RU2011114903A - Сепаратор для разделения, характеристики и(или) идентификации микроорганизмов - Google Patents

Сепаратор для разделения, характеристики и(или) идентификации микроорганизмов Download PDF

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RU2011114903A
RU2011114903A RU2011114903/10A RU2011114903A RU2011114903A RU 2011114903 A RU2011114903 A RU 2011114903A RU 2011114903/10 A RU2011114903/10 A RU 2011114903/10A RU 2011114903 A RU2011114903 A RU 2011114903A RU 2011114903 A RU2011114903 A RU 2011114903A
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Джон УОЛШ
Джонс ХЬЮМЕН
Кристофер РОНСИК
Джон ЛИНК
Рон РОБИНСОН
Марк УИЛСОН
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Abstract

1. Контейнер для изоляции и идентификации микроорганизма, включающий:(a) верхнюю часть, имеющую широкий внутренний диаметр;(b) нижнюю часть, имеющую узкий внутренний диаметр; и(c) оптическое окно на дне, сверху и/или на одной или более чем одной стенке контейнера, которое является прозрачным по меньшей мере для части ближнего инфракрасного, видимого и/или ультрафиолетового светового спектра.2. Контейнер по п.1, который имеет объем, достаточный, чтобы удержать достаточное количество образца для получения осадка микроорганизмов.3. Контейнер по п.1, где верхняя часть и нижняя часть соединены средней конической частью.4. Контейнер по п.1, где нижняя часть составляет менее половины общей высоты контейнера.5. Контейнер по п.1, который включает устройство для закрывания или имеет резьбу для присоединения устройства для закрывания, так чтобы контейнер мог быть герметичным.6. Контейнер по п.1, где оптическое окно состоит из кварца, кварцевого стекла, сапфира, акриловой смолы, метакрилата, сополимера циклического олефина, полимера циклоолефина или любой их комбинации.7. Контейнер по п.1, который включает градиент плотности, содержащийся в нем.8. Контейнер по п.7, который дополнительно содержит селективный раствор для лизиса.9. Контейнер по п.8, который дополнительно включает тонкую мембрану, разделяющую градиент плотности и раствор для лизиса.10. Контейнер по п.7, который дополнительно включает полипропиленовый шарик.11. Одноразовый сепаратор, включающий:(a) контейнер цилиндрической формы, содержащий корпус, имеющий продольную ось, который определяет продольную внутреннюю капиллярную трубку, ориентированную вдоль оси, имеющую первый к

Claims (20)

1. Контейнер для изоляции и идентификации микроорганизма, включающий:
(a) верхнюю часть, имеющую широкий внутренний диаметр;
(b) нижнюю часть, имеющую узкий внутренний диаметр; и
(c) оптическое окно на дне, сверху и/или на одной или более чем одной стенке контейнера, которое является прозрачным по меньшей мере для части ближнего инфракрасного, видимого и/или ультрафиолетового светового спектра.
2. Контейнер по п.1, который имеет объем, достаточный, чтобы удержать достаточное количество образца для получения осадка микроорганизмов.
3. Контейнер по п.1, где верхняя часть и нижняя часть соединены средней конической частью.
4. Контейнер по п.1, где нижняя часть составляет менее половины общей высоты контейнера.
5. Контейнер по п.1, который включает устройство для закрывания или имеет резьбу для присоединения устройства для закрывания, так чтобы контейнер мог быть герметичным.
6. Контейнер по п.1, где оптическое окно состоит из кварца, кварцевого стекла, сапфира, акриловой смолы, метакрилата, сополимера циклического олефина, полимера циклоолефина или любой их комбинации.
7. Контейнер по п.1, который включает градиент плотности, содержащийся в нем.
8. Контейнер по п.7, который дополнительно содержит селективный раствор для лизиса.
9. Контейнер по п.8, который дополнительно включает тонкую мембрану, разделяющую градиент плотности и раствор для лизиса.
10. Контейнер по п.7, который дополнительно включает полипропиленовый шарик.
11. Одноразовый сепаратор, включающий:
(a) контейнер цилиндрической формы, содержащий корпус, имеющий продольную ось, который определяет продольную внутреннюю капиллярную трубку, ориентированную вдоль оси, имеющую первый конец и второй конец, где корпус дополнительно определяет резервуар, соединенный с первым концом капиллярной трубки;
(b) где корпус вблизи второго конца капиллярной трубки изготовлен из оптически прозрачного материала;
(c) крышку для резервуара для обеспечения доступа в резервуар, дающую возможность дозировать жидкий образец в резервуар.
12. Сепаратор по п.11, где его устройство дополнительно включает градиент плотности, содержащийся внутри резервуара.
13. Сепаратор по п.12, где его устройство дополнительно включает раствор для лизиса, наслоенный сверху на градиент плотности.
14. Сепаратор по п.13, где его устройство дополнительно включает тонкую мембрану, разделяющую градиент плотности и раствор для лизиса.
15. Сепаратор по п.11, где резервуар имеет вместимость жидкости от 0,5 до 15 мл.
16. Сепаратор по п.11, где его устройство дополнительно включает наконечник, прикрепленный к корпусу, который закрывает второй конец капиллярной трубки и который изготовлен из оптически прозрачного материала.
17. Сепаратор по п.11, где его устройство дополнительно включает коническую секцию, расположенную между резервуаром и указанной капиллярной трубкой.
18. Сепаратор по п.11, где контейнер включает градиент плотности, содержащийся в нем.
19. Сепаратор по п.11, где резервуар дополнительно содержит селективный раствор для лизиса.
20. Сепаратор по п.11, где резервуар дополнительно содержит полипропиленовый шарик.
RU2011114903/10A 2008-10-31 2009-10-30 Контейнер для изоляции и идентификации микроорганизма RU2510844C2 (ru)

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US11018708P 2008-10-31 2008-10-31
US61/110,187 2008-10-31
PCT/US2009/005888 WO2010062353A1 (en) 2008-10-31 2009-10-30 Separation device for use in the separation, characterization and/or identification of microorganisms

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