RU2456825C1 - "eravit" biologically active food additive with antioxidant properties and additive preparation method - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to food industry, in particular, to production of biologically active food additives and may be used as a source of luteolin bioflavonoid and vitamins having an antioxidant effect. The biologically active additive contains, wt %: medick hay as a source of luteolin bioflavonoid - 40-60, selenium - 0.002-0.002, distilled water- balance up to 100. 10 ml of medick extract contains 4 g of powdered vitamin mixture as follows: vitamin A -910 mcg; vitamin D₃ -9.0 mcg; vitamin C - 100 mg; vitamin PP - 17.0 mg; vitamin E - 12.7 mg-eq; calcium pantothenate B₅ - 5.0 mg; vitamin B₆ -2.0 mg; vitamin B₁₂ - 1.9 mg; vitamin B₁ - 1.7 mg; folic acid - 0.6 mcg; vitamin K₁ - 0.08 mcg; biotin - 270 mcg; vitamin - B₁₂ - 3.0 mcg; beta-carotene - 10 mg. The method envisages preparation of a solution of medick hay, selenium and distilled water, extraction at a temperature of 180-190°C under a pressure of 6×10⁵ Pa for 3-4 hours. Then one performs the extract separation and introduction of the vitamin mixture in an amount of 4 g per 10 ml of the medick hay extract. One performs thorough stirring during 30 minutes and packing.

EFFECT: invention allows to produce a biologically active food additive with an intensified antioxidant effect and increased content of bioflavonoids.

4 cl, 4 tbl, 2 ex

Description

The invention relates to the food industry, in particular to the production of biologically active food additives, and can be used as a source of bioflavonoid - luteolin and vitamins with antioxidant effects.

Domestic and foreign studies indicate a chronic deficiency in the nutrition of a modern person of biologically active additives (BAA) with antioxidant properties, in particular vitamins and bioflavonoids [Health Food Policy. Federal and regional levels / V.I. Pokrovsky, G.A. Romanenko, V.A. Knyazhev, N.F. Gerasimenko, G.G. Onishchenko, V.A. Tutelyan, V.M. Poznyakovsky // Novosibirsk: Sib. univ. Publishing House, 2002. - 344 p .; Pleshkova N.A. The study of the fundamental characteristics that make up the consumer properties of new forms of biologically active food additives / N.A. Pleshkova // Abstract of dissertation for the degree of candidate of technical sciences. Kemerovo, 2011. - 18 p.].

Therefore, of great importance is the need to develop domestic dietary supplements of antioxidant orientation.

Natural components are of particular relevance when creating dietary supplements, since they are in demand by the consumer.

To create biologically active additives that are a source of natural bioflavonoids, it is recommended to use vegetable raw materials, in particular alfalfa.

The use of alfalfa in the production of dietary supplements makes it possible to use them for a long time without the risk of side effects, and often allows solving the problem of bioflavonoid deficiency.

A biologically active preparation based on alfalfa extract obtained by treating it with an extractant containing a mixture of trace elements salts in the following ratio, mg / kg of plant material: iron - 50.0-300.0, zinc - 50.0-200.0, titanium - 0.5-5.0, copper - 0.5-5.0, cobalt - 0.5-5.0, manganese - 0.5-5.0, chromium - 0.5-1.0, vanadium - 0.5-2.0, molybdenum - 0.5-5.0 (patent No. 2176918). This biologically active preparation was produced by the company “Lutsevita” OJSC (Chelyabinsk region, Troitsk) under the name of Lutsevita dietary supplement (Registration No. 004836.Р.643.09.2002).

The disadvantage of Lucevita is that it does not regulate the content of bioflavonoids, therefore, it is not a source of antioxidants.

The next drawback of Lucevita is the introduction of microelements into it, which does not make it an additive with antioxidant properties, in addition, in its production, the microelement titanium is used, which is not included in the rational nutrition procedure, the recommended levels of food and biologically active substances are approved by the chief state sanitary doctor RF dated July 2, 2004 (No. MP 2.3.1.1915-04).

The disadvantage of Luthevita dietary supplement is that it is used as a source of trace elements, but these substances are not regulated in it, but are used only in production technology.

The next drawback is that Luzaevita is not a source of amino acids (Table 1) and organic acids (Table 2) with a recommended intake of 2 grams per day [Rational diet. Recommended levels of consumption of food and biologically active substances / V.A. Tutelyan, A.K. Baturin, O.A.Vrzhenskaya and others // Method MP2.3.11915-04 from 02.07.2004]. Although on the package it is indicated that Lucevita is used for a deficiency of the above micronutrients of food.

