RU2011114903A - Сепаратор для разделения, характеристики и(или) идентификации микроорганизмов - Google Patents
Сепаратор для разделения, характеристики и(или) идентификации микроорганизмов Download PDFInfo
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- RU2011114903A RU2011114903A RU2011114903/10A RU2011114903A RU2011114903A RU 2011114903 A RU2011114903 A RU 2011114903A RU 2011114903/10 A RU2011114903/10 A RU 2011114903/10A RU 2011114903 A RU2011114903 A RU 2011114903A RU 2011114903 A RU2011114903 A RU 2011114903A
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- 244000005700 microbiome Species 0.000 title claims abstract 5
- 238000000926 separation method Methods 0.000 title 1
- 230000009089 cytolysis Effects 0.000 claims abstract 7
- 229920000089 Cyclic olefin copolymer Polymers 0.000 claims abstract 4
- 230000003287 optical effect Effects 0.000 claims abstract 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract 4
- 239000004743 Polypropylene Substances 0.000 claims abstract 3
- 239000012528 membrane Substances 0.000 claims abstract 3
- -1 polypropylene Polymers 0.000 claims abstract 3
- 229920001155 polypropylene Polymers 0.000 claims abstract 3
- 239000004925 Acrylic resin Substances 0.000 claims abstract 2
- 229920000178 Acrylic resin Polymers 0.000 claims abstract 2
- 239000004713 Cyclic olefin copolymer Substances 0.000 claims abstract 2
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 claims abstract 2
- 239000010453 quartz Substances 0.000 claims abstract 2
- 229910052594 sapphire Inorganic materials 0.000 claims abstract 2
- 239000010980 sapphire Substances 0.000 claims abstract 2
- 239000013049 sediment Substances 0.000 claims abstract 2
- 238000001228 spectrum Methods 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims 2
- 239000012780 transparent material Substances 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 1
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Abstract
1. Контейнер для изоляции и идентификации микроорганизма, включающий:(a) верхнюю часть, имеющую широкий внутренний диаметр;(b) нижнюю часть, имеющую узкий внутренний диаметр; и(c) оптическое окно на дне, сверху и/или на одной или более чем одной стенке контейнера, которое является прозрачным по меньшей мере для части ближнего инфракрасного, видимого и/или ультрафиолетового светового спектра.2. Контейнер по п.1, который имеет объем, достаточный, чтобы удержать достаточное количество образца для получения осадка микроорганизмов.3. Контейнер по п.1, где верхняя часть и нижняя часть соединены средней конической частью.4. Контейнер по п.1, где нижняя часть составляет менее половины общей высоты контейнера.5. Контейнер по п.1, который включает устройство для закрывания или имеет резьбу для присоединения устройства для закрывания, так чтобы контейнер мог быть герметичным.6. Контейнер по п.1, где оптическое окно состоит из кварца, кварцевого стекла, сапфира, акриловой смолы, метакрилата, сополимера циклического олефина, полимера циклоолефина или любой их комбинации.7. Контейнер по п.1, который включает градиент плотности, содержащийся в нем.8. Контейнер по п.7, который дополнительно содержит селективный раствор для лизиса.9. Контейнер по п.8, который дополнительно включает тонкую мембрану, разделяющую градиент плотности и раствор для лизиса.10. Контейнер по п.7, который дополнительно включает полипропиленовый шарик.11. Одноразовый сепаратор, включающий:(a) контейнер цилиндрической формы, содержащий корпус, имеющий продольную ось, который определяет продольную внутреннюю капиллярную трубку, ориентированную вдоль оси, имеющую первый к
Claims (20)
1. Контейнер для изоляции и идентификации микроорганизма, включающий:
(a) верхнюю часть, имеющую широкий внутренний диаметр;
(b) нижнюю часть, имеющую узкий внутренний диаметр; и
(c) оптическое окно на дне, сверху и/или на одной или более чем одной стенке контейнера, которое является прозрачным по меньшей мере для части ближнего инфракрасного, видимого и/или ультрафиолетового светового спектра.
2. Контейнер по п.1, который имеет объем, достаточный, чтобы удержать достаточное количество образца для получения осадка микроорганизмов.
3. Контейнер по п.1, где верхняя часть и нижняя часть соединены средней конической частью.
4. Контейнер по п.1, где нижняя часть составляет менее половины общей высоты контейнера.
5. Контейнер по п.1, который включает устройство для закрывания или имеет резьбу для присоединения устройства для закрывания, так чтобы контейнер мог быть герметичным.
6. Контейнер по п.1, где оптическое окно состоит из кварца, кварцевого стекла, сапфира, акриловой смолы, метакрилата, сополимера циклического олефина, полимера циклоолефина или любой их комбинации.
7. Контейнер по п.1, который включает градиент плотности, содержащийся в нем.
8. Контейнер по п.7, который дополнительно содержит селективный раствор для лизиса.
9. Контейнер по п.8, который дополнительно включает тонкую мембрану, разделяющую градиент плотности и раствор для лизиса.
