RU2198664C2 - Method for suppressing hiv-infection - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: one should perform ultrasound sanitation of emptied rectum. Then comes gradual ultrasound impregnation of ozonized physiological solution or ozonized fatty emulsion. It is necessary to conduct ultrasound contact rectal treatment. Then one should carry out endovaginal, and in men - urethral impregnation of ultrasound aerosol of medicinal preparation into mucous tissues followed by aeration with either ozone-oxygen or ozone-air gaseous mixtures. As ozonized physiological solution one should use 0.9%- sodium chloride solution or 5%-glucose solution. Ozone concentration in solution corresponds to 1-10 mg/l. Ozone concentration in ozone-oxygen and ozone-air gaseous mixture is 1-20 mg/l. Peroxide number P of ozonized fatty emulsion of "oil-in-water" type is about 500, not lower. The present method enables to maintain body ozone dosage for the period exceeding, at least, one HIV replication cycle. EFFECT: higher efficiency. 7 cl, 12 dwg, 2 ex

Description

 The invention relates to medicine, namely to methods of suppressing HIV infection that causes AIDS in humans, and can be used for the prevention and treatment of AIDS.

 AIDS is the first viral infection caused by the human immunodeficiency virus (HIV), the spread of which (since 1980) is characterized not by local foci, but by a pandemic, which by 1999 had spread to almost all countries of the world.

 According to forecasts by WHO and a group of independent experts, by 2000 the number of people infected with HIV, i.e. people, probably doomed to die of AIDS, will reach, according to various estimates, from 20 to 110 million, and the number of deaths from AIDS will exceed 8 million [1].

 The increasing incidence and mortality from AIDS in the absence, to date, of effective drugs and methods for its treatment and prevention, require an intensification of the search for new drugs and the development of new medical technologies to combat HIV infection.

 Known methods of suppressing HIV infection, which include introducing nucleotide analogues into the body, in particular 3-azi-3-deoxythymidine-AZT (AZT), as well as defluorinated nucleosides, liposome nucleoside analogues, etc. [1, 2].

 However, the effectiveness of the methods used to suppress HIV infection is low, because the drugs used in the treatment only slightly extend the life of many patients and are effective only in the early stages of the development of the disease. Some of them (for example, the most effective drug AZT) even at therapeutic doses have high toxicity, giving complications in clinical practice that limit their use. In addition, most drugs, due to the powerful blood-brain barrier, which provides effective protection of brain cells from foreign chemical substances circulating in the blood, are not able to penetrate the brain tissue, cells of which are often infected by the HIV virus.

 Without the creation of a slightly toxic biocompatible drug used for a considerable time (weeks, months, and years without concomitant toxic reactions), commensurate with the time of replication of the HIV virus, which can be present for a long time and circulate in the body of an infected AIDS patient, penetrate like brain cells and in other tissue cells of the body, the solution to the AIDS problem is impossible.

 A known method of suppressing HIV infection is the introduction into the body by intravascular autohemotransfusion of photomodified blood (AUFC) [3]. It was shown [3, 4] that the positive effect in the treatment of AIDS using AUFOK (UV irradiation of the patient’s autologous blood in a flowing quartz cuvette stimulating the synthesis of biologically active substances, including traces of ozone from oxygen dissolved in blood plasma) can be associated with the activation of oxidative recovery processes that have a positive effect on the hemostatic system, as well as on indicators of cellular immunity.

 However, despite the fact that subsequent serological studies (after 2 sessions of AUFC) did not reveal HIV-specific antibodies in the patient, the clinical and epidemiological diagnosis of AIDS was not in doubt. This indicates an insufficient manifestation of the effectiveness of this method, which may be due to the time-limited and the cyclical effect of the treatment factor on HIV, which has, as a rule, a long and aperiodic (depending on the state of the patient’s immune system) replication cycle (weeks months). In addition, UV radiation, intensively absorbed in the surface layers of the blood (0.05-0.1 mm), sharply reduces its effect on biosystems, and hence the initiation of physicochemical and biological processes that have a virucidal effect. There is evidence [5] that HIV is relatively resistant to UV radiation and ionizing radiation.

 Closest to the claimed technical solution is a method of suppressing infection, including intravenous administration of an ozonized substance into the body [6]. The concentration of ozone in the dosage form is from 1-10 mg / l. The number of sessions is up to 20-25, conducted both daily and periodically 1-2 times a week. The effect of the application of the method is manifested both through the direct effect of ozone on the virus, and indirectly - through the action of peroxides on the virus resulting from the interaction of ozone with the biological structures of the body and the immunomodulatory effect of ozone. At the same time, ozone stimulates phagocytosis, has a protective effect on healthy cells, while at the same time increases the elimination of cells affected by the virus.

