RU2641907C1 - Bioactive fodder additive for agricultural animals and poultry - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: fodder additive contains dead bees, drone brood at different stages of development, powder of Jerusalem artichoke tubers, herbal flour, and an activated mineral sorbent - activated flask. All components are taken at a certain ratio.

EFFECT: feeding with the fodder additive stimulates metabolism, the immune system and the antioxidant system of the body, reduces morbidity, improves digestibility of fodders, stimulates the growth and development of young animals, increases their safety and body resistance to agents of physical, chemical and biological nature, providing the sorption-detoxication, antiradiation and anti-infectious effect by means of binding, neutralisation and excretion of radiotoxins, radionuclides, ecotoxins, mycotoxins.

2 cl, 1 tbl, 8 ex

Description

The invention relates to feed production and can be used for feed additives that stimulate all body systems of farm animals and birds.

Known feed additive for farm animals and birds containing sorbent and stimulant. It is used as a plant stimulator of the body under stressful conditions and contains oregano, St. John's wort, coltsfoot, plantain, celandine, sweet clover, wormwood, yarrow, chamomile, pharmacy, dioica nettle (RF Patent No. 2344187, IPC А23К 1/14, 1 / 16). As a sorbent in a feed additive, flasks were used - natural minerals of opalkristobolite rock.

The disadvantage of a feed additive is the use of a significant number (11 types) of valuable medicinal herbs, which only in such a complex combination and number of components provide a wide range of biological effects.

There is a method of obtaining a feed additive from drones larvae and dead bees (1: 1), including collecting drones and dead bees larvae, homogenizing them, mixing with 40% alcohol 10: 1, freezing (-4 ° C) for 42 hours, thawing and subsequent addition of artificial gastric juice, consisting of 3% pepsin, 1% hydrochloric acid, water, stirring, incubation in a thermostat at 120 ° C for 1 hour, then at 39 ° C for 24 hours, straining, autoclaving and packaging (Patent RU No. 2346457, IPC A23K 1/00, publ. 02.20.2009).

The disadvantage is the high rate of introduction of scarce apiproduct - drones larvae (1: 1), complex manufacturing technology, the absence of plant-based biologically active substances and the impossibility of long-term storage.

Known is a feed additive for poultry farming and a method of growing birds containing a complex of biologically active substances based on biomass and culture fluid of the fungus Medusomyces Gisevii Lindau mixed with the probiotic Bifidumbacterin cultivated in a chitin-containing nutrient medium, where bee death powder is used as a source of chitin (Patent RF №2574691 IPC A23K 1/16, publ. 02/10/2016)

This natural biologically active supplement contains a complex of valuable biological substances that have a beneficial effect on the body, however, it has a complex composition and a long production process: 5-day cultivation of tea mushroom in the presence of bee death, fractionation of the product into the culture fluid and biomass, drying, homogenization biomass, obtaining an extract of bark of pine and aspen, the use of two types of sorbents (flask and bentonite). In addition, it is intended for poultry farming and does not remove heavy metals and the long-lived strontium-90 radioisotope.

Also known is a natural biologically active feed additive "Vita-Forte M" containing propolis, bee bread, dressing, bee mortar, brood drone at different stages of development, grass meal, coniferous flour and bentonite (RF Patent No. 2522339, IPC A23K 1/16, A23K 1/100, publ. 07/10/2014).

This natural biologically active supplement that is closest to the one proposed, although it contains a complex of valuable biological substances that have a beneficial effect on the animal’s body, this supplement does not remove mycotoxins, in addition, it has a complex composition, including a significant amount of valuable and deficient substances, such such as propolis, bee bread, pollen, drone brood, which leads to its cost increase, and the presence of blood flour in the composition provides for thorough sanitary and epidemiological control for the exclusion of product intamination by the causative agent of rabies and other pathogens of infectious diseases of humans and animals.

When creating the invention, the task was to obtain a biologically active feed additive with enhanced decorpor and detoxifying effects while maintaining biological activity while reducing its cost and simplifying the technology for its production.

