RU2252418C2 - Method for diagnosing endotoxicosis condition in cows suffering from acute pyocatarrhal endometritis - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S_{238-298(plasma)}/S_{238-298(erythrocytes)}*GAC, where S_{238-298(plasma)} and S_{238-298(erythrocytes)} are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units²); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0.

EFFECT: high accuracy of diagnosis.

1 dwg, 1 tbl

Description

The present invention relates to the field of veterinary medicine and can be used to diagnose the severity of endotoxicosis of the body.

A known method for diagnosing the degree of endotoxemia, including measuring the degree of influence of the test blood serum on the filtering time of red blood cells of healthy donors. Toxicity is determined by the increase in the filtration time of donor erythrocytes after incubation with the serum of a sick body [A.S. USSR No. 1475333, class G 01 N 33/48].

One of the generally accepted methods for the diagnosis of endotoxemia is the determination of the binding function of serum albumin [A.S. USSR No. 1409931, class. G 01 N 33/48].

Barnhill Stephen Datall at al for the diagnosis of intoxication of the body offer a diagnostic index obtained by processing laboratory data [WO 9612187 class. G 01 N 33/53].

To increase the sensitivity of assessing the degree of endotoxemia, it is known to use data on the plasma content of medium-weight molecules and the effective concentration of albumin [V. B. Gavrilov. Assessment of organism intoxication by imbalance between the accumulation and binding of toxins in blood plasma / V. B. Gavrilov, M. M. Bidula, D. A. Furmanchuk, S. V. Konev, O. V. Aleinikova // Klin. lab. the diagnosis - 1999. - No. 2 - S.13-17]. If it is impossible to determine the effective concentration of albumin, the authors propose to determine the total concentration of albumin and calculate intoxication criteria (CI) using the formulas:

KI ₁ = 100D ₂₅₄ / OKA)

KI ₂ = 1000 · D ₂₈₀ / OKA), where

D ₂₅₄ , D ₂₈₀ - the content of medium-weight molecules, determined by the optical density of the plasma at wavelengths of 254 and 280 nm (conventional units);

OKA - total concentration of plasma albumin (g / l).

The disadvantage of the diagnostic method is its limitedness due to the use of only an imbalance between the accumulation and binding of toxins by albumin in blood plasma without taking into account the sorption function of red blood cells.

The objective of the invention is to increase the efficiency of assessing the degree of endotoxemia of the body.

The problem is solved in that in a method for diagnosing the degree of endotoxemia by examining blood plasma for the content of low and medium molecular weight substances (VNSSM) and the total concentration of albumin, the VNSSM content in erythrocytes is additionally determined, the integral index (II) is calculated based on the obtained parameters by the formula:

AI = 100 · S _{238-298 (plasma)} / S _{238-298 (red blood cells)} · OKA, where

S _{238-298 (plasma)} , S _{238-298 (erythrocytes)} - the content (VNSSM) of plasma and red blood cells, determined by the area of the figures bounded by spectral curves in the wavelength range of 238-298 nm and the abscissa axis (conv. Unit ² ) ;

OKA - total concentration of plasma albumin (g / l),

and with its value from 2.1-3.0, the first degree of endotoxemia is diagnosed, from 3.1-4.5 - the second, from 4.6 to 6.0 - the third degree, above 6.0 - the fourth degree of endotoxemia, with the norm is from 0.5 to 2.0.

