RU2452500C1 - Method for preparing pharmacologically active concentrate of tambukan therapeutic mud - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, namely a method for preparing a concentrate of Tambukan therapeutic mud. The method for preparing the concentrate of Tambukan therapeutic mud consisting in the fact that mud is pressed, filled with mixed ethanol and oil in the presence of Tween 80; the extract is poured out; mud is pressed and secondary filled with mixed ethanol and oil with added emulsifier T-2; the combined extracts are sterilised under certain conditions.

EFFECT: method enables increasing yield of hydrophobic substances, intensity of extraction process and reducing extraction time.

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Description

The invention relates to the field of pharmaceutical industry, namely to the production of extracts from therapeutic natural mud, and can be used in the pharmaceutical industry and medicine.

The following methods are known from the prior art for producing extracts from therapeutic muds.

The method of obtaining the product from the Tambukan therapeutic mud (Patent RU No. 2313351), including dehydration of the raw materials, grinding, oil-alcohol extraction, dissolving the raw materials in ethyl alcohol in a ratio of 1: 1, the resulting mixture is loaded into the extractor and stirred under heating under a vacuum of 0.4 -0.5 atm, then the mixture is brought to a boil and left to stand, the resulting alcoholic extract is drained and filtered, after which vegetable oil is added to the mass remaining in the extractor containing therapeutic mud, in the ratio mass: oil = 1: 0.5, obtained the th mixture is stirred in an extractor under a vacuum of 0.6 atm with simultaneous distillation of the alcohol residues until complete removal, the resulting product is defended and cooled.

Sterilization of dirt by pressure relief is not achieved, since pressure relief from 66 atmospheres to atmospheric, regardless of the speed of discharge, does not lead to rupture and death (provisioning) of microorganisms, and even more so spores can break apart only macrocells (for example, algae residues and hydrobiont cells ) and other dirt macrostructures.

A method of obtaining a therapeutic extract, including dehydration of the therapeutic silt mud of mainland lakes, its grinding, extraction with a mixture of ethanol, distillation of the solvent, dissolution of the extract in liquid paraffin and sterilization, use native mud, dehydration is carried out by pressing to a moisture content of 25-27%. The squeezed dirt is crushed to the maximum extent possible; before extraction, the crushed dirt is dissolved in 96% ethanol in a 1: 1 ratio with heating to 35–40 ° C and stirring, sterilized in the extractor by supplying gaseous carbon dioxide under a pressure of 66 atm, and kept under this pressure for 15 minutes, followed by a sharp depressurization to atmospheric pressure at a rate of 5 atm / s, and extraction is carried out by feeding liquid carbon dioxide to the extractor in a ratio of 2: 5 with vigorous stirring for 60 minutes, alcohol-ug the acidic miscella is left to stand for 65 minutes, and the solvent is distilled off in an evaporator at the same time as the extract is dissolved in oil by evaporation of carbon dioxide, and then under reduced pressure - alcohol (Patent RU No. 2066187). The disadvantages include sterilization under pressure, which leads to the death of beneficial microorganisms, the mud of Lake Tambukan is itself almost sterile due to the activity of saprophytic microorganisms that live in the mud and are antagonists of pathogenic microorganisms.

A known method of obtaining a medicinal product from therapeutic mud, including dehydration, extraction and distillation of ethanol in the presence of oil, characterized in that the Tambukan native mud with a natural humidity of 50-60% is dehydrated by pressing under a load to a residual moisture content of 30-36%, extracted with oil and ethanol in a mass ratio of 1.5: 0.5: 1.0, respectively, at 98-100 ° C to a concentration of the sum of carotenoids in a separate sample of at least 22 mg%, the resulting concentrate is diluted with sterile oil in a ratio of 1: 9 and sterilized for 2- 3 hours at 125 -130 ° C (Patent RU No. 2145225).

Also known is a method (Patent RU 2177800), which consists in obtaining a therapeutic extract from a native therapeutic mud (Tambukan mud), which is dehydrated under load to a moisture content of 25%. The pressed mud is crushed. Oil and alcohol are added to the crushed mud at a ratio of 1: 0.2 and 1: 0.3 when heated. The extraction is carried out twice in succession. Moreover, the first extraction is carried out under a vacuum of 0.75-0.85 atm, and the second extraction is carried out in two stages at atmospheric pressure, then under vacuum. The resulting portions of extracts are poured into a container and mixed.

The disadvantage of this method is the duration of the extraction process. This method is taken as a prototype.

The technical task of the invention is to increase the yield of hydrophobic active substances, increase the biological activity of the target product, increase the intensity of the extraction process and reduce the time of extraction.

