KR101883161B1 - Health functional food composition(pill type, paste type product) improving joint health and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a method for preparing a pill for improving a joint health, and a pill for improving a joint health prepared thereby. The method of the present invention comprises the steps of: (1) preparing a chicken foot-tenderizing liquid after adding water to a chicken foot to heat the same; (2) adding protease to the chicken foot-tenderizing liquid prepared in the step (1) to be reacted; (3) heating a chicken foot enzyme reaction liquid which is reacted with an enzyme in the step (2), and filtering and concentrating the same to prepare a chicken foot concentrate; (4) preparing medicinal powder by mixing Achyranthes powder, safflower seed powder, Acanthopanax powder, Eucommia bark powder, cinnamon powder, quince powder, and gelatin of harts horn powder; and (5) preparing a pill with a mixture of mixing the medicinal powder prepared in the step (4) with the chicken foot concentrate prepared in the step (3), and then drying the same.

Description

[0001] The present invention relates to a health functional food composition (a pill, a paste type product) for improving joint health and a method for manufacturing the health functional food composition,

The present invention relates to a process for preparing a chicken broth containing proteolytic enzyme-treated chicken broth concentrate and a pharmaceutical powder comprising a mixture of a starch powder, a safflower powder, a safflower seed powder, an acacia oil powder, a mulberry powder, an egg yolk powder, a neck powder and a starch powder, A method for manufacturing a ring for improving joint health and a ring for improving joint health prepared by the method.

In the human body, joints are the joints of two bones and are surrounded by articular cartilage. Bone is a hard, calcified tissue of calcium and phosphorus that plays an important role in supporting and protecting the body. It maintains a healthy state without loss of articular cartilage, smoothly moving joints, and normal bone breakdown and re- Joint / bone.

The body's bones are a very dynamic organ that breaks up every day and maintains homeostasis by being filled with new bones as much as the disassembled amount. Osteoclasts that break down bones and osteoblasts that regenerate bone maintain homeostasis. RANKL (Receptor Activator of NF-κB Ligand) is an inflammatory precursor protein and an osteoclast surface receptor protein. As the activity of osteoclasts that absorb bone is increased, bone destruction is promoted, bone becomes thin, and diseases such as osteoporosis are easily broken. When the activity of osteoblasts increases, Or bone metastasis. Thus, osteoclast differentiation and increased activity leads to bone destruction such as periodontitis, osteoporosis, arthritis, and the like.

However, modern humans can be harmful to the health of joints / bones due to various reasons such as lack of calcium and protein, hormone imbalance, decrease of physical activity, frequent ingestion of processed foods.

Chickenball is a high-protein nutritional food used as a cooking ingredient in Korea and other parts of Korea. Collagen is one of the major nutrients in chickens, which is good for young women's skin and is also good for osteoporosis, neuralgia and arthritis. . The collagen is also referred to as collagen (collagen) as a light protein (hard protein) constituting the scales of fish, bone, cartilage, teeth, tendons, skin in addition to the skin. It is present as a fibrous solid and has a complex horizontal pattern structure when viewed under an electron microscope. It does not dissolve in water, dilute acid and dilute alkali but when it boils, it becomes gelatin and dissolves. It is difficult to get the action of protease such as trypsin, and it is under the action of collagenase. The constituent amino acids are proline, oxyproline, glycine, glutamic acid and the like, and the content of oxyproline which is not present in other proteins is high.

In addition, chickens have cartilage, which is important for taste and nutrition, as well as proteins such as collagen that make up the skin and joints. The major component of cartilage is gelatin, which is an ingredient that is not synthesized in the body and should be ingested from the outside. The gelatin called gelatin contains chondroitin, which is a physiologically active substance, and is good for neuralgia and arthritis. It helps prevent aging, has a high effect on skin beauty, and is effective for cartilage recovery, regeneration ability, and pain relief It is known.

In addition, it contains linoleic acid component, which is good for preventing and alleviating not only heart disease but also various vascular diseases during ingestion. It contains zinc ingredient and is effective for controlling blood sugar, strengthening diabetes and vigor, and improving immunity. DHA , And EPA. Chitin and chitosan are known to be effective in hemostatic effects through promotion of blood coagulation.

