KR102108686B1 - Onion Coat Feed composition Using An Enzyme With Stevia Extract And Method thereof - Google Patents

Abstract

The present invention relates to an onion skin enzyme feed composition comprising a stevia extract comprising an extract of a discarded onion skin and an extract of a natural sugar plant stevia plant as an active ingredient and a method for manufacturing the same.

Description

Onion peel enzyme feed composition containing stevia extract and its manufacturing method {Onion Coat Feed composition Using An Enzyme With Stevia Extract And Method thereof}

The present invention relates to an onion skin enzyme feed composition comprising a stevia extract and a method for manufacturing the same, and more specifically, an onion skin enzyme feed comprising a stevia extract comprising as an active ingredient the discarded onion skin and natural sugar plant stevia. It relates to a composition and a method for manufacturing the same.

Onion (Allium cepa) is a perennial plant of the monocotyledonous Liliaceae family. Food ingredients of onions differ slightly depending on various environmental factors such as varieties, harvest time, soil, and climate, but usually contain 90% moisture, about 8% sugar, and 1.0% protein.

Onion is a vegetable with a lot of protein, and contains glucose, sugar, fructose, and maltose. It has a unique sweet taste. It is also rich in minerals such as potassium, calcium, iron, phosphorus and sodium, dietary fiber, and folic acid. Contains 20mg, low sodium content and no fat.

Onions, in particular, contain a large amount of quercetin, a yellow flavonoid component, which is a bitter taste found in green tea, spinach, broccoli, and parsley. In onions, it is particularly contained in the skin and is a powerful antioxidant, which is a powerful antioxidant that makes the cell membranes of body cells It prevents oxidation of the unsaturated fatty acids that make up and removes free radicals generated in the body, which is great for preventing cancer and aging. Hypertension, hyperlipidemia, diabetes, diet, detoxification, cold, osteoporosis, constipation, insomnia, It is effective in increasing memory, dementia, neuralgia, depression, hair loss, skin beauty, eye diseases, anti-inflammatory, anti-allergic, food poisoning, gastritis, kidney, prostate, eczema, athlete's foot, heavy metal, and yellow dust.

These onions have been used in various foods as vegetables that are effective for health since ancient times. Onions are mainly used as edible stems, and the peculiar smell from scale stems is due to compounds such as propyl disulfide and allyl sulfide. This promotes the secretion of digestive fluids physiologically and has effects such as excitement, sweating, and diuresis.

In addition, the scaled stem contains various vitamins and minerals such as calcium and phosphoric acid, thereby removing harmful substances from the blood. Scale stems are often used in salads, soups, and meat dishes, and as spices in various dishes. The leaves contain 5,000 IU of vitamin A, 45 mg of vitamin C, 80 mg of calcium, 24 mg of magnesium, and 220 mg of potassium in 100 g.

In addition, the onion skin contains about 10 to 100 times higher flavonoid compounds, including quercetin, than the edible portion of onions. In particular, 2.5% by weight or more of the dry peel of onion is quercetin, and because it exists in a free state rather than a glycoside form, it has a stronger bioactive ability than the onion's edible part. However, in general, when processing with onion, the onion peel is removed and only the edible portion of the onion is used. The removed onion peel contains various physiologically active substances including quercetin, but most of them are not applied to products for other purposes. It is a losing situation.

That is, although the excellent components of these onions are generally contained in the most of the onion peel, in reality, most of the onions have a problem of being incinerated by being treated as waste.

Therefore, there is a need for technical improvement of a technique for providing a feed having disease resistance by reusing the onion skin that is discarded to solve the above problems.

Registered Patent Publication No. 10-0775630 (Registration date: 2007.11.05)

The present invention aims to solve the problems of the prior art as described above and the technical problems requested from the past.

The object of the present invention is to include a stevia extract that can improve the disease resistance to foot and mouth disease, bird flu and cholera by supplying animals with feed containing stevia extract, which is a natural sugar plant, as an active ingredient using onion skin that is discarded. To provide an onion peel enzyme feed composition manufacturing method and an enzyme feed prepared thereby.