Table 1 presents the amino acid content in dietary supplements Lutsevit.

Table 1 Amino acid Mg / 1 g The recommended average daily rate (No. MP 2.3.1.1915-04) the minimum maximum Adequate intake Upper allowable intake Valine 1.12 1.68 2.5 3.9 Leucine 0.8 1.13 4.6 7.3 Methionine + cystine 0.87 1.45 4.1 6.4 Threonine 0.77 1,04 2,4 3,7 Phenylalanine 1.85 2.2 4.4 6.9 Alanine 2.02 2.97 6.6 10.6 Arganin 1.01 1,60 6.1 9.8 Glycine 1,58 1.94 3,5 5,6 Histidine 0.82 1.05 2.1 3.4

From the data of table 1, it follows that the dietary supplement of Lucevita cannot be a source of amino acids: leucine, methionine + cystine, threonine, alanine, arganine, glycine, histidine, because even their maximum content in dietary supplements does not reach 50% of the recommended adequate level of consumption.

The content of organic acids in dietary supplements Lutsevit are presented in table 2.

table 2 Mg / 1 g The recommended average daily rate (No. MP 2.3.1.1915-04) the minimum maximum Adequate intake Upper allowable intake 0.0134 0,091 500 1500

From the data in table 2 it follows that dietary supplements of Lucevita cannot be a source of organic acids, because the content of these minor food components in it is hundreds of times less than the recommended daily allowance.

It should be noted that the disadvantage of dietary supplements of Luzevita is the heat treatment of plant materials - alfalfa. In the production of Lucevita, the processing of plant materials with an extractant is first carried out by cold extraction at a temperature of not more than 50 ° C for 60 minutes, and then by heating to a temperature of 120-130 ° C under a pressure of 6 × 10 ⁵ Pa - the so-called "gentle" thermal regime treatment, the ratio of plant materials to the extractant is 1: 13.5, which leads to a low yield of bioflavonoids in the resulting dietary supplement, and therefore, Luthevita dietary supplement is not a source of bioflavonoids, which are not regulated in it.

Known biologically active drug based on alfalfa extract (patent No. 2145863) obtained by processing plant material of alfalfa with an extractant containing a mixture of salts of trace elements manganese, barium, tin, cobalt, chromium, vanadium, zinc, iron and copper. This drug is produced by NPO Erakond LLC (Republic of Bashkortostan) under the name Erakond dietary supplement. The dietary supplement is registered by the Ministry of Health of the Russian Federation (registration certificate N000150.Р.643.07.98), is entered in the register of products that have passed state registration (certificate No. 77.99.11.U.9568.10.09). Erakond is recommended to be used as a source of minerals and bioflavonoids - rutin.

Erakond dietary supplement differs from Lucevita in that the extractant contains trace elements in the following ratio, mg / kg of plant material: manganese - 22.0, cobalt - 4.0, chromium - 1.0, vanadium - 1.8, zinc - 700.0 , iron - 1030, copper - 1.0, additionally tin - 0.6, selenium - 1.0, barium - 13.0. There is no titanium and molybdenum in the extractant. The ratio of plant materials and extractant is 1: 8. Extracting the mixture is carried out at a temperature of 150 ° C.

BAA Erakond receive as follows. Alfalfa hay is fed to a chopper of plant materials, where it is crushed to 3-5 mm. To prepare the extractant, steam condensate and solutions of trace elements are used. Plant materials and extractant in a ratio of 1: 8 are placed in a mixer and mixed. The temperature of the extractant is 60-80 ° C. Mixing time 20 minutes. The mixing process is carried out using a mixer. For more efficient mixing, the mixer provides pulp circulation using the built-in pump. The pulp circulation process lasts until the total mixing time has elapsed. The finished pulp from the mixer is pumped to a reactor equipped with a pressure, temperature and safety valve. After loading the pulps, the reactor begins to be heated with steam at a pressure of 5.4 ÷ 5.9 · 10 ⁵ Pa to a temperature of 140-150 ° C for 90 minutes. The extract enters the tank due to the difference in pressure in the reactor and the tank. After centrifugal purification of suspended particles from the container, the extract enters the evaporator. To obtain a powdery and plastic form of the preparation, a 40% concentrated extract is fed to a spray drying plant, where air is supplied with a counter-fluid at a temperature of 250-300 ° C. The dried product is collected in receiving containers.