Korean Patent No. 1594137 discloses a health functional food composition which helps joint health, Korean Patent No. 1645917 discloses a herbal medicine useful for joint health and a manufacturing method thereof, The present invention is different from the method for producing the herbal composition for helping the herbal composition.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a method for manufacturing a ring having excellent joint health improving effect by optimizing manufacturing conditions such as sorting, pre- And a method of manufacturing a new type of ring which can expect the effect of improving joint health.

In order to solve the above-described problems, the present invention provides a method for producing chicken breast softening liquid, comprising the steps of: (1) adding water to a chicken breast and heating to prepare chicken breast softening liquid; (2) adding a proteolytic enzyme to the chicken's softening liquid prepared in the step (1) and reacting; (3) preparing a chicken broth concentrate by heating the chicken broth enzyme reaction solution obtained in the step (2), filtering and concentrating the chicken chicken enzyme reaction solution; (4) preparing a pharmaceutical powder by mixing mussel powder, safflower seed powder, acacia powder, mulberry powder, interlocking powder, neck and powder and starch granules; And (5) a step of mixing and concentrating a mixture obtained by mixing the chicken concentrate prepared in the step (3) with the pharmaceutical powder prepared in the step (4), and drying the mixture. ≪ / RTI >

In addition, the present invention provides a ring for improving joint health produced by the above method.

The present invention relates to a method for producing a health-oriented functional material, which is capable of improving the health of joints, which is difficult to ingest as a food of the present invention, and provides an excellent taste and flavor and can be easily ingested . In addition, the method of the present invention is advantageous in that the materials can be pretreated and there is no pungent taste and odor peculiar to the material, so that it is easy to take and the effective ingredient can be enhanced. In addition, it can be expected to be a ready-to-eat food that can be easily stored at room temperature because it is a health food that can improve the joint health when the ring of the present invention is steadily ingested.

Fig. 1 is a graph comparing the rheumatoid factor inhibitory effect of the ring extract.

In order to achieve the object of the present invention,

(1) adding water to chicken feet and then heating to prepare chicken broth softening liquid;

(2) adding a proteolytic enzyme to the chicken's softening liquid prepared in the step (1) and reacting;

(3) preparing a chicken broth concentrate by heating the chicken broth enzyme reaction solution obtained in the step (2), filtering and concentrating the chicken chicken enzyme reaction solution;

(4) preparing a pharmaceutical powder by mixing mussel powder, safflower seed powder, acacia powder, mulberry powder, interlocking powder, neck and powder and starch granules; And

(5) A process for producing a poultice for improving joint health, which comprises preparing a concentrated mixture of the chicken concentrate prepared in the step (3) and the prepared mixture of the pharmaceutical powder prepared in the step (4) .

In the method for manufacturing a ring for improving joint health according to the present invention, the chicken meat softening liquid of step (1) is preferably added to chicken broth at a ratio of 0.8-1.2: 0.8-1.2 (w: v) For 50 to 70 minutes. More preferably, chicken broth can be prepared by adding water at a ratio of 1: 1 (w: v) and heating at 100 DEG C for 60 minutes.

In addition, in the method for producing a ring for improving joint health of the present invention, the step (2) may be carried out by adding a proteolytic enzyme to a chicken fever softening solution at 45 to 55 ° C for 10 to 14 hours, , The proteolytic enzyme may be added to the chicken meat softening solution and reacted at 50 ° C for 12 hours.

The protease is Maxazyme NNP DS and Maxipro BAP, an enzyme product of DSM Food Specialties Protease. The Maxazyme NNP DS originates from Bacillus subtilis and degrades the protein at weakly acidic and neutral pHs and has an activity of 180,000 PC / g. Also, the Maxipro BAP originates from Bacillus licheniformis and degrades the protein at neutral and weak basic pH, and has an activity of 480 DAPU / g.