In addition, another object of the present invention, by providing an enzyme extract containing a natural sugar plant Stevia extract, excellent sweetness efficacy to reduce the spiciness of the original onion includes stevia extract that is easy for children or the public to drink To provide an onion peel enzyme feed composition manufacturing method and an enzyme feed prepared thereby.

Onion peel enzyme feed composition comprising the stevia extract of the present invention for achieving this object may include a mixture of onion skin extract 70 to 95% by weight and stevia extract 30 to 5% by weight as an active ingredient.

In one preferred embodiment of the present invention, the stevia extract may be a steviol, a steviol precursor, or an extract containing at least one of stevioside and / or rebaudioside A. have.

In one preferred example of the present invention, the onion peel enzyme feed composition may be provided in a ring, powder or capsule form, but is not limited thereto.

In one preferred example of the present invention, the extract of the onion peel may be a crude extract prepared by a solvent extraction or hot water extraction method.

In one preferred example of the present invention, the solvent may be purified water, alcohols having 1 to 5 carbons, or organic solvents.

Here, when the solvent is an alcohol, it may be ethanol, methanol, propanol, butanol, ethanol aqueous solution or methanol aqueous solution.

On the other hand, the present invention provides an enzyme feed comprising the onion peel enzyme feed composition.

The present invention also provides a method for preparing an onion peel enzyme feed composition comprising a stevia extract comprising a mixture of 60 to 95% by weight onion peel extract and 40 to 5% by weight stevia extract as an active ingredient.

In one preferred example of the present invention, the extract may be prepared by enzymatic enzymatic processing for 2 to 4 months.

As described above, the onion peel enzyme feed composition comprising the stevia extract according to the present invention uses a waste onion peel to supply animals containing feed containing stevia extract, which is a natural sugar plant, as an active ingredient to foot and mouth disease, algae There is an effect that can improve the disease resistance to the flu and chol.

In addition, by providing an enzyme extract containing an extract of stevia, which is a natural sugar plant, it has an excellent sweetness effect and reduces the spiciness of the original onion, making it easy for children or the public to drink.

1 is a flow chart showing the manufacturing process of an onion peel enzyme feed composition comprising a stevia extract according to an embodiment of the present invention.

The following description of the present invention refers to specific embodiments in which the present invention may be practiced. These examples are described in detail enough to enable those skilled in the art to practice the present invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the technical spirit and scope of the invention in connection with one embodiment.

Therefore, the following detailed description is not intended to be taken in a limiting sense, and the scope of the present invention, if appropriately described, is limited only by the appended claims, along with all ranges equivalent to those claimed.

In addition, although the first and second are used to describe various components in the present invention, it goes without saying that these components are not limited by these terms. These terms are only used to distinguish one component from another component.

Hereinafter, preferred embodiments of the present invention will be described in detail in order to enable those skilled in the art to easily implement the present invention.

As described above, conventionally, there is a problem in that onion peels containing the most excellent components are discarded without reuse to cause environmental pollution.

Accordingly, in the present invention, an onion skin enzyme feed composition comprising a stevia extract comprising a mixture of 60 to 95% by weight onion peel extract and 40 to 5% by weight stevia extract as an active ingredient is prepared and provided as an animal feed. We sought to solve a problem. Through this, by processing the onion skin, which is an environmental pollution, and containing a certain amount, it is possible to provide an onion skin enzyme feed composition including a stevia extract and a method for manufacturing the same.

According to the present invention, the onion peel enzyme feed composition may be prepared as a ring and provided as a feed for livestock, or may be provided in the form of powder or capsul that can be directly consumed by humans.

In the present invention, "feed" is any natural or artificial diet, one meal or the like, or a component of the one meal, for the animal to eat, ingest, and digest, or the mixture of onion skin and stevia or stevioside. Feed containing a feed additive containing as an active ingredient can be prepared in various types of feed known in the art, preferably may include a rich feed, forage and / or special feed.

The term "food product" as used herein refers to any food or feed suitable for consumption by a human or animal.

The term "food supplement" refers to a small amount of compound to supplement a human or animal meal packaged in single or multiple dose units. Food supplements generally do not provide significant amounts of calories, but may contain other micronutrients (eg, vitamins or minerals).