10. Контейнер по п.7, который дополнительно включает полипропиленовый шарик.
11. Одноразовый сепаратор, включающий:
(a) контейнер цилиндрической формы, содержащий корпус, имеющий продольную ось, который определяет продольную внутреннюю капиллярную трубку, ориентированную вдоль оси, имеющую первый конец и второй конец, где корпус дополнительно определяет резервуар, соединенный с первым концом капиллярной трубки;
(b) где корпус вблизи второго конца капиллярной трубки изготовлен из оптически прозрачного материала;
(c) крышку для резервуара для обеспечения доступа в резервуар, дающую возможность дозировать жидкий образец в резервуар.
12. Сепаратор по п.11, где его устройство дополнительно включает градиент плотности, содержащийся внутри резервуара.
13. Сепаратор по п.12, где его устройство дополнительно включает раствор для лизиса, наслоенный сверху на градиент плотности.
14. Сепаратор по п.13, где его устройство дополнительно включает тонкую мембрану, разделяющую градиент плотности и раствор для лизиса.
15. Сепаратор по п.11, где резервуар имеет вместимость жидкости от 0,5 до 15 мл.
16. Сепаратор по п.11, где его устройство дополнительно включает наконечник, прикрепленный к корпусу, который закрывает второй конец капиллярной трубки и который изготовлен из оптически прозрачного материала.
17. Сепаратор по п.11, где его устройство дополнительно включает коническую секцию, расположенную между резервуаром и указанной капиллярной трубкой.
18. Сепаратор по п.11, где контейнер включает градиент плотности, содержащийся в нем.
19. Сепаратор по п.11, где резервуар дополнительно содержит селективный раствор для лизиса.
20. Сепаратор по п.11, где резервуар дополнительно содержит полипропиленовый шарик.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11018708P | 2008-10-31 | 2008-10-31 | |
US61/110,187 | 2008-10-31 | ||
PCT/US2009/005888 WO2010062353A1 (en) | 2008-10-31 | 2009-10-30 | Separation device for use in the separation, characterization and/or identification of microorganisms |
Publications (2)
Publication Number | Publication Date |
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RU2011114903A true RU2011114903A (ru) | 2012-12-10 |
RU2510844C2 RU2510844C2 (ru) | 2014-04-10 |
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Application Number | Title | Priority Date | Filing Date |
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RU2011114903/10A RU2510844C2 (ru) | 2008-10-31 | 2009-10-30 | Контейнер для изоляции и идентификации микроорганизма |
Country Status (11)
Country | Link |
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US (1) | US20100120133A1 (ru) |
EP (1) | EP2364218B1 (ru) |
JP (3) | JP2012507284A (ru) |
KR (1) | KR101759995B1 (ru) |
CN (1) | CN102271814B (ru) |
AU (1) | AU2009320334B2 (ru) |
BR (1) | BRPI0920586A2 (ru) |
CA (1) | CA2741021C (ru) |
MX (1) | MX339825B (ru) |
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- 2009-10-30 JP JP2011534515A patent/JP2012507284A/ja active Pending
- 2009-10-30 RU RU2011114903/10A patent/RU2510844C2/ru not_active IP Right Cessation
- 2009-10-30 WO PCT/US2009/005888 patent/WO2010062353A1/en active Application Filing
- 2009-10-30 BR BRPI0920586A patent/BRPI0920586A2/pt not_active Application Discontinuation
- 2009-10-30 KR KR1020117012420A patent/KR101759995B1/ko active IP Right Grant
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- 2009-10-30 AU AU2009320334A patent/AU2009320334B2/en not_active Ceased
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- 2009-10-30 MX MX2011004172A patent/MX339825B/es active IP Right Grant
- 2009-10-30 CN CN200980153286.4A patent/CN102271814B/zh not_active Expired - Fee Related
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JP2015130878A (ja) | 2015-07-23 |
CN102271814A (zh) | 2011-12-07 |
AU2009320334A1 (en) | 2010-06-03 |
MX339825B (es) | 2016-06-13 |
RU2510844C2 (ru) | 2014-04-10 |
US20100120133A1 (en) | 2010-05-13 |
BRPI0920586A2 (pt) | 2017-05-30 |
EP2364218B1 (en) | 2020-04-15 |
CA2741021A1 (en) | 2010-06-03 |
EP2364218A1 (en) | 2011-09-14 |
CN102271814B (zh) | 2015-07-08 |
KR101759995B1 (ko) | 2017-07-31 |
JP6026581B2 (ja) | 2016-11-16 |
AU2009320334B2 (en) | 2015-05-07 |
WO2010062353A1 (en) | 2010-06-03 |
JP2015144607A (ja) | 2015-08-13 |
CA2741021C (en) | 2018-07-17 |
KR20110087309A (ko) | 2011-08-02 |
MX2011004172A (es) | 2011-09-27 |
JP6038214B2 (ja) | 2016-12-07 |
JP2012507284A (ja) | 2012-03-29 |
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