The capabilities of the prototype method in relation to suppressing infection in hepatitis having similar transmission routes as HIV infection, on the one hand, indicate the validity of using ozone to inactivate HIV infection, and on the other, the impossibility of using it to effectively suppress HIV AIDS infections for the following reasons:
- the duration of the incubation period and the aperiodicity of HIV replication (days, weeks, months, depending on the state of the immune system of an infected person), incompatible with the short period of the active state of ozone (ozone deactivation time - of the order of several seconds) introduced into the human body;
- the production of a large number of biologically active peroxide compounds formed during the rapid decomposition of ozone directly from a blood and lymph substrate, leads to a sharp removal of substances necessary for their normal functioning from these substrates, which necessitates limiting the amount and concentration of ozone introduced into the body;
- the short duration of the period in the blood and lymph systems of sufficient quantity and concentration of ozone and its derivatives (ozonides, peroxides, etc.), quickly consumed due to its good solubility and high reactivity, as well as absorbed by the reticuloendothelial system and liver, does not allow to achieve reliable suppression of HIV after inactivation and exhaustion of their virucidal properties;
- the inability to create a long-term depot of a biocompatible virucidal substance based on gaseous ozone or ozonized physiological solution (0.9% NaCl, etc.) and derivatives of ozone (ozonides, peroxides, etc.) circulating in the blood and lymph systems and constantly interacting with migrating and concentrating in internal environments and in tissues of various levels of organization by leukocytes and macrophages infected with HIV;
- the inability to exclude the reinfection of susceptible cells of the body with viral particles of HIV, which is an intracellular parasite, aperiodically emerging from already infected cells in the process of continuous HIV replication.

 The objective of the invention is to increase the effectiveness of inhibiting HIV infection by creating a biocompatible with the body virucidal drug that maintains and maintains its antiviral activity for a long time in therapeutically adequate concentrations for a period exceeding at least one cycle of possible HIV replication.

 The problem can be solved due to the fact that according to the method of suppressing HIV infection, including intravenous administration of an ozonized substance into the body, physiological saline and oil-in-water fat emulsion are used as the ozonized substance, ultrasonic sanitation of the empty rectum is performed, and then, ultrasonic impregnation of ozonized physiological saline or ozonized fat emulsion into it and contact ultrasonic processing of the rectum are carried out successively; in women, endovaginal, and in men, urethral impregnation in the mucosal tissue with ultrasonic or pneumomechanical aerosols of these drugs is carried out, followed by aeration with ozone-oxygen or ozone-air mixtures.

 Moreover, an ozonized oil-in-water fat emulsion is a fat emulsion with a peroxide number P of at least 500, obtained by ultrasonic aeration of the fat emulsion for parenteral administration by bubbling through it an ozone-containing gas component in the form of an ozone-oxygen or ozone-air gas mixture.

 At the same time, a 0.9% physiological solution of sodium chloride is used as the ozonized physiological solution with an ozone content of 1-10 mg / l, obtained by ultrasonic aeration while bubbling through it an ozone-containing gas component in the form of an ozone-oxygen or ozone-air gas mixture.

 At the same time, a 5% physiological glucose solution is used as the ozonized physiological solution with an ozone content of 1-10 mg / l, obtained by ultrasonic aeration while bubbling through it an ozone-containing gas component in the form of an ozone-oxygen or ozone-air gas mixture.

 In this case, the aerosol of an ozonized oil-in-water oily emulsion or ozonized physiological saline is a finely divided aerosol of the corresponding liquid obtained by spraying it in a low-frequency range ultrasound field.

The aerosol of ozonized fat emulsion of the oil-in-water type is a fine aerosol obtained by pneumomechanical spraying using a composition in the following ratio, wt. %:
Ozonized soybean oil with peroxide number P- not more than 500 - 37.5
Refined soya lecithin (phospholipids) - 1.2
Glycerin - 2.5
Distillate or propylene rest - Up to 100.0
In addition, aeration of the urethra, vagina and rectum with an ozone-oxygen or ozone-air gas mixture is carried out at an ozone concentration of 10-20 mg / l.

 A feature of the proposed method of suppressing HIV infection is the creation of long-term contact of a highly active virucidal drug substance biologically compatible with body tissues based on a colloidal carrier (oil emulsion) containing ozone derivatives (ozonides, peroxides, etc.) that maximally saturate blood and lymph , and through them all the tissues of the body and, as a result, everywhere interacting with HIV, causing its inactivation. In this case, the property of vegetable oils (soybean, olive, sunflower, etc.) is used to intensively, in very large quantities, chemisorb ozone and create a long-term depot of ozonides and peroxides in its volume due to the interaction of ozone with double C = C bonds of unsaturated carboxylic acids included in composition of oil triglycerides. The resulting and additionally accumulated colloidal carrier - oil, ozonides and peroxides, which are the active components of the used oil-in-water ozonized fat emulsion and are introduced into the body’s tissues in various ways and in different phase states, contribute to blocking the reproduction of HIV by creating biological body fluids and tissues of an environment that deactivates HIV outside the cell, when HIV spreads from cell to cell, inhibiting the interaction of the HIV gp 120 region with the CD4 cell receptor on the surface of membranes of various leukocytes and macrophages, as well as having an inhibitory effect on the process of HIV replication in cells of blood cells.