At the same time, the following components are included in the formulation of dietary supplement Erakond, wt.%:

alfalfa hay 12.5 manganese 0.0022 barium 0.0013 tin 0.000006 cobalt 0,0004 chromium 0.0001 vanadium 0.00018 zinc 0,07 iron 0.1030 copper 0.0001 distilled water up to 100%

or salts of trace elements are taken in the following ratio, mg / kg of plant material: manganese - 22.0, barium - 13.0, tin - 0.6, cobalt - 4.0, chromium - 1.0, vanadium - 1.8, zinc - 700.0, iron - 1030.0, copper - 1.0, with a ratio of plant materials: extractant 1: 8.

A powder or dark brown plastic mass is obtained, which is then packaged, packaged, stored at a temperature of 0-12 ° C, with an air humidity of not more than 75%.

Significant disadvantages of Erakond dietary supplement are the use of a small amount of alfalfa hay in the formulation of 12.5 wt.% Or in the ratio of plant materials: extractant - 1: 8 and extraction temperature 140-150 ° C, extraction time - 90 minutes, which is not enough for output bioflavonoids from alfalfa to extract. Thus, the technological regimes and formulation used lead to a low concentration of biologically active substances, in particular flavonoids, in the resulting dietary supplement.

The disadvantage of Heraconda is the presence of tin and barium salts in it, which are not necessary for the human body [Method MP2.3.11915-04 of 02.07.2004].

It should be noted that Erakond is considered a source of minerals: potassium, calcium, magnesium and zinc (a register of products that have passed state registration).

The potassium content in it is 50.0-60.0 mg / g with a normal intake for an adult per day of 2500-3500 mg / g; calcium 25.0-50.0 mg / g with a consumption rate of 1250-2500 mg / g; magnesium 10.0-15.0 mg / g with a consumption rate of 400-800 mg / g; zinc 1.0-2.0 mg / g with a consumption rate of 12.0-40.0 mg / g (No. MP 2.3.1.1915-04). Therefore, dietary supplement Erakond in recommended doses (1-4 g) provides in potassium by 2.4-9.6%, in calcium - 2-8%, in magnesium - 2.5-10% and zinc - 8.3 -33.2% of the daily needs of an adult.

Thus, Herakond cannot be used as a source of the above minerals.

It should be noted that dietary supplements Erakond declared as a source of bioflavonoids, in particular rutin, which is its drawback, because the content of rutin in it is 3.5 mg per 1 g of dietary supplement with an adequate level of human consumption of 30 mg per day, and the upper allowable level of consumption is 100 mg [Rational nutrition. Recommended levels of intake of food and biologically active substances. / V.A. Tutelian, A.K. Baturin, O.A.Vrzhenskaya and others // Method MP2.3.11915-04 of 02.07.2004].

The low content of rutin bioflavonoid in dietary supplements Erakonda is explained by the low extraction temperature of 140-150 ° C and the extraction time of 90 minutes, which is insufficient to extract bioflavonoids from plant materials. These modes of heat treatment of plant materials lead to a low yield of bioflavonoids from alfalfa to the extract.

For oral administration of Heraconda, it is recommended to add 100 ml of warm boiled water to 20 g of dietary supplements Heracond, mix thoroughly and consume 1-2 teaspoons 2-3 times a day.

Therefore, even with the calculation of the maximum dose of 6 teaspoons (30 ml) per day, rutin enters the human body in the amount of 6.33 mg (21.1% of the daily requirement) at a rate of 30 mg per day [Rational nutrition. Recommended levels of consumption of food and biologically active substances / V.A. Tutelyan, A.K. Baturin, O.A.Vrzhenskaya and others // Method MP2.3.11915-04 from 02.07.2004].

When using Erakond dietary supplement in capsules (2 capsules per 50 ml of water), which is 1 g of Erakond dietary supplement, rutin enters the human body in an amount of 3.5 mg (11.6% of the daily requirement).

When using Erakond dietary supplements even at a dose of 4 g per day (2 incomplete teaspoons) while eating, dissolving in water, tea, milk, juice, rutin enters the human body in an amount of 14 mg (46.7% of the daily requirement) ( Register of products that have passed state registration, p. 4).

Therefore, the disadvantage of dietary supplement Erakond is that it is a weak source of bioflavonoids in recommended doses, because any dietary supplement is recommended to be used in order to fill the deficit of the regulated nutrients in it in the human diet in amounts of 30-100% of the physiological needs of a person.

The next drawback of Erakond dietary supplement is that it regulates only one biologically active substance of antioxidant orientation - rutin.

