RU2507598C1 - Method for pharmacological stimulation of pedicle skin flap survival with nicorandyl - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: pedicle skin flap survival is corrected in laboratory animals is corrected by modelling a flap on the second experimental day. On the first and fourth day of the experiment, nicorandyl is introduced intraperitoneally in a daily dose of 1.3 mg/kg in two stages.

EFFECT: higher skin flap survival in the reduced blood circulation environment ensured by opening the ATP-dependent potassium channels and providing the nitrate-like effect of nicorandyl in the specific experimental environment.

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Description

The invention relates to medicine, in particular to experimental pharmacology, and can be used to correct ischemia.

Closest to the claimed solution is a method of pharmacological correction of survival rates of ischemic tissues in the conditions of reduced blood circulation RU 2429547 C1, 12/27/11.

However, this decision does not assess the effect of nicorandil on the survival of the skin flap on the feeding leg.

The advantage of this method is that the increased survival of the skin flap on the feeding leg is achieved by activation of the preconditioning effector link - ATP-dependent potassium channels with a pharmacological preparation. In addition, it has been proven that nicorandil is free from unwanted side effects at the dose used.

The technical result of the invention is the development of a method for pharmacological stimulation of the survival of a skin flap on a feeding leg, including the use of nicorandil.

The technical result is achieved by the fact that in order to pharmacologically stimulate the survival of a skin flap on the feeding leg, modeled on the second day of the experiment, Nicorandil is administered intragastrically to the experimental animal in a daily dose of 1.3 mg / kg on the first and fourth days of the experiment.

The method is as follows.

The experiments were carried out on white Wistar rats weighing 220-250 g. Modeling of the skin flap was carried out under anesthesia (chloral hydrate 300 mg / kg ip) on the second day of the experiment. Having departed 1 cm from the xiphoid process along the white line of the abdomen, having previously sized the size of the stencil, cut out a skin flap: 1 cm - base, 4 cm - length (keeping the feeding vessel), isolated in a plastic bag, the edges of the skin are sutured with a continuous suture. The assessment of the survival of the skin flap is carried out on the sixth day of the experiment by measuring the area of the surviving flap tissue in the group of animals with modeling of the skin flap and in the group with modeling of the skin flap and the introduction of nicorandil.

Nicorandil (Coronel, Russia) is administered intragastrically at a daily dose of 1.3 mg / kg on the first and fourth days of the experiment.

When statistical data processing is calculated, the average value, the standard deviation. Differences are considered significant at p <0.05.

EXAMPLE OF SPECIFIC PERFORMANCE

The experiments were carried out on white rats male Wistar strain weighing 220-250 g. The experimental animals were divided into the following groups:

1) control (n = 10);

2) using nicorandil (n = 10).

Animals of both groups had a skin flap modeled on the second day of the experiment. Under anesthesia (chloral hydrate 300 mg / kg intraperitoneally), departing 1 cm from the xiphoid process along the white line of the abdomen, having previously sized the stencil, cut out a skin flap: 1 cm - base, 4 cm - length (keeping the feeding vessel), isolated a plastic bag, the edges of the skin were sutured with a continuous suture. The animals of the second group were intragastrically injected with nicorandil in a daily dose of 1.3 mg / kg on the first and fourth days of the experiment.

Assessment of the survival of the skin flap was performed on the sixth day of the experiment, measuring the area of healthy tissue.

The results of the assessment of the survival of the skin flap on the feeding leg are presented in table 1.

Table 1 The survival rate of the skin flap on the feeding leg on the 5th day after modeling (6th day of the experiment) (M ± m) Group (n = 10) Healthy flap tissue area in cm % Modeling of the skin flap (control) 1.6 ± 0.02 40 Modeling a skin flap + nicorandil 3.0 ± 0.04 * 75 Note. * - p≤0.05 when compared with the control group; Nicorandil intragastrically in a daily dose of 1.3 mg / kg on the first and fourth days of the experiment.

Administration of nicorandil to experimental animals contributed to a significant (p <0.05) increase in the area of surviving flap tissue.

Thus, the obtained results allow us to state pharmacological stimulation of the survival of a skin flap on a feeding leg, modeled on the second day of the experiment, by intragastric administration of Nicorandil in a daily dose of 1.3 mg / kg on the first and fourth days of the experiment.

Claims (1)

A method for pharmacologically stimulating the survival of a skin flap on a feeding leg, including modeling a skin flap in an experimental animal and administering a pharmacological agent, characterized in that the simulation is carried out on the second day of the experiment, and nicorandil is used as a pharmacological agent, which is administered to the animal intragastrically at a daily dose of 1, 3 mg / kg on the first and fourth days of the experiment.