The invention is illustrated by drawings, which depict
figure 1 - ultrasonic chamber to obtain a homogeneous ozone-containing liquid mixtures;
figure 2 - scheme of intravenous drip of ozonized fat emulsion of the type oil-in-water or ozonized physiological saline;
figure 3 - diagram of rectal ultrasonic sanitation of the rectum and subsequent ultrasonic impregnation in the blood and lymph system of ozonized fat emulsion of the type oil-in-water or ozonized physiological solution;
in FIG. 4 is a diagram of rectal ultrasonic impregnation of an ozonized oil-in-water type oily emulsion or ozonized physiological saline solution with a contact ultrasound into the blood and lymph system;
5 is a diagram of a rectal ultrasonic gas-liquid treatment of the rectal mucosa with an aerosol torch of an ozonized oil-in-water oily emulsion or ozonized physiological saline solution when they are impregnated into the blood and lymph system;
in FIG. 6 - node 1 in figure 5 (section of the working end of the waveguide tool with an internal axial channel and a developed conical radiating end face);
Fig.7 is a diagram of the urethral ultrasonic sanitation of the urethra and subsequent ultrasonic impregnation in the blood and lymph system of an ozonized fat emulsion of the type oil-in-water or ozonized physiological saline;
on Fig - diagram of endovaginal ultrasonic sanitation of the vagina and subsequent ultrasonic impregnation in the blood and lymph system of ozonized fat emulsions of the type "oil in water" or ozonized physiological solution;
Fig.9 is a diagram of an endovaginal ultrasonic gas-liquid treatment of the vaginal mucosa with an aerosol torch of an ozonized oil-in-water oily emulsion or ozonized saline solution when they are impregnated into the blood and lymph system;
figure 10 is a General view of a device for pneumomechanical spraying of ozonized fat emulsions of the oil-in-water type or ozonized physiological saline in the form of an aerosol plume;
figure 11 is a diagram of the stage of introduction of a device for pneumomechanical spraying ozone-containing gas-liquid components into the vagina;
in FIG. 12 - node 11 in Fig. 11 (exposure to an aerosol torch of an ozonized oil-in-water oil emulsion or ozonized saline solution on the vaginal mucosa when they are impregnated into the blood and lymph systems.

 The following devices are used to implement the method of suppressing HIV infection: device I - for creating ozone-containing drugs in different phase states (liquid, gas-liquid and gaseous) and introducing them into the body of an HIV-infected person using low-frequency ultrasound energy and device II - for introducing ozone-containing drug substance in the gas-liquid phase (aerosol) into the body of an HIV-infected person by pneumomechanical spraying.