The closest analogue selected as a prototype of the invention includes dietary supplement Eramin (State Registration Certificate No. 77.99.11.3. U.9568.10.89 of October 14, 2009, TU 9197-001-84518363-09).

The content of bioflavonoids in dietary supplements Eramin in terms of luteolin is (10 mg per 1 g) 2.9 times higher in comparison with dietary supplements Erakond, with a recommended average daily intake of 2 g dietary supplements per day, 200% of the adult's body needs for bioflavonoids is satisfied.

Eramin dietary supplement differs from Erakonda by production technology: a solution is prepared from a dry mixture of alfalfa hay and trace elements with alfalfa hay content of at least 40-60 wt.%, Extracted at a temperature of 180-190 ° C for 5-6 hours.

In addition, the dietary supplement "Eramin" differs from Heraconda in the formulation. Eramin contains metal salts as metal-containing elements: iron sulfate 7-hydrate, zinc sulfate 7-hydrate, copper sulfate 5-hydrate, chromium (3) sulfate 6-hydrate, cobalt sulfate 7-hydrate, manganese sulfate 5-hydrate, ammonium vanadium sulfate , additionally contains ammonium molybdenum in the following ratio, wt.%:

alfalfa hay 40-60 iron sulfate 7-water 0.350-0.450 zinc sulfate 7-water 0.08-0.09 5-manganese sulfate 0.045-0.065 5-hydrous copper sulfate 0.001-0.004 ammonium molybdenum acid 0,0006-0,0012 ammonium vanadium 0,0006-0,0012 cobalt sulfate 7-water 0.0015-0.0025 chromium (3) sulfate 6-water 0,0006-0,0012 distilled water up to 100%

The disadvantage of dietary supplement Eramin is that it regulates only one biologically active substance luteolin, i.e. the use of Eramin does not provide the need for other antioxidants in the human body.

The next disadvantage of dietary supplement Eramin is that the trace elements included in the dietary supplement do not have pronounced antioxidant properties.

A significant drawback of Erakond and Eramin is that the active principles of these dietary supplements are considered to be plant components (luteolin, rutin), which are not recommended for regulation in dietary supplements, because the content of biologically active substances in plant raw materials, in particular sowing alfalfa, can vary widely and depends on many factors: weather conditions, time of collection, shelf life and storage conditions, and others.

The applicant set himself the goal of eliminating these shortcomings, by changing the set of ingredients to develop a new formula of a biologically active antioxidant supplement called Erawit - fortified plant extract, which is a source of bioflavonoid - luteolin, trace element selenium and 14 vitamins.

Carrying out numerous experiments, having tried numerous combinations and variations of the components in the composition, the applicant developed the biologically active additive Erawit with the desired technical result, characterized by the quantitative and qualitative composition of the ingredients, in the dietary supplement formula there are no trace elements (iron sulfate 7-hydrous, zinc sulfate 7-hydrous, copper 5-hydrous sulfate, chromium (3) 6-hydrous sulfate, 7-aqueous cobalt sulfate, 5-hydrous manganese sulfate, vanadium ammonium, ammonium molybdenum), because they do not have an antioxidant effect, the trace element selenium and the vitamin mixture are introduced into the alfalfa extract, the production technology of dietary supplements is changed (the alfalfa extract is not evaporated in an oven, but is obtained in liquid form, which allows to increase the preservation of bioflavonoids).

The technical result consists in enhancing the antioxidant protection of the human body by more than 2 times (the activity of the catalase enzyme, the content of ceruloplasmin) by increasing the content of luteolin bioflavonoid by 25%, removing trace elements from the dietary supplement formula, because they are not antioxidants, and the introduction of new biologically active substances of antioxidant orientation in the formula: trace element selenium, vitamin C, vitamin E, beta-carotene. With the recommended average daily intake of 10 ml of dietary supplements per day, 250% is satisfied (with the use of Eramin dietary supplements - 200%) of the adult's daily requirement for bioflavonoids, antioxidants: selenium - 97%, vitamin C - 111%, vitamin E - 84.7 %, beta-carotene - 200%, which does not exceed the upper allowable level of consumption.

