RU2012127810A - Крупномасштабное получение функциональных мегакариоцитов и тромбоцитов из человеческих эмбриональных стволовых клеток в бесстромальных условиях - Google Patents
Крупномасштабное получение функциональных мегакариоцитов и тромбоцитов из человеческих эмбриональных стволовых клеток в бесстромальных условиях Download PDFInfo
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- RU2012127810A RU2012127810A RU2012127810/10A RU2012127810A RU2012127810A RU 2012127810 A RU2012127810 A RU 2012127810A RU 2012127810/10 A RU2012127810/10 A RU 2012127810/10A RU 2012127810 A RU2012127810 A RU 2012127810A RU 2012127810 A RU2012127810 A RU 2012127810A
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- Prior art keywords
- stem cells
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- pluripotent stem
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- 210000000130 stem cell Anatomy 0.000 title claims 2
- 238000011031 large-scale manufacturing process Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 66
- 210000003593 megakaryocyte Anatomy 0.000 claims abstract 26
- 210000001778 pluripotent stem cell Anatomy 0.000 claims abstract 21
- 210000003566 hemangioblast Anatomy 0.000 claims abstract 20
- 102000004127 Cytokines Human genes 0.000 claims abstract 14
- 108090000695 Cytokines Proteins 0.000 claims abstract 14
- 230000004069 differentiation Effects 0.000 claims abstract 14
- 239000003102 growth factor Substances 0.000 claims abstract 14
- 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 claims abstract 12
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 claims abstract 12
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims abstract 12
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims abstract 12
- 102100020715 Fms-related tyrosine kinase 3 ligand protein Human genes 0.000 claims abstract 9
- 101710162577 Fms-related tyrosine kinase 3 ligand protein Proteins 0.000 claims abstract 9
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims abstract 8
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims abstract 7
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims abstract 7
- 238000010494 dissociation reaction Methods 0.000 claims abstract 7
- 230000005593 dissociations Effects 0.000 claims abstract 7
- 238000000338 in vitro Methods 0.000 claims abstract 7
- 239000000126 substance Substances 0.000 claims abstract 7
- 102100020880 Kit ligand Human genes 0.000 claims abstract 6
- 101710177504 Kit ligand Proteins 0.000 claims abstract 6
- 102100027188 Thyroid peroxidase Human genes 0.000 claims abstract 6
- 101710113649 Thyroid peroxidase Proteins 0.000 claims abstract 6
- 235000015097 nutrients Nutrition 0.000 claims abstract 6
- 230000015572 biosynthetic process Effects 0.000 claims abstract 3
- 238000012258 culturing Methods 0.000 claims abstract 3
- 210000002242 embryoid body Anatomy 0.000 claims abstract 3
- 210000001671 embryonic stem cell Anatomy 0.000 claims abstract 2
- 239000002609 medium Substances 0.000 claims 29
- 102000003815 Interleukin-11 Human genes 0.000 claims 11
- 108090000177 Interleukin-11 Proteins 0.000 claims 11
- 210000001082 somatic cell Anatomy 0.000 claims 6
- 229920000669 heparin Polymers 0.000 claims 5
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims 5
- 230000024245 cell differentiation Effects 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims 2
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 claims 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims 2
- 102000026633 IL6 Human genes 0.000 claims 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims 2
- 229930003537 Vitamin B3 Natural products 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 229930182833 estradiol Natural products 0.000 claims 2
- 229960005309 estradiol Drugs 0.000 claims 2
- 210000003754 fetus Anatomy 0.000 claims 2
- 230000003394 haemopoietic effect Effects 0.000 claims 2
- 230000003343 megakaryocytopoietic effect Effects 0.000 claims 2
