RU2406373C2 - Feed supplement and its production method - Google Patents

Abstract

FIELD: food industry. ^ SUBSTANCE: invention relates to animal feed materials production. The feed supplement contains a non-separated yeast autolysate, a flavouring material, enzyme preparation Celloviridin G20x or Fekord U4 produced based on Trichoderma reesei (viride) VSM 18.2/KK fungus strain or enzyme preparation Xibeten-Xyl or Xibeten-Cel produced based on Trichoderma longibrachiatum TW-1 fungus strain, culinary salt, sodium selenite, potassium iodide, feed chalk and a filler represented by products of mill waste. For the feed supplement preparation one preliminarily mixes flavouring material, filler (represented by products of mill waste) culinary salt till homogeneous mass production; the produced mixture is maintained during 1.5-2 hours, then one adds non-separated yeast autolysate and stirs till homogeneous mass production; one maintains the mass during 1.5-2 hours, adds to the produced mixture enzyme preparation produced based on Trichoderma reesei (viride) VSM 18.2/KK fungus strain or Trichoderma longibrachiatum TW-1 fungus strain, sodium selenite, potassium iodide, feed chalk stirs till homogeneous mass production. ^ EFFECT: invention allows to enhance nutritional value of the feed supplement and animal productivity. ^ 2 cl, 5 tbl, 4 ex

Description

The invention is intended for use in agriculture in the production of feed for animals.

Known feed additive obtained by autolysis of pressed baking yeast with a solids content of at least 25%, placing them in a thermostat and holding at a temperature of 50 ± 5 ° C for 30 ± 5 hours. In addition to the yeast autolysate, the feed additive may consist of a filler - bran of grain crops (US Pat. RF No. 2229243, IPC A23K 1/06, 2004, BI No. 15).

The autolysate obtained as a result of yeast autolysis is of poor quality due to the incomplete depth of yeast autolysis, as evidenced by the content of amminous nitrogen in the obtained autolysate, and also has limited eatability due to the specific smell of the autolysate and insufficient nutritional value.

There is also a method for producing yeast autolysate by heating an aqueous suspension of yeast at 45-55 ° C in the presence of an enzyme preparation obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / KK, in an amount of 0.05-0.15% of mass of absolutely dry yeast for 4-5 hours, the subsequent introduction of oleic acid in an amount of 0.1-0.3% of the mass of absolutely dry yeast and aging for 10-15 hours (US Pat. RF No. 2306714, IPC A23J 1/18 , C12N 1/06, C12R 1/885, 2007, BI No. 27).

When carrying out autolysis of yeast in the known method, separation and filtration are not performed, and the resulting solution with fragments of cell structures is sent for drying, which provided it with additional sorption properties, a rich group of water-soluble and fat-soluble vitamins (A ₁ , B ₁ , B ₂ , B ₃ , B ₄ , B ₅ , B ₆ , D ₃ , E), micro and macro elements.

Obtained by a known method, the yeast autolysate also has insufficient nutritional value, has a specific odor, and when introduced into the feed to a certain concentration limits its eatability.

The objective is to increase the eatability, nutritional value of feed additives and animal productivity.

The problem is solved in that the feed additive containing unseparated yeast autolysate additionally contains the enzyme preparation Celloviridin G20X or Fekord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / KK, or the enzyme preparation Xibeten-Cel or Xibeten -Xyl obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, flavor, table salt of sodium selenite, potassium iodide, feed chalk and filler - waste milling in the following ratio of components, wt. %:

unseparated yeast autolysate 40-70 enzyme preparation 2.0-6.6 flavoring 1.0-1.9 salt 0.3-0.5 sodium selenite 0.7-0.9 potassium iodide 1.0-2.7 feed chalk 0.5-1.0 filler rest

The unseparated yeast autolysate, which is part of the feed additive, enriches it with biologically active substances, is an additional source of free amino acids, easily digestible protein, vitamins A, D, E and group B. The iron and manganese, zinc, and copper contained in the yeast autolysate along with macro- and micronutrients , sodium, potassium, magnesium, phosphorus and calcium and the feed chalk included in the feed additive provide the necessary calcium-phosphorus metabolism and acid-base state in animals with Ca: P ratio (1.27-2.04): 1. The potassium iodide and sodium selenite, which are part of the feed additive, are important trace elements necessary for the life of the body, and sodium chloride provides replenishment in sodium feed, increases animal appetite and facilitates the course of acidosis. The flavoring agent included in the feed additive has a stable taste and odor, gives the feed components an attractive odor, stimulates the secretion of saliva and digestive enzymes, provides increased feed intake using various, including inexpensive feed components with an unattractive odor and taste. The enzyme preparation, which is part of the feed additive, when it enters the gastrointestinal tract, is involved in the destruction of fiber of components of plant-derived feed by enzymatic hydrolysis of glycosidic bonds of non-starch polysaccharides - cellulose, glucans, xylans and araboxylans (pentosans), which leads to the formation of fragments of lower molecular weight and lowering the viscosity of the chyme in the gastrointestinal tract.

