RU2511061C1 - Method for dermal flap survival growth in reduced circulation with resveratrol - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: dermal flap is simulated in laboratory animals on the second day of experiment. Resveratrol is administered ingragastrically in a daily dose of 2.0 mg/kg from the first experimental days every 46 hours.

EFFECT: method is non-toxic, has minimum contraindications, provides the dermal flap survival growth in the reduced circulation, by activating a pre-conditioning process.

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Description

The invention relates to medicine, in particular to experimental surgery and experimental pharmacology, and can be used to correct ischemia.

A known method of increasing the survival of a skin flap on a leg by distant ischemic preconditioning RU 2429547 C1. Its disadvantage is that the method is not without the potential danger of ischemic episodes for pathologically altered tissues and is applicable mainly to a healthy body.

Closest to the claimed solution is the "Method of pharmacological correction of survival rates of ischemic tissues in the conditions of reduced blood circulation" (RU 2438190 C1). Correction of ischemia is carried out by recombinant erythropoietin. The advantage of this method is that the activation of the preconditioning process is carried out by a pharmacological preparation. That is, there is no episode of ischemia, which can have a damaging effect on the tissue. However, the disadvantage of this method is that erythropoietin is chosen as the initiator of the preconditioning process. Numerous researchers tend to consider him the humoral agent of preconditioning. However, this is a hormonal protein preparation and there are a number of contraindications for its use, namely arterial hypertension, cancer. That is why it is important to study drugs that can activate various stages of the preconditioning process.

The technical result of the invention is the development of an effective method for increasing the survival of a skin flap in conditions of reduced blood circulation, including the use of a pharmacological preparation of plant origin, capable of initiating a preconditioning process, non-toxic, with a minimum number of contraindications.

The technical result is achieved by the fact that when simulating a skin flap under reduced blood circulation on the second day of the experiment, from the first day every 46 hours of the experiment, resveratrol is administered intragastrically to the laboratory animal in a daily dose of 2.0 mg / kg.

The main advantage of the proposed method is the absence of side effects (except for individual intolerance) of the pharmacological agent, since it belongs to the class of phytoalexins. In nature, it exists as a glycoside and two cis and trans stereoisomers of aglycon. Both stereo forms (cis and tras) are found in the root of the highlander (Polygonum cuspidatum), and the transformation was found in the skin of grapevines (Vitis vinifera L.) and in peanuts. A higher concentration is found in red wine. To date, it is known that it is found in more than 70 plants. These are different types of currants, rhubarb, pine peanuts, etc. The studied mechanisms of cardioprotection by resveratrol indicate its ability to initiate the preconditioning process and allow us to study the possibility of its universal use in surgery as a drug for pharmacological preconditioning.

The method is carried out as follows.

The experiments were carried out on white Wistar rats weighing 220-250 g. Modeling of the skin flap was carried out under anesthesia (chloral hydrate 300 mg / kg ip) on the second day of the experiment. Having departed 1 cm from the xiphoid process along the white line of the abdomen, having previously sized the size of the stencil, cut out a skin flap: 1 cm - base, 4 cm - length (keeping the feeding vessel), which is isolated in a plastic bag, the edges of the skin are sutured with a continuous suture. The survival of a skin flap is evaluated on the sixth day of the experiment, by measuring the area of the surviving flap tissue in the group of animals with modeling of the skin flap and in the group with modeling of the skin flap and resveratrol.

Resveratrol is administered intragastrically at a daily dose of 2.0 mg / kg from the first day every 46 hours of the experiment.

When statistical data processing is calculated, the average value, the standard deviation. Differences are considered significant at p <0.05.

EXAMPLE OF SPECIFIC IMPLEMENTATION.

The experiments were carried out on white rats male Wistar strain weighing 220-250 g. The experimental animals were divided into the following groups:

1. Control (n = 10).

2. Using resveratrol (n = 10).

Animals of both groups had a skin flap modeled on the second day of the experiment. Under anesthesia (chloral hydrate 300 mg / kg intraperitoneally), departing 1 cm from the xiphoid process along the white line of the abdomen, having previously sized the stencil, a skin flap was cut out: 1 cm - base, 4 cm - length (keeping the feeding vessel), which was isolated in a plastic bag, the edges of the skin were sutured with a continuous suture. The animals of the second group were intragastrically administered resveratrol in a daily dose of 2.0 mg / kg from the first day every 46 hours of the experiment.

Assessment of the survival of the skin flap was performed on the sixth day of the experiment, measuring the area of healthy tissue.

The results of the evaluation of the survival of the skin flap are presented in table 1.

Administration of resveratrol to experimental animals contributed to a significant (p <0.05) increase in the area of surviving flap tissue.

Table 1 The survival rate of the skin flap in the conditions of reduced blood circulation on the 6th day of the experiment (5th day after modeling) (M ± m) Group (n = 10) Healthy flap tissue area in cm % Modeling of the skin flap (control) 1.6 ± 0.02 40 Modeling a skin flap + resveratrol 2.9 ± 0.18 * 73 Note. * - p≤0.05 when compared with the control group; resveratrol intragastrically, in a daily dose of 2.0 mg / kg from the first day every 46 hours of the experiment

Thus, the obtained results allow one to ascertain an increase in the survival of a skin flap under conditions of reduced blood circulation, modeled on the second day of the experiment, with intragastric administration of resveratrol in a daily dose of 2.0 mg / kg from the first day every 46 hours of the experiment.

Claims (1)

A method for increasing the survival of a skin flap under conditions of reduced blood circulation, including modeling a skin flap in an experimental animal and administering a pharmacological agent, characterized in that the simulation is carried out on the second day of the experiment, and resveratrol is administered as a pharmacological agent, which is administered to the animal intragastrically at a daily dose of 2, 0 mg / kg from the first day every 46 hours of the experiment.