The claimed technical result is achieved due to the fact that the biologically active additive contains alfalfa hay, which is a source of bioflavonoid-luteolin, selenium, vitamins and distilled water in the following ratio of components, wt.%:

alfalfa hay no less 40-60 Selena 0.001-0.002 distilled water up to 100%

moreover, 10 ml of alfalfa extract contains vitamins in the form of dissolved biologically active substances in 4 g of powder: vitamin A (retinol), mcg - 910; vitamin D3, mcg - 9; vitamin C (ascorbic acid), mg - 100; vitamin PP (nicotinamide), mg - 17.0; vitamin E (tocopherol), mg.eq. - 12.7; calcium pantothenate B5, mg - 5.0; vitamin B6 (pyridoxine), mg - 2.0; vitamin B2 (riboflavin), mg - 1.9; vitamin B1 (thiamine), mg - 1.7; folic acid, mcg - 0.6; vitamin K1, mcg - 0.08; biotin, mcg - 270; vitamin B12 (cyancobalamin), mcg - 3; beta-carotene, mg - 10.

A method of preparing a biologically active additive by preparing a solution of hay alfalfa seed, selenium and distilled water with alfalfa hay content of at least 40 wt.%, Extraction at a temperature of 180-190 ° C and a pressure of 6 × 10 ⁵ Pa for a duration of 3-4 hours, separation extract and introducing a vitamin mixture into it, characterized in that a solution is prepared from alfalfa hay, selenium and distilled water, and after extraction and subsequent separation, the extract is obtained in liquid form, cooled, where vitamin C is then added es the rate of 4 g per 10 ml of extract alfalfa, stirred thoroughly for 30 minutes using the built-in capacity automatic mixer and packaged.

In addition, the extraction is carried out at a temperature of 180-190 ° C, at a pressure of 6 × 10 ⁵ Pa, lasting 3-4 hours.

In addition, the biologically active additive is cooled to 20-24 ° C and the multivitamin mixture powder is added with the content of biologically active substances in 4 g of powder: vitamin A (retinol), μg - 910; vitamin D3, mcg - 9; vitamin C (ascorbic acid) - 100; vitamin PP (nicotinamide), mg - 17.0; vitamin E (tocopherol) mg.eq. - 12.7; calcium pantothenate B5, mg - 5.0; vitamin B6 (pyridoxine), mg - 2.0; vitamin B2 (riboflavin), mg - 1.9; vitamin B1 (thiamine), mg - 1.7; folic acid, mcg - 0.6; vitamin K1, mcg - 0.08; biotin, mcg - 270; vitamin B12 (cyancobalamin), mcg - 3; beta-carotene, mg - 10.

In addition, the final product is Packed in dark glass bottles with a capacity of 100 ml, approved for contact with food by Rospotrebnadzor, and stored at a temperature not exceeding 25 ° C and relative humidity not more than 75%.

The production technology and the formula of the “Eramin” dietary supplement include the preparation and weighing of plant materials and selenium, the preparation of a solution from a dry mixture of alfalfa hay with alfalfa hay content of 40-60 wt.%, Selenium 0.001-0.002 wt.% And distilled water up to 100% , extraction at a temperature of 180-190 ° C, at a pressure of 6 × 10 ⁵ Pa for 3-4 hours, separation, cooling to 20-24 ° C and adding a multivitamin mixture powder called Santavit (state registration certificate No. 77.99. 23.3.U.6627.8.07 of 08.17.2007 at based on the expert opinion of the Federal State Health Institution “Center for Hygiene and Epidemiology in the City of Moscow” No. 23-198 / B-07 dated May 29, 2007) with the content of biologically active substances in 4 g of powder: vitamin A (retinol), mcg - 910; vitamin D3, mcg - 9; vitamin C (ascorbic acid), mg - 100 vitamin PP (nicotinamide), mg - 17.0; vitamin E (tocopherol), mg.eq. - 12.7; calcium pantothenate B5, mg - 5.0; vitamin B6 (pyridoxine), mg - 2.0; vitamin B2 (riboflavin), mg - 1.9; vitamin B1 (thiamine), mg - 1.7; folic acid, mcg - 0.6; vitamin K1, mcg - 0.08; biotin, mcg - 270; vitamin B12 (cyancobalamin), mcg - 3; beta-carotene, mg - 10.

The multivitamin mixture is added at the rate of 4 g per 10 ml of alfalfa extract, mixed thoroughly for 30 minutes using the automatic mixer integrated in the tank, the extract is fed to the filling line.

The products are packaged in dark glass bottles with a capacity of 100 ml, approved for contact with food products of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare, and stored at a temperature not exceeding 25 ° C and relative humidity not more than 75%.

