RU2177800C2 - Method of preparing curative extract from vegetable or peloid raw - Google Patents

Abstract

FIELD: medicine, pharmaceutical technology. SUBSTANCE: vegetable or peloid raw is milled and dehydrated to the loss of fluidity, stirred preliminary with oil and alcohol at heating in the ratio 1 kg : 0.2 l and 1 kg : 0,3 l, respectively. Raw is extracted with oil in the presence of boiling alcohol and its distillate is dehydrated under vacuum to moisture value 0.1%, not above. Raw extraction is carried out in the ratio from 1 kg of raw : 0.3 l to 1 kg of raw : 1 l of oil and from 1 kg : 0.4 l to 1 kg : 1 l of alcohol twice successively with the same portion of raw and at the same ratio of raw to oil and alcohol in both extraction stages. The first extraction is carried out under vacuum 0.75-0.85 atm, at temperature 50 C, not above, and the second extraction is carried out for two stages: under atmosphere pressure and temperature 85 C, not above, and then under vacuum 0.87-0.85 atm. Obtained portions of extracts are poured into total capacity and stirred. EFFECT: intensified process, increased yield and biological activity of product.

Description

 The invention relates to the medical industry and relates to a technology for the production of biologically active substances from natural raw materials. Known methods for producing oil extracts from natural raw materials, for example, "Method for producing extract of therapeutic mud" (Russian patent 2073999, Tolcheev A.V. Bul. 6 from 02.27.97, priority from 09.27.93).

 The disadvantage of this method is the incomplete extraction of useful substances, in connection with oxidative processes that occur during extraction in alcohol, as well as with subsequent distillation of alcohol with the addition of liquid paraffin. For example, the content of β-carotene is reduced three times.

 The closest in technical essence to the claimed method and its prototype is "A method of obtaining a medicinal extract from plant or peloid raw materials" (Russian patent 2126686, Scherbak I.F., Tolcheev A.V. Bul. 6 from 02.27.99, priority March 26, 1997).

In this method, the raw materials are dehydrated to yield strength and then extracted with oil at a temperature of up to 85 ^{° C.} , providing boiling of the alcohol and its distillation. The finished extract is dehydrated under vacuum to a moisture content of not more than 0.1%. In this case, before extraction, the raw materials are mixed first with oil in a ratio of 1 kg: 0.2 l, then with alcohol in a ratio of 1 kg: 0.3 l.

 The method allows to intensify the extraction process, to increase the extraction and biological activity of the resulting oil extract.

The disadvantage of this method is the lack of intensity and long duration of the extraction process, since it occurs at atmospheric pressure. In connection with the extraction process at a temperature of distillation of alcohol to 85 ^o With, part of the thermolabile substances is destroyed, which reduces the biological activity of the final product.

The claimed technical solution provides the following results:
intensification of the extraction process and reduction of time by more than 2 times;
increased extraction of the target product, in particular thermolabile substances;
increasing the biological activity of the target product.

This is achieved by the following set of essential features:
extraction of raw materials is carried out at a ratio of 1 kg of raw materials: 0.3 l of oil to 1 kg of raw materials: 1 l of oil and from 1 kg of raw materials: 0.4 l of alcohol to 1 kg: 1 l of alcohol;
carry out not one, but two sequential extraction of a given portion of the feed with oil and alcohol in both extractions;
the first extraction is carried out completely under a vacuum of 0.75-0.85 atm. at a temperature of up to 50 ^o C, after distilling off the alcohol and draining the extract, pour in a portion of the solvents (oil and alcohol) for the second extraction;
the first stage of the second extraction is carried out at atmospheric pressure at a temperature of up to 85 ^o C, which ensures the selection in the extract of substances requiring a higher temperature for complete extraction;
the second stage of the second extraction is carried out under a vacuum of 0.75-0.85 atm. to accelerate the distillation of alcohol;
the extracts obtained during the first and second extraction are poured into a common container, which gives the final product with maximum biological activity.

As can be seen from the comparison with the prototype, in the inventive method, the novelty is as follows:
carry out two extractions of the same portion of raw materials, instead of one extraction;
the first extraction is completely carried out under a vacuum of 0.75-0.85 atm. and at temperatures up to 50 ^o C, instead of carrying out extraction at atmospheric pressure, and a temperature up to 85 ^o C;
apply the ratio of raw materials and oil in the range from 1 kg: 0.3 l to 1 kg: 1 l, instead of 1 kg: 0.2 l;
use a greater amount of alcohol with an exposure ratio of raw materials and alcohol in the range from 1 kg: 0.4 l to 1 kg: 1 l, instead of 1 kg: 0.3 l.