Device I contains the following main blocks:
- a unit for preparing an ozone-containing drug substance in a liquid phase state 1.1 (Fig. 1), including: an ozone generator (GO) 1.1.1, an ultrasonic generator (USG) 1.1.2, an ultrasonic camera (US camera) 1.1.3. In this case, the ultrasonic camera 1.1.3 contains a supporting body 1.1.3.1 with a removable cover 1.1.3.2, forming a sealed container 1.1.3.3, in the bottom of which a piezoceramic ultrasonic transducer 1.1.3.4 is installed, coupled to a radiating waveguide-activator 1.1.3.5 connected in turn with an ultrasonic generator (USG) 1.1.2. A nozzle 1.1.3.6 was made into the tank 1.1.3.3 for supplying the gas component to the ultrasonic chamber 1.1.3 from the ozone generator (GO) 1.1.1 through the pneumatic pipe 1.1.3.7 connected to the annular atomizer-nozzle 1.1.3.8 mounted coaxially to the emitting waveguide -activator 1.1.3.5 (for directed bubbling of a sprayed jet of an ozone-containing gas component into the developed cavitation region created in the field of powerful ultrasound by an emitting waveguide-activator 1.1.3.5). A discharge fitting is connected to the bottom of the sealed container 1.1.3.3, communicating it through a control and shutoff valve 1.1.3.9 with a distribution hydraulic line 1.1.3.10. In addition, on a removable cover 1.1.3.2, a decontaminant of excess ozone 1.1.3.11 is fixed, which communicates with the capacity 1.1.3.3;
- block intravenous drip of ozone-containing medicinal substance in the liquid phase state 1.2 (figure 2), which includes a measured capacity (bottle) 1.2.1 filled with a solution of ozonized fat emulsion of the type "oil in water" or ozonized physiological solution, as well as standard a disposable system for blood transfusion 1.2.2 with a regulator of supply of a solution 1.2.3;
- block ultrasonic sanitation of body cavities and impregnation of an ozone-containing medicinal substance in liquid or gas-liquid phase states in biological tissue, blood and lymph system of an organism infected with HIV 1.3 (Fig. 3, 4, 5, 7, 8 and 9), including an ozone generator (GO) 1.1.1 (not shown in the diagram), an ultrasonic generator (UZG) 1.3.1, an acoustic unit 1.3.2 with an attached waveguide-tool, the design and purpose of which are determined by one or another stage of ultrasonic sanitation of the body cavities and infected with HIV, or in the impregnation of the biological tissue, blood and lymph organism ozone-containing drug. So, at the stage of rehabilitation of body cavities through an intermediate solution of ozone-containing medicinal substance, a cylindrical waveguide tool 1.3.3 is used with a radiating end beveled at an angle of 45 ^o (Fig. 3, Fig. 7 and Fig. 8). At the stage of cavity contact ultrasonic impregnation of an ozone-containing medicinal substance, a waveguide tool 1.3.4 is used with a perforated hollow olive-shaped radiating working end 1.3.5 (Fig. 4), into the cavity of which a capillary-porous element 1.3.6 is impregnated with an ozone-containing drug solution, and at the stage of gas-liquid treatment of body cavities with an aerosol of ozone-containing medicinal substance, a waveguide tool 1.3.7 (Fig. 3, Fig. 6 and Fig. 9) equipped with an internal axial channel 1.3.8 (with p zvitym tapered end radiating 1.3.9) for feeding through the channel 1.3.8 ozone-containing solution of drug development cavitation that occurs near the emitting end face. At the same time, the waveguide tool 1.3.3, used at the stage of rectal ultrasonic rectification of the rectum through intermediate ozone-containing medicinal substances (ozonized fat emulsion of the oil-in-water type or ozonized physiological saline solution) and their subsequent impregnation into the blood and lymph system (Fig. 3 ), is connected through a guide fluoroplastic sleeve 1.3.10 (eliminates the "spreading" of ultrasound directly onto the patient’s body outside the working area) with a rectal perforated extender-limiter 1.3.11 and has the ability sevogo and circular movement in the internal cavity of the expander limiter 1.3.11. In the expander-limiter 1.3.11 along the generatrix in contact with the cavity of the rectum, perforations 1.3.12 are made, which provide the most complete contact and exposure of the rectal mucosa of medicinal substances and ultrasound, as well as the supply pipe 1.3.13, providing an intermediate supply ozone-containing medicinal substances (ozonized fatty emulsion of the oil-in-water type or ozonized physiological saline solution) into the cavity of the expander-limiter 1.3.11 from the associated main supply Box 1.3.14, which in turn is associated with an ozone-containing drug preparation unit in a liquid phase state 1.1 or an ozone-containing drug substance preparation intravenous block in a liquid phase state 1.2.

 Similar to the above rectal dilator-limiter 1.3.11 (in design and functional purpose) for urethral ultrasonic sanitation of the urethra and subsequent ultrasonic impregnation into its mucous membrane and blood and lymph system of an oil-in-water ozonized fat emulsion or ozonized saline solution is used (Fig. 7) a perforated extender-limiter 1.3.15, equipped with a guide fluoroplastic sleeve 1.3.16, coupled to the waveguide tool 1.3.3, as well as the supply and outlet pipe 1 .3.17, connected in turn through a main supply pipe 1.3.14 with an ozone-containing medicinal substance preparation unit in the liquid phase state 1.1 or an ozone-containing medicinal substance intravenous drip administration unit in the liquid phase state 1.2.

 For the implementation of endovaginal ultrasonic sanitation of the vagina through an intermediate solution of an ozone-containing medicinal substance (ozonized fat emulsion of the oil-in-water type or ozonized physiological saline solution) and its subsequent impregnation into the mucous membrane and blood and lymph system (Fig. 8), a trellised (perforated) vaginal sheath is used expander-limiter 1.3.18, equipped with a centering and guiding fluoroplastic sleeve 1.3.19, coupled to the waveguide tool 1.3.3, as well as the supply pipe 1.3.20 connected о in turn, through the main supply pipe 1.3.14 with an ozone-containing medicinal substance preparation unit in the liquid phase state 1.1 or an ozone-containing medicinal substance intravenous drip administration unit in the liquid phase state 1.2.

 At the stages of rectal (Fig. 5) or endovaginal (Fig. 9) ultrasonic gas-liquid treatment of the mucous membrane of the corresponding cavity with an aerosol torch of an ozonized oil-in-water oily emulsion or ozonized physiological saline solution (when they are impregnated into the mucosa and blood and lymph system), respectively, lattice (perforated) rectal dilator-limiter 1.3.21 and vaginal dilator-limiter 1.3.22, as well as acoustic nodes 1.3.2 and waveguide-instruments 1.3.7 with an attached main supply pa a tube 1.3.14, which in turn is connected to a unit for preparing an ozone-containing drug substance in a liquid phase state 1.1 or an intravenous drip unit for administering an ozone-containing drug substance in a liquid phase state 1.2.

