RU2489906C1 - Biologically active denaturated collagen based food additive - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to production of denaturated collagenic protein based biologically active additives and may be used as a functional biologically active food additive for strengthening connective tissue. The biologically active food additive includes the following components: denaturated collagenic proteins of skin or cartilage or ligaments or sinews or their mixture, vitamins C, B6, PP, calcium carbonate, ferric sulphate, cuprum citrate, magnesium citrate, sodium thiosulphate, laminaria blade, girasol leaf or root or their mixture and a filler. The biologically active substance composition depends on age and physical peculiarities of the human.

EFFECT: invention allows to produce functional active food bioadditives eliminating connective tissue deficit with various categories people and stimulating connective tissue recovery after diseases, traumas and hard physical activities.

6 cl, 4 tbl

Description

The invention relates to the production of biologically active additives based on denatured collagen proteins in combination with vitamins, macronutrients, microelements, medicinal plants and can be used as a functional active dietary supplement for athletes and different population groups as a means of stimulating the formation of new collagen fibers for strengthening connective tissue.

Known biologically active food supplement (BAA) “Collagen ULTRA-SI”, manufactured in LLC “Alina Pharma” (State Registration Certificate No. 77.99.23.3.U.3135.5.07) in the form of a powder containing the following components: collagen hydrolyzate, maltodextrin, xanthan gum, vitamin C, flavor, sweetener Sladin, dye E102, E110, E111 or E124, citric acid (E330). This supplement is a complex of collagen hydrolyzate, vitamin C, starch, dyes and stabilizers. The disadvantages of this dietary supplement are that of the necessary components involved in the formation of collagen fibrils and connective tissue fibers, there are only two: collagen in the form of a hydrolyzate and vitamin C. This composition weakly affects the elimination of connective tissue deficiency and does not stimulate the formation of new connective tissue.

Known biologically active additive "Gelenk Narung" made in "ProVista AG" ("ProVista AG") in Germany (State Registration Certificate No. 77.99.23.3.U.3191.4.09) in the form of a powder containing the following components: collagen hydrolyzate, gelatin, fruit powder, citric acid, dextrose, aspartame K, acesulfame K, maltodextrin, vegetable oil and flavorings. This additive is a complex of collagen hydrolyzate, gelatin, potassium salts, flavorings and stabilizers. Collagen proteins are presented in the form of a hydrolyzate and denatured collagen - gelatin. The disadvantages of this dietary supplement include the fact that the composition does not contain substances that activate the formation of collagen fibrils and connective tissue fibers. Such a set of substances does not provide a quick restoration of connective tissue.

Known biologically active food supplement “Super Shark Cartilage”, manufactured in LLC “APT Modern Scientific Technologies” (State Registration Certificate No. 77.99.13.3.U.4569.6.10) in the form of tablets and capsules, containing the following components: food collagen hydrolyzate "Collamine-80", the raw material for the dietary supplement "Shark cartilage-raw materials", the fat component "Delios S", maltodextrin "Glyusideks 19", crystalline fructose, magnesium stearate. This supplement is a complex of collagen hydrolyzate, shark cartilage extract, fat component of vitamins, excipients: crystalline fructose and magnesium stearate. The disadvantages of this dietary supplement are that of the necessary components involved in the formation of collagen fibers of connective tissues, there are only three: collagen in the form of a hydrolyzate, glycosaminoglycans of shark cartilage and magnesium. This composition can disrupt the correct structure of fibrils at the initial stages of fibrillogenesis, since glycosaminoglycans in combination with salts stimulate the formation of collagen fibers only at the last stage of fibrillogenesis. Nevertheless, under physiological conditions and at low glycosaminoglycan concentrations, collagen fibrils are formed, and glycosaminoglycan concentrations are an order of magnitude lower than collagen concentrations (Stuart K., Panitch A. Influence of chondroitin sulfate on collagen gel structure and mechanical properties at physiologically relevant levels. Biopolymers. 2008 . V.89. P.841-851). The selected ratios of the concentrations of collagen and glycosaminoglycans correspond to similar ratios of their concentrations in the fibrils of connective tissues (Nimni ME, Harkness RD Molecular structures and functions of collagen. In “Collagen.” 1988. Boca Raton. CRC Press. V.1. P.1-77 ) The disadvantages of the Super Shark Cartilage dietary supplement are a significant excess of the amount of shark cartilage (about two to three times) compared to the collagen content, which can lead to a chaotic structure of fibrils and fibers and ultimately to a change in the correct structure of cartilage, joints and ligaments.