The inventive feed additive in its composition provides the possibility of its use in feed for various groups of animals with limited use of additional components.

There is also known a method of preparing feed for farm animals, comprising mixing a yeast autolysate, feed chalk, salt, hop grains, protein sludge, brewer's yeast, suction and polishing waste, barley alloy, grain waste, malt sprouts and beer grains (US Pat. RF № 2075298, IPC A23K 1/16, BI No. 8, 1997).

The preparation of the inventive feed additives by simply mixing its components does not provide a solution to the problem.

The problem is also solved by the fact that in the method of preparation of the feed, including mixing the yeast autolysate, feed chalk, table salt, the components of the feed additive are mixed in the following order: pre-mixed filler - waste milling, flavoring, table salt and aged within 1.5-2 hours, then an unseparated yeast autolysate is introduced, mixed until a homogeneous mass is obtained and incubated for 1.5-2 hours and into the resulting mixture the enzyme preparation Celloviridin G20X or Fekord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / KK, or the enzyme preparation Xibeten-Cel or Xibeten-Xyl, obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, sodium selenite, potassium iodide, feed chalk and mixing until a homogeneous mass.

With this method of preparation of the feed additive, a relatively small mass of powdery flavoring agent, finely divided powdery unseparated yeast autolysate and filler in standard food mixers is mixed well, the aroma of the flavoring agent used is most fully saturated, and the components of the feed additive and feeds have a pleasant smell and taste.

The invention is illustrated by examples of tests on analog groups of cattle (cattle) and pigs, formed taking into account the age, sex, live weight, fatness and physiological condition of animals.

In the preparation of the feed additive, the flavoring oil-vanilla flavor (supplier Star-Samara TD, LLC) was used as a flavoring agent.

The compositions of the feed additives are shown in table 1, where No. 1 is the composition of the prototype, No. 3-6 are the compositions of the claimed feed additives.

Example 1

Groups of animals of cattle of black-motley breed were formed: groups of rearing in the amount of 10 animals, groups of fattening in the amount of 10 animals and groups of dairy cows in the amount of 10 animals.

The composition of the feed additive according to the prototype and the compositions of the inventive feed additive with the enzyme preparation XIBETEN-CEL, obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, were fed as part of the KR-2 compound feed (manufactured by Lidahleboproduct OJSC) to the growing groups in an amount of 40 g per head per day for 30 days, in the composition of the KR-3 compound feed group of feeding in the amount of 50 g per head per day for 30 days and groups of dairy cows in the composition of the KK-60C compound feed in the amount of 60 g per head per day for 30 days. The test results are summarized in table 2.

Example 2

Groups of large white breed pigs were formed: groups of suckling piglets of 20 goals each, groups of weaning piglets in the amount of 20 goals and fattening groups in the amount of 20 goals.

The composition of the feed additive according to the prototype and the compositions of the inventive feed additive with the enzyme preparation XIBETEN-CEL, obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, were fed to groups of animals: groups of suckling pigs in the composition of feed SK-11 in the amount of 1.5% by weight of feed within 45 days, to groups of weaning pigs in the composition of SK-16 compound feed in the amount of 1.5% to the mass of feed for 60 days and to the feeding groups in the composition of SK-26 compound feed in the amount of 1.5% to the mass of feed for 60 days . The test results are summarized in table 3.

Example 3

Groups of animals of cattle of black-motley breed were formed: groups of rearing in the amount of 10 animals, groups of fattening in the amount of 10 animals and groups of dairy cows in the amount of 10 animals.

The composition of the prototype and composition No. 6 (table 1) of the inventive feed additive with the enzyme preparation KSIBETEN-KSIL obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, with the enzyme preparation Cloveridin G20X and the enzyme preparation Fekord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / QC was fed to groups of animals, as in example 1. The test results are summarized in table 4.

Example 4

Groups of large white breed pigs were formed: groups of suckling piglets of 20 goals each, groups of weaning piglets in the amount of 20 goals and fattening groups in the amount of 20 goals.

The composition of the prototype and composition No. 6 (table 1) of the inventive feed additive with the enzyme preparation KSIBETEN-KSIL obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, with the enzyme preparation Cloveridin G20X and the enzyme preparation Fekord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / QC was fed to groups of animals, as in example 2. The test results are summarized in table 5.