In this regard, an integrated index has been developed to determine the degree of intoxication in cows with acute purulent-catarrhal endometritis based on an analysis of the dynamics of the following indicators: albumin, plasma BNSCM and BNSCM red blood cells. The total concentration of albumin was determined using the dye bromocresol green (“Lachema” Czech Republic), the content of VNSSMM was determined according to the method of M.Ya. Malakhova [Malakhova M.Ya. Method for recording endogenous intoxication. - A manual for doctors. - SBP: Publishing House of the St. Petersburg Medical Academy of Postgraduate Education. - 1995. - 34 p.]. According to the degree of intoxication, the animals were divided into 3 groups according to the severity of the condition. The 1st and 2nd groups included cows in a compensated state without threat and with the threat of sepsis, respectively. The third group consisted of animals with developed sepsis and a failure of any one or more functional systems (heart failure, respiratory failure, acute renal failure). The control group included cows of 2-3 years of red steppe breed with similar indicators of weight and milk productivity, in which the values of routine biochemical parameters were within the physiological norm. Mathematical processing of the results was carried out using the Student criterion, a comparative analysis of the samples using the Fisher criterion.

The profiles of the spectrograms of the plasma and red blood cells of animals of all the studied groups have approximately the same form. At wavelengths of 238-246 nm, zero optical density values are observed. Starting from 250 nm, the spectrograms have uniformly ascending values of optical density with absorption maxima at wavelengths of 274 ± 8 nm and 286 ± 4 nm for plasma and red blood cells, respectively (see drawing).

The average heights of the peaks of the spectrograms of plasma and red blood cells of healthy cows are respectively 0.144 and 0.383 srvc. units

The content of VNSSM in biological fluids allows us to judge the area of the figure bounded by the spectral curve and the abscissa axis. This indicator for the above group of animals has the following average values: for plasma - 5.5 ± 0.7, and for red blood cells - 15.1 ± 1.6 srvc. units ²

In the initial stage of endotoxemia on the spectrogram of cows with purulent-catarrhal endometritis, an increase in the sorption capacity of red blood cells is observed, because an erythrocyte due to the glycophorin protein adsorbs on its surface substances of different classes, including the average molecular weight. At the same time, the concentration of VNSMM in blood plasma also increases slightly. The values of the areas of the spectrograms of plasma and red blood cells are respectively 11.5 ± 1.2 and 20.3 ± 2.4 srvc. units ² In the spectrograms corresponding to the second stage of endotoxemia, there is a moderate increase in the content of VCSMM in both plasma and red blood cells. The values of the areas of the spectrograms of plasma and red blood cells are within 18.0 ± 1.8 and 26.7 ± 2.7 srvc. units ² respectively. At the third stage, there is a significant increase in the concentration of VNSMM in blood plasma, while on erythrocytes it practically remains unchanged. The values of the areas range from 25.3 ± 2.6 and 27.8 ± 2.6 conv. units ² for plasma and red blood cells, respectively.

The plasma albumin content of healthy cows during milk production is 31.4 ± 2.8 g / l. With the development of intoxication, the values of this indicator decrease.

The integral index of endotoxemia in cows with purulent-catarrhal endometritis is as follows:

AI = 100 · S _{238-298 (plasma)} / S _{238-298 (red blood cells)} · OKA, where

S _{238-298 (plasma)} , S _{238-298 (erythrocytes)} —the HPSMM content of plasma and erythrocytes, determined by the area of the figures bounded by spectral curves in the wavelength range of 238-298 nm and the abscissa axis (conventional units ² );

OKA - total concentration of plasma albumin (g / l).

The method is as follows: calculate the integral index according to the above formula. With its value from 2.1 to 3.0, the first degree of endotoxemia is diagnosed, from 3.1 to 4.5 - the second, from 4.6 to 6.0 - the third degree, above 6.0 - the fourth degree of endotoxemia. The index value is normal - from 0.5 to 2.0.

The proposed index was analyzed for 7 days for two groups of animals with the first and second degree of endotoxemia. The data are given in the table.

Table
The values of the integral blood index of cows with the first and second degree of endotoxemia The observation period, day Integral Index Values The first degree of endotoxemia Second degree of endotoxemia 1 2.7 ± 0.3 3.8 ± 0.4 2 2.6 ± 0.3 3.6 ± 0.4 3 2.5 ± 0.3 3.5 ± 0.3 4 2.4 ± 0.2 3.2 ± 0.3 5 2.1 ± 0.2 2.8 ± 0.3 6 1.9 ± 0.2 2.5 ± 0.3 7 1.8 ± 0.2 2.3 ± 0.3

In these groups of animals, the index values decrease, and in the group of cows with the first degree of endotoxemia they return to normal.