The disadvantages of these dietary supplements include the fact that they do not provide stimulation of the formation of collagen fibers of connective tissue and do not eliminate connective tissue insufficiency.

The closest in technical essence is the biologically active food supplement “Collagen-S” made in LLC “Modern Scientific Technologies” ((The catalog of sports nutrition published by LLC “APT Modern Scientific Technologies” of the IRONMAN series in 2010, p. 41)) in the form of a powder containing the following components: collagen hydrolyzate, gelatin, ascorbic acid (vitamin C). This additive is a complex of collagen hydrolyzate, gelatin and vitamin C. The disadvantages of this dietary supplement are that it contains few substances that affect the formation of collagen fibers of connective tissue. Such a set of substances does not provide stimulation of the formation of collagen fibers of connective tissue and does not eliminate connective tissue insufficiency.

The objective of the invention is the creation of a biologically active food supplement, suitable for stimulating the formation of collagen fibers during restoration of skin tissue and cartilage, and ligaments, and tendons and suitable for strengthening these connective tissues.

The problem is solved by a functional-active biological supplement based on denatured collagen, including collagen proteins in denatured form and vitamin C, which according to the invention additionally contains vitamin B6, vitamin PP, calcium carbonate, iron sulfate, copper citrate, magnesium citrate, thallus kelp, Jerusalem artichoke leaf or root, or their mixture and filler in the following ratio of components, mg:

Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 1800-3800 Vitamin C 10-200 Vitamin B6 0.1-1.0 Vitamin PP 2.0-8.0 Iron sulfate 1,5-10,0 Copper citrate 0.2-1.0 Calcium carbonate 150-400 Magnesium citrate 75-250 Laminaria thallus 200-400 Jerusalem artichoke leaf or root, or a mixture thereof 100-250 Filler Up to 6000

In the case when the supplement is intended primarily for the nutrition of children, it contains the following ratio of components (Composition No. 1), mg:

Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 1800-2200 Vitamin C 10-30 Vitamin B6 0.1-0.5 Vitamin PP 2.0-4.0 Iron sulfate 1.5-2.5 Copper citrate 0.2-0.3 Calcium carbonate 150-250 Magnesium citrate 75-120 Laminaria thallus 200-250 Jerusalem artichoke leaf or root, or a mixture thereof 100-150 Filler: Up to 6000

The proposed additive may further include sodium thiosulfate in any physiological amount.

In the case when the supplement is intended primarily for nutrition of adult weakened people, it contains the following ratio of components (Composition No. 2), mg:

Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 2600-3000 Vitamin C 80-120 Vitamin B6 0.4-0.6 Vitamin PP 3.0-5.0 Iron sulfate 4.0-6.0 Copper citrate 0.4-0.8 Calcium carbonate 250-350 Magnesium citrate 150-200 Sodium thiosulfate 200-250 Laminaria thallus 220-300 Jerusalem artichoke leaf or root, or a mixture thereof 125-175 Filler: Up to 6000

In the case when the supplement is intended primarily for the nutrition of women, it contains the following ratio of components (Composition No. 3), mg:

Denatured collagen skin proteins, or cartilage, or ligaments, or tendons, or a mixture thereof 2800-3200 Vitamin C 120-150 Vitamin B6 0.6-0.8 Vitamin PP 4.0-6.0 Iron sulfate 5.0-7.0 Copper citrate 0.5-0.9 Calcium carbonate 300-350 Magnesium citrate 180-240 Sodium thiosulfate 240-280 Laminaria thallus 250-350 Jerusalem artichoke leaf or root, or a mixture thereof 140-200 Filler: Up to 6000

In the case when the supplement is intended primarily for nutrition of men subject to great physical exertion, it contains the following ratio of components (Composition No. 4), mg:

Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 3400-3800 Vitamin C 150-200 Vitamin B6 0.8-1.0 Vitamin PP 6.0-8.0 Iron sulfate 7.0-10.0 Copper citrate 0.6-1.0 Calcium carbonate 350-400 Magnesium citrate 200-250 Sodium thiosulfate 250-300 Laminaria thallus 300-400 Jerusalem artichoke leaf or root, or a mixture thereof 150-250 Filler: Up to 6000

As a filler, the additive contains, for example, buckwheat flour, or oat flour, or rice flour.

The active additive is prepared in the form of powder, or tablets, or capsules, or jelly, or jelly, or “bars”.

The invention consists in the following.