The relationship of these new significant features with the achieved technical results was established by the author in the process of experimental work and consists in the following:
application for oil extraction within the ratio of raw materials and oil from 1 kg: 0.3 l to 1 kg: 1 l allows you to get the final product of the required concentration, corresponding to the current documentation;
the use of a larger amount of alcohol (up to a ratio of 1 l: 1 kg) ensures greater fluidity of the extracted raw material and its maximum contact with solvents, which accelerates the extraction process and its completeness;
application of vacuum 0.75-0.85 atm. provides extraction at a temperature not exceeding 50 ^o C, which preserves thermolabile substances and increases the biological activity of the extract;
the use of the second extraction at atmospheric pressure provides a temperature of up to 85 ^o C and the release of substances that require a higher temperature for complete extraction;
application for vacuum extraction accelerates the process of distillation of alcohol and reduces the total time for two extractions of the same portion of raw materials more than double compared to the prototype;
the combination of extracts of the first and second extraction gives the target product of high biological activity and higher concentration.

 Confirmation of the possibility of carrying out the invention is proved by the following examples of experimental work on the development of therapeutic oil extracts from two types of raw materials.

 Example 1

The crushed pulp of sea buckthorn fruits of pear-shaped mass of 10.0 kg was dehydrated to a moisture content of 25-27%, then, when heated to 45 ^o С, 2 l of refined sunflower oil was added to the mass of raw materials (with constant stirring of the mass), then 3 l of alcohol was added (stirring continued )

Another 6 liters of sunflower oil and 3 liters of alcohol are poured into the resulting homogeneous mass. The vacuum system and extractor heating are turned on. Vacuum extraction was performed at about 0.8 atm. and at a temperature of 45-47 ^o With distillation of alcohol.

A liquid fraction of about 6 liters was drained. Re-filled 6 liters of sunflower oil and 6 liters of alcohol. At atmospheric pressure, extraction was carried out at a temperature of 79 ^o C. After distillation of 3 l of alcohol, the vacuum system was turned on and at a vacuum of 0.8 atm. distilled alcohol. After settling and squeezing, the liquid fraction in the amount of 6.8 liters is drained. The total amount of extract obtained was 12.8 liters. The resulting product was monitored for the content of the basic substance of carotenoids, which amounted to 185 mg% (according to TU not less than 180 mg%). Comparison of the yield of a useful product from a similar amount of raw material obtained by the method of the prototype showed an increase in yield of 2 l when calculating the same concentration.

 Example 2

Peloids (native healing mud) of Lake Tambukan, which were crushed by the algae contained in them during pumping, were dehydrated to a moisture content of about 25%, and taken in a mass of 10 kg, where 2 l of corn oil and 3 l were added while heating to 45 ^° C and mixed l of alcohol.

Another 4 liters of corn oil and 7 liters of alcohol are poured into the resulting homogeneous mass. The vacuum system and extractor heating are turned on. After that, extraction was carried out with periodic stirring under vacuum at about 0.8 atm. and a temperature of 48-50 ^o With simultaneous distillation of alcohol. Upon completion of the extraction, the oil extract is drained in an amount of 4 l. Then 4 l of corn oil and 10 l of alcohol are re-poured and re-extraction is carried out at atmospheric pressure and a temperature of 83 ^o C. After distillation of 5 l of alcohol, the vacuum system is turned on and at a vacuum of about 0.8 atm. the final distillation of alcohol is made.

 After settling and squeezing, 5 l of oil extract is drained. The total amount of extract obtained was 9 l. The resulting preparation was tested for carotenoids, the concentration of which was 12.9 mg% (in accordance with the requirements of TU 9369-001-32629188-98 should be at least 12 mg%).

 Comparison of the yield of a useful product from an equal amount of raw materials in the proposed method and the prototype method showed an increase in yield in the proposed method by 1.5 liters (in terms of an equal concentration of carotenoids), while reducing time costs by 2.1 times.

 As a source of raw materials in the inventive method can be used crushed mud silt mud of continental lakes, as well as plant raw materials, crushed to a pasty state (various herbs, their flowers, roots and rhizomes, fruits and berries, vegetables, algae and animal raw materials).

 As a solvent, instead of corn oil, various vegetable oils can be used: olive, sunflower, cocoa butter or its substitutes. The use of synthetic oils, including petroleum jelly, is not advisable, due to their poor absorption and worse extraction of nutrients than vegetable oils.

Claims (1)

A method of obtaining a medicinal extract with anti-inflammatory action from crushed and dehydrated to yield fluid plant or native peloid raw materials, including pre-mixing it when heated with oil and alcohol in the ratios of 1 kg: 0.2 l and 1 kg: 0.3 l, respectively extraction of raw materials in oil in the presence of alcohol, ensuring the boiling of alcohol and its distillation, followed by dehydration of the extract under vacuum to a moisture content of not more than 0.1%, characterized in that the extraction of raw materials is carried out at a ratio from 1 kg of raw materials: 0.3 l to 1 kg of raw materials: 1 l of oil and from 1 kg: 0.4 l to 1 kg: 1 l of alcohol, two times in succession using the same portion of raw materials, while maintaining the same ratio raw materials with oil and alcohol in both extractions, the first extraction being carried out under vacuum of 0.75-0.85 atm at temperatures up to 50 ^o С, the second extraction is carried out in two stages: at atmospheric pressure and temperature up to 85 ^o С, then under vacuum 0.75-0.85 atm, the resulting portion of the extracts is poured into a collecting tank and mixed.