This technical result is achieved in that the biologically active additive “Erawit” contains a biologically active substance with an antioxidant effect: bioflavonoid luteolin, selenium, ascorbic acid (vitamin C) and vitamin E (tocopherol acetate), beta-carotene, and other vitamins : A (retinol), B1 (thiamine), B2 (riboflavin), B6 (pyridoxine), B12 (cyancobalamin), D3, K1, H (biotin), PP (nicotinamide), B5 (calcium pantothenate), folic acid, beta β-carotene, according to the invention, not only luteolin, but also selenium is used as antioxidants, vitamins.

Table 3 presents the content of biologically active substances in dietary supplements Eramin in 2 g (recommended average daily dose) and Erawit in 10 ml (recommended average daily dose).

Table 3 Biologically active substance BAA Eramin (prototype) Dietary supplement Erawit Content % of the recommended daily requirement for an adult Content % of the recommended daily requirement for an adult bioflavonoids in terms of luteolin twenty 200 25 250 Selenium, mcg 68 97 Vitamin A (retinol), mcg ret. eq. 91 101 vitamin D3, mcg - - 0.009 90 vitamin C (ascorbic acid), mg one hundred 111 vitamin PP (nicotinamide), mg 17.0 85 vitamin E (tocopherol), mg.eq. 12.7 84.7 calcium pantothenate B5, mg 5,0 one hundred vitamin B6 (pyridoxine), mg 2.0 one hundred vitamin B2 (riboflavin), mg 1.9 106 vitamin B1 (thiamine), mg 1.7 113 folic acid, mcg - - 600 66.7 vitamin K1, mcg - - 80 66.7 biotin, mcg - - 270 thirty vitamin B12 (cyancobalamin), mcg 3 one hundred beta-carotene, mg - - 10 200

Due to the fact that to obtain the biologically active additive “Erawit” a solution is prepared from a dry mixture of alfalfa hay with alfalfa hay content of at least 40-60 wt.%, Selenium and distilled water, it is extracted at a temperature of 180-190 ° C 3-4 h, cool the extract to room temperature and inject the vitamin mixture (biologically active substances of antioxidant orientation (vitamin E, ascorbic acid, beta-carotene), with the recommended average daily intake of 10 ml of dietary supplement per day, the average daily requirement of the body is satisfied zroslogo person antioxidants: Luteolin 250%, selenium - 97%, vitamin C - 111%, vitamin E - 84.7%, which allows to enhance antioxidant defense of the human body (enzyme catalase activity, the content of ceruloplasmin and immunoglobulins).

All the technology and the formulation for obtaining dietary supplements Erawit with the claimed technical result is selected in detail and strictly verified by the applicant by changing the technological parameters (lack of drying of alfalfa extract) and prescription components. Thanks to this selection of components in the composition, the necessary technical result is achieved.

Comparative analysis with the prototype allows us to conclude that the claimed dietary supplement differs from the known absence of the process of evaporation of alfalfa extract, which allows to increase the preservation of bioflavonoids, and the dietary supplement formula differs from the known new qualitative composition of the components.

The set of essential features of the claimed technical solution, technology and composition do not follow explicitly from the studied prior art, which meets the criteria of "novelty" and "inventive step".

The claimed dietary supplement "Erawit" will be widely used in the food industry, i.e. composition and technology are industrially applicable.

The production of dietary supplements has the following stages:

- preparation of plant materials (quality control: organoleptic, physico-chemical indicators, safety criteria; degree of grinding of dry alfalfa leaves up to 5-8 mm - standard particle size when grinding medicinal plant materials (LRS);

- weighing of plant materials - alfalfa hay, microelement of selenium;

- preparation of a solution of alfalfa, selenium and distilled water;

- extraction (T = 180-190 ° C, pressure 6 × 10 ⁵ Pa, duration 3-4 hours);

- separation of the extract;

- cooling the extract to T 20-25 ° C;

- the introduction of a vitamin mixture and mixing for 30 minutes;

- packing in dark glass bottles of 100 ml;

- packaging (a bottle with instructions for use is placed in a pack of cardboard).

The invention is illustrated by examples.

Example 1

The basis for obtaining dietary supplements "Eramin" is hydrobarothermic processing of plant materials.

Prepare a solution in the following ratio of the components of the formulation, wt.%:

alfalfa hay 40-60 selenium 0.001-0.002 distilled water up to 100%

(so that the mass of the solution reaches 100%).

The prepared solution is placed in an autoclave, sealed in an autoclave, heating the autoclave to a temperature of 180-190 ° C, lasting 3-4 hours at a pressure of 6 × 10 ⁵ Pa, cooled to a temperature of 60 ° C, incubated for 30 minutes at a pressure of 6 × 10 ⁵ Pa The autoclave is depressurized, the extract is poured into a storage tank.