The mechanical strength of connective tissues: skin, tendons, ligaments, cartilage and other tissues is determined by the structure of collagen fibrils and their fibers. With injuries, injuries, bruises and burns, the integrity of these tissues is violated. Diseases such as allergies, dermatitis, neurodermatitis, psoriasis, eczema, diathesis, shingles and others are associated with a lack of collagen of various types, as well as certain vitamins and salts [Alekseev A.A. Integrative (systemic, family) connective tissue medicine. (General practitioner). M .: LENAND, 2005, T. 4. S.1543-2130]. To restore the structure of the skin and other organs prone to diseases and injuries, it is proposed to select all the necessary components and stimulate the formation of new tissue by cells. The main element of connective tissue is collagen proteins specific to a particular tissue. The selection of components essential for the nutrition and restoration of the connective tissue of the skin, tendons and ligaments (mainly collagen type I), as well as cartilage (mainly collagen type II) is carried out taking into account the peculiarities of the biosynthesis of collagen proteins in these tissues. As the main source of amino acids for the synthesis of new collagen, fibrils and fibers, skin, cartilage, ligaments, tendons of farm animals are used. Vitamins, macroelements, microelements are regulators and catalysts of biochemical processes, are part of enzymes. The human body vitamins are not synthesized or synthesized in insufficient quantities. Therefore, vitamins should be supplied regularly with food.

In the human body, the process of starting the formation of polypeptide α-chains from amino acids, then the binding of α-chains to the triple helix of collagen and then the modification of collagen molecules with the formation of aldehyde groups for the participation of these groups in the creation of strong covalent bonds is activated by divalent iron and copper, as well as vitamins C , B6, PP. To activate the binding of collagen molecules to fibrils, and fibrils into fibers, calcium and magnesium are needed. The strength of collagen fibrils and fibers in tissues depends on the content of amino acids, which include sulfur. Given the structure of collagen fibrils and fibers, as well as the main elements that affect the process of collagen fibrillation and the mechanical strength of collagen fibers in connective tissues, the following set of components that are necessary for nutrition and restoration of connective tissues are proposed: collagen proteins, vitamin C, vitamin B6, vitamin PP, iron, copper, calcium, magnesium, sulfur.

It is known that, depending on the age and physical condition of a person, the content of collagen proteins in tissues changes; therefore, different concentrations of necessary components are required for different age groups of a person. The most sensitive to the lack of collagen proteins, which are the building material for the neoplasm of all human connective tissues, are certain categories of people: children, pregnant women, weakened people and old people. An increased content of protein food is necessary for adolescents and adults exposed to great physical exertion (Kuznetsova VN Health from nature: dietary supplements, vitamins, enzymes. Home encyclopedia. St. Petersburg: Nevsky prospect; Vector, 2007, 204 p.). Therefore, various functionally active biological supplements from collagen proteins in combination with vitamins and salts are offered. To regulate and stimulate the restoration of connective tissues, the composition and concentration of components vary.

Composition No. 1 - for children.

Composition No. 2 - for adults, mainly weakened and sick people, especially in the pre- and postoperative period, for people with genetically weak connective tissue and with collagenopathologies, for the elderly and old people.

Composition No. 3 - for women, mainly for female adolescents, for pregnant women and nursing mothers, for women athletes.

Composition No. 4 - for men, mainly for male adolescents, for men of physical labor and for athletes who need restoration of connective tissue after intense physical exertion.

Skin or cartilage, or ligaments, or tendons of farm animals, mainly pigs, are used as a source of collagen proteins. For faster assimilation by the human body, tissues are converted into a homogeneous suspension, and collagen proteins into a denatured form. Under the influence of enzymes of the gastrointestinal tract, protein denatured alcohol is hydrolyzed to amino acids.

Calcium, magnesium and copper are included as organic salts. It is known that organic compounds of salts, such as magnesium citrate and calcium citrate, are well absorbed in the gastrointestinal tract. Iron sulfate - a salt containing both iron and sulfur, is part of a functionally active dietary supplement. Sodium thiosulfate is an additional source of sulfur, since in case of skin diseases the human body needs a large amount of sulfur. Inorganic salts are absorbed worse compared to organic salts. Therefore, an additional natural source of salts are medicinal herbs and plant roots. The plants that contain a unique vitamin-mineral complex are introduced into the composition of a functionally active bioadditive.

Laminaria is especially useful for the human body, because it contains many salts, including salts of calcium, magnesium, silicon, iron, copper. Laminaria also contains vitamin C, B vitamins and pectin. It is known that with the help of kelp allergic skin diseases and inflammatory joint diseases are treated.

Jerusalem artichoke contains all the necessary vitamins and salts involved in the formation of collagen fibrils and stimulating the formation of connective tissue. Jerusalem artichoke contains vitamins B6, C, PP, as well as calcium, magnesium, iron, copper and silicon. Silicon compounds are also necessary for the normal functioning of connective tissue. Silicon improves the absorption of iron and calcium by the body. Jerusalem artichoke is rich in pectin, proteins and those amino acids that are part of the collagen structure: arginine, valine, lysine, leucine, etc.