This is achieved by the fact that an activated sorbent fraction - flask and Jerusalem artichoke tubers powder is additionally introduced into a biologically active feed additive containing bee morbidity, drone brood at different stages of development, grass flour, in the following ratio of components, masses. %: bee morbidity - 8-10; drone brood at different stages of development - 2.5-3.0; grass meal - 13.0-15.0; activated flask - 2.5-3.0 and Jerusalem artichoke tubers powder - the rest. In addition, a flask treated with hydrochloric acid followed by fractionation of particles (0.0006-0.0009 mm) was used as an activated flask.

Distinctive features of the proposed feed additive is the introduction of a pectin-containing herbal preparation - Jerusalem artichoke powder using a purified (activated) fraction of the mineral sorbent - flask taken in certain proportions of components, which made it possible to preserve adaptogenic, antibacterial, immunostimulating, metabolic, enterosorption with respect to mycotoxins, radiation protective decorporizing with respect to long-lived radionuclides ( ¹³⁷ Cs and ⁹⁰ Sr) properties, with high efficiency feed leniya, weight gain and safety of young animals, as well as reduce its cost and simplify the technology of production.

Introduction to a biologically active feed supplement of dead bees containing honey, bee venom, propolis, bee bread, pollen and wax - as a source of chitin and chitosan, trace elements, provides antibacterial, antioxidant, adaptogenic properties; drone brood - as a source of essential amino acids, fatty acids, protein, vitamins, hormones; Jerusalem artichoke - as a source of pectin, inulin, fructose can improve radioprotective properties; herbal flour - as an additional source of vitamins, minerals, amino acids, an activator of reproductive function and a stimulator of metabolism; highly dispersed activated fraction of the flask - as an adsorbent of toxicants, radiotoxins and radioisotopes of various groups (osteotropic - ⁹⁰ Sr, muscular-hepatic - ¹³⁷ Cs), and the interacting components all together make it possible to obtain a valuable biologically active feed additive in animal and bird food.

Apiproduct - bee subpestilence contains honey, bee venom, wax, propolis, perga, chitin, fat, protein, hemolymph, carbohydrates, trace elements, enzymes, vitamins, hormones (see St. Nemtseva S.V. et al. Chitin content in the body honey bees // Beekeeping. - 2001 - No. 5. - S. 50-51).

Drone brood is the strongest biostimulating agent, not inferior in composition to royal jelly. It contains proteins, fats, sugars, minerals (Na, Ca, Mn, Cu, Zn, Mg, etc.), vitamins (Δ-tocopherol, β +, j, α-tocopherol; vitamins of group B (B ₁ , B ₂ , B ₃ , B ₅ , B ₆ ); β-carotene, etc.), natural sex hormones: testosterone, progesterone, prolactin, estradiol; bile acids, decenoic acids, sulfhydryl groups and other biologically active substances (see IA Prokhoda article “Obtaining supplementation from bee larvae // Beekeeping. - 2009. - No. 8. - P. 49-50).

Jerusalem artichoke contains pectins, mineral salts, proteins, carbohydrates, many vitamins (groups A, B, C, D, E), inulin. In terms of carbohydrates, organic acids, proteins, vitamins and other biologically active substances, it surpasses many feeds and root crops. The inulin and pectin contained in Jerusalem artichoke contribute to the elimination of many toxicants from the body, activation of the gastrointestinal tract, stimulates the normal intestinal microflora (bifidobacteria, lactobacilli), inhibits the penetration and development of pathogenic and conditionally pathogenic bacteria, parasites in the body, and removes heavy metals from the body and radionuclides, neutralizes radiotoxins and toxins, has a radioprotective effect (see the book. "Biological Enceclopedic Dictionary" - M .: Sov. Encyclopedia, 1989).

