PT2265729E - Amplificação e sequenciação alvo com iniciadores compreendendo unidades de monómeros que formam triplexes - Google Patents
Amplificação e sequenciação alvo com iniciadores compreendendo unidades de monómeros que formam triplexes Download PDFInfo
- Publication number
- PT2265729E PT2265729E PT97194070T PT09719407T PT2265729E PT 2265729 E PT2265729 E PT 2265729E PT 97194070 T PT97194070 T PT 97194070T PT 09719407 T PT09719407 T PT 09719407T PT 2265729 E PT2265729 E PT 2265729E
- Authority
- PT
- Portugal
- Prior art keywords
- oligonucleotides
- forming monomer
- triplex forming
- primers
- sequencing
- Prior art date
Links
- 239000000178 monomer Substances 0.000 title abstract 3
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 4
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 230000008018 melting Effects 0.000 abstract 1
- 238000002844 melting Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200800366 | 2008-03-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT2265729E true PT2265729E (pt) | 2013-10-10 |
Family
ID=40886885
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT97194070T PT2265729E (pt) | 2008-03-10 | 2009-03-10 | Amplificação e sequenciação alvo com iniciadores compreendendo unidades de monómeros que formam triplexes |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US8501441B2 (da) |
| EP (1) | EP2265729B1 (da) |
| JP (1) | JP5285090B2 (da) |
| KR (1) | KR101289164B1 (da) |
| CN (1) | CN102016076A (da) |
| AU (1) | AU2009225119B2 (da) |
| BR (1) | BRPI0909063A2 (da) |
| CA (1) | CA2717994C (da) |
| DK (1) | DK2265729T3 (da) |
| ES (1) | ES2434542T3 (da) |
| NZ (1) | NZ588041A (da) |
| PT (1) | PT2265729E (da) |
| WO (1) | WO2009112032A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110130557A1 (en) * | 2008-05-07 | 2011-06-02 | Erik Bjerregaard Pedersen | Intercalating triplexes and duplexes using aryl naphthoimidazol and process for the preparation thereof |
| EP2566981A2 (en) * | 2010-05-07 | 2013-03-13 | Quantibact A/s | Method for generating a double stranded nucleic acid with a single stranded overhang |
| KR20140043891A (ko) * | 2011-03-28 | 2014-04-11 | ?티백트 에이/에스 | 스태킹 핵산 및 이의 사용 방법 |
| WO2013097868A1 (en) | 2011-12-27 | 2013-07-04 | Region Syddanmark | Detection of adenomas of the colon or rectum |
| KR101589483B1 (ko) * | 2014-02-21 | 2016-01-28 | 디엑스진주식회사 | 핵산과 신호 프로브의 비대칭 등온증폭을 이용한 핵산의 검출방법 |
| JP6762296B2 (ja) | 2014-10-02 | 2020-09-30 | ヴェンタナ メディカル システムズ， インク． | ポリマー及びポリマーを含むコンジュゲート |
| GB2582561B (en) * | 2019-03-22 | 2023-05-24 | Ev Offshore Ltd | Downhole imaging |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602005018840D1 (de) | 2005-04-14 | 2010-02-25 | Applied Biosystems Llc | 3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sonden |
| US9102937B2 (en) | 2005-05-25 | 2015-08-11 | Tina Holding Aps | Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (TINA) and process for the preparation of TINA |
-
2009
- 2009-03-10 WO PCT/DK2009/050051 patent/WO2009112032A1/en not_active Ceased
- 2009-03-10 CN CN2009801167150A patent/CN102016076A/zh active Pending
- 2009-03-10 CA CA2717994A patent/CA2717994C/en not_active Expired - Fee Related
- 2009-03-10 BR BRPI0909063-0A patent/BRPI0909063A2/pt not_active IP Right Cessation
- 2009-03-10 DK DK09719407.0T patent/DK2265729T3/da active
- 2009-03-10 NZ NZ588041A patent/NZ588041A/xx not_active IP Right Cessation
- 2009-03-10 AU AU2009225119A patent/AU2009225119B2/en not_active Ceased
- 2009-03-10 US US12/921,561 patent/US8501441B2/en not_active Expired - Fee Related
- 2009-03-10 ES ES09719407T patent/ES2434542T3/es active Active
- 2009-03-10 PT PT97194070T patent/PT2265729E/pt unknown
- 2009-03-10 JP JP2010550029A patent/JP5285090B2/ja not_active Expired - Fee Related
- 2009-03-10 KR KR1020107022708A patent/KR101289164B1/ko not_active Expired - Fee Related
- 2009-03-10 EP EP09719407.0A patent/EP2265729B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| NZ588041A (en) | 2013-01-25 |
| EP2265729B1 (en) | 2013-07-03 |
| DK2265729T3 (da) | 2013-10-14 |
| CN102016076A (zh) | 2011-04-13 |
| KR101289164B1 (ko) | 2013-07-31 |
| US8501441B2 (en) | 2013-08-06 |
| WO2009112032A1 (en) | 2009-09-17 |
| JP5285090B2 (ja) | 2013-09-11 |
| BRPI0909063A2 (pt) | 2015-08-25 |
| AU2009225119B2 (en) | 2012-11-15 |
| CA2717994A1 (en) | 2009-09-17 |
| KR20100123904A (ko) | 2010-11-25 |
| EP2265729A1 (en) | 2010-12-29 |
| US20110076728A1 (en) | 2011-03-31 |
| ES2434542T3 (es) | 2013-12-16 |
| JP2011512850A (ja) | 2011-04-28 |
| CA2717994C (en) | 2014-09-16 |
| AU2009225119A1 (en) | 2009-09-17 |
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