In the method for producing a ring for improving joint health according to the present invention, the chicken chicken concentrate of step (3) is preferably heated at 80 to 90 ° C for 50 to 70 minutes, filtered, brix. More preferably, the chicken chicken enzyme reaction solution is heated at 85 DEG C for 60 minutes, filtered, and concentrated to 30 brix. It has been possible to produce a chicken broth concentrate through the steps (1) to (3) above by extracting a large amount of an effective ingredient and using the same more effectively for improving joint health.

In the method for manufacturing a ring for improving joint health according to the present invention, the pharmaceutical powder of step (4) is preferably 25 to 35 g of mash powder, 25 to 35 g of safflower seed powder, 25 to 35 g of omega powder, 25 30 g of mash powder, 30 g of safflower seed powder, 30 g of acaffin powder 30-35 g, a mixture of 25-35 g of a ground powder, 25-35 g of a neck and powder and 25-35 g of a green peach granule powder, g, 30 g of mulberry powder, 30 g of the gum powder, 30 g of the neck and powder, and 30 g of the antler powder.

The above-mentioned diagonal bridge refers to a mass of glue formed by dewaxing and thickening the dagger for a long time. There is an advantage that it is more effective when it is used after being processed in the form of a polygonal angle bracket compared to a waxy angle.

In addition, in the method for producing a ring for improving joint health according to the present invention, the step (5) is preferably a step of adding a mixture of 200 to 220 g of the pharmaceutical powder to 65 to 75 g of chicken concentrate, (V / w). More preferably, the mixture is prepared by mixing 70 g of chicken concentrate and 210 g of a pharmaceutical powder, and the mixture is dried at 50 ° C to have a moisture content of 11% (v / w). < / RTI >

The ring made by mixing chicken concentrate with the medicinal powders under the conditions of steps (4) and (5) above is excellent in improving joint health, and has a good flavor of ingredients and no odor, I could.

The method for producing a ring for improving joint health of the present invention, more specifically,

(1) adding water to the chicken leg at a ratio of 0.8-1.2: 0.8-1.2 (w: v), and heating the chicken feather at 90-110 ° C for 50-70 minutes to prepare a chicken fever softening liquid;

(2) adding the proteolytic enzyme to the chicken broth softening solution prepared in the step (1), and allowing the proteolytic enzyme to react at 45 to 55 ° C for 10 to 14 hours;

(3) heating the reaction solution of the chicken chicken enzyme enzyme-reacted in step (2) at 80 to 90 ° C for 50 to 70 minutes, filtering and concentrating the chicken chicken concentrate to 25 to 35 brix;

(4) 25 ~ 35g of mash powder, 25 ~ 35g of safflower seed powder, 25 ~ 35g of ocher powder, 25 ~ 35g of mushroom powder, 25 ~ 35g of mushroom powder, 25 ~ 35g of wood powder and 25 ~ To prepare a pharmaceutical powder; And

(5) A mixture of 200 to 220 g of the prepared pharmaceutical powder prepared in the above step (4) is added to 65 to 75 g of the concentrate of chicken broth prepared in the above step (3), and then the moisture content at 9 to 13 % < / RTI > (v / w)

More specifically,

(1) adding water to chicken feet at a ratio of 1: 1 (w: v), and heating the chicken feet at 100 ° C for 60 minutes to prepare chicken breast softening liquid;

(2) adding a proteolytic enzyme to the chicken meat softening solution prepared in the step (1) and allowing the chicken meat softening solution to react at 50 ° C for 12 hours;

(3) preparing a chicken broth concentrate by heating the chicken chicken enzyme reaction solution obtained in the step (2) above at 85 ° C for 60 minutes, filtering and concentrating the chicken broth at 30 brix;

(4) preparing a pharmaceutical powder by mixing 30 g of powdered mash powder, 30 g of safflower seed powder, 30 g of ocher powder, 30 g of mulberry powder, 30 g of guillotine powder, 30 g of neck and powder, and 30 g of antler powder. And

(5) The mixture of 70 g of the concentrate of chicken broth prepared in the above step (3) and 210 g of the prepared medicinal powder prepared in the above step (4) was mixed to obtain a water content of 11% (v / w) at 50 ° C. Drying step.

The present invention also provides a ring for improving joint health produced by the above method.

Hereinafter, production examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples are illustrative of the present invention, and the content of the present invention is not limited to the following Production Examples and Examples.