Jerusalem artichoke tubers contain up to 3% protein, mineral salts (potassium - 421 mg / 100 g of feed, calcium - 12 mg / 100 g, magnesium - 23 mg / 100 g, sodium - 6 mg / 100 g, phosphorus - 57 mg / 100 g, iron - 0.71 mg / 100 g, manganese - 0.153 mg / 100 g, copper - 108 mg / 100 g, selenium - 0.3 mg / 100 g, zinc - 0.29 mg / 100 g of feed), soluble polysaccharide inulin (up to 10%), fructose, pectins, amino acids, all groups of vitamins A, B, C, E. Hay from the green mass of Jerusalem artichoke in biological value (80%) exceeds red clover (70%), vetch (66%) ), timothy grass (64%) and alfalfa (57%) (see the article by Reinhart et al. Jerusalem artichoke - a valuable culture // Rural m mechanic. - 1998. - No. 6. - S. 22-24).

Herbal flour - a source of vitamins, trace elements, amino acids, promotes the activation of reproductive function and stimulates the metabolism (metabolism) (see the book. "Feeding of agricultural animals. Handbook / Edited by A. P. Kalashnikov. - M. : Rosagropromizdat, 1988).

Flask - opalkristobolite rock - a mineral sorbent based on highly dispersed silica with particle sizes of 0.01-0.001 mm, binding mycotoxins (aflatoxin B ₁ , T-2 toxin, zearalenone, ochratoxin A), heavy metal salts, chemical toxins, radionuclides , gases and other metabolic products. Silica contains silicon, aluminum, iron, calcium, magnesium, carbon. Silicon provides tissue growth and hardening during the development and formation of the skeleton and is involved in bone mineralization even with a deficiency of calcium, phosphorus, chlorine, fluorine, sodium, sulfur and other chemical elements (Patent RU No. 2355187. IPC A23K 1/14, A23K 1 / 16, published May 20, 2009).

Although the well-known sorbent - flask is considered highly dispersed, however, its composition is very polymorphic - the particle size ranges from 0.01 to 0.001 mm. It was found that particles with a size of 0.01-0.09 mm have a very low sorption activity and therefore reduce the effectiveness of the sorbent. To increase the sorption activity of natural minerals, they are usually activated by the destruction of large particles with hydrochloric acid, followed by fractionation of particles (0.0006-0.0009 mm) and the removal of soluble salts, quartz (AS No. 952260, A61K 36/00, publ. 23.08.1982).

With this in mind, the activation of the mineral sorbent - flask is carried out by isolating from it the most active highly sorption fraction. For this flask is subjected to special treatment. To destroy carbonates, the mineral is first treated with 1 n, and then with 0.1 n hydrochloric acid. The resulting soluble salts and quartz are removed by washing with distilled water. To do this, the mineral is transferred with water to vegetation glasses (20 × 12 cm). Labels are applied to each glass: the first is at a height of 3 cm from the bottom, the second is 7 cm higher, the third is 7 cm higher than the second. Distilled water is added to the third mark, the flask is stirred up in it and made up for 12 hours to settle. When the solid part of the mineral settles and a clear liquid remains above it, the latter is removed by a siphon and distilled water is poured again to the top mark. When the suspension remains in suspension, proceed to the elutriation of particles with dimensions of 0.0006-0.0009 mm To do this, distilled water is poured into the glass to the top mark, the contents are mixed, after 24 hours the siphon is immersed to a depth of 7 cm and with its help the suspension is transferred to the receiver glass. Elutriation continues until the suspension is clarified. For each new portion of the suspension, 3-4 drops of concentrated hydrochloric acid are added to the receiver glasses for coagulation and sedimentation of the suspension. The fraction is washed with distilled water, the content and size of particles are determined in it by micrometrizing them on a microscope with a micrometer line, and then dried in a water bath and used as a sorption component in a composite sorption preparation.

The yield of the final product - activated (carrying the main useful function of the flask - sorption of toxicants and radionuclides) of the sorbent fraction in this case is 35-40% of the volume of the mineral.

Removal of inert components from the flask provides not only the active binding of radiocaesium and radiostrontium to the feed in the gastrointestinal tract, but also, unlike the untreated flask, is actively absorbed by the gastrointestinal mucosa and reaches the target cells (osteocytes, hepatocytes that have deposited isotopes) , myelocytes), binds isotopes incorporated by cells and removes them from the body, thereby performing active (in contrast to simple physico-chemical sorption of the isotope in the gastrointestinal tract tract-screw from the famous) metabolizing decorporation radionuclide.

