RU2540506C2 - Preparation for treating and preventing alimentary anaemia in young pigs - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to veterinary science and aims at treating and preventing alimentary anaemia in young pigs. A preparation contains an iron dextran complex, nanosized and zero-valent selenium (Se⁰), vitamin E, vitamin B₁₂ and water in the following proportions, wt %: iron dextran complex 0.0001-80.0, selenium (Se⁰) 0.0001-5.0, vitamin E 0.0001-20.0, vitamin B₁₂ 0.0001-10.0, water for injections - the rest.

EFFECT: using the declared method provides compensating iron deficiency in young pigs, improving animal's growth and development, increasing total immunity and rapid adaptation to the varying ambient environment.

4 tbl, 4 ex, 4 dwg

Description

FIELD OF THE INVENTION

The invention relates to veterinary pharmacy, namely, a drug for the treatment and prevention of nutritional anemia in piglets, and can be used in the prevention and treatment of iron deficiency diseases in piglets.

State of the art

The complex polysaccharide preparation Ursoferran is known, which makes up for iron deficiency in piglets. The advantage of Ursoferran is its stable binding of iron, with the help of which good local and general tolerance is achieved. The composition of the drug: a complex of polysaccharide and iron (III) hydroxide (iron 100 mg / ml), phenol 0.5 g / 100 ml (see Eliseeva, E. Healthy young growth - the basis of well-being of the economy / E. Eliseeva // Pig production. - 2008 . - No. 4. - S.24-28).

The disadvantages of this drug are the high cost and lack of vitamins.

Known iron-containing drug, which provides for a double intramuscular injection of iron preparations at a dose of 2-8 ml / goal for the first 10 days of animal life (100-375 mg Fe). The proposed method replaces the repeated injection of iron with a solution containing zinc glycinate, copper glycinate and potassium iodide (see US Pat. RU No. 2135173, IPC A61K 31/295, A61K 31/30, A61K 31/315, A61K 33/18 publ. 27.08. 1999).

The disadvantages of this drug are high toxicity, lack of digestibility of iron.

Known drug for the prevention and treatment of iron deficiency anemia. The drug contains ferbitol, while additionally containing gelatin in the following ratio of components, wt.%:

ferbitol 80-99 gelatin 1-20

(see Pat. RU No. 2280457, IPC A61K 33/26, A61K 35/32, A61P 7/06, published on July 27, 2006).

The disadvantage of this method is poor digestibility.

Known drug for the prevention and treatment of anemia. The drug provides, in comparison with analogues, a decrease in the content of dextran in the dosage form and the stability of aqueous solutions of the agent in a concentration of from 1 to 99% for 24 months. The preparation contains irondextran and sorbitol in the following ratio of components, wt.%:

iron dextran 78-90 sorbitol 10-22

(see Pat. RU No. 2338516, IPC A61K 31/047, A61K 31/721, A61P 7/06, published on November 20, 2008).

The disadvantages of this drug are high toxicity, lack of digestibility of iron.

A known method for the prevention and treatment of nutritional anemia and the prevention of hypoplastic anemia in piglets. The method includes the introduction of feed preparations containing iron. At the same time, piglets from 30 days of age are additionally injected with a probiotic preparation - hydrolyzed milk whey in a volume of 2 ml per kilogram of body weight once a day with a preventive purpose for 1 month. For therapeutic purposes, hydrolyzed milk serum is administered to piglets for 2 months in the same doses. The method contributes to the normalization of microbiocenosis, the activation of the enzymatic activity of the gastrointestinal tract by increasing the bifidogenic factor, increasing the digestibility of feed and bioavailability of protein-mineral-vitamin complexes introduced into these feeds (see US Pat. No. 2429863 IPC A61K 35/20, A61K 33 / 26, published September 27, 2011).

The disadvantages of this drug are the high cost, lack of vitamins, lack of digestibility of iron.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a complex microelement preparation of sedimia, which normalizes metabolism, compensating for the deficiency of iodine, selenium and iron in the body of piglets (see Alekseev V.V., Arestova I.Yu. Features biochemical picture of the blood of pigs with the appointment of "Permaite", "Calcephit-5" and "Sedimin®" // Bulletin of ChSPU named after Y. Yakovlev. - 2010. - No. 1 (65). - P.4-70).