Manufacturing example  1. Joint health circle

(1) Water was added to crude chicken broth at a ratio of 1: 1 (w: v) and boiled at 100 ° C for 1 hour to prepare chicken broth softening liquid.

(2) The chicken broth softener prepared in the above step (1) was cooled to 50 ° C, and protease enzymes Maxazyme NNP DS and Maxipro BAP were added in an amount of 0.3% Lt; / RTI > for 1 hour.

(3) The enzyme reaction reaction of the step (2) was heated at 85 ° C for 1 hour to inactivate the enzyme, filtered, and concentrated to 30 brix to prepare chicken concentrate.

(4) After drying, 30 g of powdered mash powder, 30 g of safflower seed powder, 30 g of omega-3 powder, 30 g of mushroom powder, 30 g of egg yolk powder, 30 g of neck and powder and 30 g of starch powder were mixed with 80- Were prepared.

(5) 210 g of the pharmaceutical powder prepared in the above step (4) was mixed with 70 g of the chicken broth concentrate prepared in the above step (3) to prepare a mixture.

(6) The mixture prepared in the above step (5) was converted by using a ventilator, and dried at 50 ° C to a moisture content of 11% (v / w) or less.

Comparative Example  1. Joint health circle

The joint health ring was prepared by the method of Preparation Example 1, except that Maxipro BAP was added at 0.6% to the softening solution without using Maxazyme NNP DS as a protease in step (2) Using the prepared concentrate of chicken breasts, joint health rings were prepared.

Comparative Example  2. Joint Health Insurance

(1) Water was added to crude chicken broth at a ratio of 1: 1 (w: v), and the mixture was heated at 121 ° C for 1 hour, filtered and concentrated to 30 brix to prepare chicken broth concentrate.

(2) After drying, 30 g of powdered mash powder, 30 g of safflower seed powder, 30 g of omega-3 powder, 30 g of mushroom powder, 30 g of egg yolk powder, 30 g of neck and powder, Were prepared.

(3) A mixture of 210 g of the pharmaceutical powder prepared in the above step (2) and 70 g of the concentrate of chicken broth prepared in the above step (1) was diluted with a ventilating machine to obtain a water content of 11% (v / w).

Manufacturing example  2. Prototype products that help joint health

(1) 10% of N-acetyl glucosamine and crystalline cellulose, glycerin and honey were mixed and kneaded with respect to the weight of the mixture of step (5) of Preparation Example 1.

(2) The kneaded kneaded product of step (1) was dried using a ventilator, and dried at 50 ° C. to a moisture content of 11% (v / w) or less.

Example  1. Anti-inflammatory effect on arthritis of the ring according to pretreatment condition of chicken leg

To investigate anti-inflammatory effects of rheumatoid arthritis on ring extracts, DBA / 1J mice (25 g, 6 weeks old, central experimental animals) were administered for 6 weeks. The induction of arthritis was induced by dissolving type 2 collagen (Sigma, USA) in 0.1 M acetic acid at a concentration of 2 mg / ml and emulsifying in Freund's incomplete adjuvant (Sigma, USA). 100 μl was then injected subcutaneously into the right foot of the rat. Seven days after the primary injection, the same amount was added to the tail head, and the control (control) was inoculated with the same amount of physiological saline instead of the sample. On the 21st day after the last inoculation, the individuals that formed periarticular redness, swelling and swelling were selected and examined. For the administration of the ring extract, 500 mg / kg of the ring extract was dissolved in 0.9% physiological saline, filtered through a membrane filter (pore size 0.2 μm, Whatman), and orally administered once a day for 6 weeks.

As a result, the rheumatism index was lower than that of the control group in the crude extract administration group of Preparation Example 1 and Comparative Example, and thus it was confirmed that the ring extract inhibited rheumatoid arthritis, (Fig. 1).

Example  2. Assessment of joint health improvement effect of ring according to pretreatment condition

The joint health improvement effect was evaluated with the rings of Preparation Example 1 and Comparative Examples 1 and 2. The evaluation method was divided into three groups of 30 subjects who felt discomfort and pain in the joints. Each group was given 10 g of the daily dose per day. After 30 days of ingestion, the degree of improvement was evaluated by a 10-point scale Respectively.