Example 1. Sick cow, age 5 years, red steppe breed. Diagnosis: acute postpartum catarrhal-purulent endometritis. The animal was oppressed. There was a deterioration in general condition, a decrease in appetite, and a decrease in milk productivity. Subfebrile fever of a constant type was observed, a slight increase in pulse and respiration, and increased body temperature. During lying down, the act of defecation, urination, as well as during rectal massage of the uterus, a thick, cloudy grayish-colored exudate with an admixture of pus was released from the cervical canal. During rectal examination, an increase in the uterus was noted, asymmetry - the right horn is much larger than the left. According to clinical and laboratory research - endogenous intoxication: the amount of hemoglobin - 85 g / l; erythrocytes - 5.1 · 10 ¹² / l; white blood cells - 6.93 · 10 ⁹ / l; the content of albumin is 22.3 g / l, the content of VNSSM plasma is 11.9 srvc. units ² ; the content of VNSMM of erythrocytes is 20.1 srvc. unit ² . The value of AI is 2.6.

According to the totality of clinical data, the state of moderate severity, according to the value of AI - the first degree of endotoxemia. Comprehensive treatment was aimed at raising the general tone of the body, increasing the contractility of the uterus, removing exudate from its cavity, creating antiseptic conditions in the uterus. Conducted therapy led to a complete recovery on the 7th day, the value of AI became equal to 1.9.

Example 2. Sick cow, age 4 years, red steppe breed. Diagnosis: acute postpartum catarrhal-purulent endometritis. The animal was depressed, groaning, bending its back. Rapid breathing, fever, fever, decreased milk secretion were noted, and a mucopurulent exudate from the external genitalia exuded a viscous consistency of fetid odor of a dull yellowish and brown color. A rectal examination revealed an increase in both uterine horns. According to clinical and laboratory studies - endogenous intoxication: the amount of hemoglobin - 63 g / l, red blood cells - 3.7 · 10 ¹² / l; white blood cells - 4.1 · 10 ⁹ / l; an increase in certain types of white blood cells; ESR according to the Panchenkov method - after 1 hour; the content of albumin is 20.1 g / l; VNSSM plasma content - 19.7 srvc. units ² ; the content of VNSMM of erythrocytes is 26.3 srvc. unit ² . The value of AI is 3.7.

According to the set of clinical data of special gynecological studies, the general condition is recognized as serious. According to the value of AI - the 2nd degree of endotoxemia, the threat of sepsis. Conducted a comprehensive treatment. To suppress the development of pathogenic microflora in the uterus, local and general antiseptic therapy has been prescribed, taking into account the sensitivity of the isolated microbes to the drug used. Conducted therapy led to recovery on the tenth day. The value of AI has become equal to 2.1.

Claims (1)

A method for diagnosing the degree of endotoxemia in cows with acute purulent-catarrhal endometritis, characterized in that they determine the content of substances of low and medium molecular weight in plasma and red blood cells, the total concentration of plasma albumin, calculate the integral index (II) based on the obtained parameters by the formula AI = 100 · S _{238-298 (plasma)} / S _{238-298 (red blood cells)} · OKA, where S _{238-298 (plasma)} , S _{238-298 (erythrocytes)} are the content of substances of low and medium molecular weight of plasma and red blood cells, respectively, determined by the area of the figures bounded by spectral curves in the wavelength range of 238-298 nm and the abscissa ( conditional unit ² ); OKA - total concentration of plasma albumin (g / l), and with its value from 2.1 to 3.0, the first degree of endotoxemia is diagnosed, from 3.1 to 4.5 - the second, from 4.6 to 6.0 - the third degree, above 6.0 - the fourth degree of endotoxemia, with normal from 0.5 to 2.0.