Composition of the drug: 1 ml contains

iron (in the form of a complex of iron (III) with dextran) 16-20 mg iodine 5.5-7.5 mg selenium 0.07-0.09 mg

The disadvantages of this drug are the low level of effect on the body, as well as the increased sensitivity of piglets to iron-dextran preparations against the background of vitamin E deficiency.

Disclosure of invention

The objective of the invention is to develop a drug for the treatment and prevention of nutritional anemia in piglets, replenishing iron deficiency in piglets, improving the growth and development of animals, contributing to an increase in general immunity and rapid adaptation to changing environmental conditions.

The technical result that can be achieved using the present invention is to compensate for iron deficiency in piglets, improve the growth and development of animals, increase overall immunity and quickly adapt to changing environmental conditions.

The technical result is achieved using a drug for the treatment and prevention of nutritional anemia in piglets, including irondextran complex, selenium in the nanoscale state and zero valence (Se ⁰ ), vitamin E, vitamin B ₁₂ and water in the following ratio of components in wt.%:

irondextran complex 0.0001-80.0 selenium (Se ⁰ ) 0.0001-5.0 vitamin e 0.0001-20.0 vitamin b ₁₂ 0.0001-10.0 water for injections rest

The essence of the preparation for the prevention and treatment of alimentary anemia in piglets is as follows: starting materials in wt.%, Namely iron-dextran complex - 0.0001-80.0; selenium (Se ⁰ ) 0.0001-5.0; Vitamin E 0.0001-20.0; vitamin B ₁₂ 0.0001-10.0 and water for injection - the rest is mixed under aseptic conditions and packaged.

The pharmacological effect of the drug is that it compensates for the deficiency of iron by entering from the outside, while creating conditions for the accumulation of iron and an increase in its level in blood plasma, bone marrow, and erythrocytes.

Iron is an essential biochemical component in key processes of cell metabolism, growth and proliferation. The exclusive role of iron is determined by the important biological functions of proteins, which include this biometal. The most famous iron-containing proteins include hemoglobin and myoglobin. In addition to the latter, iron is a part of a significant number of enzymes involved in energy production processes (cytochromes), in DNA biosynthesis and cell division, detoxification of endogenous decomposition products that neutralize reactive oxygen species (peroxidase, cytochrome oxidase, catalase). The role of iron-containing proteins (ferritin) in the implementation of cellular immunity, the regulation of hematopoiesis is established. However, iron can be an extremely toxic element if it is present in the body in concentrations exceeding the capacity of iron-containing proteins. The potential toxicity of free ferrous iron (Fe ^II ) is explained by its ability to initiate chain free radical reactions leading to lipid peroxidation of biological membranes and toxic damage to proteins and nucleic acids. The main value of iron stores in the body is that they allow the hematopoietic system to adequately respond to acute and chronic blood loss (see E.F. Morshchakova, A.D. Pavlov / Regulation of iron homeostasis // Hematology and Transfusiology: Scientific and Practical Journal . - 2003. - No. 1. - S. 36-39).

Iron deficiency in the body causes various disorders of physiological functions: it reduces the level of oxygen carriers, disrupts the hematopoietic function of the bone marrow, reduces the level of enzymatic redox and immunobiological processes, and can lead to premature birth, undeveloped fetus, or its asphyxiation, and violation of labor. If iron absorption processes are disrupted, and iron reserves are depleted, then a state of iron deficiency occurs and pathology - iron deficiency anemia develops, enzymatic, redox and immunobiological processes are violated (see Belous AM Physiological role of iron / AM Belous, K.T. Konnik. Kiev : Naukova Dumka, 1991 .-- 104 p.).

On average, it is estimated that 20-25% of piglets or three pigs from each litter die due to this disease. It is assumed that 1/3 of the losses in pig breeding are caused by alimentary anemia (see Alikaev V.A., Ivanov D.P. New in the prevention and treatment of alimentary anemia in piglets. - In the book: Prevention and treatment of diseases of young animals of s.x. animals. - M .: Kolos, 1968, p. 222-243).

Modern breeds of domestic pigs are very prolific and have high growth energy. The relative growth rate of pigs increases sharply not at the end of their intrauterine development, but in the first month after birth (see Svechin KB, Individual Development of Farm Animals. - Ed. UASHA, Kiev, 1961).