The stated technical problem is achieved by the method of obtaining in two stages, which are carried out sequentially, the first stage is as follows, the native sludge therapeutic mud is squeezed to a residual moisture content of 30-36%, filled with a mixture of alcohol 76% and oil, maintaining the ratio of mud-alcohol-oil ( 3: 1: 2) in the presence of tween-80 in an amount of 1%, extraction is carried out at a temperature of 60 ° C for 50 minutes, the extract is drained, the dirt is squeezed out. The mud is poured a second time, it is poured with a mixture of alcohol 76% and oil, maintaining the dirt-alcohol-oil ratio (3: 1: 2) with the addition of T-2 emulsifier in an amount of 2% for 30 min at a temperature of 60 °. The extracts are combined and sterilized for one hour at a temperature of 100 ° C.

An example of a specific implementation No. 1

Mud with a natural moisture content of 60 kg is loaded into the reactor, dehydrated to a moisture content of 30%, 20 kg of ethanol and 40 kg of oil are added, i.e. maintain a ratio of 3: 1: 2 with the addition of 0.6 kg of Tween-80 and extract the lipid complex at a temperature of 60 ° C for 30 minutes Then the extract is drained, the dirt is squeezed out and poured a second time with a mixture of 20 kg of alcohol and 40 kg of oil with the addition of T-2 emulsifier in an amount of 1.2 kg and extracted at a temperature of 60 ° C for 30 minutes. The extract is drained, the dirt is squeezed out. The extracts are combined and sterilized.

An example of a specific implementation No. 2

Mud with natural humidity and a mass of 15 kg is loaded into the reactor, dehydrated to a moisture content of 35%, 5 kg of ethanol and 10 kg of corn oil are added, i.e. maintain a ratio of 3: 1: 2 with the addition of 0.15 kg (150 g) of Tween-80 and extract the lipid complex at a temperature of 60 ° C for 30 minutes Then the extract is drained, the dirt is squeezed out and poured a second time with a mixture of alcohol 5 kg and corn oil 10 kg 0.3 kg emulsifier T-2 and extracted at a temperature of 60 ° C for 30 minutes. The extract is drained, the dirt is squeezed out. The extracts are combined and sterilized.

An example of a specific implementation No. 3

Mud with natural moisture and a mass of 12 kg is loaded into the reactor, dehydrated to a moisture content of 36%, 4 kg of ethanol and 8 kg of corn oil are added, i.e. maintain a ratio of 3: 1: 2 with the addition of 0.12 kg (120 g) of Tween-80 and extract the lipid complex at a temperature of 60 ° C for 30 minutes Then the extract is drained, the dirt is squeezed out and again poured with a mixture of alcohol 4 kg and olive oil 8 kg with the addition of T-2 emulsifier in an amount of 0.24 kg (240 g) and extracted at a temperature of 60 ° C for 30 minutes. The extract is drained, the dirt is squeezed out. The extracts are combined and sterilized.

We have selected the optimal amount of tween - 80 and emulsifier, increasing or decreasing their content leads to a decrease in the yield of active substances.

As - oils, in the first place, can be taken: olive, corn and sunflower oils, liquid paraffin, cocoa butter (more preferably olive oil).

Tests for sterility and microbiological frequency were carried out according to the requirements of the Global Fund (State Pharmacopoeia of the USSR, 11. Issue 2. General methods of analysis. - M .: Medicine, 1990, p. 187 and 193). Studies have shown the necessary pharmacopoeial level of sterility and microbiological purity.

Studies on the quantitative content of carotenoids in the concentrate depending on humidity showed that the proposed method allows to increase the number of active substances with a moisture content of 30-40%. Table N 1 presents data on the dependence of the yield of the target product on the moisture content in silt mud for extraction.

From the table it follows that the optimal yield of the sum of carotenoids is observed when the moisture content in the mud is 30-36% and is 24.7 mg%, and exceeds the results of the output of the prototype.

Thus, the proposed method for producing concentrate from Tambukan mud allows to increase the yield of carotenoids, increase the intensity of the extraction process and reduce the time required to reduce the moisture content of the raw materials and the extraction time.

Table number 1 The content of carotenoids in the concentrate from Tambukan mud depending on the moisture content. No. The moisture content in the mud% The output of the amount of carotenoids (mg%) one 24 22.3 2 28 22.4 3 thirty 24.7 four 32 24.7 5 34 24.7 6 36 24.7 7 38 22.1 8 40 22.1

Claims (1)

A method of obtaining a concentrate from the Tambukan therapeutic mud by extracting dehydrated mud with a mixture of alcohol and oil in two stages by heating, combining the extracts, characterized in that the mud is squeezed to a residual moisture content of 30-40%, poured with a mixture of ethyl alcohol 76% and oil with a ratio of dirt: alcohol: oil (3: 1: 2) in the presence of tween-80 in an amount of 1% by weight of the raw material, extraction is carried out at a temperature of 60 ° C for 50 minutes, the extract is drained, the dirt is squeezed out and poured a second time with a mixture of ethyl alcohol 76% and oil at a ratio of dirt: alcohol: wt Lo (3: 1: 2) with the addition of T-2 emulsifier in an amount of 2% by weight of the raw material for 30 min at a temperature of 60 ° C, the combined extracts are sterilized for one hour at a temperature of 100 ° C.