RU2007449C1 - Strain of somatic structures of macroscopic fungus agaricus bisporus - a producer of bioactive substances - Google Patents

Abstract

FIELD: biotechnology. SUBSTANCE: strain Agaricus bisporus D49 is obtained from fruit body plates of meadow mushroom in the form of a pure culture. Strain is deposited in Microorganism Collection at No. 1. By cultivation of strain on solid and liquid nutrient media producer accumulates biomass (up to 27,3 g/l), vitamins C, PP and B-group, enzymes, - cellulase (83 U/g), amylase (55 U/g), protease (40-50 U/g), lipase (6-27 U/g), carboxylic acids (including sorbic, oxalic, fumaric, malic, tartaric, citric acids). Strain is used as a base for food addition for agricultural animals and poultry. EFFECT: enhanced accumulation of bioactive substances. 2 tbl

Description

 The invention relates to biotechnology and can be used as a producer of biologically active substances.

 Known industrial strains of champignon mushrooms, used mainly as producers of fruiting bodies. Close to the claimed object according to the habit of the fruiting body is the strain GDR-2 used in industry (state farm Zarechye, Moscow Region).

 The main disadvantage of this strain is that it slowly grows on liquid and solid nutrient media, which makes it inefficient to cultivate it in industry in order to obtain biomass and biologically active substances.

 The purpose of the invention is the expansion of the range of non-pathogenic strains producing biologically active substances, as well as accelerating the process of growing producer.

 A new strain of Agaricus bisporus mycelium was isolated from the plates of the fruit body of champignon, growing in the Balkhash district of Almaty region. Deposited in the collection of non-pathogenic microorganisms of the All-Russian Research Institute of Agricultural Microbiology VASKHNIL, Leningrad, room D 49.

 The strain has the following symptoms.

 Cultural and morphological. On wort agar, the culture forms velvety colonies from a dense layer of straight short hyphae of aerial mycelium. The edge of the colony is wavy, submerged, with a fluffy outer line, the reverse side of the colony is not painted. Aerial mycelium consists of well-developed generative and skeletal hyphae.

 Generative hyphae (thickness 2–3 μm), thin-walled, transparent, weakly branching, septated. Homogeneous protoplasm is located at partitions and hyphal growth points. The buckles are sharp. Skeletal hyphae are thick (4-6 microns), almost without partitions, there are swellings, a branch of generative hyphae. The protoplasm of skeletal hyphae is inlaid with irregular crystals.

Physiological and biochemical features - aerobe, bitimolny, pH 7.6, optimum growth temperature 18-20 ° ^C. At a higher and a lower temperature rise is depressed. The lower limit of the growth temperature is 13 ^o C.

 From carbon sources, the culture assimilates starch, maltose, arobinose, glucose.

 From sources of nitrogen, it is preferably absorbed by peptone, a yeast autolysate, worse mineral urea, ammonium sulfate, ammonium nitric acid, ammonium chloride.

 With a surface cultivation method on a grain substrate, the producer synthesizes the enzymes: cellulose, amylose, proteose, linase; carboxylic acids, mainly succinic, malic; B vitamins, PP, C vitamins, as well as lipids.

 EXAMPLE 1. Characterization of growth energy (growth coefficient).

Cultures of strains GDR-2 and the inventive surfactant seeded on a solid medium wort agar with chalk after 7 days of incubation at 22-28 ^{° C.} The following results were obtained (see Table 1..):
PRI me R 2. The productivity of the strain on liquid nutrient media. The culture was grown deep at 28-30 ^{° C} for 5 days on a shaker with 160 / min, the flask 100 ml of nutrient medium of the following composition, g / l:
1. Lamberg medium Acidic potassium phosphate 1.0 Ammonium nitrate 1.0 Magnesium sulfate 0.5 Sucrose 3.0 Glucose 1.0 Meltose 1.0 Autolysate 1.0 Water 1 l
2. Potato
200 g of peeled chopped potatoes are boiled for 30-40 minutes in 1 liter of water, the broth is filtered, the evaporated part of water and salt are added to it: Potassium phosphate 1.0 Ammonium nitrate 1.0 Magnesium sulfate 0.5 Sucrose 4.0 Glucose 1 , 0 3. Oat broth Oatmeal 30 g Water 970 ml 4. Carrot broth Carrot extract 400 ml Water 600 ml
N ote. The extract is prepared from crushed carrots, which are mixed with water in a ratio of 2: 5, boiled for 30 minutes, filtered (results in table. 2).

The usefulness or other effectiveness of the strain is due to its ability to produce biologically active substances, which will find application in agricultural practice, for example, as a feed additive, in the form of the following product:
The product obtained by solid-phase fermentation of wheat grain, Agaricus bisporus D49, has the following characteristics: Moisture 7-8% Crude fiber 5.5% Lipids 1-5% Vitamin C 32.8-34.2 mg / 100 g Thiamine (B ₁ ) 0.61-0.63 - "- Ribovlavin (B ₂ ) 0.32-0.37 -" - Niacin (PP) 8.0-8.5 - "-
Enzymes (units / g): Cellulose 82.5 Amylase 55.6-119.3 Protease 41.2-57.0 Lipase 6.0-27.0
Carboxylic acids 500-600 mg / 100 g (including sorbic, oxalic, fumaric, succinic, malic, ketoglutaric, tartaric, citric).

 The product is called BSh feed additive (Balkhash mushroom) and is proposed for farm animals. (56) Production of higher edible mushrooms in the USSR. 1978, 76-80.

Claims (1)

 The strain of somatic structures of the macroscopic fungus Agaricus bisporus - VNIISCHM - VIZR D-49 - producer of biologically active substances.

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