 30 days , according to the Register of Products that passed state registration, page 4. People of the fourth group took Eramin dietary supplement per tablet (1 g) twice a day for 20 days, and students of group 5 took VITAFIT-S dietary supplement per tablet (1 g) twice a day for 30 days.

Activity (K) was determined by the conventional method based on the decomposition of hydrogen peroxide by catalase and the determination of the light absorption of the complex with ammonium molybdate at a wavelength of 410 nm.

Determination (CP) in blood serum - according to the modified method C.V. Bestuzheva, V.G. Kolb (1976).

The studies were carried out on the basis of the interdepartmental laboratory of the UGAVM.

The obtained materials were processed by the method of variation statistics using Student's criterion and the Biostat computer program.

Table 1 presents the effect of dietary supplements on human antioxidant protection — the activity of the catalase enzyme and the content of ceruloplasmin in the blood serum of people in the control group and those who took dietary supplements.

From the data in table 1 it can be seen that, against the background of the use of VITAFIT-S dietary supplement, the activity of catalase and the content of ceruloplasmin in the blood of people of the fifth group significantly increased by 22.6% and 27.0%, in the fourth by 19.8% and 24, respectively. 6%, in the third - 11.4% and 8.4%, and in the second by 8.4% and 11.4%. In the blood of people in the control group, there were no significant changes in the activity of catalase and ceruloplasmin.

Thus, VITAFIT-S dietary supplement is more than 2 times more effective in antioxidant protection of the human body than its analogues - Lutsevita dietary supplement, Erakond dietary supplement and Eramin dietary supplement.

Given the different nature of the compounds exhibiting antioxidant properties, the following indicators were used to determine the effect of Lutsevit dietary supplements, Erakond dietary supplements, Eramin dietary supplements and VITAFIT-S dietary supplements on the antioxidant defense of the body: it is these immunoglobulins that are proteins with pronounced antioxidant properties.

Immunoglobulins were determined by the method of radial immunodiffusion according to A.A. Tikhomirova (1977).

Table 2 presents data on the percentage of immunoglobulins A, M, G in the blood serum of people in the control group and taking dietary supplements.

From the data of table 2 it follows that the number of immunoglobulins A, M, G after taking the course of dietary supplements "VITAFIT-S" in the blood serum of people significantly increased by 25.0%, 27.25% and 35.6%, dietary supplements Eramin, respectively , 7%, 15.0% and 33.1%, dietary supplements Erakond the content of immunoglobulins A, M, G increased by 12.5%, 11.4% and 26.2% and dietary supplements of Lucevita in the diet of people increased the content of immunoglobulin A by 7.9%, immunoglobulin M - 8.1% and G - 12.0%.

Thus, the use of dietary supplements of Lucevita, Erakonda, Eramina and VITAFIT-S increases the content of immunoglobulins with antioxidant properties, while the greatest effect is achieved when using VITAFIT-S dietary supplements.

Based on the studies, we can conclude that the technical result of the application, characterized by an increase in the content of bioflavonoids in dietary supplements, allows you to strengthen the antioxidant system of the human body (activity of the catalase enzyme, the content of ceruloplasmin and immunoglobulins), which leads to the promising application of the method of producing biologically active supplement "VITAFIT-S" in the food industry, providing the maximum possible yield of bioflavonoids from plant materials in the resulting dietary supplement.

In addition, an assessment was made of the antioxidant activity of dietary supplements "VITAFIT-S" in animal husbandry.

In order to solve the tasks we set, on the basis of LLC Demetra, Uvelsky district of the Chelyabinsk region, in the period from April to September 2013, we conducted scientific and economic experience on black-and-white calves, selected in groups taking into account age, live weight, origin and sex. The experimental design is presented in table 3.

During the entire scientific and economic experiment, all experimental animals received a diet in accordance with a detailed system of normalized feeding. Supplement "VITAFIT-S" in the studied dosage was individually fed to each calf with milk feed during morning feeding. Feeding of calves during the dairy period was carried out in accordance with the feeding scheme adopted by the farm. The change in calf live weight was monitored by monthly weighing of each head separately, followed by calculation of the absolute and average daily gain in live weight. Hematological studies were carried out three times: in the preparatory period, as well as when calves reached the age of 4- and 6-month-old according to generally accepted methods. The resulting material was processed biometrically on a personal computer with software.

From the changes in the calves' live weight presented in Table 4 for the period of scientific and economic experience (X ± S _x , n = 20) of the periodic weighing data of the calves of the control and experimental groups, it can be seen that when setting up scientific and economic experience, their live weight in the groups did not have significant differences and varied from 30.30 kg in animals of groups I and III to 30.70 kg in the II experimental group. Feeding the calves from the first day of the milk period of VITAFIT-C dietary supplement in the studied dosages had a definite effect on their growth and development.

As a result, at 6 months of age, their live weight was 155.70 kg in group I, 162.20 kg in group II, 164.50 kg in group III and 163.40 kg in group IV, that is, animals of the experimental groups they exceeded the analogues of the control group by 6.00 kg in the II group, by 8.75 kg in the III and by 7.55 kg in the IV group. The calves of the experimental groups, respectively, had an average daily gain in live weight of 718 g, 733 g and 727 g, which was 4.8% (P≤0.01), 7.0% (P≤0.001) and 6.0% (P≤ 0.001) exceeded the calves of the control group, in which it was at the level of 686 g. Moreover, the relative increase in live weight during the period of the experiment was 134.93% in group I, 136.32% in group II, 137.80% in group III and 137.27% - in group IV.