 The device II, intended for the introduction of an ozone-containing medicinal substance in the gas-liquid phase (aerosol) into the body of an HIV-infected person by pneumomechanical spraying, contains a supporting body 2.1, an aerosol can 2.2 coupled through a spray head 2.3 with a trellised (perforated) vaginal dilator-limiter 2.4, which allows the introduction of device II into the vagina to fully expand it from the mouth to the arches with exposure of the vaginal part of the cervix.

 The method of suppressing HIV infection is carried out in a single, continuously ongoing technological cycle of exposure to the body of an HIV infected or AIDS patient as follows.

 Initially, according to generally accepted methods, a clinical and immunological diagnosis of the stage of HIV infection is carried out and treatment tactics are determined.

Preliminarily, in an ultrasonic chamber 1.1.3 (FIG. 1), a unit for preparing an ozone-containing drug in the liquid phase state 1.1 of device I, an ozonized oil-in-water oily emulsion (ozone-containing colloidal carrier) is prepared by creating a uniform metastable ozone-containing gas-liquid mixture, which differs a large peroxide number (of the order of 500-800 or more), which ensures the intensification of the processes of disintegration, dissolution and ozonation of liquids (water, oil, etc.) to obtain oil-in-water emulsions de "+ O ₃ in the field of powerful ultrasound, by sparging through it a gas component (ozone contained in a gas-air or oxygen mixture), carried out in an ultrasonic chamber.

Parameters of ultrasonic aeration mode
- frequency of ultrasonic vibrations - 44.0 kHz;
- the amplitude of the ultrasonic vibrations of the activator waveguide is 30-35 microns;
- exposure of ultrasonic exposure - 1-2 s / cm ³ sonicated fluid;
- consumption of ozone-containing gas component (ozone-air or ozone-oxygen mixture) - not less than 500 ml / min;
- the concentration of ozone supplied to the ultrasonic chamber 1.1.3, at a flow rate of an ozone-containing gas component (ozone-oxygen or ozone-air mixture) 500 ml / min - not less than 5 mg / l.

 Moreover, pronounced secondary factors of powerful ultrasound: cavitation, acoustic flows, variable sound pressure, etc., generate in the region of the activator waveguide 1.1.3.5 a whole field of oscillating cavitation bubbles interacting with bubbles of an ozone-containing gas component supplied to the developed cavitation region. This ensures their destruction into smaller nuclei, collapsing cavitation bubbles with the creation of a developed phase contact surface (gas-liquid). In addition, the intense acoustic flows, hydrodynamic disturbances, and cumulative jets at the macro and micro levels lead to the intensification of macro and micro mixing processes, which significantly reduce the thickness of the boundary diffusion layer at the phase boundary. This leads to an acceleration of external diffusion, a decrease in the concentration gradients of dissolved substances at the phase boundary, and acceleration of heterogeneous chemical reactions that determine the dynamics of absorption of free ozone-containing gas in the sounded liquid, i.e., the process of acoustic aeration of liquid ozone with the achievement of a metastable system - dispersion of ozone-containing gas gas component in a liquid. Studies on the acoustic aeration of ozone in solutions have shown that the concentration of ozone and its derivatives substantially depends on the type of solution (ozonized oil-in-water oily emulsion or ozonized physiological saline), on the type of ozone-containing gas component sparged into the solution, on the initial ozone concentration in it, as well as from the exposure of the bubbling of the ozone-containing gas component and the time of scoring.

 For example, when sounding the ozonized physiological solution and simultaneously bubbling through it an ozone-containing gas component (ozone-air or ozone-oxygen mixture), a sufficient concentration of ozone in the ozonized physiological solution is achieved in 1-3 minutes, while the same process, but without sounding, allows receive the same concentration of ozone in ozonized physiological saline solution only after 15-20 minutes.

At the same time, acoustic aeration of oil-in-water type fat emulsions revealed the following peculiarity of their ozonation, associated with the intense saturation of their ozone in the ultrasound field, which is approximately an order of magnitude greater than the saturation of the emulsion without sounding it. This is due to the fact that during ozonation of an oil-in-water fat emulsion with an ozone-containing gas component (ozone-air or ozone-oxygen mixture), under conditions of developed cavitation, the microparticles of the oil undergo powerful kinetic and hydromechanical influences, as a result of which they constantly change shape - they are deformed and burst by folding cavitation bubbles and acoustic currents into smaller particles, followed by coalescence. The formation of ozonides and peroxides of oil triglycerides occurring on the surface of these microparticles proceeds under special conditions of the kinetics of mass transfer processes (due to the effects of ultrasonic exposure): intensification of mixing in the thickness of the aqueous phase, reduction of the boundary diffusion layer from the aqueous phase, intensive mixing inside the microparticles of oil, microflows in the thickness of the oil phase and in the boundary diffusion layer induced by ultrasonic vibrations, etc. This ensures a uniform distribution of ozonides and peroxides throughout the volume of the microparticle (micelle) of the oil, which allows creating in it, and not just on its surface, a significant depot of active components that gradually diffuse into the aqueous phase, and when in the patient’s body, into the blood and lymph . This ensures a long therapeutic effect. The recommended peroxide number P of emulsified oil is not less than 500. P is a measure of the active oxygen content in peroxide form - the amount of mmol O _act per 1 kg of oil, i.e. characterizing the bactericidal and virucidal potential of an ozonized oil-in-water fat emulsion in the process of suppressing HIV infection.