- 229960003512 nicotinic acid Drugs 0.000 claims 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims 2
- 230000003022 thrombocytopoietic effect Effects 0.000 claims 2
- 210000001519 tissue Anatomy 0.000 claims 2
- 239000011708 vitamin B3 Substances 0.000 claims 2
- 235000019160 vitamin B3 Nutrition 0.000 claims 2
- 102000007347 Apyrase Human genes 0.000 claims 1
- 108010007730 Apyrase Proteins 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0644—Platelets; Megakaryocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/145—Thrombopoietin [TPO]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/165—Vascular endothelial growth factor [VEGF]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2311—Interleukin-11 (IL-11)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
- C12N2501/91—Heparin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1394—Bone marrow stromal cells; whole marrow
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Diabetes (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26693909P | 2009-12-04 | 2009-12-04 | |
| US61/266,939 | 2009-12-04 | ||
| US38416510P | 2010-09-17 | 2010-09-17 | |
| US61/384,165 | 2010-09-17 | ||
| PCT/US2010/058990 WO2011069127A1 (en) | 2009-12-04 | 2010-12-03 | Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012127810A true RU2012127810A (ru) | 2014-01-10 |
Family
ID=44115332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012127810/10A RU2012127810A (ru) | 2009-12-04 | 2010-12-03 | Крупномасштабное получение функциональных мегакариоцитов и тромбоцитов из человеческих эмбриональных стволовых клеток в бесстромальных условиях |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9988603B2 (https=) |
| EP (1) | EP2507359A4 (https=) |
| JP (4) | JP2013512676A (https=) |
| KR (4) | KR20180086301A (https=) |
| AU (4) | AU2010325811A1 (https=) |
| BR (1) | BR112012013497A2 (https=) |
| CA (2) | CA2782013C (https=) |
| IN (1) | IN2012DN05054A (https=) |
| RU (1) | RU2012127810A (https=) |
| WO (1) | WO2011069127A1 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434527A (zh) | 2006-04-14 | 2017-02-22 | 安斯泰来再生医药协会 | 血管瘤集落形成细胞 |
| KR20180086301A (ko) | 2009-12-04 | 2018-07-30 | 스템 셀 앤드 리제너러티브 메디신 인터내셔날, 인크. | 기질 없는 조건 하에서 인간 배아줄기세포로부터 기능적 거핵구 및 혈소판의 대규모 생성 |
| SI2785359T1 (sl) | 2011-11-30 | 2018-11-30 | Astellas Institute For Regenerative Medicine | Mezenhimske stromalne celice in uporabe povezane z njimi |
| US8961956B2 (en) | 2011-11-30 | 2015-02-24 | Ocata Therapeutics, Inc. | Mesenchymal stromal cells and uses related thereto |
| GB201210857D0 (en) | 2012-06-19 | 2012-08-01 | Cambridge Entpr Ltd | Transcription factor mediated programming towards megakaryocytes |
| HK1208054A1 (en) | 2012-07-12 | 2016-02-19 | 爱姆斯坦生物技术公司 | Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof |
| US9074186B2 (en) | 2012-08-15 | 2015-07-07 | Boston Medical Center Corporation | Production of red blood cells and platelets from stem cells |
| CA2896053A1 (en) | 2012-12-21 | 2014-06-26 | Ocata Therapeutics, Inc. | Methods for production of platelets from pluripotent stem cells and compositions thereof |
| JP6580329B2 (ja) | 2014-03-31 | 2019-09-25 | シスメックス株式会社 | 未分化細胞から分化細胞および／または分化細胞の産生物を取得する方法 |
| US10538738B2 (en) | 2014-05-19 | 2020-01-21 | Eleftherios Papoutsakis | Megakaryocytic particles and microparticles for cell therapy and fate modification of stem and progenitor cells |
| US20180237797A1 (en) * | 2015-03-30 | 2018-08-23 | Jeffrey Thomas Loh | Methods for in vitro production of platelets and compositions and uses thereof |
| CN108697641A (zh) | 2015-08-18 | 2018-10-23 | 安斯泰来再生医药协会 | 临床制剂 |
| JP7287634B2 (ja) * | 2015-10-14 | 2023-06-06 | 株式会社メガカリオン | 精製血小板の製造方法 |
| JP2021509812A (ja) | 2018-01-05 | 2021-04-08 | プレートレット バイオジェネシス， インコーポレイテッド | 巨核球を産生するための組成物および方法 |
| EP3813853A4 (en) | 2018-06-29 | 2022-04-06 | Platelet Biogenesis, Inc. | DELIVERY COMPOSITIONS AND METHODS OF USE |