The essence of the method of preparation of the feed additive is as follows.

At the first stage, powders are prepared from dead bees, drone brood and Jerusalem artichoke.

To obtain powder from the dead, freshly dead, dry bees without signs of decomposition of bees are collected. Raw materials are sieved through a sieve with large cells to separate it from small debris. After that, the scum is dried in the oven, oven at a temperature of + 40-45 ° C or over a central heating battery. The dry material is homogenized in a ball mill or electric grinder. The degree of dispersion is controlled microscopically, which should be in the range of 60-90 microns. The resulting powder is packaged in paper bags of 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 g and subjected to radiation treatment at a dose of 3000 Gy. In the process of radiation exposure, not only the death of contaminant microbes occurs, but also the radiochemical activation of chitin by radiolysis, during which disulfide bonds break with the formation of thiol groups (natural radioprotectors) with simultaneous deproteinization and demineralization, i.e. Chitin activation without traditional hydrolysis using technologically hazardous reagents - concentrated alkalis and acids. The powder obtained from the death of bees obtained in the above manner is used as one of the components of the feed additive.

To obtain the powder from the drone brood, the larvae at different stages of development are carefully removed with tweezers from the honeycomb cells, dried, subjected to homogenization and further processing according to the method described above for the manufacture of powder and bee death. The resulting powder is packaged in bags and subjected to radio sterilization, used as a component of a feed additive.

To obtain Jerusalem artichoke powder, ripened tubers (in September-October) are dug up, washed, dried, cut into small pieces, dried in an oven, oven at a temperature of 40-45 ° C or under a central heating battery. The dry material is homogenized in a ball mill or electric grinder. The dried powder is packaged in paper bags and stored at a temperature of 15-16 ° C for 24 months.

As an additional source of vitamins, trace elements, amino acids, an activator of reproductive function and a stimulator of metabolism (metabolism), herbal flour is used according to GOST 18691-83.

As a sorbent of toxicants and radioisotopes, activated by selective fractionation and selection of particles with a size of 0.0006-0.0009 mm of a natural mineral of opalcristobolic rock - flask is used.

Biologically active feed additive is made in the form of a powder as follows.

1. To obtain 100 kg of powder, 8 kg of ground bee subpestilence, 2.5 kg of bee brood, 67 kg of ground Jerusalem artichoke tubers, 2.5 kg of purified flask fraction and 13 kg of grass flour are taken in accordance with GOST 18691-83. The mixture of components is mixed until a homogeneous (homogeneous) mass is obtained and packaged in plastic bags of 25-30 kg and stored in a cool dark place at a temperature of 15-16 ° C for 24 months.

2. To obtain 100 kg of powder, 10 kg of bee subpestilence, 3 kg of bee brood, 69 kg of Jerusalem artichoke tubers, 3 kg of purified flask fraction and 15 kg of grass meal are taken in accordance with GOST 18691-83. The resulting mixture is stirred until a homogeneous mass and packaged in plastic bags.

As tests show, the use of the above ratios of components in the direction of reduction leads to a weakening of antibacterial, antitoxic, decorpore, antioxidant, anti-radiation, metabolism and immunostimulating effects, the body's resistance to factors of a physical, chemical and biological nature, and an increase in components is impractical due to overuse of components and only the use of components in this range ensures the best results (see below).

The developed biologically active feed additive can be recommended for use as a general strengthening, detoxifying, antioxidant, anti-infectious, immunomodulating, metabolism stimulating growth and development of young animals, a decorporator of radioisotopes and heavy metals, pesticides and neutralization of mycotoxins.

Example 1. The effectiveness of the use of feed additives for the growth, development and preservation of chickens

The stimulating effect of the feed additive on the growth energy and the safety of young chickens was carried out in a scientific and production experiment on broiler chickens at the age of 4 days, divided into 3 groups (2 experimental and 1 control). Chickens of the 1st group (20 goals) received the main diet with the addition of the declared feed additive in a dose of 0.4% to the main diet with two 10-day courses with an interval of 10 days between them. Chickens of the 2nd group (20 goals) received in the usual diet the well-known feed supplement “Vita-Forte M” in the same doses. Chickens of the 3rd group (20 goals) received a normal diet without feed additives (control). Evaluation of the effectiveness of the use of feed additives was carried out on growth-weight, clinical, hematological and immunobiological indicators. The results of the study are shown in the table.