The term "nutritional supplement" means a composition comprising a food supplement along with a calorie source. In some embodiments, the nutritional supplement is a meal replacement or supplement (eg, a nutritional or energy bar or nutritional beverage or concentrate).

As used herein, "prevention" is not intended to mean that a certain event never occurs, but rather means to delay the onset of a certain state or event and reduce the severity of the state or event when the state or event occurs.

First, the onion skin of the present invention is used by crushing itself or using an organic solvent or extracted by water extraction method, preferably purified or treated with ethyl alcohol, water or a mixture or water thereof. Use that. In addition, it is preferable to use fermented alcohol with little harm to the human body as the organic solvent, but any organic pharmaceutically acceptable solvent may be used.

However, in the present invention, an onion peel can be used by including an effective amount in the composition even if it is extracted or produced by any method other than the above method.

According to the present invention, the solvent may be purified water, alcohols having 1 to 5 carbons or organic solvents, and when the solvent is alcohols, it may be ethanol, methanol, propanol, butanol, ethanol aqueous solution or methanol aqueous solution.

According to the present invention, with respect to 100 parts by weight of the whole onion peel enzyme feed composition, the onion peel extract may include 60 to 95% by weight. It may preferably contain 80 to 95% by weight, more preferably 85% by weight. At this time, when the onion peel extract is less than 60% by weight, there may be a problem that pathogenicity is reduced when preparing the feed, and when the onion peel extract exceeds 95% by weight, the sweet taste of the desired feed falls and onions The above range is good because ingestion by intrinsic spicy ingredients may not be easy.

And, in some cases, when prepared in the form of a pharmaceutical preparation, the composition containing the onion peel extract is combined with a pharmaceutically acceptable conventional carrier, depending on the purpose of administration, tablets, hard or soft capsules, chewing tablets It can be formulated in the form of a preparation for oral administration such as a powder, a liquid or a suspension, or a formulation for parenteral administration such as an injectable liquid or a suspension.

At this time, in the case of formulating the onion peel extract of the present invention as a formulation for oral administration such as tablets, capsules, chewing tablets, powders, liquids, suspensions, gum arabic, corn starch, microcrystalline cellulose or gelatin, etc. Binders, excipients such as dicalcium phosphate or lactose, disintegrating agents such as alginic acid, corn starch or potato starch, lubricants such as magnesium stearate, sweeteners such as sucrose or saccharin and peppermint, methyl salicylate or fruit flavors Flavoring agents may be included, and when the dosage unit type is a capsule, a liquid carrier such as polyethylene glycol or fatty oil may be included in addition to the above components.

On the other hand, Stevia is a perennial plant belonging to the family Asteraceae, and is a natural sweetener resource derived from Paraguay. Stevia side sweetness is hundreds of times sweeter than sugar in these stevia plant leaves, and its calories are very low, making it a main ingredient in general foods and dietary supplements. Can write

According to the present invention, the stevia extract may include an extract containing at least one of steviol, a steviol precursor, or stevioside and / or rebaudioside A.

Here, the steviol precursor means a steviol glycoside that can be hydrolyzed by an enzyme to become a free non-saccharide steviol. See, for example, EFSA Journal 2010, 8 (4): 1537, incorporated herein by reference. Enzymes suitable for this hydrolysis are present in certain microorganisms and also in the intestinal tract of humans or animals. See, eg, Gardana et al., 2003, J. Agric. Food Chem. 51: 6618-22.

According to the present invention, with respect to 100 parts by weight of the whole onion peel enzyme feed composition, the stevia extract may include 40 to 5% by weight. Preferably it may contain 30 to 5% by weight, more preferably 15% by weight. At this time, when the onion peel extract is less than 5% by weight, it may be difficult to use the feed because it is difficult to produce a desired sweet taste, and when the stevia extract exceeds 40% by weight, the pathogenicity and efficacy of the feed are reduced. The above range is good because it can be done.

Further, according to the present invention, there is provided an enzyme feed comprising the onion peel enzyme feed composition and fertilizer in a content ratio of 3: 7 to 2: 8. Here, when the onion peel enzyme feed composition and fertilizer are out of the range of 2 to 3 content ratio, the above range is good because the feed containing the desired antibacterial effect cannot be prepared.