If a normally developed piglet is born with a live weight of 1.1-1.2 kg, then by the time of weaning it can increase by 16-20 times, and by 6-7 months - by 50-60 times. Of all species of farm animals, piglets have the highest growth rate in the first months after birth. The physiological maturity of pigs under optimal conditions of feeding and keeping occurs by 10-11 months, and at 14-15 months you can already get a full litter (see Karput I.M., Abramov S.S., Scherbakov G.G., Levchenko V .I., I.P. Kondrakhin, A.V. Korobov, M.P. Babin, A.P. Kurdeko, I.Z. Sevryuk, S.L. Boroznov; Internal non-communicable animal diseases: textbook / ed. Prof. I.M. Karput. - Mn .: Belarus, 2006. - 679-680 p.). The main cause of anemia is the insufficient intake of iron in the body of piglets. In newborn animals, the iron content in the body is 48-50 mg. The need of piglets for this microelement in the first weeks of life is 10-15 mg per day, and sow's milk contains only 0.899 mg / l. Therefore, a piglet receives only 1-1.5 mg of iron per day with mother’s milk and its need is satisfied only by 12-15% (see Karput I.M., Abramov S.S., Scherbakov G.G., Levchenko V .I., I.P. Kondrakhin, A.V. Korobov, M.P. Babin, A.P. Kurdeko, I.Z. Sevryuk, S.L. Boroznov; Internal non-communicable animal diseases: textbook / ed. Prof. I.M. Karput. - Mn .: Belarus, 2006. - 679-680 p.).

Even with abundant feeding of sows with preparations containing iron, the amount of this trace element does not increase above a certain level. Less than 2% of the iron introduced into the body of pregnant sows penetrates the placenta and passes into the fetus. Therefore, that insignificant supply of it in the body of newborn piglets is quickly consumed, which leads to iron deficiency, which caused anemia. So, a piglet up to three weeks of age needs 114 mg of iron, and it receives about 24 mg with mother’s milk, and therefore, in newborns, the amount of hemoglobin sharply decreases in the first days of life: from 10.7 mg to ml to 4.5 mg. Therefore, already by the 7-8th day of life, piglets are deficient in iron, and by 3-4 weeks of age, the signs of anemia reach their greatest severity.

Selenium is an indispensable biologically active element for the life of the body. The trace element selenium plays an important role in the growth of young animals, the development and reproduction of animals, and affects the processes of tissue respiration. Selenium is actively involved in various metabolic processes, provides antioxidant protection for the animal organism, and the activity of many redox enzymes and vitamins. The ability to inhibit lipid peroxidation processes explains its role in enhancing the body's natural resistance.

Most researchers agree that selenium has a stimulating effect on the activity of the humoral immune system (see Fisinin, V. Natural minerals in animal and bird feeding / V. Fisinin, P. Surai // Russian Livestock. - 2008. - No. 9. - S. 62-63). There is evidence of the participation of selenium in immune reactions in the composition of selenoproteins, the exchange of thyroid hormones, which allows the use of selenium-containing drugs as immunostimulants. The trace element selenium has a high biochemical activity (see Lykasova I., Merenkova S. Efficacy of the drug nutril selenium in young fattening pigs // Pig production. - 2006. - No. 1. - P. 14-16), while selenium taken in nanoscale state and zero valency (Se ⁰ ) on a stabilizing basis of iron-dextran complex, contributes to the rapid absorption of the drug and metabolic intensification in animals.

Vitamin E, being an active natural antioxidant, is deposited mainly in the liver and adipose tissue. It stops the formation of lipid peroxides in cell membranes, while maintaining their integrity and functional activity. Vitamin E is a component of biological membranes, forming a complex with selenium and polyunsaturated acids in them. The more membranes of these acids, the greater the need for vitamin E and selenium to maintain their activity. By preserving fatty acids in platelet membranes, vitamin E prevents the formation of endoperoxides, precursors of prostaglandins, and this limits platelet aggregation processes. Vitamin E activates the synthesis of heme, which is part of hemoglobin, myoglobin, catalase, peroxidase, cytochromes. Increasing heme synthesis, vitamin E activates erythropoiesis, the synthesis of respiratory tissue enzymes, and therefore the energy and synthetic processes in them.

Vitamin B ₁₂ is a cobalt-containing complex compound. It regulates the process of hematopoiesis, the process of transmethylation of choline, methionine, creatine, nucleic acids and nucleotides, and also promotes the accumulation of compounds containing sulfhydryl groups in red blood cells. Methionine helps increase the biological value of plant diets (contributes to better absorption of amino acids of plant proteins).

