KR20040081534A - Method for manufacturing the health food - Google Patents

Abstract

PURPOSE: A method of making a health supplementary food product using an extract of a mushroom cultivated in the culture medium containing selenium and a preservative is provided. The food product is harmless to the human body and contains selenium as an essential mineral and is thus useful for maintaining good health. CONSTITUTION: The mushroom cultivated in the culture medium containing selenium is heated, ground and then extracted at a temperature of 40 to 100deg.C for 10 to 6,000min. Then, 1L of the obtained mushroom extract is agitated with 10 to 1,000g of a preservative for 1 to 5hr and then diluted in a dilution ratio of 10 to 1,000 times. The mushroom contains 100 to 1,000μ g/100g. The preservative is any one of benzoic acid, sodium benzoate, dehydroacetic acid, sorbic acid, p-hydroxybenzoate ester, sodium propionate or calcium salt. The mushroom is at least one selected from Flammulina velutipes, Pleurotus ostreatus, Phellinus linteus and Agaricus bisporus mushroom.

Description

Method for manufacturing the health food

The present invention relates to a method for producing a dietary supplement, and more particularly, to a method for preparing a dietary supplement that is capable of supplying minerals essential to the human body by mixing an extract of a mushroom and a preservative.

In general, selenium is a trace mineral essential to the human body and is an important component of antioxidant enzymes that protect cells from free radicals produced during the metabolism of oxygen-consuming metabolism, and also the immune system and thyroid. gland) is essential for normal functioning.

In particular, deficiency of selenium results in Kehan's disease, which is a heart disease, or Kasin-Beck, a joint disease.

The recommended daily intake of selenium is 55 ㎍ in adult, 60 ㎍ in pregnant women and 70 ㎍ in lactation period. The insufficiency of selenium is associated with low selenium inhabitants and people with weakened selenium absorption due to gastrointestinal dysfunction. This has been observed in patients who have received nutritional supplements that do not contain selenium.

Supplementation of selenium in people who do not have enough selenium intake can boost the immune system (Baum MK, et al Selenium and interleukins in persons infected with human immunodeficiency virus type 1.J Infect Dis. 2000; 182 Suppl 1: S69- 73) and anti-cancer effects (Combs GF, Jr., Gray WP. Chemopreventive agents: selenium. Pharmacol Ther. 1998; 79 (3): 179-192).

Excessive intake of selenium has side effects such as gastrointestinal disorders and neurological disorders due to selenium toxicity, so the maximum daily intake is limited to 400 µg. As a supplemental supply form of selenium, selenium inorganic substances, such as sodium selenite and sodium selenate, and selenium organic substances, such as seleno methionine, are used.

Selenomethionine is a natural organic compound of selenium found in natural foods. To date, most products have been supplied by converting selenium minerals into yeast to selenomethionine (Fan et al US Pat. No. 6,368,643). .

Selenate is absorbed almost entirely, but is characterized by a significant amount of urine excretion before binding to protein. In addition, selenite absorbs only about 51%, but the degree of retention in the body is superior to selenate, which accounts for most of the selenium mineral feed.

Yeast Selenomethionine has a 57% absorption and retention rate superior to selenite (Pagan JD et al Effect of selenium source on selenium digestibility and retention in exercised throughbreds. 1999 Proc. 16th Equine Nutrition and Physiology Society) It may contain strong inorganic selenium and therefore draws attention to its use.

Most of the selenium in natural foods is in the form of selenomethionine, which is the most desirable source of selenium because the absorption rate of selenium methionine, a natural selenium organic substance, is 90% or more and safer than that of inorganic selenium. Board, Institute of Medicine.Selenium.Dietary reference intakes for vitamin C, vitamin E, selenium, and carotenoids.Washinton DC: National Academy Press; 2000: 284-324).

The present invention has been made to solve the problems as described above, the method of producing a dietary supplement that can supply the minerals essential to the human body by mixing the extract and preservatives of mushrooms, which is a natural food containing selenium The purpose is to provide.