Hematological studies largely allow you to control the physiological state of animals, the state of their immune system and the course of metabolic processes in the body. The data of a periodic blood test of experimental calves during the period of scientific and economic experience are presented in table 5 "Morphological composition of the blood of calves (X ± S _x , n = 5)".

In the preparatory period, in experimental animals of the control and experimental groups, no significant differences were observed in the studied indicators of the morphological composition of the blood. The average hemoglobin content in whole blood ranged from 100.4 to 103.8 g / l, erythrocytes from 4.58 to 4.81 million / μl, and white blood cells from 6.13 to 6.38 thousand / μl. At the age of 4 months, the metabolism in the body of the calves of the experimental groups changed in comparison with the control, as evidenced by the analyzed parameters. So, if in the I control group the hemoglobin content was at the level of 103.2 g / l, then in the II group its amount increased by 2.8 g / l, in the III - by 8.5 (P≤0.05) and in Group IV - only 4.0 g / l. An increase in redox processes in the animals of the experimental groups is evidenced by an increase in red blood cells by 11.4% in group II, by 17.8% in group III (P≤0.05) and by 9.7% in group IV in comparison with group I, in which this indicator was 4.72 million / μl. Similar changes are observed in the number of leukocytes in the whole blood of experimental animals. Their content in the blood of calves of the experimental groups, respectively, increased by 1.2%, 4.0% and 1.4%.

Repeated studies at 6 months of age showed that the calves of the control and experimental groups observed a similar pattern as in the previous age period. This indicates an increased metabolism in the body against the background of the use of dietary supplement "VTAFIT-S" and its use in animal husbandry.

An assessment was also made of the antioxidant activity of dietary supplements "VITAFIT-S" in pig breeding.

A study of the influence of VITAFIT-S dietary supplement on the organism of weaned piglets and repair young animals was carried out in three scientific and economic experiments and one scientific experiment. Table 6 shows the experimental design.

In all experiments, the daily norm of dietary supplement VITAFIT-S was corrected with a change in the live weight of animals.

The use of VITAFIT-S dietary supplement for newborn pigs made it possible to obtain an average daily gain in live weight of 2.6% over the period of suction, and their safety by 3.3% higher than in the control group. At the same time, the cost of feed for growing one piglet is 60.2 feed, unit, or 98.0%.

Table 7 shows the changes in live weight and the safety of weaned piglets over the period of the experiment (M ± m).

Blood tests of sucking piglets showed that dietary supplement VITAFIT-S has an erythropoietic effect, i.e. the amount of hemoglobin increased by 5.7% and red blood cells by 8.3%. In addition, the total protein content increased by 5.5-30.3%, total lipids by 32.0-43.6%, calcium by 19.2%, phosphorus 8.4-9.5% compared with the control group.

The use of VITAFIT-S dietary supplement as an adaptogen showed that in the diet of weaned piglets the best effect was obtained from a high dosage of the drug - 75 mg / kg body weight. At the same time, 100% safety of the livestock in the group was observed, and the average daily increase was 60 g, or 20% higher compared to the control group. The dosage test of dietary supplements "VITAFIT-S" 50 mg / kg live weight ensures 100% safety of the livestock, but the increase in live weight was only 4.7% higher, and similar indicators when feeding the drug 25 mg / kg made it possible to obtain 94.7% safety and 307 g of daily average growth, which is 2.3% higher than in the control.

Thus, the effectiveness of the use of VITAFIT-S dietary supplements in the dose of 75 mg / kg live weight in the diets of weaned pigs allows reducing the cost of obtaining 1 kg of live weight gain of 0.6 kg of feed units, and the payment for feed increases by 4.8 -14.1% in relation to the control group.

Thus, “Vitafit-S” can be used as dietary supplement in the amount of 50 mg per kg of human body weight, in the diets of cattle at a dose of 100 mg and pigs 50 mg / kg of live weight, which helps to normalize the imbalance of minerals in the body, increase defenses and normalize metabolism.

Claims (2)

1. A method of obtaining a biologically active additive, including the preparation of plant materials of alfalfa hay 40-60 wt. %, weighing components and water, preparing a solution of alfalfa hay and water with the addition of trace elements, extraction at a pressure of 6 × 10 ⁵ Pa, drying the extract in an oven at a temperature of 55-60 ° C, lasting at least 4 hours, characterized in that a solution is prepared from alfalfa hay and drinking water heated to 15-35 ° C in a tank with the addition of an extractant in the composition of G20X celloviridine and orange peel, incubated for 24 hours, after 24 hours the contents are transferred to an adiabatic boiler, trace elements are added, extracted at a temperature of 140 ° C for 10 minutes, incubated for 20 minutes to obtain the extract. 2. The dietary supplement obtained by the method according to claim 1, characterized in that it contains alfalfa hay, zinc, copper, cobalt, manganese, distilled water, as an extractant, celloviridine G20X and orange peel in the following ratio of components, wt. %:
alfalfa hay 40-60 celloviridin G20X 3-4 orange peel 0.2-0.4 zinc 0.03-0.06 manganese 0.03-0.06 copper 0.0048-0.0064 cobalt 0.006-0.018 distilled water to one hundred