Hereinafter, a method of preparing an onion peel enzyme feed composition comprising a stevia extract according to the present invention will be described.

The present invention provides a method for preparing an onion peel enzyme feed composition comprising a stevia extract comprising a mixture of 60 to 95% by weight onion peel extract and 40 to 5% by weight stevia extract as an active ingredient.

1 schematically shows a flow chart of a method for preparing an onion peel enzyme feed composition comprising a stevia extract according to one embodiment of the present invention.

Specifically, as shown in Figure 1, the onion peel enzyme feed composition comprising the stevia extract according to the present invention, the process of collecting stevia plants and washing them with drinking water (S1), cutting and crushing the washed stevia plants The process (S2), the process of collecting the onion skin and washing it with drinking water to crush it (S3), the process of mixing the stevia plant and the crushed onion skin to prepare a mixture (S4), the stevia plant and the crushed onion The shell mixture is placed in an extractor and heated to 80 to 150 degrees, and the mixture is heated at room temperature to cool slowly (S5) and the mixture is aged at 2 to 4 months at 55 to 75 degrees (S6).

First, stevia extract and onion skin extract are prepared through the washing and grinding process using the stevia (S1 to S3).

According to the present invention, the onion peel can be used by grinding, and, if necessary, can be prepared as an extract through a separate extraction process.

When describing the onion peel extraction process, the onion peel extract may be prepared using a hot water extraction method.

Specifically, the onion is washed by using the water pressure of drinking water to remove foreign substances such as remaining soil and microorganisms, and onion peels separated by water pressure are collected during the washing process to remove parts other than onion roots such as onion roots and good quality. Secure the onion peel of the. The selected onion skin is dried in a cow, and the dried onion skin is pulverized using a pin crusher or a catalytic dry grinding machine to prepare onion skin powder. Depending on the design conditions, the onion peel can be pulverized using a multi-purpose grinder, a dry grinder of various methods such as hammer mill, and the like.

The pulverized onion skin powder is then made to a concentration of 10 to 20 mg / ml using purified water, followed by hot water extraction (heat extraction) at 80 to 100 ° C and pH 6 to 7 for 30 to 60 minutes.

Preferably, the concentration of the onion skin powder is 20 mg / ml, the temperature is 100 ° C., and the pH is adjusted to pH 6 using a phosphate buffer solution to extract hot water for 30 minutes. This is a hot water extraction condition to obtain an extract containing a high content of physiologically active substances from onion skin powder.

In addition, according to the present invention, the stevia plant may be pulverized or mixed with the extracted onion peel, or may be separately prepared through an extraction process.

Explaining the stevia extraction process, stevia leaves and stems may be used as a stevia plant tissue, and extracts may be extracted using only stevia leaves or extracts using only stevia stems, depending on the usage. The extract may be extracted by mixing a proper amount of fruit and stem.

In the extraction treatment of plant tissues such as stevia leaves and stems, the plant tissue is dried and crushed, and the crushed crushed material is placed in an extraction container containing water and heated to extract the extract from the plant tissue of Stevia. In order to extract the extract from the pulverized product more effectively, other solvents may be mixed with water, and the extract may be extracted from the crushed product under a pressure above atmospheric pressure.

In the case of extracting the extract from the crushed product under a pressure above atmospheric pressure, the boiling point of water, etc., in which the pulverized product is contained increases, thereby effectively extracting the active ingredient contained in the stevia plant tissue.

Specifically, first, a stevia leaf having a rebaudioside content of 10% by weight or more is harvested on the stevia leaf, and then foreign substances such as attached dust are washed with water and left for 3 hours in a well-ventilated shade to discolor the leaf to green. To dry. When selecting and using stevia leaves having a rebaudioside content of 10% by weight or more on the stevia leaf, the bitter taste is less, and the rebaudioside can improve the palatability by reducing the peculiar strong sweetness of stevioside.

Here, the process of cutting and drying the stevia leaf is repeated until the moisture content is 45 to 55% by weight. In some cases, it is desirable to rub the dried stevia leaves with a slicing machine to destroy the cell walls of the stevia leaves.