The invention is as follows.

The technology for producing denatured collagen is as follows:

denatured collagen is obtained from the skin or cartilage, or ligaments, or tendons of farm animals, which are taken immediately after their slaughter, and then pre-degreased tissue with a solution of 0.5 M Ca (OH) ₂ , washed with plenty of water to remove alkali and treated tissue 70 % solution of ethyl alcohol for 1-2 hours to remove pathogenic bacteria and microorganisms. Then, tissue homogenization is carried out at a KM-100 colloid mill (Russia) and thermal denaturation of collagen proteins is performed at a temperature of 90-100 ° C for 1-3 hours. The homogenate is dried and the remaining components are added to the resulting powder in a certain ratio.

As a filler, they mainly take buckwheat flour, or oatmeal, or rice flour. In addition, any pharmacologically acceptable substance, for example, maltose, starch, maltodextrin, gums, talc, citric acid and the like, can be used as filler.

Receive the following functionally active biological additives.

Table 1 Composition 1 No. Name of components The content of the component, mg one 2 3 Denatured collagen proteins of the skin, or cartilage, or ligaments, or tendons, or a mixture thereof one 2100 1800 2200 2 Vitamin C 18.8 10.0 30,0 3 Vitamin B6 0.3 0.1 0.5 four Vitamin PP 3.0 2.0 4.0 5 Iron sulfate 1.8 1,5 2,5 6 Copper citrate 0.25 0.2 0.3 7 Calcium carbonate 210 150 250 8 Magnesium citrate 98.7 75 120 9 Laminaria thallus 230 200 250 Jerusalem artichoke leaf, or root, or a mixture thereof 10 128 one hundred 150 eleven Filler - buckwheat flour Up to 6000 mg

table 2 Composition 2 No. Name of components The content of the component, mg one 2 3 Denatured collagen proteins of the skin, or cartilage, or ligaments, or tendons, or a mixture thereof one 2800 2600 3000 2 Vitamin C 194.8 80 120 3 Vitamin B6 0.5 0.4 0.6 four Vitamin PP 4.0 3.0 5,0 5 Iron sulfate 4.8 4.0 6.0 6 Copper citrate 0.6 0.4 0.8 7 Calcium carbonate 288 250 350 8 Magnesium citrate 184 150 200 9 Sodium thiosulfate 218 200 250 10 Laminaria thallus 270 220 300 eleven Jerusalem artichoke leaf, or root, or a mixture thereof 150 125 175 12 Filler - buckwheat flour Up to 6000 mg

Table 3 Composition 3 No. Name of components The content of the component, mg one 2 3 one Denatured collagen proteins of the skin, or cartilage, or ligaments, or tendons, or a mixture thereof 3000 2800 3200 2 Vitamin C 138.6 120 150 3 Vitamin B6 0.7 0.6 0.8 four Vitamin PP 5,0 4.0 6.0 5 Iron sulfate 5.8 5,0 7.0 6 Copper citrate 0.7 0.5 0.9 7 Calcium carbonate 328 300 350 8 Magnesium citrate 210 180 240 9 Sodium thiosulfate 258 240 280 10 Laminaria thallus 300 250 350 eleven Jerusalem artichoke leaf, or root, or a mixture thereof 180 140 200 12 Filler - buckwheat flour Up to 6000 mg

Table 4 Composition 4 No. Name of components The content of the component, mg one 2 3 Denatured collagen proteins of the skin, or cartilage, or ligaments, or tendons, or mixtures thereof one one 3600 3400 3800 2 Vitamin C 180.8 150 200 3 AT 6 09 0.8 1,0 four PP 70 6.0 8.0 5 Iron sulfate 8.8 7.0 10.0 6 Copper citrate 0.8 0.6 1,0 7 Calcium carbonate 375 350 400 8 Magnesium citrate 228.7 200 250 9 Sodium thiosulfate 268 250 300 10 Laminaria thallus 350 300 400 Jerusalem artichoke leaf, or root, gili their mixture eleven 210 150 250 12 Filler - oatmeal Up to 6000 mg

The invention allows to obtain functionally active dietary supplements that eliminate connective tissue deficiency in different categories of people and stimulate the restoration of connective tissue after diseases, injuries and heavy physical exertion.

