RU2429547C1 - Method for improving survival rate of ischemic tissues (isolated skin pedicle flap) in reduced blood circulation - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: for correction of tissue ischemia. For this purpose, in a laboratory animal, skin flap dissection is preceded by distant pre-conditioning by 10-minute femoral artery cross-clamping. It is followed with reperfusion for 30 minutes.

EFFECT: method allows increasing a healthy tissue area in the conditions of the reduced blood circulation with no drugs used.

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Description

The invention relates to medicine and can be used to correct ischemia.

Closest to the claimed solution is to study the effect of the macrolide preparation of azithromycin on the survival of a skin flap on a leg described by E. Chernomortseva and other patent for the invention RU 2283116 C2.

However, this solution is possible only with the use of the drug, which entails a number of side effects.

The objective of the invention is to develop a method for improving the survival rate of ischemic tissues (isolated skin flap on the leg) in conditions of reduced blood circulation, excluding the use of drugs.

The task is achieved by the fact that in order to improve the survival rate of ischemic tissues (isolated skin flap on the leg) in the conditions of reduced blood circulation, remote preconditioning is performed.

The method is carried out as follows.

The experiments were carried out on white rats of Wistar male males weighing 200-220 g. Modeling of the skin flap was carried out under anesthesia (chloral hydrate 50 mg / kg ip). Retreating 1 cm from the xiphoid process, a skin flap 1 cm is cut out - the base, 4 cm - length, is isolated in a plastic bag, the edges of the skin are sutured with a continuous suture. Assessment of the survival of the skin flap is carried out on the fifth day, by measuring the area of healthy tissue. The survival of a skin flap is assessed in the group with a simulated skin flap and in the group with distant preconditioning preceding it. Distant preconditioning is carried out with a 10-minute clamping of the femoral artery followed by a 30-minute reperfusion.

When statistical data processing is calculated, the average value, the standard deviation. Differences are considered significant at p <0.05.

EXAMPLE OF SPECIFIC IMPLEMENTATION.

The experiments were carried out on white rats male Wistar line weighing 200-220 g. Experimental animals were divided into the following groups:

1. control (n = 10);

2. using distant preconditioning (n = 10).

All animals had a skin flap modeled. Under anesthesia (chloral hydrate 50 mg / kg ip), slipping 1 cm from the xiphoid process, a skin flap 1 cm - base, 4 cm - length was cut out, isolated in a plastic bag, the edges of the skin were sutured with a continuous suture. In the second group of animals, distant preconditioning was performed with 10 minutes of compression of the femoral artery followed by 30 minutes of reperfusion. Assessment of the survival of the skin flap was performed on the fifth day, measuring the area of healthy tissue.

The results of the evaluation of the survival of the skin flap are presented in the table.

The survival rates of an isolated skin leg flap on the 5th day after modeling (M ± m). Group (n = 10) Healthy tissue area cm % Isolated skin flap (control) 1.59 ± 0.03 39.75 Isolated skin flap + distant preconditioning 2.37 ± 0.15 * 59.25 Note: * - p≤0.05 when compared with the control group.

In the control group, the area of healthy tissue on the fifth day was 1.59 ± 0.03 cm, i.e. 39.75% of the initial area of the skin flap. In the group of animals that underwent distant preconditioning before cutting an isolated skin flap on a leg, the area of healthy tissue increased to 2.37 ± 0.15 cm, i.e. 59.25% (table).

Thus, the obtained results allow us to state an improvement in the survival rates of ischemic tissues in the conditions of reduced blood circulation under the influence of distant preconditioning by 1.49 times compared with the control group.

Claims (1)

A method for improving the survival rate of ischemic tissues under conditions of reduced blood circulation, characterized in that the laboratory animal undergoes distant preconditioning with a 10-minute clamping of the femoral artery before cutting the skin flap followed by a 30-minute reperfusion.