RU2008118836A - METHOD FOR DETECTING MYCOBACTERIA TUBERCULOSIS BEIJING GENOTYPE - Google Patents
METHOD FOR DETECTING MYCOBACTERIA TUBERCULOSIS BEIJING GENOTYPE Download PDFInfo
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- RU2008118836A RU2008118836A RU2008118836/13A RU2008118836A RU2008118836A RU 2008118836 A RU2008118836 A RU 2008118836A RU 2008118836/13 A RU2008118836/13 A RU 2008118836/13A RU 2008118836 A RU2008118836 A RU 2008118836A RU 2008118836 A RU2008118836 A RU 2008118836A
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- beijing genotype
- pcr
- genotype
- mycobacteria tuberculosis
- determining
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Abstract
Способ определения микобактерий туберкулеза генотипа Beijing путем определения специфических участков генома между соседними копиями элемента IS6110 посредством ПЦР, отличающийся тем, что применяют ПЦР с использованием двух инвертированных праймеров, расположенных на концах IS6110, и при амплификации фрагментов ДНК длиной 290 пн и 490 пн или 260 пн, 290 пн и 490 пн судят о принадлежности М. tuberculosis к «древнему» (атипичному) и «современному» (типичному) вариантам генотипа Beijing, соответственно.A method for determining the Mycobacterium tuberculosis of the Beijing genotype by determining specific genome regions between adjacent copies of the IS6110 element by PCR, characterized in that PCR is used using two inverted primers located at the ends of IS6110, and when amplifying DNA fragments of 290 bp and 490 bp or 260 bp length , 290 bp and 490 bp judge M. tuberculosis as belonging to the “ancient” (atypical) and “modern” (typical) variants of the Beijing genotype, respectively.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008118836/10A RU2405836C2 (en) | 2008-05-12 | 2008-05-12 | Method for mycobacterium tuberculosis beijing genotype detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008118836/10A RU2405836C2 (en) | 2008-05-12 | 2008-05-12 | Method for mycobacterium tuberculosis beijing genotype detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008118836A true RU2008118836A (en) | 2009-11-20 |
| RU2405836C2 RU2405836C2 (en) | 2010-12-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2008118836/10A RU2405836C2 (en) | 2008-05-12 | 2008-05-12 | Method for mycobacterium tuberculosis beijing genotype detection |
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| RU (1) | RU2405836C2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2485177C1 (en) * | 2012-03-07 | 2013-06-20 | Федеральное Государственное Бюджетное Учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН) | METHOD OF DETECTING RIFAMPICIN-RESISTANT ISOLATES Mycobacterium tuberculosis |
| CN108410971A (en) * | 2018-06-04 | 2018-08-17 | 上海市食品药品检验所 | A kind of bacterial nucleic acid sequencing identification method and bacterial identification kits based on DNA characteristics sequence |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2528866C2 (en) * | 2011-07-15 | 2014-09-20 | Федеральное государственное бюджетное учреждение "Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии" Министерства здравоохранения и социального развития Российской Федерации | Method for real-time detection of beijing genotype mycobacterium tuberculosis |
| RU2551764C2 (en) * | 2013-07-04 | 2015-05-27 | Федеральное государственное бюджетное учреждение науки Научно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства (ФГБУН НИИ ФХМ ФМБА России) | METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148 |
| RU2547598C1 (en) * | 2013-12-12 | 2015-04-10 | Автономная Некоммерческая Организация "Научно-исследовательский центр биотехнологии антибиотиков и других биотехнически активных веществ "БИОАН" | METHOD OF GENOTYPING STRAINS Mycobacterium tuberculosis |
| RU2743365C1 (en) * | 2020-05-12 | 2021-02-17 | Федеральное государственное бюджетное учреждение "Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии" Министерства здравоохранения Российской Федерации (ФГБУ "СПб НИИФ" Минздрава России) | Method of detecting phylogenetic sublines of beijing mycobacterium tuberculosis genotype in real time format |
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2008
- 2008-05-12 RU RU2008118836/10A patent/RU2405836C2/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2485177C1 (en) * | 2012-03-07 | 2013-06-20 | Федеральное Государственное Бюджетное Учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН) | METHOD OF DETECTING RIFAMPICIN-RESISTANT ISOLATES Mycobacterium tuberculosis |
| CN108410971A (en) * | 2018-06-04 | 2018-08-17 | 上海市食品药品检验所 | A kind of bacterial nucleic acid sequencing identification method and bacterial identification kits based on DNA characteristics sequence |
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| Publication number | Publication date |
|---|---|
| RU2405836C2 (en) | 2010-12-10 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20110513 |