In order to achieve the above object, the present invention, by charging the selenium-containing mushroom powder in a hot water extractor for 10-6000 minutes in the temperature range of 40 ~ 100 ℃ to extract the hot water extract, hot water in the mixing tank After charging the extract, charge 10 to 100 g of a preservative in a weight ratio to 1 liter of this charge and stir for 1 to 5 hours to prepare a composition stock solution.The composition stock solution is then added to beverages such as water or milk, soy milk, and lactic acid bacteria beverages. It is characterized by diluting at a ratio of -1000 times.

In addition, the present invention for achieving the above object, charged with a selenium-containing mushroom powder in a hot water extractor heated for 10-6000 minutes in a temperature range of 40 ~ 100 ℃ to extract the hot water extract, then mixed After charging the hot water extract to the tank, charge 10 to 100 g of preservative in a weight ratio to 1 liter of the charge and stir for 1 to 5 hours to prepare the composition stock solution, and then dry powder the composition stock solution and provide it as a capsule formulation. It features.

Natural foods that can be used as an extract material of selenium in the present invention, there is a relatively high content of selenium (shrimp), salmon (salmon), tuna (tuna), brazil nut, garlic and the like.

In particular, Brazil nuts have a selenium content of 840 µg / oz, higher than other foods (US Department of Agriculture, Agricultural Research Service.Nutrient Database for Standard Reference, Release 12 .: Nutrient Data Lab Home Page. URL 1998). Although preferred as an extraction material, the present invention provides a source of natural selenium organic matter that is more easily supplied.

The present invention is a selenium-containing mushroom by cultivating the mushrooms using the selenium purified stock solution prepared by the apparatus and method for producing functional water containing useful minerals such as selenium (Korean Patent Application No. 2002-57024) It provides a method to use as a source of organic matter.

Hereinafter, the manufacturing method of the health supplement food according to the present invention will be described in detail with reference to Examples.

First, one to several kinds of mushrooms, such as oyster mushrooms, enoki mushrooms, mushroom mushrooms, and situation mushrooms, grown in a medium containing 50-500 mg / l purified stock solution of selenium, are subjected to natural ventilation drying or heat drying. Cut into 1-2mm flakes or crushed by pressing with a press.

At this time, the mushrooms used as raw materials should be limited to those having a selenium content in the range of 100 to 1000 µg / 100 g. This is because a mushroom having a selenium content of 100 µg / 100g or less has low extraction efficiency, and thus, it is difficult to obtain the effects of the present invention.

The raw material treated according to the above method is charged into a hot water extractor and extracted for 10 minutes to 6000 minutes with hot water of 40 to 100 ° C. The extraction conditions are preferably 60 to 100 ° C and 30 minutes to 3000 minutes, more preferably 70 It is -90 degreeC and 120 minutes-600 minutes.

At this time, as the extraction temperature range of the mushroom, the extraction efficiency of selenium is low at 40 ° C or lower, while other nutrients of the mushroom are oxidized at 100 ° C or higher. As extraction time, extraction efficiency is low in 10 minutes or less, and productivity falls in 6000 minutes or more.

As a method of extracting selenium from mushrooms for implementing the composition of the present invention, other methods, such as extraction using protein enzymes well known in the food industry, are possible in addition to the hot water extraction method.

After extracting the hydrothermally treated extract into a mixing tank and adding components such as a preservative to ensure storage properties, the mixture is stirred and mixed at room temperature for 1 to 5 hours.

At this time, in the mixing process of the mixing tank, other additives such as sweeteners, fragrances may be input treatment. The mixture is provided as a liquid composition stock solution in the case of liquid formulations and concentrated to dryness under reduced pressure in the case of capsule formulations.

The composition of the present invention may be formulated in the form of powders, tablets, liquids, capsules, pills, etc. by methods commonly used in the food and pharmaceutical industries. In the present invention, examples include liquid drinks and capsules. It is not intended to limit the formulation.

