RU2409347C1 - Agent to correct stress conditions in animals - Google Patents

Abstract

FIELD: medicine, veterinary science. ^ SUBSTANCE: invention relates to veterinary science, namely to stress correctors and adaptogens for animals. Preparation for correction contains selenium in nanosize condition and zero valency (Se0) , ascorbic acid and water for injections, at the following ratio of components in wt %: selenium (Se0) 0.001-1.0; ascorbic acid 5.0-20.0; water for injections - balance. ^ EFFECT: invention provides for reduction of toxicity, high availability for organism, higher antioxidant and anti-stress effect of agent, lower costs for its production. ^ 2 tbl, 4 ex

Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to stress correctors and adaptogens for animals.

State of the art

Known complex oil multivitamin preparation that regulates metabolic processes and reduces the frequency of selenium deficiency in animals, including birds, containing selenium and an oil solvent, while containing selenium in the form of sodium selenite, retinol acetate as vitamin A, and the oil solvent is corn germ oil, and the components are taken in the following proportions:

Retinol acetate (250 thousand IU / ml) 60 ml

Sodium Selenite 1 g

Corn germ oil up to 1000 ml

(See Pat. RU No. 2250099, Cl. A61K 9/08, A61K 33/04, A61K 31/07, A61K 35/78, published on 04/20/2005)

The disadvantage of this drug is that sodium selenite in its composition is a more toxic compound than the proposed selenium (Se ⁰ ).

A known drug is a suspension of barium selenate and selenite in liquid paraffin, its administration to sows on the 32nd day of gestation increases the content of selenium in blood and milk, prevents excessive accumulation of toxic lipid peroxidation products in the body, normalizes metabolism, reduces the stillbirth of piglets and postpartum morbidity uterus (See VN Kotsarev, VD Misailov, BC Buzlama Depolen for the prevention of metritis-mastitis-agalactia in sows // Veterinary medicine. - 2005. - No. 1. P.39-42).

The disadvantage of this drug is the lack of anti-stress effect and the complexity of the introduction.

A known preparation containing a water-dispersed form of vitamins A, D ₃ , E and C. The use of this drug helps to reduce the manifestation of uterine subinvolution, retention of the placenta, postpartum endometritis and more fruitful insemination of cows (See V.A. Sidorkin, V.A. Koptsev, A.N. Turchenko, The effect of water-dispersed forms of vitamins on the reproductive system of cows // Veterinary Medicine. - 2003. - No. 9. - P.33-34).

The disadvantage of this drug is the lack of antioxidant and anti-stress effect.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is the drug, which is an aqueous solution containing 25 ml of tocopherol acetate in 1 ml and 2.2 mg of sodium selenite with the addition of auxiliary substances. Its introduction increases the concentration of selenium in the blood of calves, helps to reduce the formation of lipid peroxidation products and has a positive effect on the gain in live weight (See V.A. Belyaev, V.A. Orobets, I.V. Kireev. Effect of selemagus on the productivity of young large cattle // Actual issues of zootechnical science and practice as the basis for improving the productive qualities and health of farm animals: International Scientific and Practical Conf. / Collected Scientific Trucks - Stavropol: Agrus, 2007. - P.249-252) .

The disadvantage of this drug is a less pronounced anti-stress effect, insufficient antioxidant effect and relatively high toxicity.

Disclosure of invention

The task of the invention is to develop a drug with anti-stress effect and adaptogenic effect.

The technical result that can be achieved using the present invention is reduced to anti-stress action, antioxidant effect and lower manufacturing costs.

The technical result is achieved using a preparation for the correction of stress conditions in animals, including the inorganic form of selenium, water for injection, while it is additionally provided with ascorbic acid, and selenium in the nanoscale state and zero valency (Se ⁰ ) is used as the inorganic form of selenium in the following the ratio of components in wt.%:

selenium (Se ⁰ ) 0.001-1.0 ascorbic acid 5.0-20.0 water for injections rest

The essence of obtaining a drug for the correction of stress conditions in animals is as follows: a stabilizer polyvinylpyrrolidone is added to an aqueous solution of H ₂ Se0 ₃ , mixed under aseptic conditions with an aqueous solution of ascorbic acid taken in 5-10% excess relative to equivalent, an aqueous solution containing selenium is obtained (Se ⁰ ) and ascorbic acid in the following ratio of components in wt.%:

selenium (Se ⁰ ) 0.001-1.0 ascorbic acid 5.0-20.0 water for injections rest

The pharmacological effect of the drug is that drugs with adaptogenic and antioxidant effects can regulate the level of free radical processes and, through them, affect both oxidative metabolism and the development of immune responses. Among these substances, selenium compounds are the most promising (See I.A. Lykasova, S.P. Merenkova. Effect of feed additives nutril selenium on the state of lipid peroxidation in pigs under intensive feeding // Free radicals, antioxidants, health animals: Materials of the international scientific-practical conference. - Voronezh, 2004. - P.235). It is known that selenium is an essential microelement for living organisms that are part of the most important enzymes involved in the oxidative processes of animals and humans. Selenium is part of the antioxidant enzyme glutathione peroxidase, which protects the body from the damaging effects of lipid peroxidation products, and has a regulatory effect on other metabolic processes in the body (see V.A. Antipov, T.N. Rodionova, and T.S. Gerashchenko // Free radicals, antioxidants, animal health: Materials of the international scientific-practical conference. - Voronezh, 2004. - P.159-169). Selenium preparations eliminate stress, promote antioxidant protection (See K.P.Surai, PFSurai, B.K.Speake et al. Antioxidant prooxidant balans in the intestine // Food for thought. - 2003 - V.1. - No. 1.- P.51-70). The intensity of free radical processes in the body and a decrease in the level of immune reactions are amplified against the background of insufficient intake of the trace element selenium. Thus, currently, selenium preparations are widely used to reduce the excessive activation of lipid peroxidation processes and stimulate antioxidant protection in veterinary medicine and medicine (See A.F. Blinohvatov. Selenium in the biosphere. - Penza, 2001. - P.154-180).

Ascorbic acid supplements improve immune responses by maintaining levels of vitamin E in the blood and tissues, and vitamin E is also a pronounced stimulant of the immune system (See A. Bendich, PD′Apolito, E. Gabriel, IJ Machlin. Modulation of the immune system function of guinea pigs by dietary vitamin E and C following exposure to oxygen // Fed. Proc. - 1983. - No. 42. - P.923).

The implementation of the invention

Examples of a specific test of the drug for the correction of stress conditions in animals.

Example 1

The drug for the correction of stress conditions in animals obtained in the following way: the stabilizer polyvinylpyrrolidone is added to an aqueous solution of H ₂ SeO ₃ , mixed under aseptic conditions with an aqueous solution of ascorbic acid taken in a 5-10% excess relative to equivalent, an aqueous solution containing selenium is obtained ( Se ⁰ ) and ascorbic acid in the following ratio of components in wt.%:

selenium (Se ⁰ ) 0,0005 ascorbic acid 3.0 water for injections rest

When analyzing the morphological and biochemical parameters of the blood of rabbits after using the drug, no significant differences were found in some indicators of the hematopoiesis system and the enzymatic link of the antioxidant system.

Example 2

Carried out analogously to example 1, but take the following ratio of components in wt.%:

selenium (Se ⁰ ) 0.001 ascorbic acid 5,0 water for injections rest

The resulting preparation, when used in rabbits in the analysis of morphological and biochemical blood parameters, shows a positive trend in animals. Red blood counts are subject to the greatest changes - the number of red blood cells increased by 13%, and the hemoglobin content by 12.3%. Catalase blood activity is increasing. The hepatic indicator of alanine aminotransferase (AlAT) is found to be normal in all animals.

Example 3

Carried out analogously to example 1, but take the following ratio of components in wt.%:

selenium (Se ⁰ ) 1,0 ascorbic acid 20.0 water for injections rest

The resulting preparation, when used in rabbits in the analysis of blood biochemical parameters, shows that the preparation has a pronounced stimulating effect on the functioning of the antioxidant system, which is expressed in an increase in catalase activity of 34.6% and a decrease in the rate of malondialdehyde by 28.7%, this ensures that the activation of peroxide processes is suppressed lipid oxidation and damage to the animal organism is prevented by lipid peroxidation products.

Example 4

Carried out analogously to example 1, but take the following ratio of components in wt.%:

selenium (Se ⁰ ) 2.0 ascorbic acid 25.0 water for injections rest

The resulting preparation, when applied to rabbits in the analysis of blood biochemical parameters, shows that an increase in the number of components leads to an insignificant manifestation of toxic effects. So, 7 days after the use of this drug, the activity of the enzymes alanine aminotransferase (AlAT) and aspartate aminotransferase (AsAT) increases by 24.3% and 16.5%, respectively, and the content of malondialdehyde also increases by 26%.