It was found that the consumption of the claimed feed additive increases the safety of chickens by the end of the experiment (by 49 days) up to 98.8% compared to 91.7% and the average daily gain by 11.65% compared with the control, which indicates a positive effect declared feed additives on the state of natural resistance, the level of development of the immune system as the main component of the body's resistance to agents of infectious and non-infectious nature, the integral criterion of which is the safety of the population, and e increased productivity (weight gain).

Example 2. Evaluation of the effectiveness of the claimed feed additives for growth, development and preservation of young animals was carried out on 50-60-day-old piglets, divided into three groups. Piglets of the 1st group (10 goals) received a normal diet with the addition of the declared feed supplement 30 g per day for 30 days. Piglets of the 2nd group (10 goals) under the same conditions received the usual diet with the addition of the drug "Vita-Forte M". Piglets of the 3rd group received a normal diet without feed additives (control).

It was established that the gain in live weight of piglets of the 1st group for 30 days of feeding was 1.27 kg versus 0.54 in the control and 1.09 kg in the 2nd group, which is 1.86 times higher than the control and with the 2nd group - 1.16 times. The survival of piglets in the 1st and 2nd groups was 100%, in the 3rd - 70%. The number of cases in the 1st group was 1 head (10%), in the 2nd - 2 (20%) and in the 3rd - 4 (40%). Therefore, the inclusion in the main feed diet of piglets of the declared feed additive in an amount of 30 g per day for 30 days ensures 100% preservation of young animals, reduces the incidence of gastrointestinal and respiratory diseases by 4 times compared with the control and 2 times compared with those of the 2nd group (the well-known drug "Vita-Forte"), and the increase in live weight for this period was 1.27 kg versus 1.09 kg when feeding a known feed additive and 0.54 kg with a normal diet.

Example 3. Checking the antibacterial (anti-infectious) activity of the drug

To verify the activity of the drug, aqueous extracts of the claimed and known preparations were prepared with their subsequent dilution; 1: 2, 1: 4.1: 8, 1:16, 1:32, 1:64, 1: 128, 1: 256 and 1: 512. The prepared concentrations were added 1.0 ml in test tubes containing microbial cells of the virulent strain of the causative agent of Escherichiosis E. coli “KV-1” with a concentration of microbes of 5 × 10 ⁸ MK / ml, the mixture was thermostated for 24 hours, conducting control seeding samples from each tube with the appropriate dilution of extracts and the test microorganism after 1, 2, 3, 4, 8, 16, 20, 24 hours. Crops were examined for 10 days, recording the growth of colonies and comparing with control samples.

It was found that the death of test cultures when using the extract of the claimed drug occurred in 55 minutes when it was in contact with a dilution of the extract 1:32. In contrast to the claimed, in control samples using the extract of the well-known drug "Vita-Forte M", the death of the test cultures occurred in 240 minutes with a dilution of the drug 1:16, which is 2 times less than the claimed. The increase in the antibacterial activity of the proposed drug is due to the sum of the microbicidal effects of the apisogenic agents (dead bees, bee brood), phytogenic (Jerusalem artichoke, grass meal) and mineral (flask) nature.

Example 4. Checking the antimycotoxic activity of the claimed feed additives

To assess the antimycotoxic activity of the drug, mycotoxicosis was simulated by feeding animals with a diet containing T-2 toxin and zearalenone. Studies were performed on 30 white rats, divided into 3 groups of 10 animals each. Animals of the 1st group received a toxin at a dose of 0.13 mg / kg (1/10 LD ₅₀ ) + the declared feed additive with a T-2 diet, group 2 - zearalenone (0.14 mg / kg) + the declared feed additive , 3rd group - zearalenone + T-2 toxin (1: 1) on the background of feeding a normal diet without feed additives (control). Twenty days after the start of feeding, animals treated with mycotoxin-contaminated and containing the declared feed supplement were killed and samples were taken to indicate mycotoxins.

