RU2017111331A - Ген и вариации, связанные с фенотипом bm1, молекулярные маркеры и их применение - Google Patents

Ген и вариации, связанные с фенотипом bm1, молекулярные маркеры и их применение Download PDF

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RU2017111331A
RU2017111331A RU2017111331A RU2017111331A RU2017111331A RU 2017111331 A RU2017111331 A RU 2017111331A RU 2017111331 A RU2017111331 A RU 2017111331A RU 2017111331 A RU2017111331 A RU 2017111331A RU 2017111331 A RU2017111331 A RU 2017111331A
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plant
gene
cad2
mutant
cad2 gene
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Вэй Чэнь
Натан Дж. ВАНОПДОРП
Сива П. КУМПАТЛА
Пэйчжун ЧЖЭН
Питер Д. ФРИДЕМАНН
Томас У. ГРИН
Деннис ФИТЦЛЬ
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Агридженетикс, Инк.
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Claims (13)

1. Способ переноса мутантного гена циннамилалкогольдегидрогеназы 2 (CAD2) кукурузы, включающий:
(a) анализ с применением зондов, которые способны к специфичной гибридизации по меньшей мере с одним маркером, который сцеплен с геном CAD2, геномной ДНК первого растения и геномной ДНК второго растения;
(b) половое скрещивание первого и второго растений с получением популяции потомства;
(c) анализ популяции потомства на присутствие по меньшей мере одного маркера, который сцеплен с геном CAD2;
(d) обратное скрещивание индивидуального растения из популяции потомства, которое включает по меньшей мере один маркер, который сцеплен с геном CAD2, с первым или вторым растением, с получением популяции следующего поколения;
(e) определение, включает ли представитель популяции следующего поколения требуемый признак от первого или второго растения и ген CAD2; и
(f) если ни один представитель популяции следующего поколения не включает требуемый признак от первого или второго растения и ген CAD2, повтор этапов (d) и (e), пока не будет идентифицировано индивидуальное растение, которое включает требуемые признаки от первого или второго растения и ген CAD2.
2. Способ по п.1, где индивидуальное растение потомства, полученное в каждом этапе скрещивания и обратного скрещивания, отбирают с помощью анализа маркера CAD2 в каждом поколении.
3. Способ введения мутантного гена циннамилалкогольдегидрогеназы 2 (CAD2) кукурузы в организм-хозяин с помощью генетической трансформации, где способ включает:
анализ геномной ДНК растения кукурузы с применением зондов, которые способны к специфичной гибридизации с маркерами, сцепленными с мутантным геном CAD2, для идентификации мутантного гена CAD2 в растении кукурузы;
выделение сегмента геномной ДНК растения кукурузы, который специфично гибридизуется с зондами, которые способны к специфичной гибридизации с маркерами, сцепленными с мутантным геном CAD2;
введение выделенного сегмента геномной ДНК в организм-хозяин; и
анализ ДНК организма-хозяина с применением зондов, которые способны к специфичной гибридизации с маркерами, сцепленными с мутантным геном CAD2, для идентификации мутантного гена CAD2 в организме-хозяине.
RU2017111331A 2011-01-03 2012-01-03 Ген и вариации, связанные с фенотипом bm1, молекулярные маркеры и их применение RU2017111331A (ru)

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CN110577960B (zh) * 2019-09-12 2022-09-13 南京农业大学 梨木质素合成基因PbMC1a/1b及其在果实品质遗传改良中的应用
CN110484647B (zh) * 2019-09-23 2020-08-07 齐鲁师范学院 与玉米细胞壁消化率相关的分子标记及其鉴别引物和应用
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CN112106651A (zh) * 2020-10-21 2020-12-22 山东省农业科学院作物研究所 一种bmr高粱杂交种的选育方法

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US20120174255A1 (en) 2012-07-05
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JP6101632B2 (ja) 2017-03-22
MX349339B (es) 2017-07-21
JP2014504864A (ja) 2014-02-27
WO2012094307A3 (en) 2013-03-14
UA117335C2 (uk) 2018-07-25
AR084764A1 (es) 2013-06-26
NZ612808A (en) 2015-06-26
WO2012094307A8 (en) 2012-10-18
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RU2017111331A3 (ru) 2021-01-12
WO2012094307A2 (en) 2012-07-12
RU2013136402A (ru) 2015-02-10
CN106148365A (zh) 2016-11-23
EP2661171A4 (en) 2014-09-17
AU2017206249A1 (en) 2017-08-10
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