PT3440205T - Diagnóstico não invasivo através da sequenciação de dna 5- hidroximetilado sem células - Google Patents

Diagnóstico não invasivo através da sequenciação de dna 5- hidroximetilado sem células

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Publication number
PT3440205T
PT3440205T PT177795937T PT17779593T PT3440205T PT 3440205 T PT3440205 T PT 3440205T PT 177795937 T PT177795937 T PT 177795937T PT 17779593 T PT17779593 T PT 17779593T PT 3440205 T PT3440205 T PT 3440205T
Authority
PT
Portugal
Prior art keywords
sequencing
free dna
hydroxymethylated
cell
noninvasive diagnostics
Prior art date
Application number
PT177795937T
Other languages
English (en)
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of PT3440205T publication Critical patent/PT3440205T/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/04—Methods of creating libraries, e.g. combinatorial synthesis using dynamic combinatorial chemistry techniques
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
PT177795937T 2016-04-07 2017-04-03 Diagnóstico não invasivo através da sequenciação de dna 5- hidroximetilado sem células PT3440205T (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662319702P 2016-04-07 2016-04-07
US201762444122P 2017-01-09 2017-01-09
US201762461712P 2017-02-21 2017-02-21

Publications (1)

Publication Number Publication Date
PT3440205T true PT3440205T (pt) 2021-08-06

Family

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Family Applications (1)

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PT177795937T PT3440205T (pt) 2016-04-07 2017-04-03 Diagnóstico não invasivo através da sequenciação de dna 5- hidroximetilado sem células

Country Status (14)

Country Link
US (7) US10718010B2 (pt)
EP (2) EP3440205B1 (pt)
JP (2) JP7143221B2 (pt)
CN (1) CN109312399B (pt)
AU (1) AU2017246318B2 (pt)
CA (1) CA3019836A1 (pt)
DK (1) DK3440205T3 (pt)
ES (1) ES2882329T3 (pt)
MX (1) MX2018012156A (pt)
PL (1) PL3440205T3 (pt)
PT (1) PT3440205T (pt)
RU (1) RU2742355C2 (pt)
SG (1) SG11201808775PA (pt)
WO (1) WO2017176630A1 (pt)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
EP3425065B1 (en) 2011-12-13 2021-04-21 Oslo Universitetssykehus HF Methods and kits for detection of methylation status
US10563248B2 (en) 2012-11-30 2020-02-18 Cambridge Epigenetix Limited Oxidizing agent for modified nucleotides
CA2912666A1 (en) 2013-05-28 2014-12-04 Ramot At Tel-Aviv University Ltd. Detection of hydroxymethylcytosine bases
EP3355939A4 (en) 2015-09-30 2019-04-17 Trustees of Boston University MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES
CA3080117A1 (en) 2017-10-27 2019-05-02 Juno Diagnostics, Inc. Devices, systems and methods for ultra-low volume liquid biopsy
CN115181783A (zh) 2018-01-08 2022-10-14 路德维格癌症研究院 胞嘧啶修饰的免亚硫酸氢盐的碱基分辨率鉴定
EP3752515A1 (en) 2018-02-14 2020-12-23 Bluestar Genomics, Inc. Methods for the epigenetic analysis of dna, particularly cell-free dna
JP2021514663A (ja) * 2018-03-08 2021-06-17 セント・ジョーンズ・ユニバーシティＳｔ． Ｊｏｈｎｓ Ｕｎｉｖｅｒｓｉｔｙ 循環性血清無細胞ｄｎａバイオマーカー及び方法
EP3810806A1 (en) 2018-06-22 2021-04-28 Bluestar Genomics, Inc. Hydroxymethylation analysis of cell-free nucleic acid samples for assigning tissue of origin, and related methods of use
JP2022501033A (ja) * 2018-09-19 2022-01-06 ブルースター ジェノミクス， インコーポレイテッド 膵臓病変の評価における無細胞ｄｎａヒドロキシメチル化プロファイル
EP3856903A4 (en) 2018-09-27 2022-07-27 Grail, LLC METHYLATION MARKER AND TARGETED METHYLATION PROBE PANEL
CN113166796A (zh) 2018-10-04 2021-07-23 蓝星基因组股份有限公司 来自单个生物样品的蛋白质、核糖体和无细胞核酸的同时基于测序的分析
JP2022505327A (ja) * 2018-10-19 2022-01-14 コリア リサーチ インスティチュート オブ バイオサイエンス アンド バイオテクノロジー Ｓｙｔ１１抑制剤を有効成分として含む胃癌治療用組成物
CN109321647A (zh) * 2018-10-26 2019-02-12 苏州森苗生物科技有限公司 标记组合物及羟甲基化核酸文库的构建方法
WO2020194057A1 (en) * 2019-03-22 2020-10-01 Cambridge Epigenetix Limited Biomarkers for disease detection
CN113906146A (zh) * 2019-03-27 2022-01-07 朱诺诊断学公司 优化的超低体积液体活检方法、系统和设备
EP3990548A4 (en) * 2019-06-25 2023-07-26 AccuraGen Holdings Limited DISEASE DETECTION METHODS AND SYSTEMS
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
WO2021198726A1 (en) * 2020-03-30 2021-10-07 Vilnius University Methods and compositions for noninvasive prenatal diagnosis through targeted covalent labeling of genomic sites
CA3187549A1 (en) 2020-07-30 2022-02-03 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
AU2021329899A1 (en) 2020-08-19 2023-03-09 Exact Sciences Corporation Detecting non-hodgkin lymphoma
JP2024506854A (ja) 2021-01-29 2024-02-15 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 複数の種類のがんの有無の検出
CN113528616A (zh) * 2021-07-26 2021-10-22 深圳泰莱生物科技有限公司 一种捕获cfDNA5hmC片段的检测方法
CN115992203A (zh) * 2022-07-26 2023-04-21 生工生物工程（上海）股份有限公司 一种全基因组羟甲基化捕获测序的文库构建方法