The results of the indication of T-2 toxin and zearalenone by the method of mass spectroscopy of extracts of organs and tissues of experimental and control rats showed that the content of T-2 toxin in those receiving the claimed feed additive was 5.3% (group 1), zearalenone - 4.7 % (2nd group) of the administered amount of mycotoxin. The content of mycotoxins in the organs of hunted animals on the background of feeding them with a normal diet exceeded that by 7.5 times, accounting for 35.2% of the administered amount of toxicants.

Example 5. Checking the antidote activity of the drug

For this purpose, experiments were carried out on 45 white mice, divided into 3 groups of 15 animals each. Animals of the 1st group were subjected to oral seeding for 5 days with cadmium chloride (CdCl ₂ ) at a dose of 1/5 LD ₅₀ (50.8 mg / kg), followed by feeding with a normal diet; 2nd — a similar seed followed by feeding with a diet containing the claimed feed supplement; animals of the 3rd group, poisoned by the toxicant in a similar way, received sorbent-flask with feed at the rate of 2% of the ration weight. The feed additive and sorbent were set within 30 days after the seed of cadmium chloride.

It was established that mortality from cadmium intoxication in the 1st group (toxicant control) was 30%, in the second - 0% and in the 3rd - 13.4%. Therefore, feeding the animals inoculated with heavy metal - cadmium chloride with the claimed feed additive had an antidote effect, providing 100% survival with a 5-fold intake of a dangerous toxicant (CdCl ₂ ) in a dose of 1/5 LD ₅₀

Example 6. Verification of the radioprotective effect of the drug

Experiments to verify the radioprotective activity of the inventive feed additives were performed on 50 white rats, divided into 5 groups of 10 animals each. Rats of the 1st group received a feed ration containing 30% of the dry feed of the claimed feed additive (prophylactic use) within 30 days prior to irradiation, and the 2nd group — under the same conditions and doses, received the test feed supplement after irradiation (treatment option) ), The 3rd group — in the same doses and conditions — the well-known Vita-Forte feed additive before irradiation, the 4th group — in the identical conditions and doses, the well-known feed additive after irradiation. The irradiated animals of the 5th group were kept on a normal diet and they served as a radiation control.

Animals of all groups were exposed to external gamma radiation at a dose of 9.0 Gy (LD _100/30 ), causing severe acute radiation sickness (ARS) with fatal outcome.

It was found that the survival rate of animals in the 1st group was 80%, in the 2nd - 70%, in the 3rd - 30%, in the 4th - 20% with the radiation death of all animals in the 5th group.

Example 7. Verification of the decorpore activity of the inventive feed additive in relation to the radioisotope of cesium - 137.

To evaluate the decorpore (radionuclide-binding and isotope-removing) activity of the drug, experiments were performed on 30 white rats, divided into 5 groups of 6 animals each. Animals of all groups were injected three times intragastrically with an aqueous solution of cesium-137 with a total activity of 1000 Bq / kg. After completing the seeding for 20 days, the animals of the 1st group received a diet with the addition (at the rate of 3% of dry feed) of the well-known feed additive “Vita-Forte M”, the 2nd group — the claimed feed additive, the 3rd — bentonite, 4th — activated flask. Hunted animals of the 5th group received a normal diet without additives.

The results of radiometry of samples of organs and tissues of slaughtered 20 days after the start of feeding the animals with feed containing test additives and sorbents showed that the residual radioactivity in the critical organ - muscle tissue of animals of the 1st group (Vita-Forte M) was 397.1 Bq / kg, 2nd (declared additive) - 209.7 Bq / kg, 3rd - 307.5 Bq / kg, 4th - 271.7 Bq / kg and 5th (control) - 797, 9 Bq / kg.

Thus, in terms of radiocaesium-binding and excretory (deprotecting) activity, the inventive preparation exceeds the known 1.89-fold, sorbent (bentonite) regulated by cesium contamination - 1.47 times. The use of the purified (activated) flask fraction significantly increases the sorption activity of the mineral, exceeding the known sorbent - bentonite by 1.32 times, and the inclusion of the purified (activated) version of the flask in the claimed feed additive, due to the synergistic mutually reinforcing effect of its components - pectins and Jerusalem artichoke inulin, significantly increase the cesium-binding activity of the claimed drug.