Thus, the most optimal doses of the drug is the drug according to examples 2, 3, which was tested on laboratory white mice, horses.

To evaluate the effect of the drug on hematological parameters, laboratory white mice are used; animals of the experimental groups are injected intramuscularly with the test drug in various doses.

Table 1 Hematological blood counts of white mice (n = 5) Group Dose, mg / kg DV Red blood cells, mln / μl Color indicator, units Hemoglobin, g / l White blood cells, thousand / μl the control-
naya - 7.5 ± 0.7 1.1 ± 0.09 151 ± 7 5.3 ± 1.1 1 experienced 0.7 8.0 ± 1.3 1.05 ± 0.04 160 ± 2 8.1 ± 0.8 2 experienced 1.3 8.5 ± 1.1 1.08 ± 0.07 158 ± 11 6.0 ± 1 3 experienced 2.6 6.5 ± 0.5 1.13 ± 0.1 112 ± 19 9.12 ± 1.8

Analyzing the results, we note the positive effect of administering the drug for the treatment and prevention of stress conditions for farm animals at a dose of 1.3 mg / kg according to the active substance (DV) on some hematological parameters: an increase in the number of red blood cells, hemoglobin level, stabilization of the number of leukocytes. This served as a starting point for determining the dose of the drug in assessing the effect of the drug on the animal organism.

table 2 Changes in the biochemical parameters of the blood serum of horses after the use of the drug at a dose of 1.3 mg / kg in the Far East (M ± m), (n = 10) Glucose mmol / L Asat mkkat / l AlAT mkkat / l De ritis Catalase mccat / L MDA μmol / L The control To load 4.18 ± 0.69 0.79 ± 0.12 0.068 ± 0.04 13.64 ± 4.7 1.35 ± 0.1 3.69 ± 3.36 After loading 4.42 ± 1.11 0.817 ± 0.36 0.085 ± 0.07 11.62 ± 4.99 1.44 ± 0.2 5.07 ± 3.6 After single use before loading 3.8 ± 0.36 0.89 ± 0.21 0.066 ± 0.01 13.74 ± 2.6 0.85 ± 0.26 0.78 ± 0.52 Experience after the course of the drug To load 3.58 ± 1.89 0.56 ± 0.24 0.20 ± 0.05 2.93 ± 1.84 0.79 ± 0.001 3.48 ± 1.5 After loading 4.66 ± 2.25 0.56 ± 0.07 0.182 ± 0.07 3.52 ± 1.53 0.8 ± 0.02 3.19 ± 3.69

A decrease in AsAT by 29-33% in combination with a sharp drop in the De Ritis index - 4.7-7.2 times indicate an improvement in the cardiovascular system. An increase in ALT is considered as an increase in the alanine glucose pathway with the release of glucose from cells due to its dephosphorylation, as evidenced by an increase in glucose after a course of the drug. It is clearly seen that the increase in AlAT is actually autonomous from AsAT, and the De Ritis coefficient is determined only by AlAT. This just means the activation of some paths while inhibiting other paths to achieve some kind of balance, since AsAT is an indicator of catabolism pathways closer to the Krebs cycle, and AlAT is more peripheral with numerous intersections of metabolic pathways for the exchange of proteins, fats and carbohydrates.

Stabilization of catalase activity indicates a well-functioning antioxidant defense. A decrease in the MDA indicator after exercise is observed, this ensures that activation of lipid peroxidation processes is suppressed, and an MDA indicator after exercise is prevented, this inhibits activation of lipid peroxidation processes and the animal is prevented from being damaged by lipid peroxidation products.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- reduction of toxicity

- high availability for the body

- increase antioxidant effect

- reduction of production costs

- effective stress corrector and adaptogen

Claims (1)

A preparation for the correction of stress conditions in animals, including an inorganic form of selenium, water for injection, characterized in that it is additionally provided with ascorbic acid, and selenium in the nanoscale state and zero valency (Se ⁰ ) is used as the inorganic form of selenium in the following ratio of components, wt.%:
selenium (Se ⁰ ) 0.001-1.0 ascorbic acid 5.0-20.0 water for injections rest