PT3064575T - Método para diferenciar células estaminais pluripotentes induzidas a partir de células estaminais mesenquimais em hepatócitos - Google Patents

Método para diferenciar células estaminais pluripotentes induzidas a partir de células estaminais mesenquimais em hepatócitos

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Publication number
PT3064575T
PT3064575T PT138968383T PT13896838T PT3064575T PT 3064575 T PT3064575 T PT 3064575T PT 138968383 T PT138968383 T PT 138968383T PT 13896838 T PT13896838 T PT 13896838T PT 3064575 T PT3064575 T PT 3064575T
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PT
Portugal
Prior art keywords
stem cell
hepatocyte
differentiating pluripotent
induced
mesenchymal stem
Prior art date
Application number
PT138968383T
Other languages
English (en)
Original Assignee
Bbhc Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Bbhc Co Ltd filed Critical Bbhc Co Ltd
Publication of PT3064575T publication Critical patent/PT3064575T/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C12N2502/137—Blood-borne mesenchymal stem cells, e.g. Msc from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1369—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PT138968383T 2013-11-01 2013-11-05 Método para diferenciar células estaminais pluripotentes induzidas a partir de células estaminais mesenquimais em hepatócitos PT3064575T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020130132058A KR101542849B1 (ko) 2013-11-01 2013-11-01 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 간세포로 분화시키는 방법

Publications (1)

Publication Number Publication Date
PT3064575T true PT3064575T (pt) 2020-04-02

Family

ID=53004390

Family Applications (1)

Application Number Title Priority Date Filing Date
PT138968383T PT3064575T (pt) 2013-11-01 2013-11-05 Método para diferenciar células estaminais pluripotentes induzidas a partir de células estaminais mesenquimais em hepatócitos

Country Status (17)

Country Link
US (1) US10072248B2 (pt)
EP (1) EP3064575B1 (pt)
JP (1) JP6711757B2 (pt)
KR (1) KR101542849B1 (pt)
CN (1) CN105683359B (pt)
AU (1) AU2013403885B2 (pt)
CY (1) CY1122791T1 (pt)
DK (1) DK3064575T3 (pt)
ES (1) ES2776183T3 (pt)
HR (1) HRP20200474T1 (pt)
HU (1) HUE048804T2 (pt)
LT (1) LT3064575T (pt)
PL (1) PL3064575T3 (pt)
PT (1) PT3064575T (pt)
RS (1) RS60363B1 (pt)
SI (1) SI3064575T1 (pt)
WO (1) WO2015064802A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015064795A1 (ko) 2013-11-01 2015-05-07 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포
KR101699761B1 (ko) * 2014-05-23 2017-01-25 주식회사 비비에이치씨 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법
KR102018783B1 (ko) * 2015-08-13 2019-09-06 (주) 넥셀 간세포의 약물대사 기능 향상방법
CN105385651B (zh) * 2015-12-11 2019-06-14 湖南光琇高新生命科技有限公司 诱导性多能干细胞定向诱导分化为肝细胞的方法及肝细胞
KR20180057546A (ko) 2016-11-21 2018-05-30 성균관대학교산학협력단 지방줄기세포유래 엑소좀을 유효성분으로 포함하는 간 섬유증 예방 또는 치료용 조성물
WO2018093233A1 (ko) 2016-11-21 2018-05-24 성균관대학교산학협력단 지방줄기세포유래 엑소좀을 유효성분으로 포함하는 간 섬유증 예방 또는 치료용 조성물
WO2020013719A1 (en) * 2018-07-10 2020-01-16 Uniwersytet Jagielloński Stem cell culture method using plant secretion
EP3878432A4 (en) 2018-12-14 2022-04-27 Thothscience Inc. PHARMACEUTICAL COMPOSITION FOR THE PREVENTION OR TREATMENT OF HEPATIC DISEASE, COMPRISING A BIOIMPLANT COMPRISING MESENCHYMATOUS STEM CELLS
JP2023516484A (ja) 2020-03-11 2023-04-19 ビット バイオ リミテッド 肝細胞作製方法

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US20100135970A1 (en) * 2006-10-27 2010-06-03 Caritas St. Elizabeth Medical Center Of Boston, In Methods for Reprogramming Adult Somatic Cells and Uses Thereof
KR20100001940A (ko) 2008-06-28 2010-01-06 최송화 마우스 내장용 키보드
WO2010014949A2 (en) 2008-07-31 2010-02-04 The General Hospital Corporation Compositions comprising hepatocyte-like cells and uses thereof
KR101016761B1 (ko) 2009-02-26 2011-02-25 부경대학교 산학협력단 피부 미백활성을 갖는 감태 추출물
KR101073523B1 (ko) 2009-03-10 2011-10-17 (주)나우이앤씨 비개착식 터널 굴착 방법 및 이에 사용되는 터널시공구조체
EP2457998A4 (en) * 2009-07-23 2013-08-21 Beijing Huayuanbochuang Technology Co Ltd METHODS FOR OBTAINING HEPATIC CELLS, HEPATIC ENDODERM CELLS, AND HEPATIC PRECURSOR CELLS BY INDUCING THE DIFFERENTIATION
JP2012210154A (ja) * 2009-08-03 2012-11-01 Keio Gijuku 分化細胞由来多能性幹細胞の樹立方法
WO2011037301A1 (ko) * 2009-09-22 2011-03-31 서울대학교병원 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포
KR20110079436A (ko) 2010-03-29 2011-07-07 (주) 세이텍 오토틸트형 휴대폰 슬라이딩 힌지
KR20120040488A (ko) 2010-10-19 2012-04-27 충남대학교산학협력단 곰피와 감태 추출물 유래의 플로로탄닌을 포함하는 화장품 조성물
KR101887956B1 (ko) 2011-08-10 2018-08-13 (주)아모레퍼시픽 효소 처리 감태 추출물을 포함하는 피부 미백용 조성물
CN102286532B (zh) * 2011-09-05 2012-10-17 浙江大学 一种获得诱导性多能干细胞的方法
KR101408106B1 (ko) 2011-12-30 2014-06-19 박영준 줄기세포 분화능 개선용 조성물

Also Published As

Publication number Publication date
CY1122791T1 (el) 2021-05-05
HUE048804T2 (hu) 2020-09-28
KR20150050864A (ko) 2015-05-11
CN105683359A (zh) 2016-06-15
RS60363B1 (sr) 2020-07-31
EP3064575A4 (en) 2017-07-26
JP2016537022A (ja) 2016-12-01
PL3064575T3 (pl) 2020-08-10
HRP20200474T1 (hr) 2020-10-02
KR101542849B1 (ko) 2015-08-10
DK3064575T3 (da) 2020-03-16
JP6711757B2 (ja) 2020-06-17
EP3064575B1 (en) 2020-02-26
SI3064575T1 (sl) 2020-08-31
US10072248B2 (en) 2018-09-11
AU2013403885B2 (en) 2018-04-05
EP3064575A1 (en) 2016-09-07
AU2013403885A1 (en) 2016-06-23
ES2776183T3 (es) 2020-07-29
LT3064575T (lt) 2020-06-10
US20160272936A1 (en) 2016-09-22
WO2015064802A1 (ko) 2015-05-07
CN105683359B (zh) 2021-08-03

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