RO95943B1 - Procedeu de obtinere a aminoacilazei si proteazei - Google Patents
Procedeu de obtinere a aminoacilazei si proteazeiInfo
- Publication number
- RO95943B1 RO95943B1 RO124081A RO12408186A RO95943B1 RO 95943 B1 RO95943 B1 RO 95943B1 RO 124081 A RO124081 A RO 124081A RO 12408186 A RO12408186 A RO 12408186A RO 95943 B1 RO95943 B1 RO 95943B1
- Authority
- RO
- Romania
- Prior art keywords
- aminoacylase
- protease
- enzymes
- distilled water
- obtaining process
- Prior art date
Links
- ULGJWNIHLSLQPZ-UHFFFAOYSA-N 7-[(6,8-dichloro-1,2,3,4-tetrahydroacridin-9-yl)amino]-n-[2-(1h-indol-3-yl)ethyl]heptanamide Chemical compound C1CCCC2=NC3=CC(Cl)=CC(Cl)=C3C(NCCCCCCC(=O)NCCC=3C4=CC=CC=C4NC=3)=C21 ULGJWNIHLSLQPZ-UHFFFAOYSA-N 0.000 title abstract 3
- 108091005804 Peptidases Proteins 0.000 title abstract 3
- 239000004365 Protease Substances 0.000 title abstract 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 3
- 108010003977 aminoacylase I Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 239000012153 distilled water Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 240000006439 Aspergillus oryzae Species 0.000 abstract 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract 1
- 229920005654 Sephadex Polymers 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000005227 gel permeation chromatography Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 235000010755 mineral Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 235000009566 rice Nutrition 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 235000015099 wheat brans Nutrition 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
Procedeul de obtinere a aminoacilazei si proteazei, conform inventiei, consta în aceea ca microorganismului Aspergillus oryzae, înregistrat în colectia Institutului "dr. I.Cantacuzino ", Bucuresti la nr.108/73, se cultiva pe un mediu de cultura constituit din coji de orez, tarâte de grâu, saruri minerale alese dintre fosfat monopotasic si clorura de cobalt si apa distilata, la pH = 9 si temperatura de 28 degree C timp de 80 ... 100 h, dupa care biomasa obtinuta este amestecata cu apa distilata în raport 1 : 10, se filtreaza si din extractul obtinut se separa cele doua enzime prin precipitare fractionata cu sulfat de amoniu la concentratia 70% pentru aminoacilaza si 90 % pentru proteaza, solutiile enzimatice obtinute sunt trecute printr-o coloana cromatografica cu gel de tip dextran reticulat si apoi concentrate în vid la temperatura de 25 degree C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO124081A RO95943B1 (ro) | 1986-07-10 | 1986-07-10 | Procedeu de obtinere a aminoacilazei si proteazei |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO124081A RO95943B1 (ro) | 1986-07-10 | 1986-07-10 | Procedeu de obtinere a aminoacilazei si proteazei |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RO95943B1 true RO95943B1 (ro) | 1988-09-17 |
Family
ID=40904964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO124081A RO95943B1 (ro) | 1986-07-10 | 1986-07-10 | Procedeu de obtinere a aminoacilazei si proteazei |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO95943B1 (ro) |
-
1986
- 1986-07-10 RO RO124081A patent/RO95943B1/ro unknown
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