The extract enters the separator. After separation, the extract is pumped to the drive, from which it is fed into the tank.

A vitamin mixture with the content of biologically active substances in 4 g of powder is introduced into the obtained extract: vitamin A (retinol), mcg - 910; vitamin D3, mcg - 9; vitamin C (ascorbic acid), mg - 100; vitamin PP (nicotinamide), mg - 17.0; vitamin E (tocopherol), mg.eq. - 12.7; calcium pantothenate B5, mg - 5.0; vitamin B6 (pyridoxine), mg - 2.0; vitamin B2 (riboflavin), mg - 1.9; vitamin B1 (thiamine), mg - 1.7; folic acid, mcg - 0.6; vitamin K1, mcg - 0.08; biotin, mcg - 270; vitamin B12 (cyancobalamin), mcg - 3; beta-carotene, mg - 10.

The multivitamin mixture is added at the rate of 4 g per 10 ml of the extract, everything is thoroughly mixed for 30 minutes using the automatic mixer integrated in the tank, the extract is fed to the filling line.

BAA "Erawit" is a dark brown liquid. The taste is sweet and sour.

BAA "Erawit" is released in the form of a solution in dark glass bottles with a capacity of 100 ml.

Example 2. For the production of dietary supplements "Erawit" prepare a solution in the following ratio of components, wt.%: Alfalfa hay 50, selenium 0.0015, distilled water to 100% (so that the mass of the solution reaches 100%).

The rest of the process is carried out as in example 1.

Example 3. For the production of dietary supplements "Erawit" prepare a solution in the following ratio of components, wt.%:

alfalfa hay 60 selenium 0.002 distilled water up to 100%

(so that the mass of the solution reaches 100%).

The rest of the process is carried out as in example 1.

Dietary supplement Erawit has strong antioxidant activity, which is explained by a high content of luteolin bioflavonoid and selenium trace element, which stimulate the non-enzymatic link of the body's antioxidant system, as well as the presence of vitamins, which themselves represent the enzymatic link of the body's antioxidant defense.

In every human cell, there is a protective mechanism that prevents excessive accumulation of lipid peroxidation products - this is an antioxidant system. According to modern scientific concepts, this system consists of two links:

- enzymatic, represented by oxyreductase (glutathione reductase, etc.) and antiperoxid (catalase (K), superoxide dismutase, etc.) and enzymes;

- non-enzymatic, represented by water-soluble vitamins, proteins and other compounds.

Luteolin and selenium prevent abnormalities in DNA molecules, enzymes, polyunsaturated fatty acids and other compounds through radical-radical interactions.

Luteolin and selenium enhance the activity of oxidoreductase (glutathione reductase) and antiperoxidase enzymes (catalase), increase the content of proteins of immunoglobulins and ceruloplasmin (CP), which protect lipid membranes from peroxidation and have the activity of oxidoreductase enzymes, which are the main superoxide oxidase, and glutathione oxidase plasma. Using pairs of copper (II) ions, the CP binds a superoxide radical ion and restores oxygen to water.

In connection with the foregoing, the evaluation of the antioxidant activity of dietary supplements Erakond, dietary supplements Eramin and dietary supplements Erawit was carried out according to the following indicators: the enzyme catalase (K) and ceruloplasmin (CP).

To study the effect of dietary supplements on the antioxidant activity of the body, 4 groups of laboratory animals (white outbred mice with a live weight of 20-22 g) were formed on the basis of analogues of 10 mice in each group. The first group (control) - animals of this group did not receive dietary supplements. White mice of the second experimental group were injected via a probe into the dietary supplement Erakond, after dissolving the powder of dietary supplement (4 g) in water, twice a day for 30 days according to the recommendation for use. Animals of the third group were administered dietary supplement Eramin 1 g twice a day for 20 days (according to the recommendation for use). White mice of the fourth group - dietary supplement Erawit was administered orally at a dose of 10 ml of solution for 10 days.

Activity K was determined by the generally accepted method based on the decomposition of hydrogen peroxide by catalase and the determination of the light absorption of the complex with ammonium molybdate at a wavelength of 410 nm.

Determination of CP in serum - according to the modified method of S.V. Bestuzheva, V.G. Kolb (1976).

The studies were carried out on the basis of the inter-department laboratory of the UGAVM.

The obtained materials were processed by the method of variation statistics using Student's criterion and the Biostat computer program.