Since fat emulsions are widely used in medical practice for parenteral nutrition [7, 8], to obtain a biocompatible ozonized fat emulsion of the oil-in-water type, the following composition of starting components allowed in pharmacy was used, in the following ratio, wt.%:
Ozonized soybean oil with peroxide number P not more than 500 - 10.0
Refined soya lecithin (phospholipids) - 1.2
Glycerin - 2.5
Distillate or water for injection else - Up to 100.0
A homogeneous ozonized physiological solution is obtained in the same way at the above acoustic and technological parameters by ultrasonic aeration while bubbling the ozone-containing gas component through a 0.9% physiological solution of sodium chloride or 5% glucose solution, with an ozone content of 1-10 mg / l.

 After obtaining stable and uniform ozone-containing medicinal substances according to the above procedure, an ozone-containing oil-in-water oily emulsion and an ozone-containing physiological saline, depending on individual tolerance, in the first stage of HIV suppression and AIDS treatment (Fig. 2), first for 10 -15 procedures carried out daily using the block of intravenous drip administration of ozone-containing medicinal substance in the liquid phase state 1.2, carry out intravenous drip administration of ozone-containing physiological saline solution, and then instead of it, at least 10 additional intravenous drip administrations of an oil-in-water ozone-containing fat emulsion are carried out every other day.

 At the same time, the next second stage of HIV suppression by complex saturation of tissues and the internal environment of an HIV-infected or AIDS patient is carried out every other day or in the intervals between intravenous drips of an ozone-containing oil-in-water fat emulsion. For this, in the Trendelenburg position, the patient after preliminary rectal ultrasonic sanitation of the empty rectum through an intermediate antiseptic solution (for example, furatsilin 1: 5000, 2% hydrogen peroxide, etc.) is carried out sequentially as a rectal ultrasonic impregnation of an ozone-containing oil-in-oil emulsion of the type “oil in water "or ozonized saline solution (figure 3), and contact ultrasound treatment of the rectum (figure 4).

Parameters of the regime of ultrasonic sanitation through intermediate solutions of antiseptics or ozone-containing medicinal solutions
- frequency of ultrasonic vibrations - 26.5 kHz;
- the amplitude of the ultrasonic vibrations of the waveguide tool 1.3.3 with a radiating end beveled at an angle of 45 ^o - 50-60 microns;
- exposure of ultrasonic exposure - 1-2 s / cm ³ solution filling the voiced cavity;
- the consumption of an antiseptic solution or ozone-containing medicinal substance (ozone-containing fatty emulsion of the oil-in-water type or ozonized physiological saline solution) - not more than 120 ml / min;
- peroxide number P of ozone-containing fatty emulsion of the type "oil in water" - not less than 500;
ozone concentration in ozonized physiological saline is 1-10 mg / l.

Parameters of the contact ultrasonic treatment of the rectum through intermediate ozone-containing medicinal solutions (ozonized oil-in-water oily emulsion or ozonized physiological saline
- frequency of ultrasonic vibrations - 26.5 kHz;
- the amplitude of the ultrasonic vibrations of the waveguide tool 1.3.4 with a perforated hollow olive-shaped radiating working end - 30-35 microns;
- exposure of ultrasonic exposure - 20-30 s;
- peroxide number P of ozone-containing fatty emulsion of the type "oil in water" - not less than 500;
- the concentration of ozone in ozonized physiological saline is 1-10 mg / l.

 Further, depending on the patient’s gender, urethral (in men) (Fig. 5) or endovaginal (in women) (Fig. 8) ultrasound impregnation into the mucosal tissue is performed by voicing them through an intermediate solution of ozone-containing medicinal substance using an ultrasonic sanitation unit of the body cavities and impregnation of an ozone-containing drug substance in liquid or gas-liquid phase states in biological tissue, blood and lymph systems of an organism infected with HIV 1.3.

 At the third stage of the process of suppressing HIV or treating the developed AIDS, initially rectal in men (Fig. 5), rectal and endovaginal in women (Fig. 5 and Fig. 9) are daily impregnated with an ultrasonic or pneumomechanical aerosol of ozonized oil emulsion fat emulsion water "or ozonized physiological solution of the mucous membrane of the indicated body cavities, and then their subsequent aeration with ozone-air or ozone-oxygen mixtures at an ozone concentration of 10-20 mg / l, which is optimal.