A dietary supplement (BAA) for food prepared as described in Table 4, column 1, was received by volunteers and skiers (group of 20 people) during training, 8 g 3 times a day. A control group of 20 athletes-skiers did not take a dietary supplement. We have studied the speed of athletes-skiers during their training at a five-kilometer distance at the beginning of the ski season, in the middle of the ski season after 30-day supplement and at the end of the ski season after 60-day supplement. The speed of the athletes at the beginning of the ski season in both groups was approximately the same: in the experimental group it was 10.7 km / h, while in the control group it was 10.8 km / h. After 30 days, the speed of the five-kilometer distance athletes of the experimental group was increased by 11.8%, while in the control group the speed was increased by 6.1%. After 60 days, the speed of the five-kilometer distance athletes of the experimental group was increased by 14.2%, and in the control group the speed was increased by 8.5%, when compared with the speed of the athletes of the five-kilometer distance at the beginning of the ski season. Additional nutrition with biologically active additives allowed skiers to strengthen the ligamentous-articular connective tissue and significantly increase their speed by 5 km after 60 days of training.

Claims (7)

1. A biologically active food supplement based on denatured collagen, including collagen proteins in a denatured form and vitamin C, characterized in that it additionally contains vitamin B6, vitamin PP, calcium carbonate, iron sulfate, copper citrate, magnesium citrate, thallus laminaria, Jerusalem artichoke leaf or root, or a mixture thereof, and filler in the following ratio of components, mg:
Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 1800-3800 Vitamin C 10-200 Vitamin B6 0.1-1.0 Vitamin PP 2.0-8.0 Iron sulfate 1,5-10,0 Copper citrate 0.2-1.0 Calcium carbonate 150-400 Magnesium citrate 75-250 Laminaria thallus 200-400 Jerusalem artichoke leaf or root, or a mixture thereof 100-250 Filler Up to 6000 2. Biologically active food supplement according to claim 1, characterized in that it contains the following ratio of components, mg:
Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 1800-2200 Vitamin C 10-30 Vitamin B6 0.1-0.5 Vitamin PP 2.0-4.0 Iron sulfate 1.5-2.5 Copper citrate 0.2-0.3 Calcium carbonate 150-250 Magnesium citrate 75-120 Laminaria thallus 200-250 Jerusalem artichoke leaf or root, or a mixture thereof 100-150 Filler Up to 6000
and is intended primarily for the nutrition of children. 3. Biologically active food supplement according to claim 1, characterized in that it further comprises sodium thiosulfate in the following ratio of components, mg:
Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 2600-3000 Vitamin C 80-120 Vitamin B6 0.4-0.6 Vitamin PP 3.0-5.0 Iron sulfate 4.0-6.0 Copper citrate 0.4-0.8 Calcium carbonate 250-350 Magnesium citrate 150-200 Sodium thiosulfate 200-250 Laminaria thallus 220-300 Jerusalem artichoke leaf or root, or a mixture thereof 125-175 Filler Up to 6000
and is intended for adults, mainly weakened people. 4. Biologically active food supplement according to claim 1, characterized in that it further comprises sodium thiosulfate in the following ratio of components, mg:
Denatured collagen skin proteins, or cartilage, or ligaments, or tendons, or a mixture thereof 2800-3200 Vitamin C 120-150 Vitamin B6 0.6-0.8 Vitamin PP 4.0-6.0 Iron sulfate 5.0-7.0 Copper citrate 0.5-0.9 Calcium carbonate 300-350 Magnesium citrate 180-240 Sodium thiosulfate 240-280 Laminaria thallus 250-350 Jerusalem artichoke leaf or root, or a mixture thereof 140-200 Filler Up to 6000
and is intended primarily for the nutrition of women. 5. Biologically active food supplement according to claim 1, characterized in that it further comprises sodium thiosulfate in the following ratio of components, mg:
Denatured Collagen Skin Proteins or cartilage, or ligaments, or tendons, or a mixture thereof 3400-3800 Vitamin C 150-200 Vitamin B6 0.8-1.0 Vitamin PP 6.0-8.0 Iron sulfate 7.0-10.0 Copper citrate 0.6-1.0 Calcium carbonate 350-400 Magnesium citrate 200-250 Sodium thiosulfate 250-300 Laminaria thallus 300-400 Jerusalem artichoke leaf or root, or a mixture thereof 150-250 Filler Up to 6000
and is intended primarily for nutrition of men subject to great physical exertion. 6. A biologically active food supplement according to any one of claims 1 to 5, characterized in that it contains rice, or buckwheat, or oat flour as a filler. 7. Biologically active food supplement according to any one of claims 1 to 6, characterized in that it is made in the form of powder, or tablets, or capsules, or jelly, or jelly, or "bars".