Table 4 presents the effect of dietary supplements on the antioxidant protection of experimental animals.

Table 4 The activity of the catalase enzyme and the content of ceruloplasmin in the blood serum of white mice in the control group and taking dietary supplements Group 1 (control) 2 (dietary supplement Erakond) 3 (dietary supplement Eramin) 4 (dietary supplement Erawit) K
nmol H ₂ O ₂ / mg protein CPU
mg% K nmol H ₂ O ₂ / mg protein CPU
mg% K nmol H ₂ O ₂ / mg protein CPU
mg% K nmol H ₂ O ₂ / mg protein CPU
mg% Before taking dietary supplements 618.1 ± 8.9 16.7 ± 1.2 617.5 ± 7.5 17.5 ± 0.5 610.5 ± 9.5 16.2 ± 0.3 605.6 ± 9.4 15.9 ± 0.3 After taking dietary supplements 620.7 ± 9.3 18.5 ± 2.4 691.7 ± 6.5 19.1 ± 0.2 * 695.1 ± 8.1 * 18.5 ± 0.2 * 843.4 ± 8.2 ** 20.5 ± 0.2 ** * P≤0.05; ** P≤0.01

From the data in table 3 it is seen that, against the background of the use of dietary supplements Erawit, the activity of catalase and the content of ceruloplasmin in the blood of mice of the fourth group significantly increased by 39.3% and 28.9%, respectively, while in the second by 12.0% and 9, one%. In the third group, a change in these indicators upward amounted to 13.9% and 14.2%. In the blood of animals of the control group, no significant changes in the activity of catalase and ceruloplasmin were noted.

Thus, the antioxidant effect of dietary supplements Erawita is more than 2 times more effective in comparison with its analogs dietary supplements Erakond and dietary supplements Eramin.

Claims (4)

1. A biologically active food supplement, characterized in that it contains alfalfa hay, which is a source of bioflavonoid - luteolin, selenium, vitamins and distilled water in the following ratio of components, wt.%:
alfalfa hay 40-60 selenium 0.001-0.002 distilled water up to 100%
moreover, 10 ml of alfalfa extract contains vitamins in the form of dissolved biologically active substances in 4 g of powder: vitamin A (retinol), mcg - 910; vitamin D3, mcg - 9; vitamin C (ascorbic acid), mg - 100; vitamin PP (nicotinamide), mg - 17.0; vitamin E (tocopherol), mg equiv. - 12.7; calcium pantothenate B5, mg - 5.0; vitamin B6 (pyridoxine), mg - 2.0; vitamin B2 (riboflavin), mg - 1.9; vitamin B1 (thiamine), mg - 1.7; folic acid, mcg - 0.6; vitamin K1, mcg - 0.08; biotin, mcg - 270; vitamin B12 (cyancobalamin), mcg - 3; beta-carotene, mg - 10. 2. A method of preparing a biologically active additive, characterized by the preparation of a solution of sowing alfalfa hay, selenium and distilled water with alfalfa hay content of 40-60 wt.%, Extraction at a temperature of 180-190 ° C and a pressure of 6 · 10 ⁵ Pa, lasting 3- 4 hours, separating the extract and introducing the vitamin mixture into it, and after extraction and subsequent separation, the extract is obtained in liquid form, cooled, where the vitamin mixture is then added at the rate of 4 g per 10 ml of alfalfa extract, mixed thoroughly for е 30 min with the help of the built-in automatic mixer in the container and pack. 3. The method of preparing the biologically active additive according to claim 2, characterized in that the biologically active additive is cooled to 20-24 ° C and a multivitamin mixture powder is added with the content of biologically active substances in 4 g of powder: vitamin A (retinol), μg - 910 ; vitamin D3, mcg - 9; vitamin C (ascorbic acid), mg - 100; vitamin PP (nicotinamide), mg - 17.0; vitamin E (tocopherol), mg equiv. - 12.7; calcium pantothenate B5, mg - 5.0; vitamin B6 (pyridoxine), mg - 2.0; vitamin B2 (riboflavin), mg - 1.9; vitamin B1 (thiamine), mg - 1.7; folic acid, mcg - 0.6; vitamin K1, mcg - 0.08; biotin, mcg - 270; vitamin B12 (cyancobalamin), mcg - 3; beta-carotene, mg - 10. 4. The method of preparation of the biologically active additives according to claim 2, characterized in that the final product is packaged in dark glass bottles with a capacity of 100 ml, approved for contact with food products of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare, and stored at a temperature not exceeding 25 ° C and relative humidity more than 75%.