In this case, the impregnation of a finely dispersed aerosol of ozonized oil-in-water oily emulsion or ozonized physiological saline is carried out in the low-frequency range ultrasound field with the parameters of the ultrasonic atomization mode
- frequency of ultrasonic vibrations - 26.5 kHz;
- the amplitude of the ultrasonic vibrations of the waveguide tool 1.3.7, equipped with an internal axial channel is 70-80 microns.

When impregnating a finely divided aerosol of ozonized oil-in-water oily emulsion by pneumomechanical spraying, the composition of the emulsion is used in the following ratio, wt.%:
Ozonized soybean oil with peroxide number P not more than 500 - 37.5
Refined soya lecithin (phospholipids) - 1.2
Glycerin - 2.5
Distillate or propylene rest - Up to 100.0
In an in vitro experiment, when exposed to ozone on blood cells, it was found that when the ozone concentration is less than 2 mg / l, it does not have a damaging effect on them, nor does it have a pronounced inhibitory effect on HIV infection (according to [ 10]), and at a concentration of more than 20 mg / l, the first signs of the damaging effects of ozone on the blood formed elements are possible.

 The rationale for the effectiveness of the proposed method of suppressing HIV infection is the data of studies conducted in the Research and Production Association "Granite" Komshalyukom MS et al. [9] on the assessment of the tolerance of ozone therapy for patients with chronic hepatitis of viral etiology (Example 1), as well as studies conducted at the Institute of Virology named after DI. Ivanovsky Kornilayeva G.V. et al. [10] to assess the effectiveness of the effects of ozone on HIV infection (Example 2).

 Example 1. Ozone therapy of viral hepatitis was used in the treatment of 35 patients aged 32 to 58 years (mean age 41 years). Of these: men - 25, women - 10. Markers of hepatitis B were diagnosed in 21 patients, anti-HCV were detected in 14 patients, mixed infection in 5 cases.

 Ozone therapy was used every other day in the form of an intravenous drip of an ozonized isotonic solution of 0.9%, 400 ml each, with an ozone concentration of 3 mg / L. The course of treatment is 10-12 procedures. In 15 cases, ozone therapy was used as monotherapy. In the course of treatment, ozone therapy was well tolerated by all patients. Side effects were not observed. Positive dynamics was traced in the clinical picture of the disease: all patients had asthenic syndrome, pain in the right hypochondrium, dyspeptic symptoms, itching, jaundice decreased. Changes in blood biochemical parameters - a significant decrease in the level of bilirubin, aminoferases, alkaline phosphatase, gammaglutamine transpeptidase, correlated with the clinical symptoms of the disease. In 9 patients out of 35, after 5 procedures, there was a slight increase in aminoferases and bilirubin with a decrease in these indicators after 10 ozone therapy procedures, which may be associated with an increase in the activity of the process in the liver. Patients received a positive clinical result of treatment.

 Example 2. The process of inactivation of HIV infection in vitro was simulated on cultures of chronically infected HIV cell lines — CEM / LAV I BRU and EVK / IRA 3, and cell lines sensitive to infection with this virus — CEM and U 937. Viral antigen was detected with using enzyme immunoassay and indirect immunofluorescence.

 It was shown that ozone in a concentration of from 0.4 to 2 mg / l (extremely low concentrations of ozone) does not have a pronounced inhibitory effect on HIV infection in a chronically infected culture, although it significantly enhances the proliferative activity of cells. Starting at a concentration of 4 mg / l, ozone transforms the chronic type of infection into a lytic one, resulting in cell death. Most noteworthy is the fact that chronically infected cells treated with 10 mg / l ozone lose their ability to initiate infection when they are co-cultivated with non-infected sensitive cells. When exposed to the same concentration of ozone on an extracellular virus located in a growth medium with 15% calf serum, its activity can be reduced by 33%. Complete deactivation of the extracellular virus is achieved by passing gaseous ozone through the virus-containing fluid.

 Thus, it was shown that the ozonation of an infected culture allows not only inactivating the extracellular virus, but also prevents its further production by infected cells, and, in addition, to destroy them.

 This property, acquired in the process of ozonation, with direct contact of ozone-containing components with HIV infection in the body, implemented in the proposed method of suppressing HIV infection, creates real prerequisites for the complete suppression of HIV infection, as well as the prevention and treatment of AIDS patients.

 To date, exploratory and technological studies have been carried out on experimental clinical models of HIV-infected biological objects to substantiate and select ozone-containing dosage forms based on the use of environmentally friendly and biocompatible ozone-containing components in different phase states. Ultrasound devices, instruments and devices for the implementation of the "Method for suppressing HIV infection" were developed and put into serial production (ultrasound apparatus PROKTON, ultrasound apparatus GINETON, ultrasound apparatus STOMATON, ultrasound apparatus TONZILLOR, UZ -installation "ROSSONIK", apparatus for gas ozone therapy "OZOTRON", etc.