Vitamin B ₁₂ is a growth factor, has a beneficial effect on the liver and nervous system. Vitamin B ₁₂ takes part in hematopoiesis, synthesis of methyl groups, isomerization of methylmalonic acid. These reactions are of great importance in the metabolism of amino acids, as well as volatile fatty acids. It is especially necessary for normal reproduction in sows, their provision prevents abortion, allows you to get healthy, well-developed piglets.

Brief description of drawings and other materials

In FIG. 1 is given a drug for the treatment and prevention of nutritional anemia in piglets, the effect of the drug on the change in body weight of rats, g (n = 10), table 1.

In FIG. 2 - the same, hematological parameters of white mice (n = 10), table 2.

In FIG. - 3 the same, biochemical parameters of blood serum of white mice (n = 10), table 3.

In FIG. 4 - the same, hematological indicators and the level of iron in blood serum of piglets, table 4.

The implementation of the invention

Examples of specific performance of obtaining the drug for the treatment and prevention of nutritional anemia in piglets.

Example 1. The essence of obtaining the drug for the prevention and treatment of nutritional anemia in piglets is as follows, the starting materials are iron-dextran complex, selenium in the nanoscale state and zero valency (Se ⁰ ), vitamin E, vitamin B ₁₂ and water in the following ratio of components in wt. %:

irondextran complex 0.00001 selenium (Se ⁰ ) 0.00001 vitamin e 0.00001 vitamin b ₁₂ 0.00001 water for injections rest

This composition of the dosage form showed a slight increase in the percentage of iron in the animal body necessary to eliminate iron deficiency, as well as insufficient activation of the antioxidant system of the body, as the activity of catalase in the blood of experimental rabbits increased by only 1.14%, and the concentration of malondialdehyde decreased by 0, 52%

Example 2. Carried out analogously to example 1, but take the following ratio of components in wt.%:

irondextran complex 20,0 selenium (Se ⁰ ) 3.0 vitamin e 15.0 vitamin b ₁₂ 7.0 water for injections rest

This composition of the dosage form showed a positive trend in animals. After 14 days, the activity of catalase and peroxidase in the blood of rabbits increased by 36.1% and 28.8%, respectively. The percentage concentration of iron after 5 days was 10.3 μg%. Serum cholesterol decreased by 10.5%, and total protein content increased by 11.2%.

Example 3. Carried out analogously to example 1, but take the following ratio of components in wt.%:

irondextran complex 80.0 selenium (Se ⁰ ) 5,0 vitamin e 20,0 vitamin B ₁₂ 10.0 water for injections rest

This composition of the dosage form also showed positive dynamics in animals. After 14 days, the activity of catalase and peroxidase in the blood of rabbits increased by 37.1% and 29.8%, respectively. The concentration of percent iron after 5 days was 11.3 μg%. Serum cholesterol decreased by 11.5%, and total protein content increased by 12.2%.

Example 4. Carried out analogously to example 1, but take the following ratio of components in wt.%:

irondextran complex 85.0 selenium (Se ⁰ ) 7.0 vitamin e 23.0 vitamin b ₁₂ 12.0 water for injections rest

An increase in the concentration of selenium and iron-dextran complex led to the manifestation of toxic effects. So, 5 days after the use of this drug, the activity of aspartate aminotransferase and alanine aminotransferase increased by 12.9 and 10.3%, respectively, the total bilirubin content also increased by 9.7%, which indicates an increased load on the liver.

Thus, the most optimal composition of the dosage form of the drug is the drug according to examples 2 and 3, which was tested on laboratory white mice, rabbits, pigs.

To determine lethal doses of the drug, clinically healthy white mice weighing 20-22 g are used, studies are carried out according to the “Guidelines for the toxicological evaluation of new drugs for the treatment and prevention of non-communicable animal diseases” (All-Russian Research Institute of Non-communicable Animal Diseases, 1987).

Acute Toxicity Study

Studies show that the developed drug with enteral (intragastric) administration in doses of 1041.66 to 6250.0 mg / kg (a.a.) body weight for white mice does not cause visible clinical signs of toxicosis. After the administration of 6250.0 mg / kg in the first two hours of the experiment, the mice showed anxiety, agitation, and twitching of individual muscle groups. These changes were apparently due to stress after the introduction of the probe into the stomach. Gradually, these phenomena disappear, and in animals the previous activity is restored.

Tested doses are significantly superior to therapeutic. A further increase in the administered dose is not possible, since the maximum volume of intragastric administration of preparations for white mice cannot exceed 0.6 ml; therefore, it was not possible to establish the exact values of MTD, LD ₅₀ , and LD ₁₀₀ . In relation to the maximum administered dose of the drug to the intended therapeutic, the therapeutic index is 20.