| US20230190815A1 (en) | 2020-05-11 | 2023-06-22 | Strm.Bio Incorporated | Compositions and methods related to megakaryocyte-derived extracellular vesicles |
| AU2022264498A1 (en) | 2021-04-26 | 2023-09-28 | Strm.Bio Incorporated | Compositions and methods related to megakaryocyte-derived extracellular vesicles for fanconi anemia |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5128259A (en) | 1989-10-27 | 1992-07-07 | Hahnemann University | Factor-dependent hematopoietic cell line exhibiting epo-induced erythrocyte maturation |
| EP1364197B1 (en) | 2001-01-29 | 2010-04-14 | Ivan N. Rich | ATP-based assay for the determination of the proliferation status of hematopoietic stem and progenitor cells |
| WO2002078449A2 (en) | 2001-04-02 | 2002-10-10 | Advanced Cell Technology, Inc. | Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells |
| MXPA04005010A (es) | 2001-11-26 | 2005-04-08 | Advanced Cell Tech Inc | METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS. |
| US20040052771A1 (en) | 2002-07-12 | 2004-03-18 | Lim Sai Kiang | Hemangioblast progenitor cells |
| US20040229350A1 (en) | 2003-05-12 | 2004-11-18 | Nikolai Strelchenko | Morula derived embryonic stem cells |
| CN1286970C (zh) | 2003-12-30 | 2006-11-29 | 上海伯瑞生物技术发展有限公司 | 由脐血cd34+细胞体外诱导生成巨核细胞的方法 |
| EP1735429A4 (en) | 2004-03-31 | 2008-06-11 | Newlink Genetics Corp | METHOD AND COMPOSITIONS FOR OBTAINING EMBRYONAL STEM CELLS OF ABSTINENT HEMATOPOETIC STEM CELLS AND USES THEREOF |
| AU2005302258B2 (en) * | 2004-11-01 | 2010-10-21 | Wisconsin Alumni Research Foundation | Platelets from stem cells |
| US7893315B2 (en) | 2004-11-04 | 2011-02-22 | Advanced Cell Technology, Inc. | Derivation of embryonic stem cells and embryo-derived cells |
| AU2007215276B2 (en) | 2006-02-09 | 2013-09-19 | Maryna E. Gumenyuk | Erythroid cells producing adult-type beta-hemoglobin generated from human embryonic stem cells |
| CN101045914A (zh) | 2006-03-29 | 2007-10-03 | 中国人民解放军军事医学科学院野战输血研究所 | 体外诱导造血干/祖细胞分化为成熟红细胞的方法与应用 |
| CN106434527A (zh) * | 2006-04-14 | 2017-02-22 | 安斯泰来再生医药协会 | 血管瘤集落形成细胞 |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| US9683232B2 (en) * | 2007-12-10 | 2017-06-20 | Kyoto University | Efficient method for nuclear reprogramming |
| CA3186451A1 (en) | 2008-05-06 | 2009-11-12 | Astellas Institute For Regenerative Medicine | Hemangio colony forming cells and non-engrafting hemangio cells |
| US20110086424A1 (en) | 2008-05-06 | 2011-04-14 | Advanced Cell Technology, Inc. | Methods for producing enucleated erythroid cells derived from pluripotent stem cells |
| KR20180086301A (ko) | 2009-12-04 | 2018-07-30 | 스템 셀 앤드 리제너러티브 메디신 인터내셔날, 인크. | 기질 없는 조건 하에서 인간 배아줄기세포로부터 기능적 거핵구 및 혈소판의 대규모 생성 |
-
2010
- 2010-12-03 KR KR1020187021160A patent/KR20180086301A/ko not_active Ceased
- 2010-12-03 CA CA2782013A patent/CA2782013C/en active Active
- 2010-12-03 KR KR1020197037186A patent/KR20190142424A/ko not_active Ceased
- 2010-12-03 WO PCT/US2010/058990 patent/WO2011069127A1/en not_active Ceased
- 2010-12-03 EP EP10835231.1A patent/EP2507359A4/en not_active Withdrawn
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| AU2019203670A1 (en) | 2019-06-13 |
| AU2022200188A1 (en) | 2022-02-10 |
| EP2507359A4 (en) | 2015-05-13 |
| CA2782013C (en) | 2021-06-08 |
| US20190002829A1 (en) | 2019-01-03 |
| US9988603B2 (en) | 2018-06-05 |
| CA2782013A1 (en) | 2011-06-09 |
| JP2022064953A (ja) | 2022-04-26 |
| KR20210066020A (ko) | 2021-06-04 |
| WO2011069127A1 (en) | 2011-06-09 |
| AU2017201995A1 (en) | 2017-04-13 |
| EP2507359A1 (en) | 2012-10-10 |
| KR20180086301A (ko) | 2018-07-30 |
| AU2017201995B2 (en) | 2019-06-06 |
| IN2012DN05054A (https=) | 2015-10-09 |
| KR20120128605A (ko) | 2012-11-27 |
| JP2016135138A (ja) | 2016-07-28 |
| JP2013512676A (ja) | 2013-04-18 |
| CA3115233A1 (en) | 2011-06-09 |
| AU2019203670B2 (en) | 2021-10-14 |
| KR20190142424A (ko) | 2019-12-26 |
| BR112012013497A2 (pt) | 2016-05-31 |
| JP2019122385A (ja) | 2019-07-25 |
| AU2010325811A1 (en) | 2012-06-21 |
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