 Technological schemes and methods for ozone-ultrasonic treatment of HIV-infected recipients and AIDS patients have been developed, and technological and acoustic parameters and modes of ozone-ultrasound exposure to biological tissues have been optimized.

 The claimed invention will accelerate the improvement, development and implementation (upon receipt in the prescribed manner of the relevant permits of the Ministry of Health of the Russian Federation) of new bio- and medical technologies, methods and methods of preventing and treating with its help the most dangerous viral infections, chronoseptic and immunodeficiency states, tumor formations, AIDS and etc.

Sources of information taken into account
1. Pokrovsky VV Epidemiology and prevention of HIV and AIDS. - M .: Medicine, 1996 .-- 248 p.

 2. Manvelova M.A. et al. AIDS (review of patent documentation). - M., 1990. - 17 p. - Dep. in the All-Union Scientific Research Institute of the USSR 19190.

 3. Dvaladze N.A. and others. The positive clinical effect of autohemotransfusion of photomodified blood in the treatment of an AIDS patient. // Vestn. chir. - 1990. - 1. - P.55-57.

 4. Mechanisms of the effect of blood irradiated by UV rays on the human and animal body / Ed. E.I. Ganelina and K.A. Samoilova. - L .: Nauka, 1986 .-- 264s.

 5. Tishchenko L.D. and other AIDS: problems of treatment and prevention: Textbook. allowance. - M .: Publishing House of UDN, 1989. - S.7-9.

 6. Maksimov V. A. et al. The use of ozone in medicine. Literature review. // Express information. Series "Therapy". - M., 1993, Iss. 7 - 12. - S.1-22.

 7. Mashkovsky M.D. Medicines T.2. - Publishing house 13th. - Kharkov: Torsing, 1997 .-- 592 p.

 8. Medicines of foreign firms in Russia: a Handbook. - M.: AstraPharmService, 1993 .-- 720 p.

 9. Komshalyuk M. S. et al. Ozone therapy in the treatment of chronic liver diseases. // Hepatitis B, C, D and G - problems of study, diagnosis, treatment and prevention (Abstracts of the report. II Russian scientific and practical conference with international participation October 14-16, 1997). - M., 1997. - P.101-102.

 10. Kornilayeva G.V. et al. The effect of ozone on HIV infection in vitro. // Ozone in biology and medicine (Thesis. Report. I All-Russian Scientific and Practical Conf. June 25-26, 1992). - N. Novgorod, 1992 .-- S.23-24.

Claims (3)

 1. A method of suppressing HIV infection, including intravenous administration of an ozonized substance into the body, characterized in that physiological saline and oil-in-water fat emulsion are used as the ozonized substance, ultrasonic sanitation of the empty rectum is carried out, and then ultrasonic impregnation is carried out sequentially it contains ozonized saline or ozonized fat emulsion and contact ultrasound treatment of the rectum; they perform rectal, endovaginal, and in men urethral impregnation of these drugs with ultrasonic or pneumomechanical aerosols in the mucosal tissue, followed by aeration of ozone-oxygen or ozone-air gas mixtures. 2. The method according to claim 1, characterized in that the oil-in-water ozonized fat emulsion is a fat emulsion with a peroxide number P of at least 500, obtained by ultrasonic aeration of the fat emulsion for parenteral administration by bubbling an ozone-containing gas component through it into as ozone-oxygen or ozone-air gas mixture
3. The method according to claim 1, characterized in that a 0.9% physiological solution of sodium chloride is used as the ozonized physiological solution with an ozone content of 1-10 mg / l, obtained by ultrasonic aeration while bubbling through it an ozone-containing gas component in as ozone-oxygen or ozone-air gas mixture
4. The method according to claim 1, characterized in that as the ozonized physiological solution, a 5% physiological glucose solution is used with an ozone content of 1-10 mg / l, obtained by ultrasonic aeration while bubbling through it an ozone-containing gas component in the form of ozone-oxygen or ozone gas mixture
5. The method according to claim 1, characterized in that the aerosol of ozonized fat emulsion of the type oil-in-water or ozonized physiological saline is a finely divided aerosol of the corresponding liquid obtained by spraying it in a low-frequency range ultrasound field. 6. The method according to claim 1, characterized in that the aerosol of ozonized fat emulsion of the oil-in-water type is a fine aerosol obtained by pneumomechanical spraying using a composition in the following ratio, wt.%:
Soybean oil, ozonized with a peroxide number P of not more than 500 - 37.5
Refined soya lecithin (phospholipids) - 1.2
Glycerin - 2.5
Distillate or propylene - Else up to 100.0
7. The method according to p. 1, characterized in that the aeration of the urethra, vagina and rectum with an ozone-oxygen or ozone-air gas mixture is carried out at an ozone concentration of 10-20 mg / l.

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