At this time, the moisture content of the stevia leaf is important, and since it is used only with the onion peel, there is little moisture, so the moisture of the stevia leaf can be appropriately used. When the moisture content of the stevia leaf is less than 45% by weight, fermentation may not be smooth, and when the moisture content exceeds 55% by weight, harmful molds may multiply during fermentation, so the above range is good.

Next, a process of mixing the stevia extract and the onion peel extract prepared as described above is performed (S4). As described above, the mixture made of the stevia extract and the onion peel extract is placed in an extractor and heated before mixing, by mixing the mixture in a high-speed mixer.

Next, a process of heating and drying the mixture made of the onion peel extract and stevia extract is performed (S5). It may be repeatedly performed for 5 minutes to 30 minutes at approximately 8-150 degrees.

Through the process of heating the mixture, stevioside and moisture in the stevia plant are extracted in addition to the extract in the onion skin and moisture in the onion skin by the pressure and high temperature of the hot steam to prepare an extract containing sweetness.

Next, if necessary, a fermentation process is performed. The concentrate is fermented at a temperature of 20 to 30 degrees. At this time, it is preferable to inject oxygen into the concentrate at regular time intervals. When the yeast bacteria involved in fermentation are mainly aerobic microorganisms and oxygen is periodically injected into the concentrate, the fermentation and growth of the yeast bacteria involved in fermentation occur vigorously, further promoting fermentation, as well as creating a growth environment in which anaerobic microorganisms such as decaying bacteria are difficult to develop. By providing, there is an advantage of preventing fermentation caused by spoilage bacteria during fermentation.

Finally, the aging process proceeds, and this aging process may be performed at approximately 55 to 75 degrees for approximately 2 to 4 months to prepare an onion peel enzyme feed composition comprising a stevia extract.

Here, according to the present invention, preferably, the aging process (S7) can be aged for 2.5 to 3.5 months at 60 degrees to 70 degrees, and more preferably for 3 months at 65 degrees.

Hereinafter, preferred examples are provided to help understanding of the present invention. However, the following examples are only to aid the understanding of the present invention, and the present invention is not limited by the following experimental examples.

[Production example]

<Production Example 1> Onion peel extract

Onion peel (Fomitopsis pinicola (Sw.ex Fr.) Karst.) Is chopped to about 23cm, and 2l of 60% (v / v) ethyl alcohol per 100g of dry weight is added and extracted at 60 ℃, then sieve (0.054mm) and filter paper ( The extract was obtained by filtration using Whatman No 2).

<Production Example 2> Stevia plant extract

Stevia leaves and stems were dried, crushed and mixed, and then 3 kg each was placed in a water-resistant heat-resistant bag. Into an extraction container containing water, 2 heat-resistant bags containing crushed materials were added, and heated to a temperature of 110 ± 10 ° C for 2.5 hours to extract the active ingredient contained in stevia. After the extracted extract was placed in a concentration tank, the mixture was heated and stirred at a temperature of 110 ± 10 ° C. under negative pressure for 13 to 15 hours to obtain a concentrated solution. Then, the concentrate is placed in a fermentation container, and then, after treating with granular activated carbon, the fermentation process is performed for 5 days at 20 to 30 ° C, and then matured for approximately 150 days at a state of 20 to 30 ° C to complete stevia extract having a suitable antioxidant index. Did.

[Example] Preparation of onion peel enzyme feed composition and enzyme feed containing stevia extract

<Example 1>

85% by weight of the onion peel extract and 15% by weight of stevia extract prepared in Preparation Example 1 and Preparation Example 2 were blended into aquaculture feed, added to an extractor, heated at a temperature of 120 ° C for 2.5 hours, and stirred at the same time to concentrate the concentrate. Got. Then, the concentrate was placed in a fermentation container, and then subjected to a fermentation process at 25 ° C for 5 days in a state where granular activated carbon was treated, and then aged in an environment of approximately 65 ° C for approximately 90 days to prepare feed in a ring.

<Example 2>

A ring-like feed was prepared in the same manner as in Example 1, except that the onion skin extract was mixed at 60% by weight and stevia extract at 40% by weight.