As a result of the studies, it was found that the drug data on the degree of exposure to the body in accordance with GOST 12.1.007-76 standards belong to the 4th hazard class - low-hazard substances.

Study of local action on the mucous membranes

The results of staging a conjunctival test in rabbits show that after the installation of the drug, the clinical condition of the animals remains within the physiological norm. No changes in the blood supply to the vessels of the conjunctiva, the presence of lacrimation and secretions, changes in the state of the cornea and eyelids.

The study of allergenic properties

Studies have shown that with sensitization of the animal organism through daily applications of drugs for ten days, no changes in the general condition of animals, as well as local changes in the skin (edema, hyperemia, etc.) are noted.

The study of chronic toxicity

Daily oral administration of an iron-containing preparation for 30 days to white rats at doses of 1.5 and 4.5 ml / kg of body weight does not cause changes in the behavior and general condition of the animals. When comparing the total and average daily weight gain of rats of the experimental group with rats of the control group, no significant differences were found. Also, no significant differences were found in the total and average daily weight gain of rats depending on the dose received (Table 1).

Study of the effect of the drug on the hematological parameters of white mice

The experiment was conducted on 30 clinically healthy white non-linear laboratory male mice weighing 25-30 g. To assess the effect of the drug on the hematological parameters of white mice, they were divided into three equivalent groups; animals of the control group are injected orally with physiological saline at a dose of 1.5 ml / kg body weight, the first experimental group is administered orally the test drug at a dose of 15 ml / kg body weight (ten times the therapeutic dose), and the second is 1.5 ml / kg body weight ( therapeutic dose). After 96 hours, the animals are decapitated and blood is collected for research. All hematological and biochemical studies are carried out according to generally accepted methods (see Menshikov VV Laboratory research methods in the clinic. Reference book. M: "Medicine", 1987. 368 S.).

Digital material is processed by methods of variation statistics. The reliability of the influence of a number of gradations of the studied factor (MEA toxicant) on hematological indices was revealed using the method of variance analysis of one-factor complexes of small groups (see Lakin GF Biometrics. Textbook for universities and pedagogical institutes. M: Higher school, 1973. 343 s). The significance of differences is determined by the Fisher F-test. The difference is considered significant at p <0.05 (table. 2).

As a result of the analysis of the results, no significant differences were found in the number and percentage of leukocytes, lymphocytes, monocytes in mice of the experimental and control groups. In the group of mice that received a ten-fold dose of the drug, a significant increase in the absolute number and percentage of granular leukocytes, respectively, by 45.5 and 39.9%, and a decrease in the number of lymphocytes were found. Granulocytes are important for the detoxification of various protein bodies, and are also directly involved in allergic reactions of the body.

Analyzing the obtained results, it is possible to note the positive effect of the drug administration for the treatment and prevention of diseases associated with iron deficiency for farm animals at a dose of 1.16 mg / kg in DV on some hematological parameters: an increase in the number of red blood cells, hemoglobin level, stabilization of the number of leukocytes. It was found that the administration of the drug increases the concentration of hemoglobin in mice by 10.7% in the second group and by 13.4% in the first, and helps to increase the number of red blood cells by 11.5% and 16.8%, respectively. This helps to increase the average content and average concentration of hemoglobin in red blood cells.

When analyzing the biochemical parameters of blood serum, a significant increase in the iron content in mice of the second experimental group was found to be 12.3 μmol / l, in mice of the third experimental group - by 7.5 μmol / l.

In the group of animals that received a ten-fold therapeutic dose, changes were noted (Table 3).

An increase in the content of the final product of protein metabolism - creatinine by 13.8% indicates an increase in energy metabolism in muscle and other tissues.

The use of the drug at a dose of 15.0 ml / kg in the blood of mice increases the activity of aspartate aminotransferase by 18.5%, and alanine aminotransferase by 21.9%. These results reflect a tendency toward an increase in liver function by transamination of amino acids.

An increase in amylase by 13.1% and triglycerides by 19.6% indicates an increase in pancreatic function.

It is known that albumin is involved in mineral, pigment, hormonal and some other types of metabolism, regulating the content of free (non-protein related fractions) biologically important substances with higher activity. Due to this function, albumin plays a significant role in the implementation of detoxification processes, which explains its decrease in the second group by 19.7%.

