RU2314108C1 - Method for correcting free radical oxidation and antioxidant protection in cows at technological stress - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with introducing inorganic compounds as Khotynets' natural zeolites in the form of fodder additive during feeding at 4% against the main diet/animal in the course of winter stable period of keeping. The innovation enables to decrease the level of malonic dialdehyde and increase the level of ceruloplasmin in animals during winter stable period.

EFFECT: higher efficiency of correction.

2 ex, 2 tbl

Description

Known methods for the correction of free radical oxidation and antioxidant protection, including the introduction of a number of selenium-containing compounds, such as selenites and selenates, selenopyran, selenophen, selecor (Degtyarev D.V., Alekhin Yu.N., Kurkin S.V., Faustov A. I. The effect of organic and inorganic compounds of selenium on weight gain and antioxidant protection in calves // Veterinary Pathology No. 3 (7), 2003, pp. 70-71). - [one]

A disadvantage of the known methods is that the means used, inorganic compounds of selenium (selenites and selenates) are very toxic (LD ₅₀ - 10 mg / kg), and organic (selenopyran, selenophen, selecor) less toxic (LD ₅₀ - 8100 mg / kg ), but expensive.

The objective of the proposed method is the use of non-toxic and inexpensive, highly effective means for the correction of free radical oxidation and antioxidant protection in cows.

The problem is solved due to the fact that in the known method of correction of free radical oxidation and antioxidant protection, an inorganic compound according to the invention is introduced as a corrective agent, and Khotynetsk natural zeolites of the Oryol region are introduced as inorganic compounds in the morning during feeding as free sorbents radicals and micronutrient sources at the rate of 4% of the main diet for 1 head under technological stress (physical inactivity, hypoxia and nutritional stress) in the winter stall.

The average mineral composition of the Khotynetsk natural zeolites as a percentage of the mass is as follows: SiO ₂ - 77.85, TiO ₂ - 0.57, Al ₂ О ₃ - 10.41, Fe ₂ O ₃ - 3.63, FeO - 0.23, MnO - 0.02, MgO - 1.32, CaO - 1.52, Na ₂ O - 0.23, K ₂ O - 1.70, P ₂ O ₅ - 0.14, SiO ₃ - traces.

Ready-to-eat zeolite flour complies with GOST GU 9318-001-96 and has a certificate of conformity No. ROSSRUPOV, AOO53 issued by the Orlovsky certification body for agro-industrial ecology and a temporary instruction on the use of Khotynets natural zeolites in animal and bird feeding, approved by the animal husbandry department and breeding of the Ministry of Agriculture of the Food of the Russian Federation.

According to the literature data and the results of our studies, zeolites have catalytic and sorption properties.

The essence of the invention consists in the following: for normalization (correction) of free-radical oxidation and antioxidant protection arising from technological stress (hypoxia, physical inactivity, nutritional stress), Khotnetsk natural zeolites of the Oryol region were used, which, being sorbents of free radicals and a source of mineral elements, normalize free radical oxidation and the antioxidant defense system.

Zeolites were added as a feed additive of 250 g per head (based on 4% of the main diet per head). Zeolites were added to an individual feeder by mixing with the main feed.

Treatment with Khotynetsk natural zeolites was carried out once a day during feeding.

The experiments were carried out in the winter stall period, characterized by technological stress (physical inactivity, hypoxia and alimentary stress).

During this period, cows have an abnormally high level of free-radical oxidation compared to summer pasture.

Comparative indicators of malondialdehyde (MDA), which characterizes the level of free radical oxidation and the antioxidant ceruloplasmin in the spring-summer and winter-stall periods.

Table 1 The analyzed periods of the year Animal number The indicators of malondialdehyde in a conventional unit Indicators of activity of the antioxidant ceruloplasmin in arbitrary units (unit optical density) Winter stall period one 0.08 0,050 2 0,07 0,055 3 0,07 0.056 four 0,075 0.058 5 0.08 0,050 Spring-summer period - (grazing period) one 0.04 0,07 2 0,03 0,073 3 0,038 0.72 four 0.04 0,074 5 0,035 0.73

The analysis of table 1 shows a pathologically high level of free radical oxidation by the level of MDA in the winter-stall period. To normalize the level of malondialdehyde, we used the Khotynetsk natural zeolites of the Oryol region.

Example 1

A cow with a live weight of 320 kg and an average milk yield for the last lactation of 2800 kg of milk was examined for free radical oxidation (in terms of malondialdehyde) and antioxidant protection (in terms of ceruloplasmin). The indicator of malondialdehyde at the beginning of the experiment was 0.75 units. opt. density, the rate of ceruloplasmin 0.054 units. opt. density. They fed the cows according to the basic ration of the farm. After 2 weeks, the level of malondialdehyde remained at the same pathologically high level, and the level of antioxidant protection was low.

Example 2

A cow with a live weight of 330 kg and an average milk yield for the last completed lactation of 2620 kg of milk was examined for free radical oxidation in terms of malondialdehyde and antioxidant protection in terms of ceruloplasmin. The indicator of malondialdehyde at the beginning of the experiment was 0.78 units. opt. density, the rate of ceruloplasmin was 0.058 units. opt. density. The cows were fed according to the main diet + 4% of the main ration of the farm (250 g), Khotynetsk natural zeolites were added as a feed additive. The level of malondialdehyde decreased in 2 weeks to 0.05 units. opt. density, and ceruloplasmin increased to 0.075 units. opt. density, which is typical for an animal without stress.

Comparative indicators of malondialdehyde and the antioxidant cerruplasmin in the winter stall period in the group of cows with and without the use of Khotnetsk zeolites in feeding.

table 2 The analyzed period of the year Group of cows Animal number Indicators of malondialdehyde in arbitrary units (unit opt. Pl.). Indicators of the activity of the antioxidant cerruloplasmin a in conv. one (unit wholesale pl.) Before the start of the experiment 2 weeks after the start of feeding with the Khotynetsk zeolites Before the start of the experiment 2 weeks after the start of the experiment Winter stall The first (without use in feeding Khotynetsk natural zeolites one 0.08 0.08 0.05 0,049 2 0,075 0,076 0,054 0.05 3 0.08 0,079 0.05 0.05 four 0,078 0.08 0.058 0,057 5 0,076 0,076 0,057 0.058 Winter stall The second (using Khotynetsk natural zeolites in feeding) one 0.08 0.06 0.05 0,07 2 0,07 0,048 0,055 0,075 3 0,07 0,045 0.056 0,07 four 0,075 0.056 0.058 0,075 5 0.08 0.06 0,050 0,074

The effectiveness of the method for correcting free radical oxidation and antioxidant protection in cows by the Khotynetsk natural zeolites of the Oryol region due to their sorption and catalytic properties is confirmed by a decrease in the level of free radical oxidation (in terms of malondialdehyde) and an increase in antioxidant protection (in terms of ceruloplasmin), which follows from analysis of tables 1 and 2.

Claims (1)

A method for the correction of free radical oxidation and antioxidant protection in cows under technological stress, including the introduction of inorganic compounds, characterized in that Khotynetsk natural zeolites are introduced as an inorganic compound in the form of a feed additive in the morning during feeding, at the rate of 4% of the main ration per head during the winter stall period.