As can be seen from tables 2-5 of the test data of the compositions of the inventive feed additives with enzyme preparations Cloveridin G20X or Fecord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / KK, and enzyme preparations Xibeten-Cel or Xibeten-Xyl obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, the average daily gain in comparison with the prototype calves in the growing groups increased by 13.7-15.1%, in the fattening groups - by 17.8-20.9%, the average daily milk yield of the group of dairy cows increased in comparison with the prototype by 9.5-12.7%. The average daily gain increased in comparison with the prototype in the groups of suckling piglets by 15.5-18.8%, in the groups of weaned piglets - by 11.3-16.0% and in the feeding groups - by 15.3-18.6% .

In the groups of animals that were fed a feed additive according to the prototype, and in the groups of animals that were fed the claimed feed additive, morbidity and mortality were observed. Refusal to take food with the claimed feed additive by animals, even with a maximum content of autolysate in it, was not observed.

Table 1 Name of components Compositions, mass,% I II III IV V VI VII Unseparable yeast autolysate thirty thirty 40 fifty 60 70 80 Enzyme preparation - 1.8 2.0 4.2 5,4 6.6 7.8 Salt - 0.1 0.3 0.35 0.4 0.5 0.6 Sodium Selenite - 0.5 0.7 0.75 0.8 0.9 1,0 Potassium iodide - 0.9 1,0 1.9 2,3 2.7 3,1 Feed chalk - 0.3 0.5 0.7 0.9 1.4 1.3 Taste flavor - 0.5 1,0 1.3 1,6 1.9 2.2 Filler 70 65.9 54.5 40.9 28.6 16,4 4.0

table 2 Composition number The average daily gain of calves, g / day The average daily milk yield of a group of dairy cows, l / day Growing group Fattening group one 820 980 15.0 2 825 1030 15.1 3 870 1105 15,5 four 923 1150 16.1 5 933 1172 16.8 6 944 1185 16.9 7 943 1186 16.8

Table 3 Composition number The average daily gain of pigs of various age groups, g / day Group of suckling piglets Group of weaned piglets Fattening group one 240 495 593 2 243 502 596 3 258 543 624 four 269 558 680 5 277 569 694 6 285 574 703 7 284 574 701

Table 4 Composition number The average daily gain of calves, g / day The average daily milk yield of a group of dairy cows, l / day Growing group Fattening group one 820 980 15.0 2 944 1185 16.9 3 941 1179 16.8 four 932 1154 16,4 5 938 1163 16.6

Table 5 Composition number The average daily gain of pigs of various age groups, g / day Group of suckling piglets Group of weaned piglets Fattening group one 240 445 593 2 285 574 703 3 282 569 695 four 277 551 683 5 279 558 689

In tables 4 and 5:

composition No. 1 - composition of the prototype;

composition No. 2 - the claimed composition of the feed additive with the enzyme preparation Xibeten-Cel (examples 6 of tables 2 and 3);

composition No. 3 - the claimed composition of the feed additive with the enzyme preparation Celloviridin G20x;

composition No. 4 - the claimed composition of the feed additive with the enzyme preparation Fecord U-4;

composition No. 5 - the claimed composition of the feed additive with the enzyme preparation Xibeten-Xil.

Information sources

1. RU 2306714 C2, 09.27.2007.

2. RU 2229243 C1, 05.27.2004.

3. RU 2075298 C3, 03.20.1997.

Claims (2)

1. A feed additive containing an unseparated yeast autolysate, characterized in that it additionally contains the enzyme preparation Celloviridin G20x or Fekord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / KK, or the enzyme preparation Xibeten-Xyl or Xibeten-Cel, obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, table salt, flavor, sodium selenite, potassium iodide, feed chalk and filler - waste milling in the following ratio, wt.%:
unseparated yeast autolysate 40-70 enzyme preparation 2.0-6.6 flavoring 1.0-1.9 salt 0.3-0.5 sodium selenite 0.7-0.9 potassium iodide 1.0-2.7 feed chalk 0.5-1.0 filler rest 2. A method of preparing a feed additive, comprising mixing a yeast autolysate, feed chalk, table salt, characterized in that the components of the feed additive are mixed in the following order: pre-mixed filler - flour mill waste, flavor, table salt and kept in for 1.5-2 hours, then an unseparated yeast autolysate is introduced, mixed until a homogeneous mass is obtained and incubated for 1.5-2 hours, and the enzyme is introduced into the resulting mixture Arat Celloviridin G20x or Fekord U-4, obtained on the basis of the strain of the fungus Trichoderma reesei (viride) BCM 18.2 / KK, or the enzyme preparation Xibeten-Xyl or Xibeten-Cel, obtained on the basis of the strain of the fungus Trichoderma longibrachiatum TW-1, sodium selenite , potassium iodide, feed chalk and mixed until a homogeneous mass.