Example 8. Checking the decorpore activity of the inventive feed additives in relation to the radioisotope strontium-90

To assess the decorpore activity of the claimed drug in relation to another long-lived isotope - strontium-90 experiments were conducted on 30 white rats, divided into 5 groups of 6 animals each. Animals of all groups were injected three times intragastrically with an aqueous solution of strontium-90 with a total activity of 1000 Bq / kg.

After seeding was completed for 20 days, the animals of the 1st group received a diet with the addition (at the rate of 3% of dry food) of the well-known feed additive “Vita-Forte M”, the 2nd group — the claimed feed additive, the 3rd — regulated when strontium incorporation ferrocin, 4th — activated flask. Group 5 animals poisoned with strontium-90 received a normal diet without feed additives and sorbents.

The results of radiometry of bone samples (a critical organ for osteotropic radiostrontium) inoculated with strontium-90 and treated with feed additives and sorbents and killed 20 days after the start of feeding the animals showed that the residual radioactivity in the critical organ (bone tissue) of animals of the 1st group was 53, 5%, 2nd - 18.1%, 3rd - 16.7%, 4th - 17.3% and 5th - 91.5% of the entered amount.

Thus, the claimed preparation is 2.95 times superior in terms of strontium-decorporizing activity to the known feed additive and in this indicator it is almost inferior to the sorbent-ferrocin regulated for strontium incorporation of the body. It should be noted that ferrocin is a synthetic deficient foreign drug that is toxic due to the presence of N≡C in the cyan group, i.e. a cyanogenic preparation which, when feeding animals with cyanogenic feeds (sorghum, vetch, castor oil, lupine), usually causes poisoning of animals with a high fatal outcome. In addition, the specified sorbent does not have any beneficial properties for the body.

Therefore, the inclusion of the activated fraction of the natural mineral - flask in the composition of the feed additive allows not only to effectively cleanse the body of the most dangerous isotopes: - cesium-137 and strontium-90, but also to stimulate the metabolism in the body, increase the productivity and preservation of animals, and in the event of a change in the environmental situation, take measures to cleanse the body of heavy metals, radio and mycotoxins, pathogenic microorganisms, radionuclides of various classes (myotropic and osteotropic radioisotopes PAE), which distinguishes it from analogues and the prototype.

The proposed biologically active feed additive for birds and farm animals can increase the biological activity of the drug while reducing its cost and simplify the technology for its production.

The inclusion of activated flask, chitin- and pectin-containing raw materials, such as Jerusalem artichoke and bee killing, in the composition of the feed additive allows expanding the possibility of using the drug in various forms of animal pathology caused by agents of a chemical, physical and biological nature.

The economic effect of introducing the drug into animal husbandry and veterinary medicine consists of increasing feeding efficiency, increasing weight gain, preserving the number of young animals, preventing diseases of an infectious and non-infectious nature, proceeding by type of gastrointestinal upset, damage to the respiratory system, as well as in changing environmental conditions in certain areas of the country.

Tests of the proposed feed additive were conducted in livestock farms of the Tukaevsky district of the Republic of Tatarstan, which showed an increase in growth of 20.9% and the safety of young animals by 31.7%. It is planned to introduce the proposed additive in livestock and poultry farms of the Republic of Tatarstan in 2016.

Claims (3)

1. A biologically active feed additive for farm animals and poultry, containing bee killing, drone brood at different stages of development, grass flour, characterized in that it additionally contains an activated sorbent fraction - flask and Jerusalem artichoke tubers powder in the following ratio of components, wt.%: Bee death 8-10 Drone brood in different stages of development 2.5-3 Herbal flour 13-15 Activated flask 2.5-3 Jerusalem artichoke tubers powder rest 2. The biologically active feed additive according to claim 1, wherein the activated flask is a flask treated with hydrochloric acid, followed by fractionation and selection of particles of 0.0006-0.0009 mm.