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US20100273151A1 (en) * 2004-05-28 2010-10-28 Fred Hutchinson Cancer Research Center Genome-wide analysis of palindrome formation and dna methylation
MX2007015416A (es) * 2005-06-08 2008-02-19 Millennium Pharm Inc Metodos para la identificacion, evaluacion y tratamiento de pacientes con terapia contra cancer.
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US20090105959A1 (en) 2007-06-01 2009-04-23 Braverman Michael S System and method for identification of individual samples from a multiplex mixture
US20090093378A1 (en) 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
EP2340314B8 (en) * 2008-10-22 2015-02-18 Illumina, Inc. Preservation of information related to genomic dna methylation
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
EP2765205B1 (en) * 2009-04-01 2016-05-18 DxTerity Diagnostics Incorporated Chemical ligation dependent probe amplification (CLPA)
EP2470675B1 (en) * 2009-08-25 2016-03-30 New England Biolabs, Inc. Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
DE112011100297B4 (de) * 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
WO2011127136A1 (en) 2010-04-06 2011-10-13 University Of Chicago Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc)
US20120034603A1 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
WO2012038839A2 (en) 2010-09-21 2012-03-29 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
US20120122087A1 (en) 2010-11-17 2012-05-17 Weiwei Li 5-Hydroxymethylcytosine as a biomarker for early detection, treatment and prognostic monitoring of cancer
EP2694686B2 (en) 2011-04-06 2023-07-19 The University of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
EP2697397B1 (en) 2011-04-15 2017-04-05 The Johns Hopkins University Safe sequencing system
RS61631B1 (sr) 2012-02-17 2021-04-29 Hutchinson Fred Cancer Res Kompozicije i postupci za preciznu identifikaciju mutacija
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US9732390B2 (en) * 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
EP2922989B1 (en) 2012-11-26 2018-04-04 The University of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
EP3597774A1 (en) * 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
WO2015021282A1 (en) * 2013-08-09 2015-02-12 New England Biolabs, Inc. Detecting, sequencing and/or mapping 5-hydroxymethylcytosine and 5-formylcytosine at single-base resolution
US9932623B2 (en) * 2013-08-19 2018-04-03 Abbott Molecular Inc. Nucleotide analogs
CN106103743A (zh) * 2014-01-07 2016-11-09 Imppc私人基金会 用于产生双链dna文库的方法和用于鉴定甲基化胞嘧啶的测序方法
WO2015116837A1 (en) * 2014-01-30 2015-08-06 The Regents Of The University Of California Methylation haplotyping for non-invasive diagnosis (monod)
GB201405226D0 (en) * 2014-03-24 2014-05-07 Cambridge Entpr Ltd Nucleic acid preparation method
PT3889271T (pt) * 2014-06-06 2022-12-20 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas
US20180046754A1 (en) * 2015-03-24 2018-02-15 Agency For Science, Technology And Research (A*Star) Normalization methods for measuring gene copy number and expression
US11162139B2 (en) 2016-03-02 2021-11-02 Shanghai Epican Genetech Co. Ltd. Method for genomic profiling of DNA 5-methylcytosine and 5-hydroxymethylcytosine

Also Published As

Publication number Publication date
ES2882329T3 (es) 2021-12-01
US10718010B2 (en) 2020-07-21
CA3019836A1 (en) 2017-10-12
EP3440205A4 (en) 2019-04-03
RU2742355C2 (ru) 2021-02-05
US20200277666A1 (en) 2020-09-03
JP7143221B2 (ja) 2022-09-28
EP3440205A1 (en) 2019-02-13
PL3440205T3 (pl) 2021-11-22
SG11201808775PA (en) 2018-11-29
CN109312399A (zh) 2019-02-05
RU2018138848A (ru) 2020-05-12
US20200248248A1 (en) 2020-08-06
JP2019520791A (ja) 2019-07-25
DK3440205T3 (da) 2021-08-16
WO2017176630A1 (en) 2017-10-12
US20190017109A1 (en) 2019-01-17
RU2018138848A3 (pt) 2020-12-15
CN109312399B (zh) 2023-02-03
AU2017246318A1 (en) 2018-11-08
US20200248249A1 (en) 2020-08-06
US20200277667A1 (en) 2020-09-03
US20200283838A1 (en) 2020-09-10
US20200299760A1 (en) 2020-09-24
MX2018012156A (es) 2019-02-07
JP2022120007A (ja) 2022-08-17
AU2017246318B2 (en) 2023-07-27
AU2017246318A2 (en) 2018-11-08
EP3440205B1 (en) 2021-05-26
EP3929290A1 (en) 2021-12-29

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