Water or fruit, vegetable drinks (concentrated fruit juice, concentrated vegetable juice, concentrated fruit and vegetable juice, fruit juice, vegetable juice, fruit drink, vegetable beverage, fruit and vegetable drinks), carbonated beverages Carbonated beverages, carbonated water, flavored carbonated beverages, soy milk (soy milk solution, soy milk, prepared soy milk, powdered soy milk, other soy milk), fermented beverages (lactic acid bacteria, food, yeast beverage, other fermented beverages), powdered beverages, other beverages (extraction) Beverages, beverage base), alcoholic beverages, teas, herbal beverages (ginseng beverages), including a dilution with a beverage that can be used as a food to provide a mushroom extract-containing beverage of the present invention.

In addition, the amount of the mushroom extract contained in the composition of this invention is 0.02-2.0% (solid content 0.1-10%) by weight. Preferably it is 0.05 to 0.8%, More preferably, it is 0.1 to 0.6%. Concentrations of selenium are not expected at concentrations below 0.02% and concentrations above 2.0% may exceed the maximum daily intake of selenium.

The addition amount of the preservative contained in the composition of this invention is 0.0002 to 0.2% by weight. In 0.0002% or less, the effect of preservation is insufficient. In the case of 0.2% or more, the effect of increasing the preservation function is insignificant.

As the preservative, benzoic acid, sodium benzoate, dehydroacetic acid, sorbic acid, and paraoxybenzoic acid esters sodium propionate and calcium salt can be used, and the amount of each added is in accordance with the food and drug safety regulations of each country including the Republic of Korea within the above amount. .

As a possible additional ingredient of the present invention, other food ingredients such as royal jelly, vitamins, minerals, etc., may be added to enhance health.

Example 1

Imported inspection of the top mushrooms grown with a medium containing selenium 500 mg / l purified stock solution to remove foreign substances, dried at 110 ℃, powdered with a grinder, charged into a hydrothermal extractor and extracted for 6 hours under 80 ℃ hot water. After the extract was transferred to the mixing bath. 250 g of sodium benzoate per liter of hot water extract was added to a mixing tank and mixed for 3 hours. The final composition was prepared by diluting water 100-fold in the composition stock solution where mixing was completed.

As a result of analyzing the selenium content of the composition stock solution according to the amount of raw material of the top mushroom powder by HPLC, it was confirmed that the selenium content was linearly directly proportional to the amount of raw material. Got it.

The selenium content of the final composition obtained by diluting the composition stock solution 100 times with water in the range of 0.02 to 2.0% (solid content of 0.1 to 10%) by weight was 1.0 to 100 µg / 100 ml. do.

Example 2

Imported inspection of the top mushroom and oyster mushroom cultivated with medium containing selenium 300mg / l purified stock solution to remove foreign substances, heat it to 110 ℃, powder it with a grinder and charge the powder into a hydrothermal extractor for 3 hours under 80 ℃ hot water After extraction, the extract was transferred to a mixing bath. 50 g of sodium benzoate per liter of the mushroom hot water extract was added to a mixing tank and mixed for 1 hour. The final composition was prepared by diluting the mixture stock solution 100 times with lactic acid bacteria beverage.

As a result of analyzing the selenium content of the composition stock solution according to the amount of the raw material of the mushroom powder, it was confirmed that the selenium content was linearly directly proportional to the amount of the raw material and the extraction result of 900 ± 250㎍ of selenium per 100g weight was obtained. .

The selenium content of the final composition obtained by diluting the above composition stock solution 100 times with lactic acid bacteria beverage in the amount of the extract of the mushroom in the range of 0.02 to 2.0% (solid content of 0.1 to 10%) by weight was 0.9 to 90 µg / 100 ml. Becomes

Example 3

Import and examine the top mushrooms and situation mushrooms grown in medium containing selenium 500 mg / l purified stock solution, remove foreign substances, heat them to 110 ℃, dry them into a grinder and powder them into a hydrothermal extractor. After extracting for a time, the extract was transferred to a mixing bath. Sodium benzoate was added to 100 g per 1 liter of mushroom hot water extract in a mixing bath and mixed for 2 hours. The final composition was prepared by diluting the composition stock solution 100 times with milk.

As a result of analyzing the selenium content of the composition stock solution according to the amount of raw material of the mushroom powder, it was confirmed that the selenium content was linearly proportional to the amount of raw material and the extraction result of 800 ± 100 ㎍ of selenium per 100 g of weight was obtained. .