The introduction of the drug increases the total protein content in mice of the second and third groups, respectively, by 10.35 and 13.36%. In the body, the total protein performs the following functions: participates in blood coagulation, maintains a constant blood pH, performs a transport function (transfer of fats, bilirubin, steroid hormones to tissues and organs), and participates in immune reactions.

Hepatocytes absorb bilirubin only in a bound state with albumin, since indirect (free bilirubin) is a poorly soluble compound in water. Accordingly, with a decrease in albumin in the blood, the absorption of bilirubin by hepatocytes decreases, which leads to the accumulation of bilirubin in the blood of mice of the second group within physiological values.

At autopsy, the color, size, elasticity, and mass of the internal organs of the experimental and control mice were the same.

Study of the effect of the drug on the hematological parameters of piglets

Clinical studies are carried out on 60 piglets of the breed SM-1 at the age of 4-30 days. Animals were divided into three groups, 20 animals each. Animals in the groups were selected on the basis of “analogues” (weight, feeding and keeping conditions). Each group of animals was formed from separate nests.

Animals of the 1st experimental group - on the 4th day of life, the developed drug is injected intramuscularly once in a dose of 1.5 ml per animal. Other ingredients: aqueous solvent. For pigs of the second group, the drug is administered on the 4th day of life once intramuscularly in the region of the upper third of the thigh at a dose of 2 ml per animal injected with the drug ursoferran-100, containing 1 ml of 100 mg of iron in the form of iron-dextran-heptonic acid. Piglets of the third group were not injected with drugs and they served as a control.

During the experiment three times: at the age of 4, 15 and 30 days, some morphological and biochemical parameters of the blood of piglets are studied.

The results of blood tests of experimental piglets are presented in table 4.

A study of a number of hematological parameters of the experimental animals made it possible to establish that in piglets that received the developed preparation and ursoferran-100 throughout the entire period of research, the average red blood cell count and hemoglobin level, gradually increasing with age, are within the standard physiological values. At the same time, in piglets of the first experimental group, these indicators slightly exceed the values of those in piglets of the second group: the hemoglobin level at the age of 15 days is 9.7%, at the age of 30 days it is 7.3%. The number of red blood cells in piglets of the first experimental group at the age of 15 days was 9.7%, at the age of 30 days it was 7.3% higher than similar values for animals of the second group.

In control piglets at the age of 15 days, the number of red blood cells and the hemoglobin concentration correspond to extremely low values, 4.12 + 0.28 × 10 ¹² / l and 56.7 ± 2.4 g / l, respectively, and continue to be low 30 days of age: 4.98 + 0.33 × 10 ¹² / l and 58.4 ± 2.8 g / l.

When studying the dynamics of the number of leukocytes in suckling piglets, the following was revealed: if in the experimental animals the average leukocyte count remained practically unchanged throughout the study period, fluctuating within the standard physiological values, then in piglets of the control group progressive leukopenia was observed, most pronounced at the age of 10-12 days, which indicates inhibition of leukopoiesis and a decrease in body resistance, characteristic for iron deficiency anemia.

The use of iron-containing preparations prevented a sideropenic state in the body of experimental piglets, maintaining the content of serum iron at the level of standard physiological parameters. At the same time, throughout the entire period of the experiment, the content of serum iron in the first group of piglets was higher compared to animals of the second group, which are injected with ursoferran, after 26 days after administration - by 11.8%.

In piglets of the control group, the level of iron in the blood serum for a specified period of time did not reach physiological normative values, ranging from 11.4 ± 0.4 to 13.6 ± 0.4 μmol / L, respectively, at 15 and 30 days of life, and erythropenia and hemoglobinemia and was accompanied by the development of clinical signs of iron deficiency anemia.

Thus, the testing allows us to conclude that the use of the developed drug is an effective way to prevent nutritional anemia in piglets.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- Replenishment of iron deficiency in piglets;

- improving the growth and development of animals;

- increase in general immunity and rapid adaptation to changing environmental conditions.

- high preventive and therapeutic effect.

Claims (1)

The drug for the prevention and treatment of nutritional anemia in piglets, including irondextran complex, selenium in the nanoscale state and zero valence (Se ⁰ ), vitamin E, vitamin B ₁₂ and water in the following ratio of components in wt.%:
irondextran complex 0.0001-80.0 selenium (Se ⁰ ) 0.0001-5.0 vitamin e 0.0001-20.0 vitamin b ₁₂ 0.0001-10.0 water for injections rest

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