[Comparative example]

<Comparative Example 1>

A pill-like feed was prepared in the same manner as in Example 1, except that fructose was used as the sugar instead of the stevia extract.

<Comparative Example 2>

A ring-like feed was prepared in the same manner as in Example 1, except that granular activated carbon was not treated.

[Experimental Example]

<Experimental Example 1>

The fermentation period of the concentrates of Examples 1 to 2 and Comparative Examples 1 to 2 was compared with the fermentation period, the degree of decay, the sensory test of the concentrate during fermentation, and the maturation time to obtain an extract having a suitable antioxidant index. It is shown in. At this time, in the case of corruption in the fermentation process, the occurrence of decay was visually indicated as '○', and when it was confirmed only partially, it was denoted as '△', and if not confirmed, it was denoted as '×'. The test was evaluated for 5 males and 5 females by the 9-point scoring method (9-very good, 7-good, 5-normal, 3-bad, 1-very bad) for the preference of odor.

Fermentation period Corruption Odor during fermentation Ripening period Example 1 5 × 7.50 90 Example 2 5 × 6.40 90 Comparative Example 1 5 × 3.40 120 Comparative Example 2 9 O 2.70 150

As can be seen in Table 1, when the granular activated carbon is treated in the concentrate during the fermentation process as in Examples 1 and 2, the stench caused by decay, etc., and the peculiar smell generated during fermentation are compared to Comparative Example 2 in which the activated carbon is not treated. Compared to a relatively small amount, it was confirmed that the fermentation period and aging period were significantly reduced. In addition, Comparative Example 1, which used activated carbon but treated with fructose instead of stevia, had a relatively slight increase in odor during fermentation and slightly increased maturation period compared to Example 1.

<Experimental Example 2>

Sensory evaluations were performed on the sweetness, color, aroma, odor, and overall preference for the enzyme feeds of Examples 1 to 2 and Comparative Examples 1 to 2. At this time, for the concentrate prepared by mixing the onion peel extract and the stevia extract, nine points were scored for 20 adult men and women (9-very good, 7-good, 5-normal, 3-bad, 1- Sensory evaluation), and the results are shown in Table 2.

sweetness color incense smell Preference Example 1 8.3 8.2 8.3 8.1 8.2 Example 2 9.5 8.9 8.6 8.5 8.6 Comparative Example 1 2.1 7.5 7.3 7.1 6.7 Comparative Example 2 8.3 8.9 6.1 5.4 7.2

As can be seen in Table 1, Examples 1 to 2 and Comparative Example 2, which contain stevia in a predetermined content ratio, are compared with Comparative Example 1 in which the preference for sweetness, color, and odor, etc., includes fructose instead of stevia. In contrast, it was improved. However, in the case of Comparative Example 2, which was not treated with activated carbon, it was found that the overall preference was slightly decreased due to an increase in aroma and odor.

Although described above with reference to the drawings according to embodiments of the present invention, those skilled in the art to which the present invention pertains will be able to perform various applications and modifications within the scope of the present invention based on the above.

Claims (10)

delete delete delete delete delete delete delete delete In the method for preparing an onion peel enzyme feed composition comprising a stevia extract using as a mixture a mixture of 60 to 85% by weight of discarded onion peel extract and 40 to 15% by weight of stevia extract,
The stevia extract is an extract containing at least one of steviol and a steviol precursor,
When the onion peel extract is formulated as an oral administration preparation for tablets, capsules, chewing tablets, powders, liquids or suspensions, a binder selected from gum arabic, corn starch, microcrystalline cellulose or gelatin, dicalcium phosphate, or Contains excipients selected from lactose, disintegrants selected from alginic acid, corn starch or potato starch, magnesium stearate lubricants, sweeteners selected from sucrose or saccharin and flavoring agents selected from peppermint, methyl salicylate or fruit flavors And
The onion skin extract is solvent-extracted with a solvent selected from ethanol, methanol, propanol, butanol, ethanol aqueous solution or methanol aqueous solution at 60 ° C,
Method for preparing an onion peel enzyme feed composition comprising a stevia extract, characterized in that the stevia extract and onion skin extract are enzymatically processed for 2 to 4 months through a fermentation process in the state of granular activated carbon treatment. .
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