Selenium content of the final composition obtained by diluting the composition stock solution 100 times with lactic acid bacteria drink in the range of 0.02 to 2.0% (solid content 0.1 to 10%) by weight based on weight, was 0.8 to 80 µg / 100 ml. Becomes

Example 4

Imported inspection of the top mushroom, cultivated mushroom and mushroom mushroom cultivated in the medium containing selenium 200 mg / l purified stock solution, removing foreign substances, heating to 110 ℃, powdered with a pulverizer and charging the powder into a hot water extractor After hot water extraction for 10 hours, the mixture was transferred to a mixing bath.

250 g of sodium benzoate per 1 liter of the mushroom hot water extract was added to a mixing tank and mixed for 5 hours. The final composition was prepared by diluting water by adding 100 times the composition stock solution to which mixing was completed.

As a result of analyzing the selenium content of the composition stock solution according to the amount of the raw material of the mushroom by the instrumental analysis of HPLC, it was confirmed that the selenium content according to the amount of the raw material was directly proportional to each other, and the extraction result of 900 ± 150㎍ of selenium per 100g weight.

The amount of the extract of the mushrooms is 0.9-90 µg / 100 ml of the final composition obtained by diluting the composition stock solution 100 times with water in the range of 0.02-2.0% (solid content 0.1-10%) by weight. .

Example 5

Imported inspection of top mushrooms and oyster mushrooms grown with medium containing selenium 400 mg / l purified stock solution to remove foreign matters, and then heated to 110 ℃, powdered with a pulverizer and charged into a hydrothermal extractor. After extraction for time, the mixture was transferred to a mixing bath.

250 g of sodium benzoate per 1 liter of the mushroom hot water extract was added to a mixing tank and mixed for 5 hours.

The composition stock solution after mixing was heated to 110 ° C. and finally powdered with a grinder.

As a result of analyzing the selenium content of the final powder according to the amount of mushroom raw material by HPLC instrumentation, it was confirmed that the selenium content according to the raw material amount was linearly proportional to each other and the extraction result of 1000 ± 200 ㎍ of selenium per 100 g of weight was obtained.

The selenium content of the final powder is provided in a 500 mg dosage form into a capsule is 35 μg / piece.

As described above, according to the manufacturing method of the health supplement food according to the present invention, by using natural food as the main raw material to provide selenium, which is an essential trace mineral of the human body as a supplement or drink to help the human body while maintaining health It can be used to contribute to the improvement of public health, and especially to prevent and treat selenium deficiency diseases.

Claims (5)

The mushrooms cultivated in the medium containing the selenium purified stock solution were heated and dried, and then powdered with a grinder, charged into a hot water extractor, heated for 10-6000 minutes in a temperature range of 40 to 100 ° C to extract the hot water extract, and then After preparing 10 to 1000 g of a preservative in a weight ratio of 1 liter of hot water extract and stirring for 1 to 5 hours to prepare a composition stock solution, the composition stock solution was diluted in a ratio of 10 to 1000 times with water or beverages. Method of making food. The mushrooms cultivated in the medium containing the selenium purified stock solution were heated and dried, and then powdered with a grinder, charged into a hot water extractor, heated for 10-6000 minutes in a temperature range of 40 to 100 ° C to extract the hot water extract, and then 10 to 1000 g of a preservative was added to 1 L of hot water extract in a weight ratio and stirred for 1 to 5 hours to prepare a composition stock solution, followed by dry powdering of the composition stock solution to form a capsule formulation. . The method for producing a dietary supplement according to claim 1 or 2, wherein the mushroom has a selenium content in the range of 100 to 1000 µg / 100 g. The method of producing a dietary supplement according to claim 1 or 2, wherein the preservative is any one of benzoic acid or sodium benzoate or dehydroacetic acid or sorbic acid or paraoxybenzoic acid esters sodium propionate or calcium salt. The method for producing a dietary supplement according to claim 1 or 2, wherein the mushroom comprises at least one of a top mushroom, a oyster mushroom, a situation mushroom or a mushroom mushroom.