Evaluation of joint health improvement Ring type Effect evaluation score Production Example 1 8.6 ± 0.4 Comparative Example 1 7.2 ± 0.8 Comparative Example 2 6.2 ± 1.0

As a result, as shown in Table 1, the ring of Preparation Example 1 showed a high score when compared to the rings of Comparative Examples 1 and 2. Therefore, it was confirmed that using chicken broth concentrate under the conditions of Preparation Example 1 and using in broth production was more effective for joint health than the chicken broth concentrate treated under the conditions of Comparative Examples 1 and 2.

Example  3. Assessment of the joint health improvement effect of the fingers according to the type of ingredients and the ratio

The rings were prepared by the method of Preparation Example 1 and the rings of Comparative Examples 3 to 8 with different materials and compounding ratios were evaluated for the joint health improvement effect (Table 2). The subjects were divided into 7 groups. The subjects were divided into 7 groups. Each group was given 10 g of each day. After 30 days of ingestion, the degree of improvement was evaluated by a 10-point scale.

Type of material and mixing ratio (g) Material type Production Example 1 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Chicken concentrate 70 - 70 70 70 60 80 Edible thickeners - 70 - - - - - Powdered mash 30 40 50 30 20 40 20 Safflower seed powder 30 40 40 30 20 20 40 Ogaki powder 30 40 40 30 20 40 20 Mulberry powder 30 30 - 20 20 40 20 Ground powder 30 30 40 20 30 20 40 Neck and powder 30 30 40 20 30 20 40 Tongue Powder 30 - - 20 30 40 20 Powder of foreshore - - - 20 - - - Virgin powder - - - 20 - - - Licorice powder - - - - 20 - - Hut powder - - - - 20 - - Sum 280 280 280 280 280 280 280

As shown in the following Table 3, a score of 8 or more of the rings of Preparation Example 1 was shown, and the joint health improvement effect was evaluated to be the most excellent. Accordingly, it is considered that the preparation of the pills according to the present invention can be easily administered, and the joint pain can be alleviated, thereby contributing to joint health.

Assessment of joint health improvement effect of fingers according to ingredients and compounding ratio Ring type Effect evaluation score Production Example 1 8.6 ± 0.4 Comparative Example 3 4.6 ± 1.2 Comparative Example 4 5.2 ± 0.6 Comparative Example 5 5.0 ± 1.0 Comparative Example 6 5.8 ± 0.4 Comparative Example 7 6.4 ± 1.0 Comparative Example 8 6.8 ± 0.8

Claims (6)

(1) adding water to the chicken leg at a ratio of 0.8-1.2: 0.8-1.2 (w: v), and heating the chicken feather at 90-110 ° C for 50-70 minutes to prepare chicken broth softening liquid;
(2) adding Maxazyme and Maxipro to the chicken meat softening solution prepared in the step (1), and allowing the mixture to react at 45 to 55 ° C for 10 to 14 hours;
(3) heating the reaction solution of the chicken chicken enzyme enzyme-reacted in step (2) at 80 to 90 ° C for 50 to 70 minutes, filtering and concentrating the chicken chicken concentrate to 25 to 35 brix;
(4) 25 ~ 35g of mash powder, 25 ~ 35g of safflower seed powder, 25 ~ 35g of ocher powder, 25 ~ 35g of mushroom powder, 25 ~ 35g of mushroom powder, 25 ~ 35g of wood powder and 25 ~ To prepare a pharmaceutical powder; And
(5) A mixture of 200 to 220 g of the prepared pharmaceutical powder prepared in the above step (4) is added to 65 to 75 g of the concentrate of chicken broth prepared in the above step (3), and then the moisture content at 9 to 13 % < / RTI > (v / w), based on the total weight of the composition. The joint health improvement according to claim 1, wherein at least one material selected from the group consisting of N-acetyl glucosamine, cellulose, glycerin and honey is further mixed during the mixing of step (5) ≪ / RTI > delete delete delete A joint health improving mouthpiece manufactured by the method of claim 1 or 2.

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