RS20060266A - Flp-mediated recombination - Google Patents

Flp-mediated recombination

Info

Publication number
RS20060266A
RS20060266A YUP-2006/0266A YUP20060266A RS20060266A RS 20060266 A RS20060266 A RS 20060266A YU P20060266 A YUP20060266 A YU P20060266A RS 20060266 A RS20060266 A RS 20060266A
Authority
RS
Serbia
Prior art keywords
flp
mediated recombination
host cell
polynucleotide
compositions
Prior art date
Application number
YUP-2006/0266A
Other languages
English (en)
Inventor
Holly Prentice
Louisa Caamano
Original Assignee
Biogen Idec Ma Inc.,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Idec Ma Inc., filed Critical Biogen Idec Ma Inc.,
Publication of RS20060266A publication Critical patent/RS20060266A/sr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Predmetni pronalazak obezbeđuje farmaceutske preparate i postupke korisne za homologu rekombinaciju i stabilnu integraciju polinukleotida u ćeliju dornaćina. Framaceutski preparati i postupci predmetnog pronalaska obezbeđuju brz i efikasan postupak za stabilno integrisanje egzogenog polipeptida u ćeliju domaćina, kao i za selekciju tako transformisanih ćelija.
YUP-2006/0266A 2003-10-14 2004-10-14 Flp-mediated recombination RS20060266A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51161003P 2003-10-14 2003-10-14
PCT/US2004/033868 WO2005038020A1 (en) 2003-10-14 2004-10-14 Flp-mediated recombination

Publications (1)

Publication Number Publication Date
RS20060266A true RS20060266A (en) 2008-09-29

Family

ID=34465252

Family Applications (1)

Application Number Title Priority Date Filing Date
YUP-2006/0266A RS20060266A (en) 2003-10-14 2004-10-14 Flp-mediated recombination

Country Status (17)

Country Link
US (1) US20070134795A1 (sr)
EP (1) EP1687418A4 (sr)
JP (1) JP2007508037A (sr)
KR (1) KR20060109906A (sr)
CN (1) CN1894402A (sr)
AU (1) AU2004282558A1 (sr)
BR (1) BRPI0415446A (sr)
CA (1) CA2542791A1 (sr)
EA (1) EA010059B1 (sr)
GE (1) GEP20094618B (sr)
IL (1) IL174892A0 (sr)
IS (1) IS8417A (sr)
MX (1) MXPA06004121A (sr)
NO (1) NO20062177L (sr)
RS (1) RS20060266A (sr)
WO (1) WO2005038020A1 (sr)
ZA (1) ZA200602984B (sr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104818253B (zh) * 2015-03-24 2018-03-09 成都贝爱特生物科技有限公司 靶向定点整合cho细胞系的改造及其用途
CN108441478A (zh) * 2017-02-16 2018-08-24 成都贝爱特生物科技有限公司 一种接受外源基因定点整合的基因工程细胞系
CN110914413A (zh) * 2017-02-17 2020-03-24 隆萨有限公司 产生腺相关病毒的哺乳动物细胞
EP3696271A4 (en) * 2017-10-11 2021-05-19 Celltrion Inc. EXPRESSION CASSETTE FOR THE PRODUCTION OF A HIGH EXPRESSION AND HIGH FUNCTIONALITY TARGET PROTEIN AND ITS USE

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
AU2001269709A1 (en) * 2000-07-19 2002-02-05 Eli Lilly And Company Nucleic acids, vectors, host cells, polypeptides, and uses thereof
CA2425852C (en) * 2000-10-13 2009-09-29 Chiron Corporation Cytomegalovirus intron a fragments
IL157746A0 (en) * 2001-05-30 2004-03-28 Chromos Molecular Systems Inc Chromosome-based platforms
AU2003283995A1 (en) * 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
AU2004213797B2 (en) * 2003-02-14 2009-09-17 Biogen Ma Inc. An expression cassette and vector for transient or stable expression of exogenous molecules

Also Published As

Publication number Publication date
EA010059B1 (ru) 2008-06-30
EP1687418A4 (en) 2007-07-18
MXPA06004121A (es) 2006-07-05
IS8417A (is) 2006-04-12
EA200600772A1 (ru) 2006-10-27
KR20060109906A (ko) 2006-10-23
EP1687418A1 (en) 2006-08-09
CN1894402A (zh) 2007-01-10
GEP20094618B (en) 2009-02-25
ZA200602984B (en) 2007-09-26
BRPI0415446A (pt) 2006-12-05
WO2005038020A1 (en) 2005-04-28
AU2004282558A1 (en) 2005-04-28
JP2007508037A (ja) 2007-04-05
NO20062177L (no) 2006-07-11
CA2542791A1 (en) 2005-04-28
US20070134795A1 (en) 2007-06-14
IL174892A0 (en) 2006-08-20

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